Analysis workflow of publicly available RNA-sequencing datasets by Pablo Sanchis, Rosario Lavignolle, Mercedes Abbate, Sofía Lage-Vickers, Elba Vazquez, Javier Cotignola, Juan Bizzotto, Geraldine Gueron

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Annotating cell types in human single-cell RNA-seq data with CellO by Matthew N. Bernstein, Colin N. Dewey

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Exploring the RNA Gap for Improving Diagnostic Yield in Primary Immunodeficiencies by Jed J. Lye, Anthony Williams, Anthony Williams, Diana Baralle, Diana Baralle

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Dissociation and inDrops microfluidic encapsulation of human gut tissues for single-cell atlasing studies by Alan J. Simmons, Ken S. Lau

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RNAseq Reveals Novel and Differentially Expressed Isoforms In Native and Commercial Poultry by Ayeh Sadat Sadr, Mohammadreza Nassiri, Seyed Alireza Salami, Mohammad Reza Bakhtiarizadeh, Mojtaba Tahmoorespur, Alireza Shafeinia

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Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging by Xinyue Lu, Qiuzhong Zhou, Jin Liu, Lei Sun

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Protocol to benchmark gene expression signature scoring techniques for single-cell RNA sequencing data in cancer by Nighat Noureen, Xiaojing Wang, Siyuan Zheng

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Integrated transcriptomics contrasts fatty acid metabolism with hypoxia response in β-cell subpopulations associated with glycemic control by Mario A Miranda, Juan F Macias-Velasco, Heather Schmidt, Heather A Lawson

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Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material by Gordon Wiegleb, Susanne Reinhardt, Andreas Dahl, Nico Posnien

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Application of high-throughput 5′P sequencing for the study of co-translational mRNA decay by Yujie Zhang, Vicent Pelechano

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Aberrant CpG‐methylation affects genes expression predicting survival in lung adenocarcinoma by Wei He, Dandan Ju, Zhijun Jie, Ai Zhang, Xin Xing, Qin Yang

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Differential regulation of gene expression pathways with dexamethasone and ACTH after early life seizures by Jeffrey L. Brabec, Mohamed Ouardouz, J. Matthew Mahoney, Rod C. Scott, Amanda E. Hernan

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Protocol to analyze dysregulation of the eIF4F complex in human cancers using R software and large public datasets by Su Wu, Gerhard Wagner

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Genome-wide gene expression changes of Pseudomonas veronii 1YdBTEX2 during bioaugmentation in polluted soils by Marian Morales, Vladimir Sentchilo, Noushin Hadadi, Jan Roelof van der Meer

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Spatial gene expression in the adult rat patellar tendon by Danielle Steffen, Michael Mienaltowski, Keith Baar

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Profiling low-mRNA content cells in complex human tissues using BD Rhapsody single-cell analysis by Alexandra Scheiber, Manuel Trebo, Annabella Pittl, Isabel Heidegger, Theresa Hautz, Rupert Oberhuber, Zlatko Trajanoski, Florian Augustin, Sieghart Sopper, Dominik Wolf, Andreas Pircher, Stefan Salcher

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Comprehensive guide for epigenetics and transcriptomics data quality control by Arianna Comendul, Frederique Ruf-Zamojski, Colby T. Ford, Pankaj Agarwal, Elena Zaslavsky, German Nudelman, Manoj Hariharan, Aliza Rubenstein, Hanna Pincas, Venugopalan D. Nair, Adam M. Michaleas, Philip D. Fremont-Smith, Darrell O. Ricke, Stuart C. Sealfon, Christopher W. Woods, Kajal T. Claypool, Rafael Jaimes, III

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Micro-RNAs differentially expressed in comparative analysis of sperm samples with high and low efficiency in the in vitro production of bovine (Bos Taurus) embryos by Ricardo Tomaz da Silva, Matheus de Souza Gomes, Patrícia Tiemi Fujimura, Carlos Ueira-Vieira, Laurence Rodrigues do Amaral, Marcelo Emílio Beletti

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Protocol for profiling in vitro intratumor heterogeneity using spatially annotated single-cell sequencing by Myrthe M. Smit, Kate J. Feller, Li You, Miao-Ping Chien

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RNA extraction protocol from low-biomass bacterial Nitrosomonas europaea and Nitrobacter winogradskyi cultures for whole transcriptome studies by Tom Verbeelen, Rob Van Houdt, Natalie Leys, Ramon Ganigué, Felice Mastroleo

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