Multiple <i>Clonostachys rosea</i> UDP-Glycosyltransferases Contribute to the Production of 15-Acetyl-Deoxynivalenol-3-O-Glycoside When Confronted with <i>Fusarium graminearum</i> by Kelly A. Robinson, Antony D. St-Jacques, Sam W. Shields, Amanda Sproule, Zerihun A. Demissie, David P. Overy, Michele C. Loewen

“…Using CRISPR/Cas9 technology, we knocked out several of the selected UDP-GTases in the <i>C. rosea</i> strain ACM941. …”
Get full text

Alkaline Stress Causes Changes in Polyamine Biosynthesis in <i>Thermus thermophilus</i> by Teruyuki Kobayashi, Akihiko Sakamoto, Keiko Kashiwagi, Kazuei Igarashi, Toshiyuki Moriya, Tairo Oshima, Yusuke Terui

“…The amounts of tetraamines (spermine and its homologs) markedly decreased at alkaline pH. Thus, we knocked out the <i>speE</i> gene, which is involved in the biosynthesis of tetraamines, and changes of composition of polyamines with pH changes in the mutant cells were studied. …”
Get full text

Targeting PARK7 Improves Acetaminophen-Induced Acute Liver Injury by Orchestrating Mitochondrial Quality Control and Metabolic Reprogramming by Jiao Cai, Deqin Kong, Zi Long, Jiangzheng Liu, Rui Liu, Chunxu Hai

“…PARK7 was silenced in vitro by lentiviral transfection and knocked down in vivo by AAV adeno-associated virus. …”
Get full text

The Jujube TCP Transcription Factor ZjTCP16 Regulates Plant Growth and Cell Size by Affecting the Expression of Genes Involved in Plant Morphogenesis by Qiqi Yang, Qicheng Li, Liyuan Gu, Peng Chen, Yu Zhang, Yonghua Li, Yun Chen, Xia Ye, Bin Tan, Xianbo Zheng, Jidong Li, Jiancan Feng

“…The gene functions of <i>ZjTCP16</i> were analyzed by its overexpression in <i>Arabidopsis</i> and jujube, as well as knock-down in. The overexpression of <i>ZjTCP16</i> in <i>Arabidopsis</i> and jujube resulted in dwarfism and small leaves, while the <i>zjtcp16</i> CRISPR mutants were higher than the WT. …”
Get full text

Arap1 loss causes retinal pigment epithelium phagocytic dysfunction and subsequent photoreceptor death by Andy Shao, Antonio Jacobo Lopez, JiaJia Chen, Addy Tham, Seanne Javier, Alejandra Quiroz, Sonia Frick, Edward M. Levine, K. C. Kent Lloyd, Brian C. Leonard, Christopher J. Murphy, Thomas M. Glaser, Ala Moshiri

“…In this study, we generated conditional knockout mice for Müller glia/RPE, Müller glia and RPE via targeting Rlbp1, Glast and Vmd2 promoters, respectively, to drive Cre recombinase expression to knock out Arap1. Vmd2-Cre Arap1tm1c/tm1c and Rlbp1-Cre Arap1tm1c/tm1c mice, but not Glast-Cre Arap1tm1c/tm1c mice, recapitulated the phenotype originally observed in germline Arap1−/− mice. …”
Get full text

Pharmacological and Genetic Disruption of C-Type Natriuretic Peptide (<i>nppcl</i>) Expression in Zebrafish (<i>Danio rerio</i>) Causes Stunted Growth during Development by Andrew J. Lessey, Samantha M. Mirczuk, Annisa N. Chand, Deborah M. Kurrasch, Márta Korbonits, Stijn J. M. Niessen, Craig A. McArdle, Imelda M. McGonnell, Robert C. Fowkes

“…Expression of endogenous <i>nppcl</i> was downregulated in these CNP-treated embryos, suggesting that negative feedback of the CNP system might influence growth during development. CRISPR knock-down of endogenous <i>nppcl</i> in developing zebrafish embryos also resulted in impaired growth characteristics. …”
Get full text

BDNF is altered in a brain-region specific manner and rescues deficits in Spinocerebellar Ataxia Type 1 by Juao-Guilherme Rosa, Katherine Hamel, Alyssa Soles, Carrie Sheeler, Ella Borgenheimer, Stephen Gilliat, Kaelin Sbrocco, Ferris Ghanoum, Hillary P. Handler, Colleen Forster, Orion Rainwater, Marija Cvetanovic

