SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells by Joshua Jackson, Lena Wischhof, Enzo Scifo, Anna Pellizzer, Yiru Wang, Antonia Piazzesi, Debora Gentile, Sana Siddig, Miriam Stork, Chris E. Hopkins, Kristian Händler, Joachim Weis, Andreas Roos, Joachim L. Schultze, Pierluigi Nicotera, Dan Ehninger, Daniele Bano

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Applying Single-Cell Analysis to Gonadogenesis and DSDs (Disorders/Differences of Sex Development) by Martin A. Estermann, Craig A. Smith

“…The emergence of single-cell genomics and transcriptomics now presents a novel avenue for DSD analysis, for both diagnosis and for understanding the molecular genetic etiology. …”
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A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors

“…© 2020, The Author(s). Single-cell genomics is essential to chart tumor ecosystems. …”
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Programming human cell fate: overcoming challenges and unlocking potential through technological breakthroughs by Lin, H-C, Makhlouf, A, Vazquez Echegaray, C, Zawada, D, Simoes, F

“…The Company of Biologists’ 2023 workshop on ‘Novel Technologies for Programming Human Cell Fate’ brought together experts in human cell fate engineering and experts in single-cell genomics, manipulation and characterisation of cells on a single (sub)cellular level. …”

Identification of the novel FOXP3-dependent Treg cell transcription factor MEOX1 by high-dimensional analysis of human CD4+ T cells by Kevin Baßler, Kevin Baßler, Lisa Schmidleithner, Mehrnoush Hadaddzadeh Shakiba, Tarek Elmzzahi, Tarek Elmzzahi, Maren Köhne, Stefan Floess, Rebekka Scholz, Naganari Ohkura, Timothy Sadlon, Kathrin Klee, Anna Neubauer, Shimon Sakaguchi, Simon C. Barry, Jochen Huehn, Lorenzo Bonaguro, Lorenzo Bonaguro, Thomas Ulas, Thomas Ulas, Thomas Ulas, Marc Beyer, Marc Beyer

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Significance of interferon signaling based on mRNA-microRNA integration and plasma protein analyses in critically ill COVID-19 patients by Yuki Togami, Hisatake Matsumoto, Jumpei Yoshimura, Tsunehiro Matsubara, Takeshi Ebihara, Hiroshi Matsuura, Yumi Mitsuyama, Takashi Kojima, Masakazu Ishikawa, Fuminori Sugihara, Haruhiko Hirata, Daisuke Okuzaki, Hiroshi Ogura

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Multi-omics segregate different transcriptomic impacts of anti-IL-17A blockade on type 17 T-cells and regulatory immune cells in psoriasis skin by Jaehwan Kim, Jaehwan Kim, Jaehwan Kim, Jongmi Lee, Xuan Li, Norma Kunjravia, Darshna Rambhia, Inna Cueto, Katherine Kim, Katherine Kim, Vasuma Chaparala, Younhee Ko, Younhee Ko, Sandra Garcet, Sandra Garcet, Wei Zhou, Junyue Cao, James G. Krueger

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Highly multiplexed single-cell quantitative PCR. by Michael VanInsberghe, Hans Zahn, Adam K White, Oleh I Petriv, Carl L Hansen

“…Highly multiplexed microfluidic RT-qPCR fills a gap in current capabilities for single-cell analysis, providing a rapid and cost-effective approach for profiling panels of marker genes, thereby complementing single-cell genomics methods that are best suited for global analysis and discovery. …”
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A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications by Ashraful Haque, Jessica Engel, Sarah A. Teichmann, Tapio Lönnberg

“…Since the first single-cell RNA-sequencing (scRNA-seq) study was published in 2009, many more have been conducted, mostly by specialist laboratories with unique skills in wet-lab single-cell genomics, bioinformatics, and computation. However, with the increasing commercial availability of scRNA-seq platforms, and the rapid ongoing maturation of bioinformatics approaches, a point has been reached where any biomedical researcher or clinician can use scRNA-seq to make exciting discoveries. …”
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Editorial: Monocyte Heterogeneity and Function by Florent Ginhoux, Florent Ginhoux, Florent Ginhoux, Alexander Mildner, Emmanuel L. Gautier, Andreas Schlitzer, Andreas Schlitzer, Claudia Jakubzick, Chen Varol, Calum Bain, Pierre Guermonprez, Pierre Guermonprez

