Upregulation of EphA4 deteriorate brain damage by shifting microglia M1-polarization via NF-κB signaling after focal cerebral ischemia in rats by Hui-Xing Wei, Yun-Ni Guan, Ping-Ping Chen, Zhao-Zeng Rao, Jin-Shan Yang

“…We manipulated EphA4/ephrin signaling with either EphA4 overexpression lentiviral vectors or the short hairpin RNA (shRNA) to upregulate or knock down neuronal EphA4 expression. NF-κB inhibitor pyrrolidine dithiocarbamate ammonium salt (PDTC) was applied to block NF-κB pathway. …”
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The Natural Chemotherapeutic Capsaicin Activates AMPK through LKB1 Kinase and TRPV1 Receptors in Prostate Cancer Cells by Belén G. Sánchez, Alicia Bort, José M. Mora-Rodríguez, Inés Díaz-Laviada

“…Capsaicin-induced LKB1 phosphorylation was dependent on the transient receptor potential cation channel subfamily V member 1 (TRPV1), since TRPV1 knocked down by shRNA abolished LKB1 and AMPK phosphorylation in LKB1-expressing cells. …”
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IL-37 Targets TSLP-Primed Basophils to Alleviate Atopic Dermatitis by Tianheng Hou, Miranda Sin-Man Tsang, Lea Ling-Yu Kan, Peiting Li, Ida Miu-Ting Chu, Christopher Wai-Kei Lam, Chun-Kwok Wong

“…In CRISPR/Cas9 human IL-37b knock-in mice or mice with direct treatment by human IL-37b antibody, AD symptoms including ear swelling and itching were significantly alleviated upon MC903 challenge. …”
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Markerless gene deletion in Ralstonia solanacearum based on its natural transformation competence by Jinli Yan, Jinli Yan, Nuoqiao Lin, Nuoqiao Lin, Xiaoqing Wang, Xiaoqing Wang, Xuemei Chen, Xuemei Chen, Huishan Wang, Huishan Wang, Qiqi Lin, Qiqi Lin, Xiaofan Zhou, Lianhui Zhang, Lianhui Zhang, Lisheng Liao

“…Using this method, we knocked out the epsB and phcA genes, which are associated with exopolysaccharide (EPS) biosynthesis and regulation, respectively, in several R. solanacearum strains isolated from different host plants at a frequency from 5 (1E-08) to 45 (1E-08). …”
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MLKL and CaMKII Are Involved in RIPK3-Mediated Smooth Muscle Cell Necroptosis by Ting Zhou, Elise DeRoo, Huan Yang, Amelia Stranz, Qiwei Wang, Roman Ginnan, Harold A. Singer, Bo Liu

“…SiRNA silencing of <i>Mlkl</i>-diminished cell death and administration of the CaMKII inhibitor myristoylated autocamtide-2-related inhibitory peptide (Myr-AIP) or siRNAs against <i>Camk2d</i> partially inhibited necroptosis. Moreover, knocking down <i>Mlkl</i> decreased CaMKII phosphorylation, but silencing <i>Camk2d</i> did not affect phosphorylation, oligomerization, or trafficking of MLKL. …”
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Nickel and cobalt resistance properties of Sinorhizobium meliloti isolated from Medicago lupulina growing in gold mine tailing by Zhefei Li, Xiuyong Song, Juanjuan Wang, Xiaoli Bai, Engting Gao, Gehong Wei

“…Pot experiments were carried out to determine whether the growth of M. lupulina was affected when the dmeF gene was knocked out in the presence of nickel or cobalt. Results indicated that the nodule number of the host plant inoculated with the dmeF deletion mutant was significantly less than the S. meliloti CCNWSX0020 wild-type in the presence of Co2 + or Ni2 +. …”
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Integrin α3 negative podocytes: A gene expression study by L.H. Frommherz, S.B. Sayar, Y. Wang, L.K. Trefzer, Y. He, J. Leppert, P. Eßer, C. Has

