Efficient acquisition of tens of thousands of short tandem repeats in single-cell whole-genome-amplified DNA by Liming Tao, Zipora Marx, Ofir Raz, Ehud Shapiro

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Droplet-based microfluidic analysis and screening of single plant cells. by Ziyi Yu, Christian R Boehm, Julian M Hibberd, Chris Abell, Jim Haseloff, Steven J Burgess, Ivan Reyna-Llorens

“…This work opens the door to droplet-based microfluidic analysis of plant cells for applications ranging from high-throughput characterisation of DNA parts to single-cell genomics to selection of rare plant phenotypes.…”
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Exploring the Complexity of Cortical Development Using Single-Cell Transcriptomics by Hyobin Jeong, Vijay K. Tiwari

“…We also discuss how single-cell genomics holds a big potential in future for brain research and discuss its possible applications and biological insights that can be achieved from these approaches. …”
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Hepatic Stellate Cell Regulation of Liver Regeneration and Repair by Laura J. Kitto, Neil C. Henderson

“…As our knowledge of the cellular and molecular mechanisms driving hepatic regeneration deepens, the key role of the mesenchymal compartment during the regenerative response has been increasingly appreciated. Single‐cell genomics approaches have recently uncovered both spatial and functional zonation of the hepatic mesenchyme in homeostasis and following liver injury. …”
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Challenges and Opportunities for the Translation of Single-Cell RNA Sequencing Technologies to Dermatology by Alex M. Ascensión, Marcos J. Araúzo-Bravo, Ander Izeta

“…This complexity is beginning to be understood through the application of single-cell genomics and computational tools. A large number of datasets that shed light on how the different human skin cell types interact in homeostasis—and what ceases to work in diverse dermatological diseases—have been generated and are publicly available. …”
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Microbial Metazoa Are Microbes Too by Holly M. Bik

“…The majority of species are too large to be considered in single-cell genomics approaches, yet too small to be wrapped into international genome sequencing initiatives. …”
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Single cell analysis of normal and leukemic hematopoiesis by Povinelli, B, Rodriguez-Meira, A, Mead, A

“…In this review, we summarize how single cell techniques have been applied to the analysis of hematopoietic stem/progenitor cells in normal and malignant hematopoiesis, with a particular focus on recent advances in single-cell genomics, including how these might be utilized for clinical application.…”

Uncovering pseudotemporal trajectories with covariates from single cell and bulk expression data by Campbell, K, Yau, C

“…This hybrid regression-latent variable model framework extends pseudotemporal modelling from its most prevalent area of single cell genomics to wider applications.…”

COVID-19 relapse associated with SARS-CoV-2 evasion from CD4+ T-cell recognition in an agammaglobulinemia patient by Ryo Morita, Ritsuko Kubota-Koketsu, Xiuyuan Lu, Tadahiro Sasaki, Emi E. Nakayama, Yu-chen Liu, Daisuke Okuzaki, Daisuke Motooka, James Badger Wing, Yasunori Fujikawa, Yuji Ichida, Kiyoko Amo, Tetsushi Goto, Junichi Hara, Michinori Shirano, Sho Yamasaki, Tatsuo Shioda

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scATAC-pro: a comprehensive workbench for single-cell chromatin accessibility sequencing data by Wenbao Yu, Yasin Uzun, Qin Zhu, Changya Chen, Kai Tan

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Transcriptional profiling of mouse projection neurons with VECTORseq by Victoria Cheung, Philip Chung, Evan H. Feinberg

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MDSC in Mice and Men: Mechanisms of Immunosuppression in Cancer by Christophe Vanhaver, Pierre van der Bruggen, Annika M. Bruger

“…In future, bulk or single-cell genomics could elucidate which phenotypic and functional phenotypes MDSCs adopt in particular microenvironments and help to identify potential targets for therapy.…”
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Reconstructing each cell’s genome within complex microbial communities - dream or reality? by Scott eClingenpeel, Alicia eClum, Patrick eSchwientek, Christian eRinke, Tanja eWoyke