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Diverse Marinimicrobia bacteria may mediate coupled biogeochemical cycles along eco-thermodynamic gradients by Alyse K. Hawley, Masaru K. Nobu, Jody J. Wright, W. Evan Durno, Connor Morgan-Lang, Brent Sage, Patrick Schwientek, Brandon K. Swan, Christian Rinke, Monica Torres-Beltrán, Keith Mewis, Wen-Tso Liu, Ramunas Stepanauskas, Tanja Woyke, Steven J. Hallam

“…Here, the authors study global populations of Marinimicrobia using single-cell genomics, metagenomics and metatranscriptomics, showing potential co-metabolic interactions and participation in the sulfur and nitrogen cycles.…”
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Do Ruminal Ciliates Select Their Preys and Prokaryotic Symbionts? by Tansol Park, Zhongtang Yu

“…The bacteria enriched or exclusively found in the CAP can be target bacteria to detect and localize using specific probes designed from their 16S rRNA sequences, to characterize using single-cell genomics, or to isolate using new media designed based on genomic information.…”
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scGR-seq: Integrated analysis of glycan and RNA in single cells by Haruki Odaka, Haruka Ozaki, Hiroaki Tateno

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Discovering Cellular Mitochondrial Heteroplasmy Heterogeneity with Single Cell RNA and ATAC Sequencing by Aidan S. Marshall, Nick S. Jones

“…Its application to single-cell genomics has generated a dynamic and evolving field with a vast amount of research highlighting heterogeneity in transcriptional, genetic and epigenomic state between cells. …”
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Monodisperse Picoliter Droplets for Low-Bias and Contamination-Free Reactions in Single-Cell Whole Genome Amplification. by Yohei Nishikawa, Masahito Hosokawa, Toru Maruyama, Keisuke Yamagishi, Tetsushi Mori, Haruko Takeyama

“…Our results demonstrate that microfluidic-generated droplets show potential as an efficient tool for effective amplification of low-input DNA for single-cell genomics and greatly reduce the cost and labor investment required for determination of nearly complete genome sequences of uncultured bacteria from environmental samples.…”
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Risk-associated alterations in marrow T cells in pediatric leukemia by Jithendra Kini Bailur, Samuel S. McCachren, Katherine Pendleton, Juan C. Vasquez, Hong Seo Lim, Alyssa Duffy, Deon B. Doxie, Akhilesh Kaushal, Connor Foster, Deborah DeRyckere, Sharon Castellino, Melissa L. Kemp, Peng Qiu, Madhav V. Dhodapkar, Kavita M. Dhodapkar

“…Here, we combine mass cytometry, single cell genomics, and functional studies to characterize the BM immune environment in children with B cell acute lymphoblastic leukemia and acute myelogenous leukemia at presentation. …”
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Plant Single-Cell Metabolomics—Challenges and Perspectives by Leonardo Perez de Souza, Monica Borghi, Alisdair Fernie

“…In this context, single-cell genomics and transcriptomics quickly became a well-established tool to answer fundamental questions challenging to assess at a whole tissue level. …”
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In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations by Fatemeh Safi, Parashar Dhapola, Eva Erlandsson, Linda Geironson Ulfsson, Ariana S. Calderón, Charlotta Böiers, Göran Karlsson

“…This protocol represents an efficient tool for validation experiments for single-cell genomics data.For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Single-cell metabolic profiling reveals subgroups of primary human hepatocytes with heterogeneous responses to drug challenge by Eva Sanchez-Quant, Maria Lucia Richter, Maria Colomé-Tatché, Celia Pilar Martinez-Jimenez

“…Recent advances in single-cell genomics demonstrate liver zonation and ploidy as main drivers of cellular heterogeneity. …”
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Genomic and molecular control of cell type and cell type conversions by Xiuling Fu, Fangfang He, Yuhao Li, Allahverdi Shahveranov, Andrew Paul Hutchins

“…The onset of single cell technologies, and more recently genomics (particularly single cell genomics), has substantially increased the understanding of the concept of cell type, but has also increased the complexity of this understanding. …”
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