Chromatin Organization after High-LET Irradiation Revealed by Super-Resolution STED Microscopy by Benjamin Schwarz, Nicole Matejka, Sarah Rudigkeit, Matthias Sammer, Judith Reindl

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Exploring the Release of Toxic Oligomers from α-Synuclein Fibrils with Antibodies and STED Microscopy by Alessandra Bigi, Emilio Ermini, Serene W. Chen, Roberta Cascella, Cristina Cecchi

“…This study investigated the uptake by neuronal cells of pathologically relevant αS oligomers and fibrils exploiting a range of conformation-sensitive antibodies, and the super-resolution stimulated emission depletion (STED) microscopy. We found that prefibrillar oligomers promptly penetrate neuronal membranes, thus resulting in cell dysfunction. …”
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2PE-STED microscopy with a single Ti:sapphire laser for reduced illumination. by Qifeng Li, Yang Wang, Da Chen, Sherry S H Wu

“…We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. …”
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Three-dimensional super-resolution high-throughput imaging by structured illumination STED microscopy by Xue, Yi, So, Peter T. C.

“…Stimulated emission depletion (STED) microscopy is able to image fluorescence labeled samples with nanometer scale resolution. …”
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Molecular recognition of the native HIV-1 MPER revealed by STED microscopy of single virions by Carravilla, P, Chojnacki, J, Rujas, E, Insausti, S, Largo, E, Waithe, D, Apellaniz, B, Sicard, T, Julien, J, Eggeling, C, Nieva, J

“…Here, accessibility of antibodies to the native Env MPER on single virions has been addressed through STED microscopy. STED imaging of fluorescently labeled Fabs reveals a common pattern of native Env recognition for HIV-1 antibodies targeting MPER or the surface subunit gp120. …”

Studying nanoscale membrane dynamics by sub-diffraction far-field fluorescence STED microscopy by Eggeling, C, Ringemann, C, Mueller, V, Medda, R, Schwarzmann, G, Sandhoff, K, Hein, B, Schoenle, A, Hell, S

Fusion of clathrin and caveolae endocytic vesicles revealed by line-switching dual-color STED microscopy by Hefei Ruan, Jianqiang Yu, Yayun Wu, Xiaojun Tang, Jinghe Yuan, Xiaohong Fang

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Visualization of early influenza A virus trafficking in human dendritic cells using STED microscopy. by Faezzah Baharom, Oliver S Thomas, Rico Lepzien, Ira Mellman, Cécile Chalouni, Anna Smed-Sörensen

“…Using three-color Stimulated Emission Depletion (STED) microscopy, we visualized input IAV nucleoprotein (NP), early and late endosomal compartments (EEA1 and LAMP1 respectively), and HLA-DR (DC membrane/cytosol) by immunofluorescence in human DCs. …”
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New Insight into Lipid-Protein Membrane Organization and its Functionality with Super-Resolution STED Microscopy by Eggeling, C, Mueller, V, Honigmann, A, Andrade, D, de la Serna, J, Hell, S

A general method for three color STED microscopy with one depletion laser : application to primary neuronal culture by Lee, Mackenzie C.,S.M.Massachusetts Institute of Technology.

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ExSTED microscopy reveals contrasting functions of dopamine and somatostatin CSF-c neurons along the lamprey central canal by Elham Jalalvand, Jonatan Alvelid, Giovanna Coceano, Steven Edwards, Brita Robertson, Sten Grillner, Ilaria Testa

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Density and function of actin-microdomains in healthy and NF1 deficient osteoclasts revealed by combined use of AFM and STED-microscopy by Takahiro Deguchi, Elnaz Fazeli, Sami Koho, Paula Pennanen, Maria Alanne, Mayank Modi, John Eriksson, Kari Vienola, Pekka Hänninen, Juha Peltonen, Tuomas Näreoja

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Spatial distribution of Na⁺-K⁺-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy by Bondar Alexander, Widengren Jerker, Spicarova Zuzana, Scott Lena, Rönnlund Daniel, Blom Hans, Aperia Anita, Brismar Hjalmar

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The interwoven fibril-like structure of amyloid-beta plaques in mouse brain tissue visualized using super-resolution STED microscopy by Björn Johansson, Sho Oasa, Aida Muntsant Soria, Ann Tiiman, Linda Söderberg, Ebba Amandius, Christer Möller, Lars Lannfelt, Lars Terenius, Lydia Giménez-Llort, Vladana Vukojević

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All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging by Hirokazu Ishii, Kohei Otomo, Ching-Pu Chang, Miwako Yamasaki, Masahiko Watanabe, Hiroyuki Yokoyama, Tomomi Nemoto

“…In this study, we developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time-gated fluorescence detection, namely, all-pulsed 2PE-gSTED microscopy. The implementation of time-gating is critical to excluding undesirable signals derived from brain tissues. …”
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All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging. by Hirokazu Ishii, Kohei Otomo, Ching-Pu Chang, Miwako Yamasaki, Masahiko Watanabe, Hiroyuki Yokoyama, Tomomi Nemoto

“…In this study, we developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time-gated fluorescence detection, namely, all-pulsed 2PE-gSTED microscopy. The implementation of time-gating is critical to excluding undesirable signals derived from brain tissues. …”
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Super-Resolution STED Microscopy-Based Mobility Studies of the Viral Env Protein at HIV-1 Assembly Sites of Fully Infected T-Cells by Jakub Chojnacki, Christian Eggeling

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STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue. by Honigmann, A, Mueller, V, Hell, S, Eggeling, C

“…The same ternary mixture deposited on acid-cleaned glass forms Lo nanodomains of < 40 nm to 300 nm in diameter as only revealed by STED microscopy, which demonstrates the severe influence of interactions with the substrate on the sizes of domains in membranes. …”

Specific Lipid Recruitment by the Retroviral Gag Protein upon HIV-1 Assembly: From Model Membranes to Infected Cells by Cyril Favard, Jakub Chojnacki, Naresh Yandrapalli, Johnson Mak, Christian Eggeling, Delphine Muriaux

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Cellular Mechanisms of Etrolizumab Treatment in Inflammatory Bowel Disease by Charlotte Lichnog, Sha Klabunde, Emily Becker, Franklin Fuh, Philipp Tripal, Raja Atreya, Entcho Klenske, Rich Erickson, Henry Chiu, Chae Reed, Shan Chung, Clemens Neufert, Imke Atreya, Jacqueline McBride, Markus F. Neurath, Sebastian Zundler

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