Grape ASR-Silencing Sways Nuclear Proteome, Histone Marks and Interplay of Intrinsically Disordered Proteins by Hristo Atanassov, Jonathan Parrilla, Caroline Artault, Jérémy Verbeke, Thomas Schneider, Jonas Grossmann, Bernd Roschitzki, Rossitza Atanassova

“…The most significantly up- or down-regulated nuclear proteins were involved in epigenetic regulation, DNA replication/repair, transcription, mRNA splicing/stability/editing, rRNA processing/biogenesis, metabolism, cell division/differentiation and stress responses. …”
Get full text

m6 RNA methylation: an emerging common target in the immune response to cancer and severe acute respiratory syndrome-coronavirus-2 infection by Hiromichi Sato, Tomoaki Hara, Chihiro Otsuka, Yasuko Arao, Yoshiko Tsuji, Yumiko Hamano, Mirei Ogita, Eric di Luccio, Takaaki Hirotsu, Andrea Vecchione, Hideshi Ishii

“…m6A RNA methylation, a predominant type of RNA modification, is involved in regulating mRNA splicing, stability, and translation as well as the interaction between nucleoproteins and noncoding RNAs. …”
Get full text

Spotting the Targets of the Apospory Controller <i>TGS1</i> in <i>Paspalum notatum</i> by Carolina Marta Colono, Maricel Podio, Lorena Adelina Siena, Juan Pablo A. Ortiz, Olivier Leblanc, Silvina Claudia Pessino

“…In the subtropical grass <i>Paspalum notatum</i>, one of the controllers of apospory, a main component of gametophytic apomixis reproduction, is <i>TRIMETHYLGUANOSINE SYNTHASE 1</i> (<i>TGS1</i>), a multifunctional gene previously associated with RNA cleavage regulation (including mRNA splicing as well as rRNA and miRNA processing), transcriptional modulation and the establishment of heterochromatin. …”
Get full text

Pan‐cancer analyses of bromodomain containing 9 as a novel therapeutic target reveals its diagnostic, prognostic potential and biological mechanism in human tumours by Yu Chen, Zitong Gao, Isam Mohd‐Ibrahim, Hua Yang, Lang Wu, Yuanyuan Fu, Youping Deng

“…Analysis of survival data and DNA methylation for BRD9 indicated distinct conclusions for multiple tumours. mRNA splicing and molecular binding were involved in the functional mechanism of BRD9. …”
Get full text

Challenges in Diagnosing Intermediate Maple Syrup Urine Disease by Newborn Screening and Functional Validation of Genomic Results Imperative for Reproductive Family Planning by Mona Sajeev, Sharon Chin, Gladys Ho, Bruce Bennetts, Bindu Parayil Sankaran, Bea Gutierrez, Beena Devanapalli, Adviye Ayper Tolun, Veronica Wiley, Janice Fletcher, Maria Fuller, Shanti Balasubramaniam

“…Previously reported mRNA splicing studies supported a deleterious effect of the c.434-15_434-4del variant. …”
Get full text

Impaired expression of serine/arginine protein kinase 2 (SRPK2) affects melanoma progression by Mônica Maria Magalhães Caetano, Gabriela Alves Moreira, Maria Roméria da Silva, Gabriela Rapozo Guimarães, Leandro de Oliveira Santos, Amanda de Ambrósio Pacheco, Raoni Pais Siqueira, Flávia Carneiro Mendes, Eduardo De Almeida Marques Da Silva, Abelardo Silva Junior, Juliana Lopes Rangel Fietto, Ângela Saito, Mariana Boroni, Gustavo Costa Bressan

“…The serine/arginine protein kinases 1 and 2 (SRPK1 and SRPK2) are classically related to the control of pre-mRNA splicing through SR protein phosphorylation and have been found overexpressed in many types of cancer, including melanoma. …”
Get full text

The RNA helicase DDX3 and its role in c-MYC driven germinal center-derived B-cell lymphoma by Marion Lacroix, Marion Lacroix, Hugues Beauchemin, Cyrus Khandanpour, Tarik Möröy, Tarik Möröy, Tarik Möröy

“…DDX3X is an RNA helicase with many functions in RNA metabolism such as mRNA translation, alternative pre-mRNA splicing and mRNA stability, but also plays a role as a regulator of transcription as well as in the Wnt/beta-catenin- and Nf-κB signaling pathways. …”
Get full text