“…Renal involvement mainly consists of glomerular disease caused by loss of adhesion between podocytes and the glomerular basement membrane.The aim of this study was to characterize the impact of loss of integrin α3 on human podocytes.ITGA3 was stably knocked-out in the human podocyte cell line AB8/13, designated as PodoA3−, and in human proximal tubule epithelial cell line HK2 using the targeted genome editing technique CRISPR/Cas9. …”
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PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1 Proteostasis by Jason A. Kirkbride, Garbo Young Nilsson, Jee In Kim, Kosuke Takeya, Yoshinori Tanaka, Hiroshi Tokumitsu, Futoshi Suizu, Masumi Eto

“…In this study, we demonstrate that PHI-1, an endogenous inhibitor of protein phosphatase-1 (PP1), plays a pivotal role in modulating Raf-1 proteostasis within cells. Knocking down endogenous PHI-1 in HEK293 cells using siRNA resulted in increased cell proliferation and reduced apoptosis. …”
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Ventral hippocampal diacylglycerol lipase-alpha deletion decreases avoidance behaviors and alters excitation-inhibition balance by Veronika Kondev, Rebecca Bluett, Mustafa Najeed, Luis E. Rosas-Vidal, Brad A. Grueter, Sachin Patel

“…Here, we use a conditional knock-out of the 2-AG synthesis enzyme, diacylglycerol lipase α (DAGLα), to study the role of vHPC 2-AG signaling in the regulation of affective behavior. …”
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Melatonin Reduces NLRP3 Inflammasome Activation by Increasing α7 nAChR-Mediated Autophagic Flux by Víctor Farré-Alins, Paloma Narros-Fernández, Alejandra Palomino-Antolín, Céline Decouty-Pérez, Ana Belen Lopez-Rodriguez, Esther Parada, Alicia Muñoz-Montero, Vanessa Gómez-Rangel, Francisco López-Muñoz, Eva Ramos, Águeda González-Rodríguez, Luis Gandía, Alejandro Romero, Javier Egea

“…In cultures from α7 nAChR knock-out (KO) mice, melatonin did not reduce IL-1β release. …”
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Absence of Heme Oxygenase-1 Affects Trophoblastic Spheroid Implantation and Provokes Dysregulation of Stress and Angiogenesis Gene Expression in the Uterus by Maria Laura Zenclussen, Sina Ulrich, Mario Bauer, Beate Fink, Ana Claudia Zenclussen, Anne Schumacher, Nicole Meyer

“…Using siRNA, HO-1 was knocked down in JEG-3 and BeWo cells and trophoblastic spheroids were generated with or without CO treatment. …”
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Apolipoprotein A5 ameliorates MCT induced pulmonary hypertension by inhibiting ER stress in a GRP78 dependent mechanism by Jingyuan Chen, Jun Luo, Haihua Qiu, Yi Tang, Xiaojie Yang, Yusi Chen, Zilu Li, Jiang Li

“…ApoA5 could inhibit PDGF-BB-induced PASMC proliferation and endoplasmic reticulum stress by increasing the expression of glucose-regulated protein 78 (GRP78). After knocking down GRP78, the protecting effects of ApoA5 have been blocked. …”
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MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells by Xiao Hong Yang, Kun Yang, Yu Lin An, Li Bo Wang, Guo Luo, Xiao Hua Hu

“…Utilizing an osteoporosis mouse model, we demonstrated that MSCs from ovariectomized (OVX) mouse mandibular bone marrow exhibited impaired osteogenic and excessive adipogenic differentiation. miR-705 was found overexpressed in OVX mandibular MSCs. The knock down of miR-705 in vitro partially attenuated the differentiation disorder of the OVX mandibular MSCs by upregulating the expression of osteogenic marker genes but suppressing adipogenic genes. …”
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Aldehyde Dehydrogenase 2 Ameliorates Chronic Alcohol Consumption-Induced Atrial Fibrillation through Detoxification of 4-HNE by Lung-An Hsu, Feng-Chun Tsai, Yung-Hsin Yeh, Chi-Jen Chang, Chi-Tai Kuo, Wei-Jan Chen, Hsin-Yi Tsai, Gwo-Jyh Chang

