All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging. by Hirokazu Ishii, Kohei Otomo, Ching-Pu Chang, Miwako Yamasaki, Masahiko Watanabe, Hiroyuki Yokoyama, Tomomi Nemoto

“…In this study, we developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time-gated fluorescence detection, namely, all-pulsed 2PE-gSTED microscopy. The implementation of time-gating is critical to excluding undesirable signals derived from brain tissues. …”
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Super-Resolution STED Microscopy-Based Mobility Studies of the Viral Env Protein at HIV-1 Assembly Sites of Fully Infected T-Cells by Jakub Chojnacki, Christian Eggeling

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STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue. by Honigmann, A, Mueller, V, Hell, S, Eggeling, C

“…The same ternary mixture deposited on acid-cleaned glass forms Lo nanodomains of < 40 nm to 300 nm in diameter as only revealed by STED microscopy, which demonstrates the severe influence of interactions with the substrate on the sizes of domains in membranes. …”

Specific Lipid Recruitment by the Retroviral Gag Protein upon HIV-1 Assembly: From Model Membranes to Infected Cells by Cyril Favard, Jakub Chojnacki, Naresh Yandrapalli, Johnson Mak, Christian Eggeling, Delphine Muriaux

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Cellular Mechanisms of Etrolizumab Treatment in Inflammatory Bowel Disease by Charlotte Lichnog, Sha Klabunde, Emily Becker, Franklin Fuh, Philipp Tripal, Raja Atreya, Entcho Klenske, Rich Erickson, Henry Chiu, Chae Reed, Shan Chung, Clemens Neufert, Imke Atreya, Jacqueline McBride, Markus F. Neurath, Sebastian Zundler

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Dynamic Mode Decomposition of Multiphoton and Stimulated Emission Depletion Microscopy Data for Analysis of Fluorescent Probes in Cellular Membranes by Daniel Wüstner, Jacob Marcus Egebjerg, Line Lauritsen

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A Method to Generate Vector Beams with Adjustable Amplitude in the Focal Plane by Alexandru Crăciun, Traian Dascălu

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Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture as revealed by STED nanoscopy by Waja Wegner, Heinz Steffens, Carola Gregor, Fred Wolf, Katrin I Willig

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Selecting FRET pairs for visualizing amyloid aggregation by Álvaro Ruiz-Arias, Rocío Jurado, Francisco Fueyo-González, Rosario Herranz, Natividad Gálvez, Juan A. González-Vera, Angel Orte

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Super-Resolution Microscopy Reveals an Altered Fibrin Network in Cirrhosis: The Key Role of Oxidative Stress in Fibrinogen Structural Modifications by Matteo Becatti, Amanda Mannucci, Flavia Rita Argento, Stefano Gitto, Francesco Vizzutti, Fabio Marra, Niccolò Taddei, Claudia Fiorillo, Giacomo Laffi

Subjects: “…stimulated emission depletion (STED) microscopy…”
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The Role of Lamins in the Nucleoplasmic Reticulum, a Pleiomorphic Organelle That Enhances Nucleo-Cytoplasmic Interplay by Merel Stiekema, Merel Stiekema, Frederik Houben, Frederik Houben, Fons Verheyen, Marcel Borgers, Julia Menzel, Martin Meschkat, Marc A. M. J. van Zandvoort, Marc A. M. J. van Zandvoort, Marc A. M. J. van Zandvoort, Marc A. M. J. van Zandvoort, Frans C. S. Ramaekers, Frans C. S. Ramaekers, Jos L. V. Broers, Jos L. V. Broers, Jos L. V. Broers

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A Bioorthogonally Applicable, Fluorogenic, Large Stokes-Shift Probe for Intracellular Super-Resolution Imaging of Proteins by Evelin Németh, Gergely Knorr, Krisztina Németh, Péter Kele

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Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini by Angelina Staudt, Olga Ratai, Aicha Bouzouina, Claudia Fecher-Trost, Ahmed Shaaban, Hawraa Bzeih, Alexander Horn, Ali H. Shaib, Ali H. Shaib, Margarete Klose, Veit Flockerzi, Marcel A. Lauterbach, Jens Rettig, Ute Becherer

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Improving two-photon excitation microscopy for sharper and faster biological imaging by Kohei Otomo, Hirokazu Ishii, Tomomi Nemoto

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Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse by Praneeth Chitirala, Hsin-Fang Chang, Paloma Martzloff, Christiane Harenberg, Keerthana Ravichandran, Midhat H Abdulreda, Per-Olof Berggren, Elmar Krause, Claudia Schirra, Trese Leinders-Zufall, Fritz Benseler, Nils Brose, Jens Rettig

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A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns by Marco Lindner, Marco Lindner, Aliz Tresztenyak, Gergö Fülöp, Wiebke Jahr, Adrian Prinz, Iris Prinz, Johann G. Danzl, Gerhard J. Schütz, Eva Sevcsik

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Genetic defects in β-spectrin and tau sensitize C. elegans axons to movement-induced damage via torque-tension coupling by Michael Krieg, Jan Stühmer, Juan G Cueva, Richard Fetter, Kerri Spilker, Daniel Cremers, Kang Shen, Alexander R Dunn, Miriam B Goodman

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Increased Mitochondrial Calcium Fluxes in Hypertrophic Right Ventricular Cardiomyocytes from a Rat Model of Pulmonary Artery Hypertension by Anna Maria Krstic, Amelia S. Power, Marie-Louise Ward

Subjects: “…stimulated emission depletion (STED) microscopy…”
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Fluorescence microscopy shadow imaging for neuroscience by V. V. G. Krishna Inavalli, Virginia Puente Muñoz, Virginia Puente Muñoz, Jonathan E. Draffin, Jonathan E. Draffin, Jan Tønnesen, Jan Tønnesen, Jan Tønnesen, Jan Tønnesen

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The Interaction of RecA With Both CheA and CheW Is Required for Chemotaxis by Elisabet Frutos-Grilo, Maria Marsal, Oihane Irazoki, Jordi Barbé, Susana Campoy

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