Whole-Genome Amplification—Surveying Yield, Reproducibility, and Heterozygous Balance, Reported by STR-Targeting MIPs by Ofir Raz, Liming Tao, Tamir Biezuner, Tzipy Marx, Yaara Neumeier, Narek Tumanyan, Ehud Shapiro

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Computational Methods for Single-cell DNA Methylome Analysis by Waleed Iqbal, Wanding Zhou

Subjects: “…Single-cell genomics…”
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The Human Cell Atlas: making ‘cell space’ for disease by Chris P. Ponting

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Revealing within-species diversity in uncultured human gut bacteria with single-cell long-read sequencing by Masato Kogawa, Yohei Nishikawa, Yohei Nishikawa, Tatsuya Saeki, Takuya Yoda, Koji Arikawa, Haruko Takeyama, Haruko Takeyama, Haruko Takeyama, Haruko Takeyama, Masahito Hosokawa, Masahito Hosokawa, Masahito Hosokawa, Masahito Hosokawa, Masahito Hosokawa

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Improved environmental genomes via integration of metagenomic and single-cell assemblies by Daniel Richard Mende, Frank O'Neill Aylward, John M Eppley, Torben N Nielsen, Edward F. DeLong

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Targeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral by Keigo Ide, Yohei Nishikawa, Toru Maruyama, Yuko Tsukada, Masato Kogawa, Hiroki Takeda, Haruka Ito, Ryota Wagatsuma, Rimi Miyaoka, Yoshikatsu Nakano, Koji Kinjo, Michihiro Ito, Masahito Hosokawa, Kei Yura, Shoichiro Suda, Haruko Takeyama

“…Results In this study, we applied a novel single-cell genomics technique using droplet microfluidics to obtain single-cell amplified genomes (SAGs) for uncultured coral-associated Endozoicomonas spp. …”
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Chromosomal Instability Estimation Based on Next Generation Sequencing and Single Cell Genome Wide Copy Number Variation Analysis. by Stephanie B Greene, Angel E Dago, Laura J Leitz, Yipeng Wang, Jerry Lee, Shannon L Werner, Steven Gendreau, Premal Patel, Shidong Jia, Liangxuan Zhang, Eric K Tucker, Michael Malchiodi, Ryon P Graf, Ryan Dittamore, Dena Marrinucci, Mark Landers

“…For proof of concept, prostate cancer cell lines, LNCaP, PC3 and VCaP, were spiked into healthy donor blood to create mock patient-like samples for downstream single cell genomic analysis. In addition, samples from seven metastatic castration resistant prostate cancer (mCRPC) patients were included to evaluate clinical feasibility. …”
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Oncogenes and cancer associated thrombosis: what can we learn from single cell genomics about risks and mechanisms? by Nadim Tawil, Abdulshakour Mohammadnia, Janusz Rak

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Genomic Characteristics and Potential Metabolic Adaptations of Hadal Trench Roseobacter and Alteromonas Bacteria Based on Single-Cell Genomics Analyses by Mingming Chen, Yu Song, Xiaoyuan Feng, Kai Tang, Nianzhi Jiao, Jiwei Tian, Yao Zhang

Subjects: “…single-cell genome…”
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Correction to: scBFA: modeling detection patterns to mitigate technical noise in large-scale single-cell genomics data by Ruoxin Li, Gerald Quon

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A single-cell genomic based investigation of immune cells in the aorta and adipose tissue during metabolic disease progression by Zurke, YX

Phylogeography, salinity adaptations and metabolic potential of the Candidate Division KB1 Bacteria based on a partial single cell genome. by Lisa M Nigro, Andrew S Hyde, Barbara J. MacGregor, Andreas Teske

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Deciphering cell–cell interactions and communication in the tumor microenvironment and unraveling intratumoral genetic heterogeneity via single-cell genomic sequencing by Ya-Hong Cao, Jie Ding, Qing-Hai Tang, Jie Zhang, Zhong-Yan Huang, Xiao-Mei Tang, Ji-Bin Liu, Yu-Shui Ma, Da Fu

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Single Cell Genomics-Based Analysis of Gene Content and Expression of Prophages in a Diffuse-Flow Deep-Sea Hydrothermal System by Jessica M. Labonté, Jessica M. Labonté, Maria Pachiadaki, Maria Pachiadaki, Elizabeth Fergusson, Jesse McNichol, Ashley Grosche, Lara K. Gulmann, Costantino Vetriani, Costantino Vetriani, Stefan M. Sievert, Ramunas Stepanauskas

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Single-Cell Genomics Reveals a Diverse Metabolic Potential of Uncultivated Desulfatiglans-Related Deltaproteobacteria Widely Distributed in Marine Sediment by Lara M. Jochum, Lars Schreiber, Ian P. G. Marshall, Bo B. Jørgensen, Andreas Schramm, Kasper U. Kjeldsen

Subjects: “…single cell genomics…”
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Systematic evaluation of signal-to-noise ratio in variant detection from single cell genome multiple displacement amplification and exome sequencing by Anita T. Simonsen, Marcus C. Hansen, Eigil Kjeldsen, Peter L. Møller, Johnny J. Hindkjær, Peter Hokland, Anni Aggerholm

“…Abstract Background The current literature on single cell genomic analyses on the DNA level is conflicting regarding requirements for cell quality, amplification success rates, allelic dropouts and resolution, lacking a systematic comparison of multiple cell input down to the single cell. …”
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Agarose gel microcapsules enable easy-to-prepare, picolitre-scale, single-cell genomics, yielding high-coverage genome sequences by Hiroyoshi Aoki, Yuki Masahiro, Michiru Shimizu, Yuichi Hongoh, Moriya Ohkuma, Yutaka Yamagata

“…Our novel method using AGM will allow many researchers to perform single-cell genomics easily and effectively, and can accelerate genomic analysis of yet-uncultured microorganisms.…”
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Sequencing of Single Pollen Nuclei Reveals Meiotic Recombination Events at Megabase Resolution and Circumvents Segregation Distortion Caused by Postmeiotic Processes by Steven Dreissig, Jörg Fuchs, Axel Himmelbach, Martin Mascher, Martin Mascher, Andreas Houben

Subjects: “…single-cell genomics…”
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CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells by Derek H. Janssens, Dominik J. Otto, Michael P. Meers, Manu Setty, Kami Ahmad, Steven Henikoff

Subjects: “…Single-cell genomics…”
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Editorial: The Uncultured Microorganisms: Novel Technologies and Applications by Bin-Bin Xie, Meng Li, Karthik Anantharaman, Nikolai V. Ravin

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