Showing 101 - 120 results of 160 for search '"STED microscopy"', query time: 0.58s Refine Results
  1. 101

    Hyperosmotic stress: in situ chromatin phase separation by Ada L. Olins, Travis J. Gould, Logan Boyd, Bettina Sarg, Donald E. Olins

    Published 2020-01-01
    “…Fixed cells were imaged by immunostaining confocal and STED microscopy. At a “global” structural level (μm), mitotic chromosomes congeal into a residual gel with apparent (phase) separations of Ki67, CTCF, SMC2, RAD21, H1 histones and HMG proteins. …”
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  2. 102

    Condensin I protects meiotic cohesin from WAPL-1 mediated removal. by Margarita R Hernandez, Michael B Davis, Jianhao Jiang, Elizabeth A Brouhard, Aaron F Severson, Györgyi Csankovszki

    Published 2018-05-01
    “…Using conventional and stimulated emission depletion (STED) microscopy we show that levels of chromosomally-bound cohesin were significantly reduced in dpy-28 mutants, which lack a subunit of condensin I. …”
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  3. 103

    Tissue multicolor STED nanoscopy of presynaptic proteins in the calyx of Held. by Christian Kempf, Thorsten Staudt, Pit Bingen, Heinz Horstmann, Johann Engelhardt, Stefan W Hell, Thomas Kuner

    Published 2013-01-01
    “…While the latter have been observed with diffraction-limited fluorescence microscopy, STED microscopy for the first time revealed VGluT1-positive domains lacking synapsins. …”
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    Article
  4. 104

    High-resolution spatiotemporal analysis of single serotonergic axons in an in vitro system by Melissa Hingorani, Adele M. L. Viviani, Jenna E. Sanfilippo, Skirmantas Janušonis

    Published 2022-10-01
    “…This study examined single serotonergic axons in culture systems (co-cultures and monolayers), by using a set of complementary high-resolution methods: confocal microscopy, holotomography (refractive index-based live imaging), and super-resolution (STED) microscopy. It shows that serotonergic axon walks in neural tissue may strongly reflect the stochastic geometry of this tissue and it also provides new insights into the morphology and branching properties of serotonergic axons. …”
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    Article
  5. 105

    Real-time visualization of exo- and endocytosis membrane dynamics with confocal and super-resolution microscopy by Xiaoli Guo, Sue Han, Lisi Wei, Gianvito Arpino, Wonchul Shin, Xin Wang, Ling-Gang Wu

    Published 2022-06-01
    “…Here, we describe a protocol for imaging these membrane dynamics, including primary culture of bovine adrenal chromaffin cells, fluorescent probe application, patch-clamp to deliver depolarization and evoke exo- and endocytosis, electron microscopy (EM), and real-time confocal and stimulated emission depletion (STED) microscopy.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2016), Shin et al. (2018), and Shin et al. (2021). : Publisher's note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  6. 106

    Transient Fluorescence Labeling: Low Affinity—High Benefits by Maxim M. Perfilov, Alexey S. Gavrikov, Konstantin A. Lukyanov, Alexander S. Mishin

    Published 2021-10-01
    “…Notably, both localization microscopy and stimulated emission depletion (STED) microscopy impose tight restrictions on the physico-chemical properties of labels. …”
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  7. 107
  8. 108

    Investigating the active role of mechanical force during T-cell activation by Colin-York, H

    Published 2016
    “…With the aim of measuring mechanical force generated at the IS, we present a novel method combining the super resolution imaging technique, Stimulated Emission Depletion (STED) microscopy and Traction Force Microscopy (TFM). …”
    Thesis
  9. 109

    Sensorless adaptive optics for isoSTED nanoscopy by Antonello, J, Hao, X, Allgeyer, E, Bewersdorf, J, Rittscher, J, Booth, M

    Published 2018
    “…This simplistic approach has limited applicability to isoSTED microscopy where, due to the complex interplay between the excitation and depletion foci, maximising the total image brightness can lead to introducing aberrations in the depletion foci. …”
    Conference item
  10. 110

    Guanylate-binding protein 1 acts as a pro-viral factor for the life cycle of hepatitis C virus. by Daniela Bender, Alexandra Koulouri, Xingjian Wen, Mirco Glitscher, Anja Schollmeier, Liliana Fernandes da Costa, Robin Oliver Murra, Gert Paul Carra, Vanessa Haberger, Gerrit J K Praefcke, Eberhard Hildt

    Published 2024-02-01
    “…To monitor the expression and subcellular distribution of GBP1 and HCV we performed qPCR, Western blot, CLSM and STED microscopy, virus titration and reporter gene assays. …”
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    Article
  11. 111

    Analysis of replication factories in human cells by super-resolution light microscopy by Schwarz Ulf, Cseresnyes Zoltan, Green Catherine M

