Wavefront shaping for stimulated emission depletion microscopy by Barbotin, A

“…Several super-resolution microscopy techniques exist, among which stimulated emission depletion (STED) microscopy has become a tool of choice for many biological applications, particularly in living specimens. …”

Analysis of replication factories in human cells by super-resolution light microscopy by Cseresnyes, Z, Schwarz, U, Green, C

“…We have used stimulated emission depletion (STED) microscopy, which improves on the resolving power of the confocal microscope, to probe the structure of these factories at sub-diffraction limit resolution. …”

Smart Nanoscopy: A Review of Computational Approaches to Achieve Super-Resolved Optical Microscopy by Shiraz S. Kaderuppan, Eugene Wai Leong Wong, Anurag Sharma, Wai Lok Woo

“…Numerous optical implementations allowing for a new frontier in traditional confocal laser scanning fluorescence microscopy to be explored (termed super-resolution fluorescence microscopy) have been realized through the development of techniques such as stimulated emission and depletion (STED) microscopy, photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), amongst others. …”
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Lymphocytic choriomeningitis arenavirus utilises intercellular connections for cell to cell spread by Owen Byford, Amelia B. Shaw, Hiu Nam Tse, Alex Moon-Walker, Erica Ollmann Saphire, Sean P. J. Whelan, Martin Stacey, Roger Hewson, Juan Fontana, John N. Barr

“…Within these TNT-like connections, confocal and STED microscopy identified puncta containing the major structural components of LCMV virions alongside genomic RNA, consistent with intercellular transit of assembled virions or ribonucleoprotein genome segments. …”
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On the Adjacency Matrix of RyR2 Cluster Structures. by Mark A Walker, Tobias Kohl, Stephan E Lehnart, Joseph L Greenstein, W J Lederer, Raimond L Winslow

“…We recapitulate these results by applying the model to realistic RyR2 cluster structures informed by super-resolution stimulated emission depletion (STED) microscopy. Eigendecomposition of the linearized mean-field contact network model reveals functional subdomains within RyR2 clusters with distinct sensitivities to Ca2+. …”
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Confocal and Super-resolution Imaging of RNA in Live Bacteria Using a Fluorogenic Silicon Rhodamine-binding Aptamer by Regina Wirth, Peng Gao, G. Nienhaus, Murat Sunbul, Andres Jäschke

“…Notably, with SiRA, we demonstrated the first aptamer-based RNA visualization using super-resolution (STED) microscopy. This imaging method can be especially valuable for visualization of RNA in prokaryotes since the size of a bacterium is only a few times greater than the optical resolution of a conventional microscope.…”
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Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation by Mariano Gonzalez Pisfil, Iliya Nadelson, Brigitte Bergner, Sonja Rottmeier, Andreas W. Thomae, Steffen Dietzel

“…Abstract Stimulated emission depletion (STED) microscopy achieves super-resolution by exciting a diffraction-limited volume and then suppressing fluorescence in its outer parts by depletion. …”
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Terminal axonal arborization and synaptic bouton formation critically rely on abp1 and the arp2/3 complex. by Nicole Koch, Oliver Kobler, Ulrich Thomas, Britta Qualmann, Michael M Kessels

“…We show that the F-actin-binding protein Abp1 is prominently expressed in the Drosophila nervous system and reveal that Abp1 is an important regulator in shaping glutamatergic neuromuscular junctions (NMJs) of flies. STED microscopy shows that Abp1 accumulations can be found in close proximity of synaptic vesicles and at the cell cortex in nerve terminals. …”
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A modular framework for multi-scale tissue imaging and neuronal segmentation by Simone Cauzzo, Ester Bruno, David Boulet, Paul Nazac, Miriam Basile, Alejandro Luis Callara, Federico Tozzi, Arti Ahluwalia, Chiara Magliaro, Lydia Danglot, Nicola Vanello

“…Specifically, we set up a method for labeling thick samples and develop SENPAI, a scalable algorithm for segmenting neurons at cellular and sub-cellular scales in conventional and super-resolution STimulated Emission Depletion (STED) microscopy images of brain tissues. Further, we propose a validation paradigm for testing segmentation performance when a manual ground-truth may not exhaustively describe neuronal arborization. …”
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Specific Interaction of DARPin with HIV-1 CA_NTD Disturbs the Distribution of Gag, RNA Packaging, and Tetraspanin Remodelling in the Membrane by Sutpirat Moonmuang, Rawiwan Maniratanachote, Paninee Chetprayoon, Kanokporn Sornsuwan, Weeraya Thongkum, Koollawat Chupradit, Chatchai Tayapiwatana

“…By applying stimulated emission-depletion (STED) microscopy, Gag polymerisation was interrupted at the plasma membrane. …”
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SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole by Elisabetta Di Franco, Angelita Costantino, Elena Cerutti, Morgana D’Amico, Anna P. Privitera, Paolo Bianchini, Giuseppe Vicidomini, Massimo Gulisano, Alberto Diaspro, Luca Lanzanò

