Malonyl/Acetyltransferase (MAT) Knockout Decreases Triacylglycerol and Medium-Chain Fatty Acid Contents in Goat Mammary Epithelial Cells by Weiwei Yao, Jun Luo, Huibin Tian, Huimin Niu, Xuetong An, Xinpei Wang, Saige Zang

“…This study aimed to investigate the effects of MAT deletion on the synthesis of triacylglycerol and medium-chain fatty acids. The MAT domain was knocked out by CRISPR/Cas9 in the goat mammary epithelial cells (GMECs), and in MAT knockout GMECs, the mRNA level of FASN was decreased by approximately 91.19% and the protein level decreased by 51.83%. …”
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The contribution of preclinical magnetic resonance imaging and spectroscopy to Huntington’s disease by Jean-Baptiste Pérot, Jean-Baptiste Pérot, Emmanuel Brouillet, Julien Flament

“…Mouse models can be divided into three different constructions: transgenic mice expressing exon-1 of human huntingtin (HTT), mice with an artificial chromosome expressing full-length human HTT, and knock-in mouse models with CAG expansion inserted in the murine htt gene. …”
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The Roles of Long-Term Hyperhomocysteinemia and Micronutrient Supplementation in the AppNL–G–F Model of Alzheimer’s Disease by Hendrik Nieraad, Natasja de Bruin, Olga Arne, Martine C. J. Hofmann, Nina Pannwitz, Eduard Resch, Sonja Luckhardt, Ann-Kathrin Schneider, Sandra Trautmann, Yannick Schreiber, Robert Gurke, Robert Gurke, Michael J. Parnham, Michael J. Parnham, Uwe Till, Gerd Geisslinger, Gerd Geisslinger

“…In the present in vivo study, we attempted to further elucidate the impact of elevated homocysteine (HCys) and homocysteic acid (HCA) levels, induced by dietary B-vitamin deficiency, and micronutrient supplementation on AD-like pathology, which was simulated using the amyloid-based AppNL–G–F knock-in mouse model. For this purpose, cognitive assessment was complemented by analyses of ex vivo parameters in whole blood, serum, CSF, and brain tissues from the mice. …”
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Inhibition of FABP6 Reduces Tumor Cell Invasion and Angiogenesis through the Decrease in MMP-2 and VEGF in Human Glioblastoma Cells by Feng-Cheng Pai, Hsiang-Wei Huang, Yu-Ling Tsai, Wen-Chiuan Tsai, Yu-Chen Cheng, Hsin-Han Chang, Ying Chen

“…Finally, the bioluminescent images and immunostaining of MMP-2, cluster of differentiation 31 (CD31), and the VEGF receptor 1 (VEGFR1) revealed attenuated tumor progression in the combination of the FABP6-knocked-down and temozolomide (TMZ)-treated group in an orthotopic xenograft mouse tumor model. …”
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Integrative Multi-Omics Analysis Identifies Transmembrane p24 Trafficking Protein 1 (TMED1) as a Potential Prognostic Marker in Colorectal Cancer by Xin Guo, Wei Zhou, Jinmei Jin, Jiayi Lin, Weidong Zhang, Lijun Zhang, Xin Luan

“…Crystal violet staining indicated the cell growth was reduced after knocking down TMED1. Moreover, the flow cytometry results showed that TMED1 knockdown could increase cell apoptosis. …”
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Glycoprotein 90K Promotes E-Cadherin Degradation in a Cell Density-Dependent Manner via Dissociation of E-Cadherin–p120-Catenin Complex by So-Yeon Park, Somy Yoon, Eun Gene Sun, Rui Zhou, Jeong A. Bae, Young-Woo Seo, Jung-Il Chae, Man-Jeong Paik, Hyung-Ho Ha, Hangun Kim, Kyung Keun Kim

“…Particularly, 90K-induced E-cadherin downregulation was diminished in p120-catenin knocked-down cells. Taken together, 90K upregulation promotes the dissociation of the E-cadherin–p120-catenin complex, leading to E-cadherin proteasomal degradation, and thereby destabilizing adherens junctions in less confluent tumor cells. …”
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Reduced <i>Zeb1</i> Expression in Prostate Cancer Cells Leads to an Aggressive Partial-EMT Phenotype Associated with Altered Global Methylation Patterns by Jenna Kitz, Cory Lefebvre, Joselia Carlos, Lori E. Lowes, Alison L. Allan

