Published 2021-06-01
“…Izabela Rumienczyk,1 Maria Kulecka,1,2 Jerzy Ostrowski,1,2 Daniel Mar,3 Karol Bomsztyk,3 Stephen W Standage,4,5 Michal Mikula1 1Maria Sklodowska-Curie National Research Institute of Oncology, Department of Genetics, Warsaw, 02-781, Poland; 2Centre for Postgraduate Medical Education, Department of Gastroenterology, Hepatology and Clinical Oncology, Warsaw, 01-813, Poland; 3UW Medicine
South Lake Union, University of Washington, Seattle, WA, 98109, USA; 4Division of Critical Care Medicine, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA; 5Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USACorrespondence: Michal MikulaMaria Sklodowska-Curie National Research Institute of Oncology, Department of Genetics, Warsaw, 02-781, PolandTel +48225462655Email michal.mikula@pib-nio.plPurpose: During sepsis, an excessive inflammatory immune reaction contributes to multi-organ dysfunction syndrome (MODS), a critical condition associated with high morbidity and mortality; however, the molecular mechanisms driving MODS remain elusive.Methods: We used RNA sequencing to characterize transcriptional changes in the early phase of sepsis, at 6, 12, 24 hour time points in lung, kidney, liver, and heart tissues, in a cecal ligation and puncture (CLP)-induced polymicrobial sepsis murine model.Results: The CLP surgery induced significant changes (adj. p-value< 0.05) in expression of hundreds of transcripts in the four organs tested, with the highest number exceeding 2,000 differentially expressed genes (DEGs) in all organs at 12 hours post-CLP. …”
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