A zebrafish model of Ifih1-driven Aicardi–Goutières syndrome reproduces the interferon signature and the exacerbated inflammation of patients by Beatriz Bernal-Bermúdez, Beatriz Bernal-Bermúdez, Beatriz Bernal-Bermúdez, Alicia Martínez-López, Alicia Martínez-López, Francisco J. Martínez-Morcillo, Francisco J. Martínez-Morcillo, Francisco J. Martínez-Morcillo, Sylwia D. Tyrkalska, Sylwia D. Tyrkalska, Sylwia D. Tyrkalska, Teresa Martínez-Menchón, Teresa Martínez-Menchón, Teresa Martínez-Menchón, Pablo Mesa-del-Castillo, Pablo Mesa-del-Castillo, Pablo Mesa-del-Castillo, María L. Cayuela, María L. Cayuela, María L. Cayuela, Victoriano Mulero, Victoriano Mulero, Victoriano Mulero, Diana García-Moreno, Diana García-Moreno

“…Using the unique advantages of the zebrafish model for gene editing, we have generated Tg(ifih1_mut) knocked down for mavs and ikbke, which completely abrogated the Poly I:C induction and activation of the GFP of the reporters. …”
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HT-B and S-RNase CRISPR-Cas9 double knockouts show enhanced self-fertility in diploid Solanum tuberosum by Sarah Lee, Felix E. Enciso-Rodriguez, William Behling, Thilani Jayakody, Kaela Panicucci, Daniel Zarka, Satya Swathi Nadakuduti, C. Robin Buell, Norma C. Manrique-Carpintero, David S. Douches

“…The S-RNase and HT genes have been shown previously to contribute to GSI in the Solanaceae family and self-compatible S. tuberosum lines have been generated by knocking out S-RNase gene with CRISPR-Cas9 gene editing. …”
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Interdependent Impact of Lipoprotein Receptors and Lipid-Lowering Drugs on HCV Infectivity by Francisco J. Zapatero-Belinchón, Rina Ötjengerdes, Julie Sheldon, Benjamin Schulte, Belén Carriquí-Madroñal, Graham Brogden, Laura M. Arroyo-Fernández, Florian W. R. Vondran, Benjamin Maasoumy, Thomas von Hahn, Gisa Gerold

“…We found that individual ablation of the lipoprotein receptors SR-B1 and LDLr did not drastically affect HCV entry, replication, or infection, but double lipoprotein receptor knock-outs significantly reduced HCV infection. Furthermore, we could show that this effect was neither due to altered expression of additional HCV entry factors nor caused by changes in cellular cholesterol content. …”
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Loss of function of CMPK2 causes mitochondria deficiency and brain calcification by Miao Zhao, Hui-Zhen Su, Yi-Heng Zeng, Yuan Sun, Xin-Xin Guo, Yun-Lu Li, Chong Wang, Zhi-Yuan Zhao, Xue-Jing Huang, Kai-Jun Lin, Zi-Ling Ye, Bi-Wei Lin, Shunyan Hong, Jitan Zheng, Yao-Bin Liu, Xiang-Ping Yao, Dehao Yang, Ying-Qian Lu, Hai-Zhu Chen, Erwei Zuo, Guang Yang, Hong-Tao Wang, Chen-Wei Huang, Xiao-Hong Lin, Zhidong Cen, Lu-Lu Lai, Yan-Ke Zhang, Xi Li, Tianmin Lai, Jingjing Lin, Dan-Dan Zuo, Min-Ting Lin, Chia-Wei Liou, Qing-Xia Kong, Chuan-Zhu Yan, Zhi-Qi Xiong, Ning Wang, Wei Luo, Cui-Ping Zhao, Xuewen Cheng, Wan-Jin Chen

“…Notably, calcification developed in a progressive manner in the homozygous Cmpk2-KO mice thalamus region as well as in the Cmpk2-knock-in mice bearing the patient mutation, thus phenocopying the calcification pathology observed in the patients. …”
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Evidence for Electron Transfer from the Bidirectional Hydrogenase to the Photosynthetic Complex I (NDH-1) in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803 by Jens Appel, Sean Craig, Marius Theune, Vanessa Hüren, Sven Künzel, Björn Forberich, Samantha Bryan, Kirstin Gutekunst

“…We analyzed hydrogenase activity, photohydrogen evolution and hydrogen uptake, the respiration and photosynthetic electron transport of Δ<i>hoxEFUYH</i>, and a knock-out strain with dysfunctional NDH-1 (Δ<i>ndhD1</i>/Δ<i>ndhD2</i>) of the cyanobacterium <i>Synechocystis</i> sp. …”
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High-precision mapping reveals rare N 6 -deoxyadenosine methylation in the mammalian genome by Li-Qian Chen, Zhang Zhang, Hong-Xuan Chen, Jian-Fei Xi, Xue-Hong Liu, Dong-Zhao Ma, Yu-Hao Zhong, Wen Hui Ng, Tao Chen, Daniel W. Mak, Qi Chen, Yao-Qing Chen, Guan-Zheng Luo

