The p23 of Citrus Tristeza Virus Interacts with Host FKBP-Type Peptidyl-Prolylcis-Trans Isomerase 17-2 and Is Involved in the Intracellular Movement of the Viral Coat Protein by Zuokun Yang, Yongle Zhang, Guoping Wang, Shaohua Wen, Yanxiang Wang, Liu Li, Feng Xiao, Ni Hong

“…Subcellular localization tests showed that the viral p23 translocated FKBP17-2 from chloroplasts to the plasmodesmata of epidermal cell<i>s</i> of <i>N. benthamiana</i> leaves. The knocked-down expression level of NbFKBP17-2 mRNA resulted in a decreased CTV titer in <i>N. benthamiana</i> plants. …”
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In Vivo Follow-Up of Gene Inhibition in Solid Tumors Using Peptide-Based Nanoparticles for siRNA Delivery by Isabel Ferreiro, Coralie Genevois, Karidia Konate, Eric Vivès, Prisca Boisguérin, Sébastien Deshayes, Franck Couillaud

“…In vitro and in vivo results showed efficient knock-down of Fluc expression with no toxicity. WRAP5:siFluc remained in the tumor for at least 10 days in vivo. …”
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Research on the Release of Dangerous Compounds from the BTEX and PAHs Groups in Industrial Casting Conditions by Mariusz Holtzer, Rafał Dańko, Sylwester Piasny, Michał Kubecki, Dariusz Drożyński, Agnieszka Roczniak, Mateusz Skrzyński, Angelika Kmita

“…The composition of gases that evolved during the individual stages, pouring, cooling and knocking out, was tested each time, and the contents of Polycyclic Aromatic Hydrocarbons (PAHs) and benzene, toluene, ethylbenzene, and xylenes (BETX) were analysed. …”
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<i>Porphyromonas gingivalis</i> Lipopolysaccharides Promote Proliferation and Migration of Human Vascular Smooth Muscle Cells through the MAPK/TLR4 Pathway by Megumi Miyabe, Nobuhisa Nakamura, Tomokazu Saiki, Satoru Miyabe, Mizuho Ito, Sachiko Sasajima, Tomomi Minato, Tatsuaki Matsubara, Keiko Naruse

“…To elucidate intracellular signaling, toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88) of HASMCs were knocked down, and the role of these molecules in <i>Pg</i>-LPS-stimulated proliferation and migration was examined. …”
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Mycobacterium tuberculosis Methyltransferase Rv1515c Can Suppress Host Defense Mechanisms by Modulating Immune Functions Utilizing a Multipronged Mechanism by Anshu Rani, Anshu Rani, Anwar Alam, Faraz Ahmad, Manjunath P., Abhinav Saurabh, Sheeba Zarin, Dipendra Kumar Mitra, Seyed E. Hasnain, Seyed E. Hasnain, Nasreen Z. Ehtesham

“…The DNA binding and DNA methyltransferase activity of Rv1515c was confirmed in vitro. Knock-in of Rv1515c in a model mycobacteria M. smegmatis (M. s_Rv1515c) resulted in remarkable physiological and morphological changes and conferred the recombinant strain with an ability to adapt to various stress conditions, including resistance to TB drugs. …”
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4-Octyl itaconate inhibits poly(I:C)-induced interferon-β secretion in mouse bone marrow-derived macrophages partially by activating Nrf2 by Ying-Xing Yue, Bing-Bing Jia, Ji-Rong Wang, Ying-Zheng Weng, Gen-Xiang Mao, Yan Lu, Jing Yan, Zhou-Xin Yang

“…The Nrf2 expression was knocked down with siRNA.4-OI inhibited poly(I:C)-induced IFN-β secretion and mRNA expression in BMDM. …”
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Insights into P-Glycoprotein Inhibitors: New Inducers of Immunogenic Cell Death by Joanna Kopecka, Martina Godel, Silvia Dei, Roberta Giampietro, Dimas Carolina Belisario, Muhlis Akman, Marialessandra Contino, Elisabetta Teodori, Chiara Riganti