“…In this study, the EMT-inducing transcription factor <i>Zeb1</i> was knocked down in mesenchymal PC-3 prostate cancer cells (Zeb1^KD) and resulting changes in cellular phenotype were assessed using protein and RNA analysis, invasion and migration assays, cell morphology assays, and DNA methylation chip analysis. …”
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Intracellular Metabolomics Identifies Efflux Transporter Inhibitors in a Routine Caco-2 Cell Permeability Assay—Biological Implications by Afia Naseem, Akos Pal, Sharon Gowan, Yasmin Asad, Adam Donovan, Csilla Temesszentandrási-Ambrus, Emese Kis, Zsuzsanna Gaborik, Gurdip Bhalay, Florence Raynaud

“…Using selective inhibitors and transporter knock-out (KO) cells and a targeted Liquid Chromatography tandem Mass Spectrometry (LC-MS) method, we identified 11 metabolites increased in cells with depleted P-glycoprotein (Pgp) activity. …”
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New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease by Mariia A. Misiurina, Angelina V. Chirinskaite, Aleksandra S. Fotina, Andrey A. Zelinsky, Julia V. Sopova, Elena I. Leonova

“…High specificity allows us to type single nucleotide substitutions and small deletions/insertions (1–2 nucleotides) and look for target mutations when knocking out.…”
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Single-Cell Transcriptomic Profiling of the Mouse Testicular Germ Cells Reveals Important Role of Phosphorylated GRTH/DDX25 in Round Spermatid Differentiation and Acrosome Biogenes... by Raghuveer Kavarthapu, Rajakumar Anbazhagan, Soumitra Pal, Maria L. Dufau

“…In germ cells of male mice, there are two species of GRTH, a 56 kDa non-phosphorylated form and 61 kDa phosphorylated form (pGRTH). GRTH Knock-In (KI) mice with R242H mutation abolished pGRTH and its absence leads to infertility. …”
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Transcriptional landscape of Kaposi sarcoma tumors identifies unique immunologic signatures and key determinants of angiogenesis by Ramya Ramaswami, Takanobu Tagawa, Guruswamy Mahesh, Anna Serquina, Vishal Koparde, Kathryn Lurain, Sarah Dremel, Xiaofan Li, Ameera Mungale, Alex Beran, Zoe Weaver Ohler, Laura Bassel, Andrew Warner, Ralph Mangusan, Anaida Widell, Irene Ekwede, Laurie T. Krug, Thomas S. Uldrick, Robert Yarchoan, Joseph M. Ziegelbauer

“…In these models, KSHV infection of LECs led to increased tubule formation that was impaired upon knock-down of STC1 or FLT4. Conclusions This study of transcriptional profiling of KS tissue provides novel insights into the characteristics and pathogenesis of this unique virus-driven neoplasm.…”
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Slc26a1 is not essential for spermatogenesis and male fertility in mice by Zhixiang Meng, Yu Qiao, Jiajia Xue, Tiantian Wu, Wenxin Gao, Xiaoyan Huang, Jinxing Lv, Mingxi Liu, Cong Shen

“…This effect was not found in mouse GC-1 and GC-2 germ cell lines with the Slc26a1 gene transiently knocked down. This result may indicate that a gene compensation phenomenon was present in the testes of Slc26a1-KO mice.…”
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The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against <i>Fusarium pseudograminearum</i> in Wheat by Feng Xiong, Xiuliang Zhu, Changsha Luo, Zhixiang Liu, Zengyan Zhang

“…Functional analysis indicated that knock down of <i>TaAACT1</i> impaired resistance against <i>F. pseudograminearum</i> and reduced the expression of downstream defense genes in wheat. …”
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A novel loss-of-function mutation in NRAP is associated with left ventricular non-compaction cardiomyopathy by Zhongman Zhang, Kangkang Xu, Lianfu Ji, Han Zhang, Jie Yin, Ming Zhou, Chunli Wang, Shiwei Yang

