High risk clear cell renal cell carcinoma microenvironments contain protumour immunophenotypes lacking specific immune checkpoints by Arti M. Raghubar, Nicholas A. Matigian, Joanna Crawford, Leo Francis, Robert Ellis, Helen G. Healy, Andrew J. Kassianos, Monica S. Y. Ng, Matthew J. Roberts, Simon Wood, Andrew J. Mallett

“…Additional HG TME immunophenotype characteristics included: pro-tumour tissue-resident monocytes with consistently increased expression of HAVCR2 and LAG3 checkpoints; an exhausted CD8+ T cells sub-population with stem-like progenitor gene expression; and pro-tumour tumour-associated macrophages and monocytes within the recurrent TME with the expression of TREM2. Whilst limited by a modest sample size, this study represents the largest ST-seq dataset on human ccRCC. …”
Get full text

Microglia: A pharmacological target for the treatment of age-related cognitive decline and Alzheimer’s disease by Chloe G. McKee, Chloe G. McKee, Madison Hoffos, Madison Hoffos, Haley A. Vecchiarelli, Marie-Ève Tremblay, Marie-Ève Tremblay, Marie-Ève Tremblay, Marie-Ève Tremblay, Marie-Ève Tremblay, Marie-Ève Tremblay, Marie-Ève Tremblay

“…Preclinical and early phase clinical trials investigating the therapeutic effects of pharmacological agents acting on microglia, including reactive oxygen species, TREM2, fractalkine signaling, the complement cascade, and the NLRP3 inflammasome, are currently underway; however, important questions remain unanswered. …”
Get full text

Genome-wide association study in Alzheimer’s disease: a bibliometric and visualization analysis by Junyao Zhang, Yinuo Wang, Yingying Zhang, Junyan Yao, Junyan Yao

“…Disease and causal variants were research fronts.ConclusionThe most frequently studied AD pathogenesis and research hotspots are (1) Aβ and tau, (2) immunity and microglia, with TREM2 as a potential immunotherapy target, and (3) DNA methylation. …”
Get full text

ABCA1 haplodeficiency affects the brain transcriptome following traumatic brain injury in mice expressing human APOE isoforms by Emilie L. Castranio, Cody M. Wolfe, Kyong Nyon Nam, Florent Letronne, Nicholas F. Fitz, Iliya Lefterov, Radosveta Koldamova

“…The module that positively correlated to TBI groups was associated with immune response and featured hub genes that were microglia-specific, including Trem2, Tyrobp, Cd68 and Hexb. The modules positively correlated with APOE4 isoform and negatively to Abca1 haplodeficient mice represented “protein translation” and “oxidation-reduction process”, respectively. …”
Get full text

The miR-23-27-24 clusters drive lipid-associated macrophage proliferation in obese adipose tissue by Neil T. Sprenkle, Nathan C. Winn, Kaitlyn E. Bunn, Yang Zhao, Deborah J. Park, Brenna G. Giese, John J. Karijolich, K. Mark Ansel, C. Henrique Serezani, Alyssa H. Hasty, Heather H. Pua

“…Analysis of ATMs from these mice shows selectively reduced numbers and proliferation of a recently reported subset of lipid-associated CD9+Trem2+ ATMs (lipid-associated macrophages [LAMs]). …”
Get full text

Single-cell transcriptomic analysis reveals characteristic feature of macrophage reprogramming in liver Mallory-Denk bodies pathogenesis by Zixuan Fang, Bei Zhong, Yi Shi, Wanmei Zhou, Maoping Huang, Samuel W. French, Xiaoping Tang, Hui Liu

“…Particularly, we identified a novel Gpnmbhigh KCs subset as lipid-associated macrophage (LAM) with high expression of Trem2, CD63, and CD9. Interestingly, LAM showed a potential immunosuppressive characteristic by expressing anti-inflammatory genes IL-7R during the MDBs formation. …”
Get full text

Comprehensive analysis of shared risk genes and immunity-metabolisms between non-alcoholic fatty liver disease and atherosclerosis via bulk and single-cell transcriptome analyses by Qian Hu, Yunfang Luo, Hao He, Hua Chen, Di Liao

“…The final common risk genes (ACP5, TP53I3, RPS6KA1, TYMS, TREM2, CA12, and IFI27) were defined by intersecting the upregulated DEGs with 198 genes and validated in new datasets (GSE48452 and GSE43292). …”
Get full text

