CRISPR Editing Enables Consequential Tag-Activated MicroRNA-Mediated Endogene Deactivation by Panayiota L. Papasavva, Petros Patsali, Constantinos C. Loucari, Ryo Kurita, Yukio Nakamura, Marina Kleanthous, Carsten W. Lederer

“…NHEJ-based tagging was imprecise and inefficient (≤6%) and uniformly produced knock-in- and indel-containing MRS tags, whereas HDR-based tagging was more efficient (≤18%), but toxic for longer donors encoding concatenated and thus potentially more efficient MRS tags. …”
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Long non‐coding RNA ESCCAL‐1/miR‐590/LRP6 signaling pathway participates in the progression of esophageal squamous cell carcinoma by Hongya Guan, Pengju Lv, Pengli Han, Lijuan Zhou, Jia Liu, Wei Wu, Ming Yan, Qinghe Xing, Wei Cao

“…Furthermore, ESCCAL‐1 was found to interact with miR‐590 and consequently restrict its expression. Functionally, knocking down ESCCAL‐1 or over‐expressing miR‐590 hindered ESCC cell growth, invasion, and migration in vitro. …”
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Innate antiviral responses against Shaan virus infection in HEK293, A549 and MARC-145 cells and limited role of viperin against Shaan virus replication by Hyun A. Lim, Ji Yeong Noh, Seong Sik Jang, Min Chan Kim, Soo Hyun Moon, Ha Yeon Kim, Da Young Mun, Hye Kwon Kim

“…Although viperin, an ISG, has broad inhibitory effects on a range of viral pathogens, viperin knockdown or knock-in in the infected cells indicated that this protein did not markedly affect Shaan virus replication. …”
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Gene-Edited Cell Models to Study Chronic Wasting Disease by Simrika Thapa, Cristobal Marrero Winkens, Waqas Tahir, Maria I. Arifin, Sabine Gilch, Hermann M. Schatzl

“…This is different for the few currently available knock-in mouse models that seem to do so. In this review, we discuss the available in vitro and in vivo models of CWD, and the impact of gene-editing strategies.…”
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Nrf2 Activates Augmenter of Liver Regeneration (ALR) via Antioxidant Response Element and Links Oxidative Stress to Liver Regeneration by Rania Dayoub, Arndt Vogel, Jutta Schuett, Madeleine Lupke, Susannah M. Spieker, Nadja Kettern, Eberhard Hildt, Michael Melter, Thomas S. Weiss

“…Performing partial hepatectomy in livers from Nrf2+/+ mice compared with Nrf2−/− knock-out (KO) mice, we found increased expression of ALR in addition to known antioxidant ARE-regulated genes. …”
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Bispecific antibody CAP256.J3LS targets V2-apex and CD4-binding sites with high breadth and potency by Baoshan Zhang, Jason Gorman, Young D. Kwon, Amarendra Pegu, Cara W. Chao, Tracy Liu, Mangaiarkarasi Asokan, Michael F. Bender, Tatsiana Bylund, Leland Damron, Deepika Gollapudi, Paula Lei, Yile Li, Cuiping Liu, Mark K. Louder, Krisha McKee, Adam S. Olia, Reda Rawi, Arne Schön, Shuishu Wang, Eun Sung Yang, Yongping Yang, Kevin Carlton, Nicole A. Doria-Rose, Lawrence Shapiro, Michael S. Seaman, John R. Mascola, Peter D. Kwong

“…The optimized bispecific antibody, which we named CAP256.J3LS, had a half-life similar to CAP256V2LS in human FcRn knock-in mice and exhibited suitable auto-reactivity, manufacturability, and biophysical risk. …”
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The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey by Kui Duan, Chen-Yang Si, Shu-Mei Zhao, Zong-Yong Ai, Bao-Hua Niu, Yu Yin, Li-Feng Xiang, Hao Ding, Yun Zheng

“…By single-cell RNA-sequencing (seq) data of cynomolgus monkeys, we found that LTRs of several ERV families, including MacERV6, MacERV3, MacERV2, MacERVK1, and MacERVK2, were highly expressed in pre-implantation embryo cells including epiblast (EPI), trophectoderm (TrB), and primitive endoderm (PrE), but were depleted in post-implantation. We knocked down MacERV6-LTR1a in cynomolgus monkeys with a short hairpin RNA (shRNA) strategy to examine the potential function of MacERV6-LTR1a in the early development of monkey embryos. …”
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Alternating Electric Fields (TTFields) Activate Cav1.2 Channels in Human Glioblastoma Cells by Eric Neuhaus, Lisa Zirjacks, Katrin Ganser, Lukas Klumpp, Uwe Schüler, Daniel Zips, Franziska Eckert, Stephan M. Huber