“…In the zebrafish model, the heart development was affected while knocking out the NRAP gene, which showed pericardial edema. …”
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Disease-associated oligodendrocyte signatures are spatiotemporally dysregulated in spinocerebellar ataxia type 3 by Kristen H. Schuster, Danielle M. DiFranco, Alexandra F. Putka, Alexandra F. Putka, Juan P. Mato, Juan P. Mato, Sabrina I. Jarrah, Nicholas R. Stec, Vikram O. Sundararajan, Hayley S. McLoughlin

“…Translating these findings to SCA3 mouse models of disease, we confirmed endogenous expression of mutant Atxn3 leads to regional transcriptional dysregulation of oligodendrocyte maturation markers in Knock-In models of SCA3. We then investigated the spatiotemporal progression of mature oligodendrocyte transcriptional dysregulation in an overexpression SCA3 mouse model and how it relates to the onset of motor impairment. …”
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Effects of Paraquat, Dextran Sulfate Sodium, and Irradiation on Behavioral and Cognitive Performance and the Gut Microbiome in A53T and A53T-L444P Mice by Ariel Chaklai, Abigail O’Neil, Shrey Goel, Nick Margolies, Destine Krenik, Ruby Perez, Kat Kessler, Elizabeth Staltontall, Hong Ki (Eric) Yoon, Montzerrat Pantoja, Keaton Stagaman, Kristin Kasschau, Vivek Unni, Robert Duvoisin, Thomas Sharpton, Jacob Raber

“…Heterozygous mice carrying one allele with the L444P mutation knocked-into the mouse gene show increased aSyn levels and are more sensitive to motor deficits following exposure to the neurotoxin (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) MPTP than wild-type mice. …”
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SMAD2 Inactivation Inhibits CLDN6 Methylation to Suppress Migration and Invasion of Breast Cancer Cells by Yan Lu, Liping Wang, Hairi Li, Yanru Li, Yang Ruan, Dongjing Lin, Minlan Yang, Xiangshu Jin, Yantong Guo, Xiaoli Zhang, Chengshi Quan

“…Upregulation of CLDN6 inhibited epithelial to mesenchymal transition (EMT) and reduced the migration and invasion ability of both MCF-7 and SKBR-3 cells. Furthermore, knocked down of CLDN6 abolished SB431542 effects on suppression of EMT associated gene expression and inhibition of migration and invasion. …”
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Savory Signaling: T1R Umami Receptor Modulates Endoplasmic Reticulum Calcium Store Content and Release Dynamics in Airway Epithelial Cells by Derek B. McMahon, Jennifer F. Jolivert, Li Eon Kuek, Nithin D. Adappa, James N. Palmer, Robert J. Lee

“…We found that both T1R1 and T1R3 detect amino acids and signal via cAMP, increasing the responsiveness of the cells to Ca²⁺ signaling stimuli. Either knocking down T1R1/3 or treating wild-type cells with MEM amino acids caused a reduction in ER Ca²⁺ content through a non-cAMP signaled pathway. …”
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Oleic acid stimulation of amino acid uptake in primary human trophoblast cells is mediated by phosphatidic acid and mTOR signaling by Elena Silva, Véronique Ferchaud‐Roucher, Anita Kramer, Lana Madi, Priyadarshini Pantham, Stephanie Chassen, Thomas Jansson, Theresa L. Powell

“…We found that inhibition of mTOR complex 1 or 2, using small interfering RNA to knock down raptor or rictor, prevented OA‐stimulated System A amino acid transport indicating the interaction of OA with mTOR. …”
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Minimal expression of dysferlin prevents development of dysferlinopathy in dysferlin exon 40a knockout mice by Joe Yasa, Claudia E. Reed, Adam M. Bournazos, Frances J. Evesson, Ignatius Pang, Mark E. Graham, Jesse R. Wark, Brunda Nijagal, Kim H. Kwan, Thomas Kwiatkowski, Rachel Jung, Noah Weisleder, Sandra T. Cooper, Frances A. Lemckert

“…Herein we use CRISPR/Cas9 gene editing to knock out murine Dysf exon 40a, to specifically assess its role in membrane repair and development of dysferlinopathy. …”
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