Spontaneous Lymphoma in a SMP30 Knock-Out C57BL/6 Mouse by Lee Seoung-Woo, Baek Su-Min, Lee Young-Jin, Park Jin-Kyu

“…70-weeks-old female C57BL6 senescence marker protein 30 knock out mice exhibited anorexia, lethargy and enlarged abdomen because of an intraperitoneal mass. …”
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Anti-prion drugs do not improve survival in novel knock-in models of inherited prion disease by Daniel J. Walsh, Judy R. Rees, Surabhi Mehra, Matthew E. C. Bourkas, Lech Kaczmarczyk, Erica Stuart, Walker S. Jackson, Joel C. Watts, Surachai Supattapone

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40008 COMBINED CRISPR/CAS9 AND AAV FOR THE GENERATION OF CONDITIONAL ISOGENIC GENE KNOCK-INS by Prajwal Boddu, Abhishek Gupta, Todd Waldman, Manoj Pillai

“…OBJECTIVES/GOALS: Conventional approaches to creating isogenic knock-ins, to model disease-associated mutations, are limited by poor efficiency and loss of the mutant allele on extended culture. …”
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Mutation-independent gene knock-in therapy targeting 5′UTR for autosomal dominant retinitis pigmentosa by Duc Anh Hoang, Baoshan Liao, Zongli Zheng, Wenjun Xiong

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CRISPR/Cas9-mediated targeted knock-in of large constructs using nocodazole and RNase HII by Shahin Eghbalsaied, Wilfried A. Kues

“…However, the HDR-mediated targeted knock-in suffered from low efficiency. In this study, we made several large plasmids (12.1–13.4 kb) which included the CRISPR/Cas9 system along with a puromycin transgene as part of the large DNA donor (5.3–7.1 kb insertion cassettes) and used them to evaluate their targeted integration efficiency into a transgenic murine embryonic fibroblast (MEF) cell line carrying a single copy of a Venus transgene. …”
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Knee Osteoarthritis Progression Is Delayed in Silent Information Regulator 2 Ortholog 1 Knock-in Mice by Tetsuya Yamamoto, Nobuaki Miyaji, Kiminari Kataoka, Kyohei Nishida, Kanto Nagai, Noriyuki Kanzaki, Yuichi Hoshino, Ryosuke Kuroda, Takehiko Matsushita

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Recapitulating X-Linked Juvenile Retinoschisis in Mouse Model by Knock-In Patient-Specific Novel Mutation by Ding Chen, Ding Chen, Tao Xu, Tao Xu, Mengjun Tu, Mengjun Tu, Jinlin Xu, Jinlin Xu, Chenchen Zhou, Chenchen Zhou, Lulu Cheng, Lulu Cheng, Ruqing Yang, Tanchu Yang, Weiwei Zheng, Weiwei Zheng, Xiubin He, Xiubin He, Ruzhi Deng, Ruzhi Deng, Xianglian Ge, Xianglian Ge, Jin Li, Jin Li, Zongming Song, Zongming Song, Zongming Song, Junzhao Zhao, Feng Gu, Feng Gu

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Generation of epitope tag knock-in mice with CRISPR-Cas9 to study the function of endogenous proteins by Zhao Zhang

“…Here we present a protocol for generating epitope tag knock-in mice with CRISPR-Cas9-mediated gene editing. …”
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Generation, characterization, and use of EKLF(Klf1)/CRE knock-in mice for cell-restricted analyses by Li Xue, Kaustav Mukherjee, Kaustav Mukherjee, Kevin A. Kelley, Kevin A. Kelley, Kevin A. Kelley, James J. Bieker, James J. Bieker, James J. Bieker, James J. Bieker

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Knocking out alpha-synuclein in melanoma cells downregulates L1CAM and decreases motility by Nithya Gajendran, Santhanasabapathy Rajasekaran, Stephan N. Witt

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Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46 by Hann-Wei Chen, Verena Huber, Kati Szakmary-Braendle, Kerstin Seitz, Marlene Moetz, Till Ruemenapf, Christiane Riedel

