Jmjd6 catalyses lysyl-hydroxylation of U2AF65, a protein associated with RNA splicing. by Webby, C, Wolf, A, Gromak, N, Dreger, M, Kramer, H, Kessler, B, Nielsen, M, Schmitz, C, Butler, D, Yates, JR, Delahunty, C, Hahn, P, Lengeling, A, Mann, M, Proudfoot, N, Schofield, C, Böttger, A

“…Jmjd6 is shown to change alternative RNA splicing of some, but not all, of the endogenous and reporter genes, supporting a specific role for Jmjd6 in the regulation of RNA splicing.…”

Thirty Years with ERH: An mRNA Splicing and Mitosis Factor Only or Rather a Novel Genome Integrity Protector? by Piotr Kozlowski

“…Finally, studies in humans pointed to a role in pre-mRNA splicing and in mitosis but further research, also in <i>Caenorhabditis elegans</i> and <i>Schizosaccharomyces pombe,</i> demonstrated its much broader activity, namely involvement in the biogenesis of mRNA, and miRNA, piRNA and some other ncRNAs, and in repressive heterochromatin formation. …”
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Modification of BCLX pre-mRNA splicing has antitumor efficacy alone or in combination with radiotherapy in human glioblastoma cells by Zhihui Dou, Huiwen Lei, Wei Su, Taotao Zhang, Xiaohua Chen, Boyi Yu, Xiaogang Zhen, Jing Si, Chao Sun, Hong Zhang, Cuixia Di

“…Modulation of BCLX pre-mRNA splicing using splice-switching oligonucleotides (SSOs) efficiently elevated the pro-apoptotic isoform Bcl-xS at the expense of the anti-apoptotic Bcl-xL. …”
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Hybrid Minigene Assay: An Efficient Tool to Characterize mRNA Splicing Profiles of <i>NF1</i> Variants by Valeria Morbidoni, Elisa Baschiera, Monica Forzan, Valentina Fumini, Dario Seif Ali, Gianpietro Giorgi, Lisa Buson, Maria Andrea Desbats, Matteo Cassina, Maurizio Clementi, Leonardo Salviati, Eva Trevisson

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The endogenous HBZ interactome in ATL leukemic cells reveals an unprecedented complexity of host interacting partners involved in RNA splicing by Mariam Shallak, Tiziana Alberio, Mauro Fasano, Maria Monti, Maria Monti, Ilaria Iacobucci, Ilaria Iacobucci, Julien Ladet, Franck Mortreux, Roberto S. Accolla, Greta Forlani

“…Here we analyzed in detail the cluster involved in RNA splicing. RNAseq analysis showed that HBZ specifically altered the transcription of many genes, including crucial oncogenes, by affecting different splicing events. …”
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DHX38 restricts chemoresistance by regulating the alternative pre-mRNA splicing of RELL2 in pancreatic ductal adenocarcinoma. by Zeru Li, Cheng Qin, Bangbo Zhao, Yuanyang Wang, Tianyu Li, Yutong Zhao, Weibin Wang

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The calcium channel TRPC6 promotes chemotherapy-induced persistence by regulating integrin α6 mRNA splicing by Dimpi Mukhopadhyay, Hira Lal Goel, Choua Xiong, Shivam Goel, Ayush Kumar, Rui Li, Lihua Julie Zhu, Jennifer L. Clark, Michael A. Brehm, Arthur M. Mercurio

“…These data reveal a Ca2+-dependent mechanism of chemotherapy-induced persistence, which is amenable to therapy, that involves integrin mRNA splicing.…”
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Altered HIV-1 mRNA Splicing Due to Drug-Resistance-Associated Mutations in Exon 2/2b by Lisa Müller, Wiebke Moskorz, Anna-Lena Brillen, Frank Hillebrand, Philipp Niklas Ostermann, Niklas Kiel, Lara Walotka, Johannes Ptok, Jörg Timm, Nadine Lübke, Heiner Schaal

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AtMC1 Associates With LSM4 to Regulate Plant Immunity Through Modulating Pre-mRNA Splicing by Shune Wang, Mei Xue, Chan He, Danyu Shen, Chunhao Jiang, Hongwei Zhao, Dongdong Niu

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CircSMARCA5 Regulates VEGFA mRNA Splicing and Angiogenesis in Glioblastoma Multiforme Through the Binding of SRSF1 by Davide Barbagallo, Angela Caponnetto, Duilia Brex, Federica Mirabella, Cristina Barbagallo, Giovanni Lauretta, Antonio Morrone, Francesco Certo, Giuseppe Broggi, Rosario Caltabiano, Giuseppe M. Barbagallo, Vittoria Spina-Purrello, Marco Ragusa, Cinzia Di Pietro, Thomas B. Hansen, Michele Purrello

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Characterization of alternative mRNA splicing in cultured cell populations representing progressive stages of human fetal kidney development by Yishay Wineberg, Itamar Kanter, Nissim Ben-Haim, Naomi Pode-Shakked, Efrat Bucris, Tali Hana Bar-Lev, Sarit Oriel, Harel Reinus, Yishai Yehuda, Rotem Gershon, Rachel Shukrun, Dekel Dov Bar-Lev, Achia Urbach, Benjamin Dekel, Tomer Kalisky

