Inhibition of ferroptosis and iron accumulation alleviates pulmonary fibrosis in a bleomycin model by Zhuo Pei, Yifei Qin, Xianghui Fu, Fengfan Yang, Fei Huo, Xue Liang, Shijie Wang, Hongyong Cui, Peng Lin, Gang Zhou, Jiangna Yan, Jiao Wu, Zhi-Nan Chen, Ping Zhu

“…Finally, we specifically knocked out TFRC expression in fibroblasts in mice, and compared with those in the control mice, the symptoms of pulmonary fibrosis were reduced in the knockout mice after bleomycin induction. …”
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Retrovirus-Derived <i>RTL9</i> Plays an Important Role in Innate Antifungal Immunity in the Eutherian Brain by Fumitoshi Ishino, Johbu Itoh, Masahito Irie, Ayumi Matsuzawa, Mie Naruse, Toru Suzuki, Yuichi Hiraoka, Tomoko Kaneko-Ishino

“…In this work, we addressed the function of <i>RTL9</i> (also known as <i>SIRH10</i>). Using knock-in mice that produce RTL9-mCherry fusion protein, we examined RTL9 expression in the brain and its reaction to fungal zymosan. …”
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EBI2 Is Temporarily Upregulated in MO3.13 Oligodendrocytes during Maturation and Regulates Remyelination in the Organotypic Cerebellar Slice Model by Maria Velasco-Estevez, Nina Koch, Ilona Klejbor, Stephane Laurent, Kumlesh K. Dev, Andrzej Szutowicz, Andreas W. Sailer, Aleksandra Rutkowska

“…Organotypic cerebellar slices prepared from wild-type and cholesterol 25-hydroxylase knock-out mice were used to study remyelination following lysophosphatidylcholine (LPC)-induced demyelination. …”
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African Swine Fever Virus pE199L Induces Mitochondrial-Dependent Apoptosis by Tingting Li, Gaihong Zhao, Taoqing Zhang, Zhaoxia Zhang, Xin Chen, Jie Song, Xiao Wang, Jiangnan Li, Li Huang, Lili Wen, Changyao Li, Dongming Zhao, Xijun He, Zhigao Bu, Jun Zheng, Changjiang Weng

“…Meanwhile, cell death was markedly alleviated when the expression of pE199L was knocked down during ASFV infection. Additionally, the expression of pE199L caused a loss of mitochondrial membrane potential, release of cytochrome C, and caspase-9 and -3/7 activation, indicating that the mitochondrial apoptotic pathway was involved in pE199L-induced apoptosis. …”
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Involvement of sugar and abscisic acid in the genotype-specific response of rose to far-red light by Laurent Crespel, Camille Le Bras, Thomas Amoroso, Thomas Amoroso, Bénédicte Dubuc, Sylvie Citerne, Maria-Dolores Perez-Garcia, Soulaiman Sakr

“…Two cultivars (‘The Fairy’ – TF – and Knock Out® Radrazz – KO) with contrasting architectures were grown in a climate chamber under FR or in the absence of FR at an average photosynthetic photon flux density (400–700 nm) of 181.7 ± 12.8 μmol m−2 s−1 for 16 h. …”
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Analysis of Ana/Dfs70 Pattern in a Large Cohort of Autoimmune/Autoinflammatory Diseases Compared with First Degree Relatives and Healthy Controls Evaluated from Colombia by Consuelo Romero-Sánchez, Omar-Javier Calixto, Veronica Romero-Alvarez, Alejandra Vargas-Martin, Luis Castro, Julio Amador, Daniela Marín-Acevedo, Mónica Acevedo-Godoy, Diana Rincón-Riaño, Juan Manuel Bello-Gualtero

“…Samples with positive dense fine granular staining in the nucleoplasm of the interphase cell (AC2) fluorescence were confirmed with CytoBead/ANA and ANA/modified (Knocked out for the PSPI1 gen). Results: 530 mestizo Colombian participants were included. …”
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CD44V3, an Alternatively Spliced Form of CD44, Promotes Pancreatic Cancer Progression by Hanzhang Zhu, Weijiang Zhou, Yafeng Wan, Jun Lu, Ke Ge, Changku Jia