“…In contrast to bacteria and green algae, human cells possess a very limited number of 6mA sites which are sporadically distributed across the genome of different cell types. After knocking out the RNA m6A methyltransferase METTL3 in mouse ES cells, 6mA becomes mostly diminished. …”
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Maintenance of mouse trophoblast stem cells in KSR-based medium allows conventional 3D culture by Shuai SUN, Shota YANO, Momo O NAKANISHI, Michiko HIROSE, Kazuhiko NAKABAYASHI, Kenichiro HATA, Atsuo OGURA, Satoshi TANAKA

“…MEFs can be replaced by a 70% MEF-conditioned medium (MEF-CM) or by TGF-ß/activin A. To find out if KnockOutTM Serum Replacement (KSR) can replace FBS for TSC maintenance, we cultured mouse TSCs in KSR-based, FBS-free medium and investigated their proliferation capacity, stemness, and differentiation potential. …”
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Modified DNA vaccine confers improved humoral immune response and effective virus protection against SARS-CoV-2 delta variant by Hiroki Hayashi, Jiao Sun, Yuka Yanagida, Takako Otera, Miwa Sasai, Chin Yang Chang, Jiayu A. Tai, Tomoyuki Nishikawa, Kunihiko Yamashita, Naoki Sakaguchi, Shota Yoshida, Satoshi Baba, Munehisa Shimamura, Sachiko Okamoto, Yasunori Amaishi, Hideto Chono, Junichi Mineno, Hiromi Rakugi, Ryuichi Morishita, Masahiro Yamamoto, Hironori Nakagami

“…Furthermore, the PJI-injected GP∆-delta DNA vaccine effectively protected human angiotensin-converting enzyme 2 (hACE2) knock-in mice from delta-variant infection. These results indicate that the improved DNA vaccine was effective against emerging VOCs and was a potential DNA vaccine platform for future VOCs or global pandemics.…”
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An efficient virus‐induced gene silencing (VIGS) system for gene functional studies in Miscanthus by Guo He, Xuhong Zhao, Yan Xu, Yu Wang, Zhihai Zhang, Liang Xiao, Matthew Hudson, Ruibo Hu, Shengjun Li

“…Expression of MsMYB112 was successfully knocked down using the VIGS system, and this led to compromised salt tolerance in the silenced Miscanthus plants. …”
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The Wall-Associated Receptor-Like Kinase TaWAK7D Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in Wheat by Haijun Qi, Xiuliang Zhu, Feilong Guo, Liangjie Lv, Zengyan Zhang

“…Additionally, <i>TaWAK7D</i> transcript levels were significantly elevated by pectin and chitin treatments. The knock-down of <i>TaWAK7D</i> transcript impaired resistance to <i>R. cerealis</i> and repressed the expression of five pathogenesis-related genes in wheat. …”
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Alternative Splicing of lncRNAs From SNHG Family Alters snoRNA Expression and Induces Chemoresistance in HepatoblastomaSummary by Ni Zhen, Jiabei Zhu, Siwei Mao, Qi Zhang, Song Gu, Ji Ma, Yue Zhang, Minzhi Yin, Haojie Li, Nan Huang, Han Wu, Fenyong Sun, Binwu Ying, Lin Zhou, Qiuhui Pan

“…Functionally, PABPN1 acts as an oncofetal splicing regulator in HB by promoting cell proliferation and DNA damage repair via inducing the intron retention of SNHG19. Knock-down of PABPN1 increases the cisplatin sensitivity of HB PDOs. …”
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Phosphorylation bar-coding of free fatty acid receptor 2 is generated in a tissue-specific manner by Natasja Barki, Laura Jenkins, Sara Marsango, Domonkos Dedeo, Daniele Bolognini, Louis Dwomoh, Aisha M Abdelmalik, Margaret Nilsen, Manon Stoffels, Falko Nagel, Stefan Schulz, Andrew B Tobin, Graeme Milligan

“…Using both wild-type human FFAR2 and a designer receptor exclusively activated by designer drug (DREADD) variant we explored the activation and phosphorylation profile of the receptor, both in heterologous cell lines and in tissues from transgenic knock-in mouse lines expressing either human FFAR2 or the FFAR2-DREADD. …”
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Conditional Up-Regulation of SERCA2a Exacerbates RyR2-Dependent Ventricular and Atrial Arrhythmias by Bin Liu, Qing Lou, Heather Smith, Florencia Velez-Cortes, Wolfgang H. Dillmann, Björn C. Knollmann, Antonis A. Armoundas, Sándor Györke

