The localization and functional analysis by Watanabe, K

“…</p> <p>In this thesis, TPC proteins have been investigated as potential NAADP receptors in the sea urchin, Strongylocentrotus purpuratus, whose genome encodes three potential TPC proteins, namely SpTPC1, SpTPC2 and SpTPC3, and in the social amoeba, Dictyostelium discoideum, which encodes only one TPC2 (DdTPC2). …”

Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes by Sadhna Phanse, Cuihong Wan, Blake Borgeson, Fan Tu, Kevin Drew, Greg Clark, Xuejian Xiong, Olga Kagan, Julian Kwan, Alexandr Bezginov, Kyle Chessman, Swati Pal, Graham Cromar, Ophelia Papoulas, Zuyao Ni, Daniel R. Boutz, Snejana Stoilova, Pierre C. Havugimana, Xinghua Guo, Ramy H. Malty, Mihail Sarov, Jack Greenblatt, Mohan Babu, W. Brent Derry, Elisabeth R. Tillier, John B. Wallingford, John Parkinson, Edward M. Marcotte, Andrew Emili

“…Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis elegans, Drosophila melanogaster, Mus musculus, Strongylocentrotus purpuratus, and Homo sapiens. The interaction network obtained from the data was validated globally in 4 distant species (Xenopus laevis, Nematostella vectensis, Dictyostelium discoideum, Saccharomyces cerevisiae) and locally by targeted affinity-purification experiments. …”
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