“…<i>CD44V3</i> and <i>U2AF1</i> were knocked down using shRNAs. The proliferation, migration, invasion, and stemness of two pancreatic cell lines, BxPC-3 and AsPC-1, were examined. …”
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TRPM7 promotes lipopolysaccharide‐induced inflammatory dysfunction in renal tubular epithelial cells by Yan Sun, Xiaobing Chen, Yongpeng Xie, Yanli Wang, Qian Zhang, Yu Lu, Xiaomin Li

“…Results TRPM7 was upregulated in LPS‐treated cells, and knocking improved cell viability, reduced LDH levels, and minimized apoptosis, inflammation, and oxidative stress. …”
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CRISPR/Cas9-Mediated Mutagenesis of <i>Antennapedia</i> in <i>Spodoptera frugiperda</i> by Congke Wang, Te Zhao, Xiaolong Liu, Tianliang Li, Leiming He, Qinqin Wang, Li Wang, Lin Zhou

“…To functionally disrupt <i>SfAntp</i>, two sgRNA sites were designed at the first exon of <i>SfAntp</i> and the gene was knocked out by CRISPR/Cas9 via microinjection. The results showed that the deletion of <i>SfAntp</i> produced a mutant phenotype of thoracic fusion, thoracic leg defect, leg-like protrusions between the head and thoracic segments and pupation deformity. …”
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LRRK2 G2019S Promotes Colon Cancer Potentially via LRRK2–GSDMD Axis-Mediated Gut Inflammation by Yuhang Wang, Joyce Z. Gao, Taylor Sakaguchi, Thorsten Maretzky, Prajwal Gurung, Nandakumar S. Narayanan, Sarah Short, Yiqin Xiong, Zizhen Kang

“…In this study, employing a colitis-associated cancer (CAC) model and LRRK2 G2019S knock-in (KI) mouse model, we demonstrate that LRRK2 G2019S promotes the pathogenesis of colon cancer, characterized by increased tumor number and size in KI mice. …”
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Melatonin enhances SIRT1 to ameliorate mitochondrial membrane damage by activating PDK1/Akt in granulosa cells of PCOS by Bo Zheng, Junan Meng, Yuan Zhu, Min Ding, Yuting Zhang, Jianjun Zhou

“…The SIRT1 was also increased with melatonin treatment, while knocking down SIRT1 mRNA inhibiting the protective effect of melatonin to activate PDK1/Akt. …”
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An FYVE-Domain-Containing Protein, PsFP1, Is Involved in Vegetative Growth, Oxidative Stress Response and Virulence of <i>Phytophthora sojae</i> by Jinhui Zhang, Xiaoran Du, Xin Zhou, Duo Jin, Jianqiang Miao, Xili Liu

“…To clarify the functions of PsFP1 and PsFP2, the CRISPR/Cas9-mediated gene replacement system was used to knock out the two genes respectively. Only heterozygous deletion mutants of <i>PsFP1</i> were recovered, and the expression level of <i>PsFP1</i> in the heterozygous knockout transformants was significantly down-regulated. …”
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Effects of TmTak1 silencing on AMP production as an Imd pathway component in Tenebrio molitor by Su Hyeon Hwang, Ho Am Jang, Maryam Ali Mohammadie Kojour, Keunho Yun, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo

“…Accordingly, AMP induction after TmTak1 knock down was mainly reported in the integument and the fat bodies.…”
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ECM1 regulates the resistance of colorectal cancer to 5-FU treatment by modulating apoptotic cell death and epithelial-mesenchymal transition induction by Sirui Long, Sirui Long, Jie Wang, Jie Wang, Fanbin Weng, Fanbin Weng, Zhigang Pei, Zhigang Pei, Shixian Zhou, Shixian Zhou, Guiyin Sun, Guiyin Sun, Debing Xiang, Debing Xiang

“…From a mechanistic perspective, knocking down and overexpressing ECM1 in HCT15/FU and HCT15 cell lines inhibited and activated PI3K/AKT/GSK3β signaling, respectively. …”
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CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of blaKPC on the IncFII conjugative plasmid by Zhiyuan Liu, Jiahao Guan, Zhaoyan Chen, Cui Tai, Zixin Deng, Yanjie Chao, Hong-Yu Ou