GOLPH3 modulates expression and alternative splicing of transcription factors associated with endometrial decidualization in human endometrial stromal cells by Suqin Zhu, Dianliang Lin, Zhoujie Ye, Xiaojing Chen, Wenwen Jiang, Huiling Xu, Song Quan, Beihong Zheng

“…Through functional cluster analysis of these DEGs and genes where differential alternative splicing events are located, it is found that they are enriched in the PI3K/Akt signaling pathway, RNA splicing and processing, transcription factors and other pathways related to endometrial decidualization and important biological processes, indicating the important biological function of GOLPH3. …”
Get full text

Single‐cell atlas of peripheral blood mononuclear cells from pregnant women by Dongsheng Chen, Wei Wang, Linlin Wu, Langchao Liang, Shiyou Wang, Yunfeng Cheng, Tongda Zhang, Chaochao Chai, Qiuhong Luo, Chengcheng Sun, Wandong Zhao, Zhiyuan Lv, Ya Gao, Xiaoxia Wu, Ning Sun, Yiwei Zhang, Jing Zhang, Yixuan Chen, Jianing Tong, Xiangdong Wang, Yong Bai, Chaoyang Sun, Xin Jin, Jianmin Niu

“…Results We identified reconfiguration of the peripheral immune cell phenotype during pregnancy, including interferon‐stimulated gene upregulation, activation of RNA splicing‐related pathways and immune activity of cell subpopulations. …”
Get full text

Identification of tumor antigens and immune landscapes for bladder urothelial carcinoma mRNA vaccine by Zhuolun Sun, Changying Jing, Changying Jing, Hailun Zhan, Xudong Guo, Ning Suo, Feng Kong, Wen Tao, Chutian Xiao, Daoyuan Hu, Hanbo Wang, Shaobo Jiang

“…We aimed to identify potential effective antigens of BLCA for the development of mRNA-based vaccines and screen for immune clusters to select appropriate candidates for vaccination.MethodsGene expression microarray data and clinical information were retrieved from The Cancer Genome Atlas and GSE32894, respectively. The mRNA splicing patterns were obtained from the SpliceSeq portal. …”
Get full text

Genome-Wide Screening and Stability Verification of the Robust Internal Control Genes for RT-qPCR in Filamentous Fungi by Yayong Yang, Xinyu Xu, Zhuohan Jing, Jun Ye, Hui Li, Xiaoyu Li, Lei Shi, Mengyu Chen, Tengyun Wang, Baogui Xie, Yongxin Tao

“…This study screened 6 novel ICGs: Pre-mRNA-splicing factor cwc15 (<i>Cwf15</i>); ER associated DnaJ chaperone (<i>DnaJ</i>); E3 ubiquitin-protein ligase NEDD4 (<i>HUL4</i>); ATP-binding cassette, subfamily B (MDR/TAP), member 1 (<i>VAMP</i>); Exosome complex exonuclease DIS3/RRP44 (<i>RNB</i>); V-type H⁺-transporting ATPase sub-unit A (<i>V-ATP</i>) from the 22-transcriptome data of 8 filamentous fungi. …”
Get full text

Salidroside ameliorates memory impairment following long-term ethanol intake in rats by modulating the altered intestinal microbiota content and hippocampal gene expression by Yu Jiao, Zhenglin Zhao, Xin Li, Lulu Li, Dan Xiao, Dan Xiao, Siyuan Wan, Tong Wu, Tong Li, Ping Li, Rongjie Zhao

“…And, enrichment analyses revealed these genes were related to the structural components of the ribosome, mRNA splicing process, protein translation, mitochondria function, and immunological reaction. …”
Get full text

SRSF1 promotes proliferation, invasion and migration of esophageal squamous cell carcinoma Eca9706 cells by regulating VEGFA mRNA alternative splicing by DUAN Yuqing, XIA Ning, JIA Yunlong, ZHENG Wenya, LIU Lihua

“…Conclusion: SRSF1 can promote proliferation, invasion and migration of ESCC Eca9706 cells by possibly regulating VEGFA mRNA splicing.…”
Get full text