“…Although Tariquidar did not alter the amount of Pgp present on cell surface, <i>R</i>-3 promoted Pgp internalization and ubiquitination, disrupting its interaction with CRT. Pgp knock-out restores doxorubicin-induced ICD in MDA-MB-231/DX cells that recapitulated the phenotype of <i>R</i>-3-treated cells. …”
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Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis by Lihua Dong, Jingjing Huang, Peng Zu, Jing Liu, Xue Gao, Jianwei Du, Yufu Li

“…When miR-101 was overexpressed, the results were the same as when TCF3 was knocked down. Moreover, we used Co-immunoprecipitation (Co-IP) to detect the interaction between TCF3 and HDAC3, and performed the Chromatin immunoprecipitation (ChIP) experiment to detect the enrichment of TCF3 and HDAC3 in the promoter region of miR-101. …”
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Modulation of Inflammatory Cytokine Production in Human Monocytes by cGMP and IRAK3 by Trang H. Nguyen, Anna Axell, Ilona Turek, Bree Wright, Terri Meehan-Andrews, Helen R. Irving

“…Downregulating cellular cGMP using a soluble GC inhibitor increased cytokine levels. Knocking down IRAK3 in THP-1 cells revealed that unlike the wild type cells, 8-Br-cGMP did not suppress inflammatory responses. …”
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Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin by Long Wang, Ting Zhang, Sha Liu, Qi Mo, Nan Jiang, Qi Chen, Jing Yang, Yun-Wei Han, Jian-Ping Chen, Fei-Hong Huang, Hua Li, Jie Zhou, Jie-Si Luo, Jian-Ming Wu

“…Furthermore, a RIT mice model and c-MPL knock-out (c-MPL-/-) mice constructed by CRISPR/Cas9 technology were used to assess the therapeutic action of ingenol on thrombocytopenia. …”
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Deletion of the primase-polymerases encoding gene, located in a mobile element in Thermus thermophilus HB27, leads to loss of function mutation of addAB genes by Carlos Verdú, Patricia Pérez-Arnaiz, Ana Peropadre, José Berenguer, Mario Mencía

“…Using different strategies we ablated the function of Ppol in HB27 cells, either by knocking out the gene through insertional mutagenesis, markerless deletion or through abolition of its catalytic activity. …”
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Cathepsin B-activatable cyclic antisense oligonucleotides for cell-specific target gene knockdown in vitro and in vivo by Zhongyu Wang, Xinli Fan, Guanqun Mu, Xiaoran Zhao, Qian Wang, Jing Wang, Xinjing Tang

“…CB-activatable cASOs were used to successfully knock down two disease-related endogenous genes in CB-abundant PC-3 tumor cells at the mRNA and protein levels but had much less effect on gene knockdown in CB-deficient human umbilical vein endothelial cell (HUVECs). …”
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CircNFIX stimulates the proliferation, invasion, and stemness properties of ovarian cancer cells by enhancing SH3RF3 mRNA stability via binding LIN28B by Xiu‐Zhang Yu, Bo‐Wen Yang, Meng‐Yin Ao, Yu‐Ke Wu, Hui Ye, Rui‐Yu Wang, Ming‐Rong Xi, Min‐Min Hou

“…CircNFIX and SH3RF3 were markedly elevated in OC tissues and OC cells. Knocking down circNFIX repressed the proliferation, migration, invasion, and stemness properties of A2780 and SKOV3 cells. …”
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Macrophage-Specific, <i>Mafb</i>-Deficient Mice Showed Delayed Skin Wound Healing by Yuri Inoue, Ching-Wei Liao, Yuki Tsunakawa, I-Lin Tsai, Satoru Takahashi, Michito Hamada

“…Comparison of the skin wound repair rates in macrophage-specific, MAFB-deficient mice (<i>Mafb^f/f</i>::LysM-Cre) and control mice (<i>Mafb^f/f</i>) showed that wound healing was significantly delayed in the former. For wounded GFP knock-in mice with GFP inserts in the <i>Mafb</i> locus, flow cytometry revealed that their GFP-positive cells expressed macrophage markers. …”
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Prognostic and therapeutic implications of iron-related cell death pathways in acute myeloid leukemia by Tongyu Li, Tongyu Li, Tongtong Lin, Jiahao Zhu, Miao Zhou, Miao Zhou, Shufang Fan, Hao Zhou, Hao Zhou, Qitian Mu, Qitian Mu, Lixia Sheng, Lixia Sheng, Guifang Ouyang, Guifang Ouyang