“…RNA binding motif enrichment analysis suggests that the mRNA binding proteins ESRP1, ESRP2, RBFOX2, and QKI regulate alternative mRNA splicing during human kidney development. These findings illuminate new molecular mechanisms involved in human kidney development and pediatric kidney cancer.…”
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Mechanism underlying PTBP1-mediated alternative pre-mRNA splicing in regulating tissue-specific gene expression by Muhammad Fursham Hamid

“…Alternative pre-mRNA splicing (AS) provides an efficient means for diversifying the proteome and regulating gene expression levels in the higher eukaryotes. …”
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Identification of a BRCA1-mRNA Splicing Complex Required for Efficient DNA Repair and Maintenance of Genomic Stability by Savage, K, Gorski, J, Barros, E, Irwin, G, Manti, L, Powell, A, Pellagatti, A, Lukashchuk, N, McCance, D, McCluggage, W, McCluggage, W, Schettino, G, Schettino, G, Salto-Tellez, M, Boultwood, J, Richard, D, McDade, S, Harkin, D

“…Here, we report the identification of a DNA damage-induced BRCA1 protein complex containing BCLAF1 and other key components of the mRNA-splicing machinery. In response to DNA damage, this complex regulates pre-mRNA splicing of a number of genes involved in DNA damage signaling and repair, thereby promoting the stability of these transcripts/proteins. …”

Two catalytic subunits of cAMP-dependent protein kinase generated by alternative RNA splicing are expressed in Aplysia neurons. by Beushausen, S, Bergold, P, Sturner, S, Elste, A, Roytenberg, V, Schwartz, J, Bayley, H

Identification of a BRCA1-mRNA splicing complex required for efficient DNA repair and maintenance of genomic stability. by Savage, K, Gorski, J, Barros, E, Irwin, G, Manti, L, Powell, A, Pellagatti, A, Lukashchuk, N, McCance, D, McCluggage, W, Schettino, G, Salto-Tellez, M, Boultwood, J, Richard, D, McDade, S, Harkin, D

“…Here, we report the identification of a DNA damage-induced BRCA1 protein complex containing BCLAF1 and other key components of the mRNA-splicing machinery. In response to DNA damage, this complex regulates pre-mRNA splicing of a number of genes involved in DNA damage signaling and repair, thereby promoting the stability of these transcripts/proteins. …”

LARD: a new lymphoid-specific death domain containing receptor regulated by alternative pre-mRNA splicing. by Screaton, G, Xu, X, Olsen, A, Cowper, A, Tan, R, McMichael, A, Bell, J

“…The expression of LARD is more tightly regulated than that of either Fas or TNF-R1 as it is found predominantly on lymphocytes (T and B cells) but not on macrophages or a number of transformed lymphocyte cell lines. Alternative pre-mRNA splicing generates at least 11 distinct isoforms of LARD. …”

SON connects the splicing-regulatory network with pluripotency in human embryonic stem cells by Lu, Xinyi, Göke, Jonathan, Sachs, Friedrich, Jacques, Pierre-Étienne, Liang, Hongqing, Feng, Bo, Bourque, Guillaume, Bubulya, Paula A., Ng, Huck-Hui

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A novel MMUT splicing variant causing mild methylmalonic acidemia phenotype by Xinjie Zhang, Xiaowei Xu, Jianbo Shu, Xiufang Zhi, Hong Wang, Yan Dong, Wenchao Sheng, Dong Li, Yingtao Meng, Chunquan Cai

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Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity by Riccardo Guidi, Christopher Wedeles, Daqi Xu, Krzysztof Kolmus, Sarah E. Headland, Grace Teng, Joseph Guillory, Yi Jimmy Zeng, Tommy K. Cheung, Subhra Chaudhuri, Zora Modrusan, Yuxin Liang, Stuart Horswell, Benjamin Haley, Sascha Rutz, Christopher Rose, Yvonne Franke, Donald S. Kirkpatrick, Jason A. Hackney, Mark S. Wilson

“…Instead, AGO1/3/4 regulate the expansion of type 2 immunity via precursor mRNA splicing in CD4+ T helper (Th) lymphocytes. Gain- and loss-of-function experiments demonstrate that nuclear AGO3 interacts directly with SF3B3, a component of the U2 spliceosome complex, to aid global mRNA splicing, and in particular the isoforms of the gene Nisch, resulting in a dysregulated Nisch isoform ratio. …”
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Alternative mRNA Splicing Controls the Functions of the Histone H3K27 Demethylase UTX/KDM6A by Omid Fotouhi, Sheikh Nizamuddin, Stephanie Falk, Oliver Schilling, Ruth Knüchel-Clarke, Martin L. Biniossek, H. T. Marc Timmers

“…Despite many studies on UTX proteins, variations in mRNA splicing have been overlooked. Using Nanopore sequencing, we present a comprehensive analysis of UTX/KDM6A splicing events in human cell lines and in tissue samples from bladder cancer cases and normal epithelia. …”
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