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FastKnock: an efficient next-generation approach to identify all knockout strategies for strain optimization by Leila Hassani, Mohammad R. Moosavi, Payam Setoodeh, Habil Zare

“…Our implementation of the FastKnock method in Python is publicly available at https://github.com/leilahsn/FastKnock .…”
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Anti-prion drugs do not improve survival in novel knock-in models of inherited prion disease. by Daniel J Walsh, Judy R Rees, Surabhi Mehra, Matthew E C Bourkas, Lech Kaczmarczyk, Erica Stuart, Walker S Jackson, Joel C Watts, Surachai Supattapone

“…In this study, we evaluated the efficacy of various anti-prion drugs on newly-developed knock-in mouse models for FFI and fCJD. These models express bank vole prion protein (PrP) with the pathogenic D178N and E200K mutations. …”
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Enhanced Heart Failure in Redox‐Dead Cys17Ser PKARIα Knock‐In Mice by M. M. Towhidul Islam, Daniel Tarnowski, Min Zhang, Maximilian Trum, Simon Lebek, Julian Mustroph, Henriette Daniel, Johanna Moellencamp, Steffen Pabel, Samuel Sossalla, Ali El‐Armouche, Viacheslav O. Nikolaev, Ajay M. Shah, Philip Eaton, Lars S. Maier, Can Martin Sag, Stefan Wagner

“…Methods and Results We used a redox‐dead transgenic mouse model with homozygous knock‐in replacement of redox‐sensitive cysteine 17 with serine within the regulatory subunits of PKARIα (KI). …”
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Parametric Knocking Performance Investigation of Spark Ignition Natural Gas Engines and Dual Fuel Engines by La Xiang, Gerasimos Theotokatos, Haining Cui, Keda Xu, Hongkai Ben, Yu Ding

Subjects: “…knocking performance…”
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Altered Phenotypes of Breast Epithelial × Breast Cancer Hybrids after ZEB1 Knock-Out by Alexander Merckens, Mareike Sieler, Silvia Keil, Thomas Dittmar

“…Similarly, only the M13HS-8 ZEB1-knock-out tumor hybrids showed a reduced invasion capacity. …”
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Metabolic switch from fatty acid oxidation to glycolysis in knock‐in mouse model of Barth syndrome by Arpita Chowdhury, Angela Boshnakovska, Abhishek Aich, Aditi Methi, Ana Maria Vergel Leon, Ivan Silbern, Christian Lüchtenborg, Lukas Cyganek, Jan Prochazka, Radislav Sedlacek, Jiri Lindovsky, Dominic Wachs, Zuzana Nichtova, Dagmar Zudova, Gizela Koubkova, André Fischer, Henning Urlaub, Britta Brügger, Dörthe M Katschinski, Jan Dudek, Peter Rehling

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Multiple cranial organ defects after conditionally knocking out Fgf10 in the neural crest by Tathyane H.N. Teshima, Silvia V Lourenco, Abigail Saffron Tucker

“…Fgf10 is necessary for the development of a number of organs that fail to develop or are reduced in size in the null mutant. Here we have knocked out Fgf10 specifically in the neural crest driven by Wnt1cre. …”
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Neural‐Network Potential Simulation of Defect Formation Induced by Knock‐On Irradiation Damage in GaN by Chengzhen Song, Lilai Jiang, Yu‐Ning Wu, Shiyou Chen

“…Furthermore, based on the large‐scale simulations of the knock‐on process, Ga and N vacancies, as well as N interstitials are found to be the major generated defects, whereas only Ga vacancies are predicted in the previous device simulation studies that have been widely recognized. …”
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Effectiveness of conjunctival bleb scarring by knock-down of heat shock protein 47 in rat model by Wei-Wei Wang, Hai-Yan Li, Huan-Huan Yan

“…AIM: To evaluate the effectiveness of knock-down of heat shock protein 47 (HSP47) on conjunctival bleb scarring in a rat model and its possible mechanism. …”
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