    Published 2009-12-01
    “…We have used stimulated emission depletion (STED) microscopy, which improves on the resolving power of the confocal microscope, to probe the structure of these factories at sub-diffraction limit resolution.…”
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  12. 112

    CDT of <i>Clostridioides difficile</i> Induces MLC-Dependent Intestinal Barrier Dysfunction in HT-29/B6 Epithelial Cell Monolayers by Lucas Heils, Martina Schneemann, Ralf Gerhard, Jörg-Dieter Schulzke, Roland Bücker

    Published 2023-01-01
    “…Molecular analysis of the spatial distribution of tight junction protein and cytoskeleton was performed by super-resolution STED microscopy. Results: Sublethal concentrations of CDT-induced barrier dysfunction with decreased TER and increased permeability for 332 Da fluorescein and 4 kDa FITC-dextran. …”
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  13. 113

    Lipid composition but not curvature is the determinant factor for the low molecular mobility observed on the membrane of virus-like vesicles by Urbančič, I, Brun, J, Shrestha, D, Waithe, D, Eggeling, C, Chojnacki, J

    Published 2018
    “…To further refine the model of the molecular mobility on the HIV-1 surface, we herein investigated the relative importance of membrane composition and curvature in simplified model membrane systems, large unilamellar vesicles (LUVs) of different lipid compositions and sizes (0.1–1 µm), using super-resolution stimulated emission depletion (STED) microscopy-based fluorescence correlation spectroscopy (STED-FCS). …”
    Journal article
  14. 114

    Plasma membrane dynamics in immune signalling by Schneider, F

    Published 2019
    “…A combination of super-resolution stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy (FCS) is employed to obtain dynamic information at the nano-scale and developed further to measure the diffusion behaviour of lipids and proteins in living cells and model membrane systems across space at sub-diffraction resolution. …”
    Thesis
  15. 115

    Optimized protocol for live-cell analysis of kinetochore fiber maturation in Indian muntjac cells by Ana Coelho Almeida, Joana Soares-de-Oliveira, Helder Maiato

    Published 2023-03-01
    “…Specifically, we detail the combination of live-cell super-resolution CH-STED microscopy of microtubule growth events within individual k-fibers and a laser-mediated k-fiber injury/repair assay. …”
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  16. 116

    High-Resolution Microscopic Characterization of Tunneling Nanotubes in Living U87 MG and LN229 Glioblastoma Cells by Nicole Matejka, Asieh Amarlou, Jessica Neubauer, Sarah Rudigkeit, Judith Reindl

    Published 2024-03-01
    “…In this study, we investigate the properties of TNTs in two glioblastoma cell lines, U87 MG and LN229, including measurements of their diameter by high-resolution live-cell stimulated emission depletion (STED) microscopy and an analysis of their length, morphology, lifetime, and formation by live-cell confocal microscopy. …”
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  17. 117

    RIM-BP2 regulates Ca2+ channel abundance and neurotransmitter release at hippocampal mossy fiber terminals by Rinako Miyano, Hirokazu Sakamoto, Kenzo Hirose, Takeshi Sakaba

    Published 2024-02-01
    “…Consistent with presynaptic recording, STED microscopy suggested less abundance of P/Q-type Ca2+ channels at AZs deficient in RIM-BP2. …”
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  18. 118

    Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers by Valentina Štimac, Isabella Koprivec, Martina Manenica, Juraj Simunić, Iva M Tolić

    Published 2022-10-01
    “…Whereas augmin-nucleated bridging fibers prevent merotelic attachments by creating a nearly parallel and highly bundled microtubule arrangement unfavorable for creating additional attachments, augmin-nucleated k-fibers produce robust force required to resolve errors during anaphase. STED microscopy revealed that bridging fibers were impaired twice as much as k-fibers following augmin depletion. …”
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  19. 119

    The Pseudomonas aeruginosa N-acylhomoserine lactone quorum sensing molecules target IQGAP1 and modulate epithelial cell migration. by Thommie Karlsson, Maria V Turkina, Olena Yakymenko, Karl-Eric Magnusson, Elena Vikström

    Published 2012-01-01
    “…Moreover, 3O-C(12)-HSL induced changes in the phosphorylation status of Rac1 and Cdc42 and the localization of IQGAP1 as evidenced by confocal and STED microscopy and Western blots. Our findings suggest that the IQGAP1 is a novel partner for P. aeruginosa 3O-C(12)-HSL and likely the integrator of Rac1 and Cdc42- dependent altered cell migration. …”
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  20. 120

    Comparing Super-Resolution Microscopy Techniques to Analyze Chromosomes by Ivona Kubalová, Alžběta Němečková, Klaus Weisshart, Eva Hřibová, Veit Schubert

    Published 2021-02-01
    “…Instead, super-resolution by stimulated emission depletion (STED) microscopy achieving a resolution of ~50 nm laterally and ~130 nm axially has not yet frequently been applied in plant cell research due to the required specific sample preparation and stable dye staining. …”
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