Characterization of prostate macrophage heterogeneity, foam cell markers, and CXCL17 upregulation in a mouse model of steroid hormone imbalance by Samara V. Silver, Kayah J. Tucker, Renee E. Vickman, Nadia A. Lanman, O. John Semmes, Nehemiah S. Alvarez, Petra Popovics

“…Further markers of foam cells were also identified, including Gpnmb and Trem2, and GPNMB was confirmed as a novel histological marker with immunohistochemistry. …”
Get full text

Sexual dimorphism in atherosclerotic plaques of aged Ldlr −/− mice by Virginia Smit, Jill de Mol, Mireia N. A. Bernabé Kleijn, Marie A. C. Depuydt, Menno P. J. de Winther, Ilze Bot, Johan Kuiper, Amanda C. Foks

“…In contrast, increased numbers of age-associated Gzmk+CD8+ T cells, dendritic cells, and Trem2 + macrophages were observed in male aortas. …”
Get full text

In silico-in vitro modeling to uncover cues involved in establishing microglia identity: TGF-β3 and laminin can drive microglia signature gene expression by Raissa Timmerman, Ella Alwine Zuiderwijk-Sick, Wia Baron, Jeffrey John Bajramovic, Jeffrey John Bajramovic

“…In vitro exposure to TGF-β3 increased the mRNA expression levels of the microglia signature gene TREM2. Microglia cultured on laminin-coated substrates were characterized by reduced mRNA expression levels of extracellular matrix-associated genes MMP3 and MMP7, and by increased mRNA expression levels of the microglia signature genes GPR34 and P2RY13. …”
Get full text

The correlation between triiodothyronine and the severity of liver fibrosis by Weiwei He, Caoxin Huang, Liying Wang, Weijuan Su, Shunhua Wang, Peiying Huang, Xiaofang Zhang, Yinxiang Huang, Yan Zhao, Mingzhu Lin, Xiulin Shi, Xuejun Li

“…We also found that T3 could inhibit the profibrotic TREM2+CD9+ macrophage, which had been identified an important player in the progression of liver fibrosis. …”
Get full text

Single-cell RNA-Seq reveals changes in immune landscape in post-traumatic osteoarthritis by Aimy Sebastian, Nicholas R. Hum, Jillian L. McCool, Jillian L. McCool, Stephen P. Wilson, Deepa K. Murugesh, Kelly A. Martin, Naiomy Deliz Rios-Arce, Beheshta Amiri, Blaine A. Christiansen, Gabriela G. Loots, Gabriela G. Loots

“…Further characterization of monocytes and macrophages reveled 9 major subtypes with unique transcriptomics signatures, including a tissue resident Lyve1hiFolr2hi macrophage population and Trem2hiFcrls+ recruited macrophages, both showing enrichment for phagocytic genes and growth factors such as Igf1, Pdgfa and Pdgfc. …”
Get full text

Integrative single-cell transcriptomic analyses reveal the cellular ontological and functional heterogeneities of primary and metastatic liver tumors by Menghui Gui, Shilin Huang, Shizhou Li, Yuying Chen, Furong Cheng, Yulin Liu, Ji-ao Wang, Yuting Wang, Rui Guo, Yiming Lu, Pengbo Cao, Gangqiao Zhou

“…With regard to myeloid cells, the liver-resident macrophages and inflammatory monocyte/macrophages were markedly replaced by tumor-associated macrophages (TAMs), with TREM2+ and UBE2C+ TAMs enriched in PTs, while SPP1+ and WDR45B+ TAMs in MTs. …”
Get full text

Innate Immunity Components and Cytokines in Gastric Mucosa in Children with Helicobacter pylori Infection by Jacek Michalkiewicz, Anna Helmin-Basa, Renata Grzywa, Mieczyslawa Czerwionka-Szaflarska, Anna Szaflarska-Poplawska, Grazyna Mierzwa, Andrzej Marszalek, Magdalena Bodnar, Magdalena Nowak, Katarzyna Dzierzanowska-Fangrat

“…H. pylori infection did not lead to a significant upregulation of MyD88, TLR2, TLR4, CD14, TREM1, and TREM2 mRNA expression but instead resulted in high mRNA expression of IL-6, IL-10, IFN-γ, TNF-α, and CD163. …”
Get full text