“…Thus, we next quantified Bdnf expression in Atxn1154Q/2Q mice, a knock-in mouse model of SCA1, during the early symptomatic disease stage in four clinically relevant brain regions: cerebellum, medulla, hippocampus and motor cortex. …”
Get full text

NADPH Oxidase 2 Mediates Myocardial Oxygen Wasting in Obesity by Anne D. Hafstad, Synne S. Hansen, Jim Lund, Celio X. C. Santos, Neoma T. Boardman, Ajay M. Shah, Ellen Aasum

“…Myocardial ROS content and mechanoenergetics were measured in the hearts from diet-induced-obese wild type (DIO_WT) and global NOK2 knock-out mice (DIO_KO) and in diet-induced obese C57BL/6J mice given normal water (DIO) or water supplemented with the NOX2-inhibitor apocynin (DIO_APO). …”
Get full text

The accumulation of modular serine protease mediated by a novel circRNA sponging miRNA increases Aedes aegypti immunity to fungus by Tengfei Lu, Yannan Ji, Mengmeng Chang, Xiaoming Zhang, Yanhong Wang, Zhen Zou

“…The miRNA-novel-53 was also knocked out using CRISPR-Cas9, which increased mosquito resistance to the fungus B. bassiana. …”
Get full text

IL-10 protects against OPC ferroptosis by regulating lipid reactive oxygen species levels post stroke by Weihua Wu, Zhaoli Luo, Danmin Shen, Ting Lan, Zhongnan Xiao, Meng Liu, Liye Hu, Tingting Sun, Yamei Wang, Jian-Nan Zhang, Chenguang Zhang, Peipei Wang, Yabin Lu, Fei Yang, Qian Li

“…In addition, in an autologous blood injection intracerebral hemorrhagic stroke (ICH) mouse model, deficiency of the endogenous Il-10, specific knocking out Il10r or Dlk1 in OPCs, or administration of ACC inhibitor was associated with increased OPC cell death, demyelination, axonal sprouting, and the cognitive deficits during the chronic phase of ICH and vice versa. …”
Get full text

mtDNA extramitochondrial replication mediates mitochondrial defect effects by Zhaoliang Shan, Shengnan Li, Yuxue Gao, Chunhua Jian, Xiuxiu Ti, Hui Zuo, Ying Wang, Guochun Zhao, Yan Wang, Qing Zhang

“…Here, we built fly mitochondrial defect models by knocking down the essential mitochondrial genes dMterf4 and dMrps23. …”
Get full text

The Constitutive Lack of α7 Nicotinic Receptor Leads to Metabolic Disorders in Mouse by Blandine Gausserès, Junjun Liu, Ewout Foppen, Cécile Tourrel-Cuzin, Ana Rodriguez Sanchez-Archidona, Etienne Delangre, Céline Cruciani-Guglielmacci, Stéphanie Pons, Uwe Maskos, Bernard Thorens, Christophe Magnan, Jamileh Movassat, Kamel Maouche

“…Methods: We used an α7 nAChR knock-out (α7^−/−) mouse model fed a standard chow diet. …”
Get full text

Efficient biosynthesis of 2-keto-D-gluconic acid by fed-batch culture of metabolically engineered Gluconobacter japonicus by Weizhu Zeng, Wen Cai, Li Liu, Guocheng Du, Jian Chen, Jingwen Zhou

“…In this study, the 2-KGA synthesis pathway was regulated to enhance production of 2-KGA and reduce accumulation of the by-products 5-keto-d-gluconic acid (5-KGA) and d-gluconic acid (D-GA) in the 2-KGA producer Gluconobacter japonicus CGMCC 1.49. Knocking out the ga5dh-1 gene from a competitive pathway and overexpressing the ga2dh-A gene from the 2-KGA synthesis pathway via homologous recombination increased the titre of 2-KGA by 63.81% in shake flasks. …”
Get full text

RGS5 Determines Neutrophil Migration in the Acute Inflammatory Phase of Bleomycin-Induced Lung Injury by Neha Sharma, Chandran Nagaraj, Bence M. Nagy, Leigh M. Marsh, Natalie Bordag, Diana Zabini, Malgorzata Wygrecka, Walter Klepetko, Elisabeth Gschwandtner, Guillem Genové, Akos Heinemann, E Kenneth Weir, Grazyna Kwapiszewska, Horst Olschewski, Andrea Olschewski