“…We demonstrate applicability on different types of imaging modalities: (1) confocal imaging of DNA in a non-adherent cell line; (2) removal of out-of-focus background in super-resolved STED microscopy; (3) imaging of live intestinal organoids stained with a membrane dye.…”
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An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells by Samantha E Wilner, Brian Wengerter, Keith Maier, Maria de Lourdes Borba Magalhães, David Soriano Del Amo, Supriya Pai, Felipe Opazo, Silvio O Rizzoli, Amy Yan, Matthew Levy

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Unc13A dynamically stabilizes vesicle priming at synaptic release sites for short-term facilitation and homeostatic potentiation by Meida Jusyte, Natalie Blaum, Mathias A. Böhme, Manon M.M. Berns, Alix E. Bonard, Ábel B. Vámosi, Kavya V. Pushpalatha, Janus R.L. Kobbersmed, Alexander M. Walter

“…Labeling the functionally essential Unc13A MUN domain reveals higher STED microscopy signals closer to release sites following CaM-domain mutation. …”
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Injury to Cone Synapses by Retinal Detachment: Differences from Rod Synapses and Protection by ROCK Inhibition by Ellen Townes-Anderson, Éva Halász, Ilene Sugino, Amy L. Davidow, Laura J. Frishman, Luke Fritzky, Fawad A. K. Yousufzai, Marco Zarbin

“…Conventional confocal and stimulated emission depletion (STED) microscopy were used for morphological assessment and electroretinograms for functional analysis of an adult pig model of RD. …”
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Aberrations in Structured Illumination Microscopy: A Theoretical Analysis by Xin Liu, Shijie Tu, Yan Xu, Hongya Song, Wenjie Liu, Qiulan Liu, Cuifang Kuang, Cuifang Kuang, Cuifang Kuang, Xu Liu, Xu Liu, Xu Liu, Xiang Hao

“…In previous works, the aberrations in stimulated emission depletion (STED) microscopy and single-molecule localization microscopy (SMLM) have been well-investigated, while the research on the aberrations in structured illumination microscopy (SIM) is not sufficient, the researchers always poured attention into aberrations only in the detection path. …”
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Polarized α-synuclein trafficking and transcytosis across brain endothelial cells via Rab7-decorated carriers by Parvez Alam, Mikkel R. Holst, Line Lauritsen, Janni Nielsen, Simone S. E. Nielsen, Poul Henning Jensen, Jonathan R. Brewer, Daniel E. Otzen, Morten S. Nielsen

“…Here, we observed the highest colocalization with clathrin, Rab7 and VPS35, suggesting a clathrin-dependent internalization, preferentially followed by a late endosome retromer-connected trafficking pathway. Furthermore, STED microscopy revealed monomeric α-syn trafficking via Rab7-decorated carriers. …”
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Inheritance of the reduced mitochondria of Giardia intestinalis is coupled to the flagellar maturation cycle by Pavla Tůmová, Luboš Voleman, Andreas Klingl, Eva Nohýnková, Gerhard Wanner, Pavel Doležal

“…Results We combined 2D stimulated emission depletion (STED) microscopy and focused ion beam scanning electron microscopy (FIB/SEM) to show that mitosomes exhibit internal segmentation and conserved asymmetric structure. …”
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Adrenergic Signaling-Induced Ultrastructural Strengthening of Intercalated Discs via Plakoglobin Is Crucial for Positive Adhesiotropy in Murine Cardiomyocytes by Sunil Yeruva, Ellen Kempf, Desalegn Tadesse Egu, Heinrich Flaswinkel, Daniela Kugelmann, Jens Waschke

“…Quantitative transmission electron microscopy (TEM) analysis of ICD demonstrated that cAMP elevation caused significant elongation of area composita and thickening of the ICD plaque, paralleled by enhanced cardiomyocyte cohesion, in WT but not PG-deficient cardiomyocytes. STED microscopy analysis supported that cAMP elevation ex vivo enhanced overlap of desmoglein-2 (Dsg2) and N-cadherin (N-cad) staining in ICDs of WT but not PG-deficient cardiomyocytes. …”
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A single-domain antibody detects and neutralises toxic Aβ42 oligomers in the Alzheimer’s disease CSF by Alessandra Bigi, Liliana Napolitano, Devkee M. Vadukul, Fabrizio Chiti, Cristina Cecchi, Francesco A. Aprile, Roberta Cascella

“…Methods We investigated the ability of DesAb-O to selectively detect preformed Aβ42 oligomers both in vitro and in cultured neuronal cells, by using dot-blot, ELISA immunoassay and super-resolution STED microscopy, and to counteract the toxicity induced by the oligomers, monitoring their interaction with neuronal membrane and the resulting mitochondrial impairment. …”
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Synthesis and biological characterization of natural and designed sugars by Kiappes, J

“…In order to fully characterize the mechanism, the mentioned enzymatic and antiviral studies were supplemented with lipidomic, STED-microscopy and pharmacokinetic investigations. </p>…”