“…In addition, the expression of voltage-gated Ca2+ (Cav) channels was determined by real-time RT-PCR and their significance for the cellular TTFields response defined by knock-down and pharmacological blockade. As a result, TTFields stimulated in a cell line-dependent manner a Cav1.2-mediated Ca2+ entry, G1 or S phase cell cycle arrest, breakdown of the inner mitochondrial membrane potential and DNA degradation, and/or decline of clonogenic survival suggesting a tumoricidal action of TTFields. …”
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Developmental Inhibition of Long Intergenic Non-Coding RNA, <i>HOTAIRM1</i>, Impairs Dopamine Neuron Differentiation and Maturation by Xiaoying Cui, Renata Ap. Nedel Pertile, Zilong Du, Wei Wei, Zichun Sun, Darryl W. Eyles, James P. Kesby

“…To determine its role in early DA neuron development, we knocked down <i>HOTAIRM1</i> using RNAi in vitro in a human neuroblastoma cell line, and in vivo in mouse DA progenitors using a novel in utero electroporation technique. …”
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The Type III Effector AvrBs2 in Xanthomonas oryzae pv. oryzicola Suppresses Rice Immunity and Promotes Disease Development by Shuai Li, Yanping Wang, Shanzhi Wang, Anfei Fang, Jiyang Wang, Lijuan Liu, Kang Zhang, Yuling Mao, Wenxian Sun

“…Here, we examined the effect of the mutations of 23 putative nontranscription activator-like effector genes on X. oryzae pv. oryzicola virulence. The avrBs2 knock-out mutant was significantly attenuated in virulence to rice. …”
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Pescadillo ribosomal biogenesis factor 1 reduction suppresses tumour growth and renders chemosensitivity of head and neck squamous cell carcinoma by Dapeng Li, Changyu Yao, Zhao Ding, Ping Liu, Xue Chen, Weiwei Liu, Fangzheng Yi, Chuanya Jiang, Hongwu Li, Yehai Liu, Jing Wu

“…Methods A variety of bioinformatic approaches were used to identify the expressions, prognostic and diagnostic value of PES1 in HNSCC. qRT‐PCR, immunofluorescence (IF) assay, western blotting and immunohistochemical (IHC) were used to evaluate the expression of PES1 in HNSCC cell lines and clinical tissues. PES1 was knocked down in TU177 and FaDu cells which have high PES1 expression. …”
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A Tet-Inducible CRISPR Platform for High-Fidelity Editing of Human Pluripotent Stem Cells by Shawna L. Jurlina, Melissa K. Jones, Devansh Agarwal, Diana V. De La Toba, Netra Kambli, Fei Su, Heather M. Martin, Ryan Anderson, Ryan M. Wong, Justin Seid, Saisantosh V. Attaluri, Melissa Chow, Karl J. Wahlin

“…The potential of homology-directed repair (HDR) based gene knock-in at the <i>CLYBL</i> safe harbor site and endogenous <i>SOX2</i> and <i>SIX6</i> genes were demonstrated. …”
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Tumor PKCδ instigates immune exclusion in EGFR-mutated non–small cell lung cancer by Yi-Han Zuo, Wei-Na Gao, Ya-Jia Xie, Sheng-Yong Yang, Jin-Tai Zhou, Hai-Hai Liang, Xing-Xing Fan

“…CRISPR-Cas9 was used to knock out target genes. The changes of immune cells were monitored by flow cytometry. …”
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Experimental studies on premixed charge and reactivity-controlled compression ignition combustion modes using gasoline/diesel fuel combination by Ram Kishore Sankaralingam, Feroskhan M, Elango M, T.M. Yunus Khan, Syed Javed, Rahmath Ulla Baig, Venugopal Thangavel