“…In line with this, RGS5 was markedly increased in murine lungs following bleomycin injury. RGS knock-out mice (RGS-/-) had preserved lung function while control mice showed significant combined ventilatory disorders three days after bleomycin application as compared to untreated control mice. …”
Get full text

Bi-allelic ATG4D variants are associated with a neurodevelopmental disorder characterized by speech and motor impairment by Marie Morimoto, Vikas Bhambhani, Nour Gazzaz, Mariska Davids, Paalini Sathiyaseelan, Ellen F. Macnamara, Jennifer Lange, Anna Lehman, Patricia M. Zerfas, Jennifer L. Murphy, Maria T. Acosta, Camille Wang, Emily Alderman, Undiagnosed Diseases Network, Sara Reichert, Audrey Thurm, David R. Adams, Wendy J. Introne, Sharon M. Gorski, Cornelius F. Boerkoel, William A. Gahl, Cynthia J. Tifft, May Christine V. Malicdan

“…Furthermore, a rescue experiment performed in an ATG4 tetra knockout cell line, in which all four ATG4 isoforms were knocked out by gene editing, showed decreased GABARAPL1 priming activity for the two ATG4D missense variants located in the cysteine protease domain required for priming, suggesting that these variants impair the function of ATG4D. …”
Get full text

Hypotrichosis with juvenile macular dystrophy: Combination of whole‐genome sequencing and genome‐wide homozygosity mapping identifies a large deletion in CDH3 initially undetected... by Amir Hossein Saeidian, Hassan Vahidnezhad, Leila Youssefian, Soheila Sotudeh, Meisam Sargazi, Sirous Zeinali, Jouni Uitto

“…While this variant was found by bioinformatics predictions to be likely pathogenic, a knock‐in mouse for this variant, made by the CRISPR/Cas, showed no disease phenotype. …”
Get full text

ZNF714 Supports Pro-Oncogenic Features in Lung Cancer Cells by Urszula Oleksiewicz, Marta Machnik, Joanna Sobocińska, Sara Molenda, Anna Olechnowicz, Anna Florczak, Mikołaj Smolibowski, Mariusz Kaczmarek

“…The transcriptomic analysis of ZNF714 knocked-down cells demonstrated deregulation of cell adhesion, migration, proliferation, apoptosis, and differentiation. …”
Get full text

Dnah9 mutant mice and organoid models recapitulate the clinical features of patients with PCD and provide an excellent platform for drug screening by Rui Zheng, Wenhao Yang, Yuting Wen, Liang Xie, Fang Shi, Danli Lu, Jiaxin Luo, Yan Li, Rui Zhang, Ting Chen, Lina Chen, Wenming Xu, Hanmin Liu

“…Bioinformatic analysis, structure simulation assay, and western blot analysis showed that the mutations affected the structure and expression of DNAH9 protein. Dnah9 knock-down (KD) mice recapitulated the patient phenotypes, including low lung function, mucin accumulation, and increased immune cell infiltration. …”
Get full text

Resistin secreted by porcine alveolar macrophages leads to endothelial cell dysfunction during Haemophilus parasuis infection by Kexin Hua, Tingting Li, Yanling He, Aohan Guan, Liying Chen, Yuan Gao, Qianshuan Xu, Haoyu Wang, Rui Luo, Ling Zhao, Hui Jin

“…By using the transwell-based cell co-culture system, we demonstrate that knocking out resistin in porcine alveolar macrophages (PAMs) dramatically attenuated endothelial monolayer damage caused by H. parasuis. …”
Get full text

Rhes depletion promotes striatal accumulation and aggregation of mutant huntingtin in a presymptomatic HD mouse model by Yongcheng Pan, Yongcheng Pan, Beisha Tang, Beisha Tang, Beisha Tang, Xiao-Jiang Li, Shihua Li, Shihua Li, Qiong Liu, Qiong Liu

“…Growing evidence suggests Rhes plays a critical role in the selective striatal degeneration in HD, but its specific function in this context remains complex and controversial.MethodsIn this study, we utilized CRISPR/Cas9 to knockdown Rhes at different disease stages through adeno-associated virus (AAV) transduction in HD knock-in (KI) mice. Immunoblotting and immunofluorescence were employed to assess the impact of Rhes depletion on mHTT levels, neuronal loss, astrogliosis and autophagy activity.ResultsRhes depletion in 22-week-old HD KI mice (representing the presymptomatic stage) led to mHTT accumulation, reduced neuronal cell staining, and increased astrogliosis. …”
Get full text