“…To investigate the chromosome-encoded two-component system (TCS) that regulates the carbapenem resistance of K. pneumoniae caused by blaKPC, twenty-four TCSs of a carbapenem-resistant classical K. pneumoniae ST11 clinical isolate were knocked out. The deletion mutation of the TCS regulator cpxR exhibited increased sensitivity to carbapenem, which could be restored by complementation with cpxR in trans. …”
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Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9 by Madalena C. Pinto, Margarida C. Quaresma, Iris A. L. Silva, Violeta Railean, Sofia S. Ramalho, Margarida D. Amaral

“…Our data show that SLC26A9 expression contributes to enhanced CFTR expression and function. While knocking-down SLC26A9 in human bronchial cells leads to lower wt- and F508del-CFTR expression, function, and response to CFTR correctors, the opposite occurs upon its overexpression, highlighting SLC26A9 relevance for CF. …”
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Neural control of redox response and microbiota-triggered inflammation in Drosophila gut by Naoyuki Fuse, Haruka Hashiba, Kentaro Ishibashi, Takuro Suzuki, Quang-Dat Nguyen, Kiho Fujii, Wakako Ikeda-Ohtsubo, Haruki Kitazawa, Haruki Kitazawa, Hiromu Tanimoto, Hiromu Tanimoto, Shoichiro Kurata, Shoichiro Kurata

“…In addition, we prepared samples under germ-free conditions to evaluate the role of the microbiota in gut gene expression. We knocked down the genes regulated by NP3253 neurons and examined their susceptibility to oral bacterial infections.ResultsWe found that immune-related gene expression was upregulated in NP3253 neuron-inactivated flies compared to the control. …”
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A biallelic multiple nucleotide length polymorphism explains functional causality at 5p15.33 prostate cancer risk locus by Sandor Spisak, Viktoria Tisza, Pier Vitale Nuzzo, Ji-Heui Seo, Balint Pataki, Dezso Ribli, Zsofia Sztupinszki, Connor Bell, Mersedeh Rohanizadegan, David R. Stillman, Sarah Abou Alaiwi, Alan H. Bartels, Marton Papp, Anamay Shetty, Forough Abbasi, Xianzhi Lin, Kate Lawrenson, Simon A. Gayther, Mark Pomerantz, Sylvan Baca, Norbert Solymosi, Istvan Csabai, Zoltan Szallasi, Alexander Gusev, Matthew L. Freedman

“…In LNCaP prostate cancer cells (homozygous for the 21 bp short allele), a single copy knock-in of the 47 bp long allele potently alters the chromatin state, enabling de novo functional binding of the androgen receptor (AR) associated with increased chromatin accessibility, Histone 3 lysine 27 acetylation (H3K27ac), and ~3-fold upregulation of IRX4 expression. …”
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Genome Editing Using CRISPR-Cas9 and Autoimmune Diseases: A Comprehensive Review by Min Ho Lee, Jae Il Shin, Jae Won Yang, Keum Hwa Lee, Do Hyeon Cha, Jun Beom Hong, Yeoeun Park, Eugene Choi, Kalthoum Tizaoui, Ai Koyanagi, Louis Jacob, Seoyeon Park, Ji Hong Kim, Lee Smith

“…It has been shown that CRISPR-Cas9 can be applied to knock out proprotein convertase subtilisin/kexin type 9 (PCSK9) or block PCSK9, resulting in lowering low-density lipoprotein cholesterol. …”
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Role of CRP2-MRTF interaction in functions of myofibroblasts by Ken’ichiro Hayashi, Shinri Horoiwa, Kotaro Mori, Hiroshi Miyata, Reuben Jacob Labios, Tsuyoshi Morita, Yuka Kobayashi, Chiemi Yamashiro, Fumiaki Higashijima, Takuya Yoshimoto, Kazuhiro Kimura, Yoshiaki Nakagawa

“…Here, we report that a LIM domain protein, cysteine-and glycine-rich protein 2 (CSRP2 [CRP2]) plays a vital role in the functional expression profile in myofibroblasts and cancer-associated fibroblasts (CAFs). Knock-down of CRP2 severely inhibits the expression of smooth muscle cell (SMC) genes, cell motility, and CAF-mediated collective invasion of epidermoid carcinoma. …”
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