Role of TET1-mediated epigenetic modulation in Alzheimer's disease by Matthew J. Armstrong, Yulin Jin, Selina M. Vattathil, Yanting Huang, Jason P. Schroeder, David A. Bennet, Zhaohui S. Qin, Thomas S. Wingo, Peng Jin

“…RNA-Seq analysis revealed a significant increase in the expression of AD-associated genes such as Mpeg1, Ctsd, and Trem2. In conclusion, our results suggest that TET enzymes, particularly TET1, which regulate the methylome, may contribute to AD pathogenesis, as the loss of TET function increases AD-associated pathology.…”
Get full text

Integrated immunogenomic analyses of high-grade serous ovarian cancer reveal vulnerability to combination immunotherapy by Raphael Gronauer, Leonie Madersbacher, Pablo Monfort-Lanzas, Pablo Monfort-Lanzas, Gabriel Floriani, Susanne Sprung, Alain Gustave Zeimet, Christian Marth, Heidelinde Fiegl, Hubert Hackl

“…Further characterization of these samples revealed increased infiltration of immunosuppressive cells, including tumor-associated macrophages expressing TREM2, C1QA, and LILRB4, as specified by single-cell RNA sequencing data and gene expression analysis of samples from patients receiving combination therapy with PARPi and anti-PD-1. …”
Get full text

“Combo” Multi-Target Pharmacological Therapy and New Formulations to Reduce Inflammation and Improve Endogenous Remyelination in Traumatic Spinal Cord Injury by Marzia Moretti, Riccardo Caraffi, Luca Lorenzini, Ilaria Ottonelli, Michele Sannia, Giuseppe Alastra, Vito Antonio Baldassarro, Alessandro Giuliani, Jason Thomas Duskey, Maura Cescatti, Barbara Ruozi, Luigi Aloe, Maria Angela Vandelli, Luciana Giardino, Giovanni Tosi, Laura Calzà

“…A strong anti-inflammatory effect was observed in the short term with a reduction of type M1 microglia and glutamate levels, but with a subsequent increase of TREM2. In the long term, an improvement of myelination in NG2-IR, an increase in MBP content, and a reduction of the demyelination area were observed. …”
Get full text

Multiplexed Digital Spatial Protein Profiling Reveals Distinct Phenotypes of Mononuclear Phagocytes in Livers with Advanced Fibrosis by Jaejun Lee, Chang Min Kim, Jung Hoon Cha, Jin Young Park, Yun Suk Yu, Hee Jung Wang, Pil Soo Sung, Eun Sun Jung, Si Hyun Bae

“…Internal validation using mRNA expression data with the same cohort tissues demonstrated that mRNA levels for TREM2, CD9, and CD68 are significantly higher in livers with advanced fibrosis. …”
Get full text

Unveiling macrophage diversity in myocardial ischemia-reperfusion injury: identification of a distinct lipid-associated macrophage subset by Ying Jiang, Wenpeng Yu, Tie Hu, Hanzhi Peng, Fajia Hu, Yong Yuan, Xufeng Liu, Songqing Lai, Jianliang Zhou, Xiao Dong

“…Macrophage subtypes, including a novel Lipid-Associated Macrophage (LAM) subtype characterized by high expression of Spp1, Trem2, and other genes, were identified. Enrichment and Progeny pathway analyses highlighted the distinctive functional role of the SPP1+ LAM subtype, particularly in lipid metabolism and the regulation of the MAPK pathway. …”
Get full text

Induction of a distinct macrophage population and protection from lung injury and fibrosis by Notch2 blockade by Mayra Cruz Tleugabulova, Sandra P. Melo, Aaron Wong, Alexander Arlantico, Meizi Liu, Joshua D. Webster, Julia Lau, Antonie Lechner, Basak Corak, Jonathan J. Hodgins, Venkata S. Garlapati, Marco De Simone, Ben Korin, Shimrit Avraham, Jessica Lund, Surinder Jeet, Alexander Reiss, Hannah Bender, Cary D. Austin, Spyros Darmanis, Zora Modrusan, Hans Brightbill, Steffen Durinck, Michael S. Diamond, Christoph Schneider, Andrey S. Shaw, Maximilian Nitschké

“…Previously, a recruited macrophage population marked by expression of Spp1, Cd9, Gpnmb, Fabp5, and Trem2, has been described in several models of organ injury and cancer, and has been linked to fibrosis in mice and humans. …”
Get full text