“…These methods, namely metagenomics and more recently single-cell genomics, have become cornerstones for microbial ecology and environmental microbiology. …”
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Single-cell sequencing of the small and AT-skewed genome of malaria parasites by Shiwei Liu, Adam C. Huckaby, Audrey C. Brown, Christopher C. Moore, Ian Burbulis, Michael J. McConnell, Jennifer L. Güler

“…Abstract Single-cell genomics is a rapidly advancing field; however, most techniques are designed for mammalian cells. …”
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A simple strategy for reducing false negatives in calling variants from single-cell sequencing data. by Cong Ji, Zong Miao, Xionglei He

“…Due to the growth of interest in single-cell genomics, computational methods for distinguishing true variants from artifacts are highly desirable. …”
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Single-cell technologies for multimodal omics measurements by Dongsheng Bai, Chenxu Zhu, Chenxu Zhu

“…The recent surge in single-cell genomics, including the development of a wide range of experimental and computational approaches, has provided insights into the complex molecular networks of cells during development and in human diseases at unprecedented resolution. …”
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Nuclear Mechanopathology and Cancer Diagnosis by Uhler, Caroline, Shivashankar, G.V.

“…Finally, we highlight the importance of digital pathology based on nuclear morphometric features combined with single-cell genomics for early cancer diagnostics.…”
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Illuminating novel biological aspects and potential new therapeutic approaches for chronic myeloproliferative malignancies by Mughal, TI, Pemmaraju, N, Psaila, B, Radich, J, Bose, P, Lion, T, Kiladjian, J-J, Rampal, R, Jain, T, Verstovsek, S, Yacoub, A, Cortes, JE, Mesa, R, Saglio, G, van Etten, RA

“…We consider how recent studies using single-cell genomics and other molecular methods reveal novel aspects of hematopoiesis which in turn raise the possibility of new therapeutic approaches for patients with myeloproliferative neoplasms (MPNs). …”

Community-like genome in single cells of the sulfur bacterium Achromatium oxaliferum by Ionescu, D, Bizic-Ionescu, M, De Maio, N, Cypionka, H, Grossart, H

“…Polyploid bacteria are common, but the genetic and functional diversity resulting from polyploidy is unknown. Here we use single-cell genomics, metagenomics, single-cell amplicon sequencing, and fluorescence in situ hybridization, to show that individual cells of Achromatium oxaliferum, the world's biggest known freshwater bacterium, harbor genetic diversity typical of whole bacterial communities. …”

Identification of drug candidates targeting monocyte reprogramming in people living with HIV by Rainer Knoll, Rainer Knoll, Lorenzo Bonaguro, Lorenzo Bonaguro, Jéssica C. dos Santos, Jéssica C. dos Santos, Stefanie Warnat-Herresthal, Stefanie Warnat-Herresthal, Maartje C. P. Jacobs-Cleophas, Maartje C. P. Jacobs-Cleophas, Edda Blümel, Nico Reusch, Arik Horne, Arik Horne, Miriam Herbert, Miriam Herbert, Melanie Nuesch-Germano, Twan Otten, Twan Otten, Wouter A. van der Heijden, Wouter A. van der Heijden, Lisa van de Wijer, Lisa van de Wijer, Alex K. Shalek, Alex K. Shalek, Alex K. Shalek, Kristian Händler, Kristian Händler, Matthias Becker, Marc D. Beyer, Marc D. Beyer, Mihai G. Netea, Mihai G. Netea, Mihai G. Netea, Leo A. B. Joosten, Leo A. B. Joosten, Leo A. B. Joosten, Andre J. A. M. van der Ven, Andre J. A. M. van der Ven, Joachim L. Schultze, Joachim L. Schultze, Joachim L. Schultze, Anna C. Aschenbrenner

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