An Update of Lectins from Marine Organisms: Characterization, Extraction Methodology, and Potential Biofunctional Applications by Mirja Kaizer Ahmmed, Shuva Bhowmik, Stephen G. Giteru, Md. Nazmul Hasan Zilani, Parise Adadi, Shikder Saiful Islam, Osman N. Kanwugu, Monjurul Haq, Fatema Ahmmed, Charlene Cheuk Wing Ng, Yau Sang Chan, Md. Asadujjaman, Gabriel Hoi Huen Chan, Ryno Naude, Alaa El-Din Ahmed Bekhit, Tzi Bun Ng, Jack Ho Wong

“…Lectins can also control cell proliferation, protein folding, RNA splicing, and trafficking of molecules. Due to their reported biological and pharmaceutical activities, lectins have attracted the attention of scientists and industries (i.e., food, biomedical, and pharmaceutical industries). …”
Get full text

Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice by Longjie Sun, Zheng Lv, Xuexue Chen, Rong Ye, Shuang Tian, Chaofan Wang, Xiaomei Xie, Lu Yan, Xiaohong Yao, Yujing Shao, Sheng Cui, Juan Chen, Jiali Liu

“…In addition, immunoprecipitation mass spectrometry and co-immunoprecipitation data showed that SRSF1 interacts with RNA splicing-related proteins (e.g. SART1, RBM15, and SRSF10). …”
Get full text

Identification of the Mtus1 Splice Variant as a Novel Inhibitory Factor Against Cardiac Hypertrophy by Shin Ito, Masanori Asakura, Yulin Liao, Kyung‐Duk Min, Ayako Takahashi, Kazuhiro Shindo, Satoru Yamazaki, Osamu Tsukamoto, Hiroshi Asanuma, Masaki Mogi, Masatsugu Horiuchi, Yoshihiro Asano, Shoji Sanada, Tetsuo Minamino, Seiji Takashima, Naoki Mochizuki, Masafumi Kitakaze

“…BackgroundIn cardiac hypertrophy and failure, there is a widespread alteration in mRNA splicing, but the role of splice variants in cardiac hypertrophy has not yet been fully elucidated. …”
Get full text

Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses by Shuang Ding, Haibin Liu, Lijuan Liu, Li Ma, Zhen Chen, Miao Zhu, Lishi Liu, Xueyan Zhang, Haojie Hao, Li Zuo, Jingwen Yang, Xiulin Wu, Ping Zhou, Fang Huang, Fan Zhu, Wuxiang Guan

“…Abstract Eukaryotic five-methylcytosine (m5C) is an important regulator of viral RNA splicing, stability, and translation. However, its role in HBV replication remains largely unknown. …”
Get full text

Splicing Modulation as a Promising Therapeutic Strategy for Lysosomal Storage Disorders: The Mucopolysaccharidoses Example by Juliana Inês Santos, Mariana Gonçalves, Liliana Matos, Luciana Moreira, Sofia Carvalho, Maria João Prata, Maria Francisca Coutinho, Sandra Alves

“…With more than 600 mutations that cause disease affecting the pre-mRNA splicing process, we consider lysosomal storage diseases (LSDs) to be perfect candidates for this type of approach. …”
Get full text

Characterisation of a novel mouse model of mitochondrial disease – a hypomorphic Wars2 ENU-induced allele by Agnew, T

“…</p> <p>The <em>Wars2</em>V117L/V117L allele disrupts <em>Wars2</em> mRNA splicing, resulting in reduced full-length <em>Wars2</em> (Wars2-FL) mRNA in <em>Wars2^V117L/V117L</em> tissues and cells. …”

Omics Analysis of Educated Platelets in Cancer and Benign Disease of the Pancreas by Giulia Mantini, Laura L. Meijer, Ilias Glogovitis, Sjors G. J. G. In ‘t Veld, Rosita Paleckyte, Mjriam Capula, Tessa Y. S. Le Large, Luca Morelli, Thang V. Pham, Sander R. Piersma, Adam E. Frampton, Connie R. Jimenez, Geert Kazemier, Danijela Koppers-Lalic, Thomas Wurdinger, Elisa Giovannetti

“…Gene ontology mining revealed enrichment of RNA splicing, mRNA processing and translation initiation in miRNAs and proteins but depletion in RNA transcripts. …”
Get full text