“…We tested the effect of ALDH2 deficiency on alcohol-induced AF in a murine model of chronic-binge ethanol feeding, with <i>ALDH2*2</i> knock-in (KI) mice generated by a CRISPR/CAS9 system. …”
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Treatment with the dual-incretin agonist DA-CH5 demonstrates potent therapeutic effect in a rat model of Wolfram Syndrome by Toomas Jagomäe, Nayana Gaur, Kadri Seppa, Riin Reimets, Marko Pastak, Mihkel Plaas, Allen Kaasik, Eero Vasar, Mario Plaas

“…Dual agonists that target GLP-1 and glucose-dependent insulinotropic polypeptide (GIP-1) are reportedly more efficacious; hence, we evaluated the therapeutic potential of dual incretin agonism in a loss-of-function rat model of WS.MethodsEight-month-old Wfs1 knock-out (KO) and wild-type control rats were continuously treated with either the dual agonist DA-CH5 or saline for four months. …”
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GIMAP7 inhibits epithelial‐mesenchymal transition and glycolysis in lung adenocarcinoma cells via regulating the Smo/AMPK signaling pathway by Liyuan Cui, Yumei Shen, Shanzhou Duan, Qifeng Ding, Yifei Wang, Wentao Yang, Yongbing Chen

“…Methods GIMAP7 was overexpressed by transfection with GIMAP7 plasmid, and knocked down using siRNAs. The biological functions of GIMAP7 were examined by employing CCK‐8, EdU, colony formation, flow cytometry, wound healing, and transwell assays. …”
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Dysregulated Brain Protein Phosphorylation Linked to Increased Human Tau Expression in the hTau Transgenic Mouse Model by Isidro Ferrer, Pol Andrés-Benito, Karina Ausín, Paz Cartas-Cejudo, Mercedes Lachén-Montes, José Antonio del Rio, Joaquín Fernández-Irigoyen, Enrique Santamaría

“…Altered protein phosphorylation is a major pathologic modification in tauopathies and Alzheimer’s disease (AD) linked to abnormal tau fibrillar deposits in neurofibrillary tangles (NFTs) and pre-tangles and β-amyloid deposits in AD. hTau transgenic mice, which express 3R and less 4R human tau with no mutations in a murine knock-out background, show increased tau deposition in neurons but not NFTs and pre-tangles at the age of nine months. …”
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Regulation of the Expression, Oligomerisation and Signaling of the Inhibitory Receptor CLEC12A by Cysteine Residues in the Stalk Region by Julien Vitry, Guillaume Paré, Andréa Murru, Xavier Charest-Morin, Halim Maaroufi, Kenneth R. McLeish, Paul H. Naccache, Maria J. Fernandes

“…Reduced CLEC12A expression enhances myeloid cell activation and inflammation in CLEC12A knock-out mice with collagen antibody-induced or gout-like arthritis. …”
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TrkB-dependent regulation of molecular signaling across septal cell types by Lionel A. Rodriguez, Matthew Nguyen Tran, Renee Garcia-Flores, Seyun Oh, Robert A. Phillips, Elizabeth A. Pattie, Heena R. Divecha, Sun Hong Kim, Joo Heon Shin, Yong Kyu Lee, Carly Montoya, Andrew E. Jaffe, Leonardo Collado-Torres, Stephanie C. Page, Keri Martinowich

“…To better understand molecular mechanisms by which TrkB signaling controls behavior, we locally knocked down TrkB in LS and used bulk RNA-sequencing to identify changes in gene expression downstream of TrkB. …”
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The Influence of <i>Trctf1</i> Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by <i>Trichoderma reesei</i> with Various Soluble Inducers by Yudian Chen, Yushan Gao, Zancheng Wang, Nian Peng, Xiaoqin Ran, Tingting Chen, Lulu Liu, Yonghao Li

“…This study demonstrated that <i>T. reesei</i> ΔTrctf1 with the <i>Trctf1</i> gene knocked out showed no statistically significant differences in cellulase, cellobiohydrolase, endoglucanase, and β−glucosidase production when induced with MGD (the mixture of glucose and sophorose). …”
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