Stimulated emission depletion microscopy by Galiani, S, Koth, J, Schulze, U, Lagerholm, BC

“…Stimulated emission depletion (STED) microscopy is in its simplest form an extension of confocal fluorescence microscopy that offers much enhanced spatial resolution in both 2D and 3D. …”

Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes by Carravilla, P, Dasgupta, A, Zhurgenbayeva, G, Danylchuk, DI, Klymchenko, AS, Sezgin, E, Eggeling, C

“…Among the most popular super-resolution techniques, stimulated emission depletion (STED) microscopy offers one of the highest temporal resolutions, ultimately defined by the scanning speed. …”

Upgrade of a Scanning Confocal Microscope to a Single-Beam Path STED Microscope. by André Klauss, Marcelle König, Carsten Hille

“…By overcoming the diffraction limit in light microscopy, super-resolution techniques, such as stimulated emission depletion (STED) microscopy, are experiencing an increasing impact on life sciences. …”
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Role of the actin cytoskeleton in regulating LYVE-1 lateral diffusion and function by Stanly, T

“…At first, the native organisation of LYVE-1 and cross-linked clustered state of LYVE-1 (that are known to cause avidity induced increase in HA binding), on primary human dermal lymphatic endothelial cells (HDLECs), were identified by confocal and super-resolution STED microscopy. Following which the effects of actin disassembly on LYVE-1:HA binding function and cross-linked clustering of LYVE-1 were investigated using FACS and STED microscopy. …”

Auto-aligning stimulated emission depletion microscope using adaptive optics. by Gould, T, Kromann, E, Burke, D, Booth, M, Bewersdorf, J

“…Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. …”

The impact of signaling pathways on the desmosome ultrastructure in pemphigus by Thomas Schmitt, Julia Huber, Julia Pircher, Enno Schmidt, Enno Schmidt, Jens Waschke

“…The aim of this study was to identify the impact of several PV-related signaling pathways on desmosome ultrastructure.MethodsSTED microscopy, Dispase-based dissociation assay.ResultsAs observed using STED microscopy, pemphigus autoantibodies (PV-IgG) reduced desmosome number, decreased desmosome size, increased plaque distance and thickness and caused loss of adhesion. …”
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Analyzing the cellular plasma membrane by fast and efficient correlative STED and platinum replica EM by Dmytro Puchkov, Paul Markus Müller, Martin Lehmann, Claudia Matthaeus

“…Thus, implementation of super-resolution stimulated emission depletion (STED) microscopy offers significant reduction in sample preparation time and reduced technical challenges for imaging and analysis. …”
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Visualizing mitochondrial dynamics at the nanoscale by Till Stephan, Peter Ilgen, Stefan Jakobs

“…In this issue, Ren et al. introduce HBmito Crimson, a fluorogenic and photostable mitochondrial probe for STED microscopy and investigate how mitochondrial dynamics influence the spatial organization of mitochondrial DNA.…”
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Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-angstrom emitter localization. by Wildanger, D, Patton, B, Schill, H, Marseglia, L, Hadden, J, Knauer, S, Schönle, A, Rarity, J, O'Brien, J, Hell, S, Smith, J

“…Exploring the maximum spatial resolution achievable in far-field optical imaging, we show that applying solid immersion lenses (SIL) in stimulated emission depletion (STED) microscopy addresses single spins with a resolution down to 2.4 ± 0.3 nm and with a localization precision of 0.09 nm.…”

Sharper low-power STED nanoscopy by time gating. by Vicidomini, G, Moneron, G, Han, K, Westphal, V, Ta, H, Reuss, M, Engelhardt, J, Eggeling, C, Hell, S

“…Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. …”

Super-resolved traction force microscopy (STFM) by Colin-York, H, Shrestha, D, Felce, J, Waithe, D, Moeendarbary, E, Davis, S, Eggeling, C, Fritzsche, M

“…Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. …”

Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene by Qiqi Yang, Antonio Virgilio Failla, Petri Turunen, Ana Mateos-Maroto, Meiyu Gai, Werner Zuschratter, Sophia Westendorf, Márton Gelléri, Qiang Chen, Goudappagouda, Hao Zhao, Xingfu Zhu, Svenja Morsbach, Marcus Scheele, Wei Yan, Katharina Landfester, Ryota Kabe, Mischa Bonn, Akimitsu Narita, Xiaomin Liu

“…Abstract Stimulated emission depletion (STED) microscopy, a key optical super-resolution imaging method, has extended our ability to view details to resolution levels of tens of nanometers. …”
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Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes by Pablo Carravilla, Anindita Dasgupta, Gaukhar Zhurgenbayeva, Dmytro I. Danylchuk, Andrey S. Klymchenko, Erdinc Sezgin, Christian Eggeling

“…Among the most popular super-resolution techniques, stimulated emission depletion (STED) microscopy offers one of the highest temporal resolutions, ultimately defined by the scanning speed. …”
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Fast and Long‐Term Super‐Resolution Imaging of Endoplasmic Reticulum Nano‐structural Dynamics in Living Cells Using a Neural Network by Johanna V. Rahm, Ashwin Balakrishnan, Maren Wehrheim, Alexandra Kaminer, Marius Glogger, Laurell F. Kessler, Matthias Kaschube, Hans‐Dieter Barth, Mike Heilemann

“…Stimulated emission depletion (STED) microscopy is a super‐resolution technique that surpasses the diffraction limit and has contributed to the study of dynamic processes in living cells. …”
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z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics: supporting dataset by Barbotin, A, Urbančič, I, Galiani, S, Galiani, S, Eggeling, C, Booth, M, Sezgin, E

“…The compressed archive contains subfolders and instructions included in the relevant folders STED microscopy data of lipids in model systems and living cells.…”

Super-Resolved Traction Force Microscopy (STFM). by Colin-York, H, Shrestha, D, Felce, J, Waithe, D, Moeendarbary, E, Davis, S, Eggeling, C, Fritzsche, M

“…Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. …”

Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison. by Wegel, E, Göhler, A, Lagerholm, B, Wainman, A, Uphoff, S, Kaufmann, R, Dobbie, I

“…Super-resolution methods such as Structured Illumination Microscopy (SIM), STimulated Emission Depletion (STED) microscopy and Single Molecule Localisation Microscopy (SMLM) enable an increase in image resolution beyond the classical diffraction-limit. …”

Polarity-sensitive probes for superresolution stimulated emission depletion microscopy by Sezgin, E, Schneider, F, Zilles, V, Garcia, E, Waithe, D, Klymchenko, A, Eggeling, C

“…Here, we test the performance of the polarity sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA and NR12S in super resolution STED microscopy. Measurements on cell-derived membrane vesicles, in the plasma membrane of live cells, and on single virus particles show the high potential of these dyes for probing nanoscale membrane heterogeneity.…”

Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy by Antonello, J, Barbotin, A, Chong, EZ, Rittscher, J, Booth, MJ

“…To corroborate our arguments, we provide experimental verification of our method by performing aberration correction experiments in both confocal and STED microscopy using three different specimens.…”

Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light. by Han, K, Willig, K, Rittweger, E, Jelezko, F, Eggeling, C, Hell, S

“…Finally, we exemplify the potential of using nanodiamonds containing NV centers as luminescence tags in STED microscopy. Our results offer new experimental avenues in nanooptics, nanotechnology, and the life sciences.…”