“…Subsequently, Western blot analysis showed that the expression levels of C-Myc and CyclinD1 were significantly reduced after CD4 expression levels were knocked down. The findings underscore the potential of iron-related cell death pathways as prognostic biomarkers and therapeutic targets in AML, paving the way for further research aimed at understanding the molecular mechanisms underlying the correlation between iron balance, apoptosis regulation, and immune modulation in the bone marrow microenvironment.…”
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Novel Latex Microsphere Immunochromatographic Assay for Rapid Detection of Cadmium Ion in Asparagus by Naifeng Xu, Qiaojuan Zhu, Jiangxiong Zhu, Jingze Jia, Xinlin Wei, Yuanfeng Wang

“…In this study, we innovatively combined molecular microbiology to carry out the comparative screening of Cd²⁺ chelators in a green, efficient, and specific way. The knock-out putative copper-transporter gene (<i>pca1</i>Δ) yeast strain with high sensitivity to Cd²⁺ was first used to screen the Cd²⁺ chelator, and the optimum chelator 1-(4-Isothiocyanatobenzyl)ethylenediamine-N,N,N,N′-tetraacetic acid (ITCBE) was obtained. …”
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LncRP11-675F6.3 responds to rapamycin treatment and reduces triglyceride accumulation via interacting with HK1 in hepatocytes by regulating... by Wang Lingling, Fang Xiaojuan, Yang Ziyou, Li Xueling, Cheng Mengdi, Cheng Liang, Wang Ganglin, Li Wei, Liu Lin

“…Furthermore, we find that ApoB100 is obviously colocalized with GFP-LC3 in autophagosomes when lncRP11-675F6. 3 is knocked down, indicating that elevated triglyceride accumulation likely related to autophagy induces the degradation of ApoB100 and impairs very low-density lipoprotein (VLDL) assembly. …”
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Triac Treatment Prevents Neurodevelopmental and Locomotor Impairments in Thyroid Hormone Transporter Mct8/Oatp1c1 Deficient Mice by Jiesi Chen, Eva Salveridou, Lutz Liebmann, Sivaraj M. Sundaram, Denica Doycheva, Boyka Markova, Christian A. Hübner, Anita Boelen, W. Edward Visser, Heike Heuer, Steffen Mayerl

“…Here, we directly compared their thyromimetic potential in Mct8/Oatp1c1 double knock-out mice (Dko) modeling human MCT8 deficiency. …”
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GAP-43 closely interacts with BDNF in hippocampal neurons and is associated with Alzheimer's disease progression by Ye Ji Lee, Ye Ji Jeong, Eun Ji Kang, Beom Seok Kang, Song Hee Lee, You Jin Kim, Seong Su Kang, Sang Won Suh, Eun Hee Ahn

“…In addition, we define the three-dimensional protein structure for GAP-43 and BDNF, including the predictive direct binding sites via analysis using ClusPro 2.0, and demonstrate that the deprivation of GAP-43 and BDNF triggers hippocampal neuronal death and memory dysfunction, employing the GAP-43 or BDNF knock-down cellular models and 5XFAD mice.ConclusionThese results show that GAP-43 and BDNF are direct binding partners in hippocampal neurons and that their molecular signaling might be potential therapeutic targets for AD.…”
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<i>Arabidopsis thaliana</i> G3BP Ortholog Rescues Mammalian Stress Granule Phenotype across Kingdoms by Hendrik Reuper, Benjamin Götte, Lucy Williams, Timothy J. C. Tan, Gerald M. McInerney, Marc D. Panas, Björn Krenz

“…Thus, members of the <i>Arabidopsis thaliana</i> G3BP (AtG3BP) protein family were investigated in a complementation assay in a human G3BP knock-out cell line. It was shown that two out of seven AtG3BPs were able to complement the function of their human homolog. …”
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