“…In this study, we examined the effect of acute upregulation of SERCA2a on arrhythmogenesis by conditionally overexpressing SERCA2a in a mouse model featuring hyperactive RyR2s due to ablation of calsequestrin 2 (CASQ2). CASQ2 knock-out (KO) mice were crossbred with doxycycline (DOX)-inducible SERCA2a transgenic mice to generate KO-TG mice. …”
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Dimethyl fumarate alleviates allergic asthma by strengthening the Nrf2 signaling pathway in regulatory T cells by Yanhong Cen, Yanhong Cen, Yanhong Cen, Yanhong Cen, Yanhong Cen, Yanhong Cen, Fangfang Li, Yikui Li, Kaimin Zhang, Farooq Riaz, Kuaile Zhao, Kuaile Zhao, Kuaile Zhao, Kuaile Zhao, Ping Wei, Ping Wei, Fan Pan

“…Meanwhile, DMF failed to exert protective effects on OVA-induced allergic asthma in CD4-specific Nrf2 knock-out mice. Overall, our study illustrates that DMF enhances Nrf2 signaling in T cells to assist the differentiation of Tregs, which could improve the anti-inflammatory immune response in allergic asthma.…”
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Mutations in the Vicinity of the IRAK3 Guanylate Cyclase Center Impact Its Subcellular Localization and Ability to Modulate Inflammatory Signaling in Immortalized Cell Lines by Ilona Turek, Trang H. Nguyen, Charles Galea, Isaiah Abad, Lubna Freihat, David T. Manallack, Tony Velkov, Helen Irving

“…Mutant IRAK3 variants with reduced cGMP generating capacity and differential regulation of NFκB activity influence subcellular localization of IRAK3 in HEK293T cells and fail to rescue IRAK3 function in <i>IRAK3</i> knock-out THP-1 monocytes stimulated with LPS unless the cGMP analog is present. …”
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Impact of the <i>Monocarboxylate Transporter-1</i> (MCT1)-Mediated Cellular Import of Lactate on Stemness Properties of Human Pancreatic Adenocarcinoma Cells by Leontine Sandforth, Nourhane Ammar, Lisa Antonia Dinges, Christoph Röcken, Alexander Arlt, Susanne Sebens, Heiner Schäfer

“…These lactate dependent effects on stemness properties are abrogated by the MCT1/lactate-uptake inhibitor 7ACC2 or MCT1 knock-down. Furthermore, the clinical relevance of these observations was supported by detecting co-expression of MCT1 and reprogramming factors in human PDAC tissues. …”
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Arabidopsis thaliana Early Foliar Proteome Response to Root Exposure to the Rhizobacterium Pseudomonas simiae WCS417 by Francesca Marzorati, Rossana Rossi, Letizia Bernardo, Pierluigi Mauri, Dario Di Silvestre, Emmanuelle Lauber, Laurent D. Noël, Irene Murgia, Piero Morandini

“…To elucidate early foliar responses to WCS417 as well as FDH involvement, the roots of A. thaliana wild-type Col and atfdh1-5 knock-out mutant plants grown in soil were exposed to WCS417, and proteins from rosette leaves were subjected to proteomic analysis. …”
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The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii by Tingting Guo, Tingting Guo, Tingting Guo, Xiaoli Sun, Mengying Li, Mengying Li, Yuhang Wang, Hongmei Jiao, Guocai Li, Guocai Li, Guocai Li

“…Thus, the RecAb homologous recombination system was utilized to knock out the csy1 gene from A. baumannii AB43, which carries the Type I-Fb CRISPR-Cas system, and to observe the drug resistance changes in wild-type and csy1-deleted strains. …”
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LncRNA UCA1 promotes keratinocyte-driven inflammation via suppressing METTL14 and activating the HIF-1α/NF-κB axis in psoriasis by Yibo Hu, Li Lei, Ling Jiang, Hongliang Zeng, Yushan Zhang, Chuhan Fu, Haoran Guo, Yumeng Dong, Yujie Ouyang, Xiaolin Zhang, Jinhua Huang, Qinghai Zeng, Jing Chen

“…We also observed a direct interaction between UCA1 and N6-methyladenosine (m6A) methyltransferase METTL14. Knocking down METTL14 counteracted the effects of UCA1 silencing, indicating that it can suppress inflammation. …”
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Engineered extracellular vesicles for concurrent Anti-PDL1 immunotherapy and chemotherapy by Yundi Chen, Lixue Wang, Mingfeng Zheng, Chuandong Zhu, Guosheng Wang, Yiqiu Xia, Ethan J. Blumenthal, Wenjun Mao, Yuan Wan

“…This was accomplished by overexpressing a high-affinity variant human PD-1 protein (havPD-1), while simultaneously knocking out intrinsic PD-L1 and beta-2 microglobulin. …”
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