“…However, this technology lacks combustion control to circumvent the knocking and misfire limitations. Premixed charge compression ignition (PCCI) and reactivity-controlled compression ignition (RCCI) are techniques that use partial homogeneous charge to reduce NOx and soot emissions. …”
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FGF21-FGFR1 controls mitochondrial homeostasis in cardiomyocytes by modulating the degradation of OPA1 by Bing Yan, Zhu Mei, Yaohan Tang, Haixu Song, Hanlin Wu, Quanmin Jing, Xiaolin Zhang, Chenghui Yan, Yaling Han

“…This study aimed to determine the mechanism underlying the cardioprotective effects of FGF21. We engineered FGF21 knock out mice and subsequently elucidated the effects of FGF21 and its downstream mediators using western blotting, qRT-PCR, and mitochondrial morphological and functional analyses. …”
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Pleiotropic Effects of the Protease-Activated Receptor 1 (PAR1) Inhibitor, Vorapaxar, on Atherosclerosis and Vascular Inflammation by Julian Friebel, Eileen Moritz, Marco Witkowski, Kai Jakobs, Elisabeth Strässler, Andrea Dörner, Daniel Steffens, Marianna Puccini, Stella Lammel, Rainer Glauben, Franziska Nowak, Nicolle Kränkel, Arash Haghikia, Verena Moos, Heinz-Peter Schutheiss, Stephan B. Felix, Ulf Landmesser, Bernhard H. Rauch, Ursula Rauch

“…In addition, vorapaxar reduced atherosclerotic lesion size in apolipoprotein E–knock out (ApoEko) mice. This reduction was associated with reduced expression of vascular adhesion molecules and TLR2/4 presence, both in isolated murine endothelial cells and the aorta. …”
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Targeted OUM1/PTPRZ1 silencing and synergetic CDT/enhanced chemical therapy toward uveal melanoma based on a dual-modal imaging-guided manganese metal–organic framework nanoparticl... by Yue Li, Fang Li, Hui Pan, Xiaolin Huang, Jie Yu, Xueru Liu, Qinghao Zhang, Caiwen Xiao, He Zhang, Leilei Zhang

“…Hence, siRNAs that selectively knocking down the lncRNA OUM1 (siOUM1) and its target gene PTPRZ1 (siPTPRZ1) were designed to inhibit the OUM1/PTPRZ1 pathway to reduce PTP activity, and this reduction in activity interrupts protein tyrosine phosphorylation, suppresses UM proliferation and metastasis and improves cisplatin sensitivity in UM cells. …”
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Gene Expression Profiling Reveals Fundamental Sex-Specific Differences in SIRT3-Mediated Redox and Metabolic Signaling in Mouse Embryonic Fibroblasts by Robert Belužić, Ena Šimunić, Iva I. Podgorski, Marija Pinterić, Marijana Popović Hadžija, Tihomir Balog, Sandra Sobočanec

“…Furthermore, the loss of Sirt-3 in male MEFs leads to mitochondrial and endoplasmic reticulum stress. Since Sirt-3 knock-out is permanent, male cells are forced to function in a state of persistent oxidative and metabolic stress. …”
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Interplay between bacterial 5′-NAD-RNA decapping hydrolase NudC and DEAD-box RNA helicase CsdA in stress responses by Milda Mickutė, Renatas Krasauskas, Kotryna Kvederavičiūtė, Gytė Tupikaitė, Aleksandr Osipenko, Algirdas Kaupinis, Monika Jazdauskaitė, Raminta Mineikaitė, Mindaugas Valius, Viktoras Masevičius, Giedrius Vilkaitis

“…Moreover, we confirmed the physical association of NudC with the cold shock DEAD-box RNA helicase CsdA and the RNA chaperone Hfq. Interestingly, knocking out csdA similar to ∆nudC leads to an increased number of identified 5′-NAD-RNA species compared to wild type, exposing CsdA as a new player in a potentially unexplored layer of NAD-epitranscriptomic landscape. …”
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Prognostic and therapeutic significance of a novel ferroptosis related signature in colorectal cancer patients by Songtao Du, Furong Zeng, Huiyan Sun, Yanlong Liu, Peng Han, Bomiao Zhang, Weinan Xue, Guangtong Deng, Mingzhu Yin, Binbin Cui

“…Interestingly, CRC cells with higher FRGs risk score were more sensitive to RSL3. Knocking down GSS and NFS1 increased the FRGs risk score and the sensitivity of CRC cells to RSL3. …”
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