2,5-Hexanedione Affects Ovarian Granulosa Cells in Swine by Regulating the <i>CDKN1A</i> Gene: A Transcriptome Analysis by Yige Chen, Chengcheng Kong, Min Yang, Yangguang Liu, Zheng Han, Liming Xu, Xianrui Zheng, Yueyun Ding, Zongjun Yin, Xiaodong Zhang

“…Thus, we evaluated its function in pGC apoptosis in vitro. Then, we knocked down the <i>CDKN1A</i> gene in the pGCs to identify its effects on pGCs. …”
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V-Set and Immunoglobulin Domain-Containing 1 (VSIG1), Predominantly Expressed in Testicular Germ Cells, Is Dispensable for Spermatogenesis and Male Fertility in Mice by Yena Jung, Hyewon Bang, Young-Hyun Kim, Na-Eun Park, Young-Ho Park, Chaeli Park, Sang-Rae Lee, Jeong-Woong Lee, Bong-Seok Song, Ji-Su Kim, Bo-Woong Sim, Dong-Won Seol, Gabbine Wee, Sunhyung Kim, Sun-Uk Kim, Ekyune Kim

“…To elucidate the functional role of V-set and immunoglobulin domain-containing 1 (VSIG1) in spermatogenesis and fertilization, we knocked out (KO) VSIG1 in a mouse embryo using CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) -mediated genome editing. …”
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Single intranasal immunization with chimpanzee adenovirus-based vaccine induces sustained and protective immunity against MERS-CoV infection by Wenxu Jia, Rudragouda Channappanavar, Chao Zhang, Mingxi Li, Haixia Zhou, Shuyuan Zhang, Panpan Zhou, Jiuyang Xu, Sisi Shan, Xuanling Shi, Xinquan Wang, Jincun Zhao, Dongming Zhou, Stanley Perlman, Linqi Zhang

“…Single intranasal immunization with AdC68-S induced robust and sustained neutralizing antibody and T cell responses in BALB/c mice. In a human DPP4 knock-in (hDPP4-KI) mouse model, it completely protected against lethal challenge with a mouse-adapted MERS-CoV (MERS-CoV-MA). …”
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Huntingtin Co-Isolates with Small Extracellular Vesicles from Blood Plasma of TgHD and KI-HD Pig Models of Huntington’s Disease and Human Blood Plasma by Hanadi Ananbeh, Jaromir Novak, Stefan Juhas, Jana Juhasova, Jiri Klempir, Kristyna Doleckova, Irena Rysankova, Karolina Turnovcova, Jaroslav Hanus, Hana Hansikova, Petr Vodicka, Helena Kupcova Skalnikova

“…We aimed to characterize HTT and mHTT forms/fragments in blood plasma derived EVs in transgenic (TgHD) and knock-in (KI-HD) porcine models, as well as in HD patients’ plasma. (2) Methods: Small EVs were isolated by ultracentrifugation and HTT forms were visualized by western blotting. (3) Results: The full length 360 kDa HTT co-isolated with EVs from both the pig model and HD patient plasma. …”
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Loss of miR-369 Promotes Tau Phosphorylation by Targeting the Fyn and Serine/Threonine-Protein Kinase 2 Signaling Pathways in Alzheimer’s Disease Mice by Xiaoguang Yao, Xiaoguang Yao, Xiaoguang Yao, Xiaohui Xian, Xiaohui Xian, Mingxing Fang, Mingxing Fang, Shujuan Fan, Shujuan Fan, Wenbin Li, Wenbin Li

“…Luciferase reporter assays were applied to confirm the predicted targets of miR-369.ResultsKnocking out miR-369 in 3xTg AD mice aggravated cognitive impairment, promoted hyperphosphorylation of tau, and upregulated Fyn and SRPK2. …”
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Hypothalamic TTF-1 orchestrates the sensitivity of leptin by Byong Seo Park, Dasol Kang, Kwang Kon Kim, Bora Jeong, Tae Hwan Lee, Jeong Woo Park, Shioko Kimura, Jung-Yong Yeh, Gu Seob Roh, Chang-Joong Lee, Sungchil Yang, Sunggu Yang, Jae Geun Kim, Byung Ju Lee

“…Methods: We generated conditional knock-out mice by crossing TTF-1flox/flox mice with leptin receptor (ObRb)Cre or proopiomelanocortin (POMC)Cre transgenic mice to interrogate the contributions of TTF-1 in leptin signaling and activity. …”
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Crop improvements for future‐proofing European food systems: A focus‐group‐driven analysis of agricultural production stakeholder priorities and viewpoints by Stacia Stetkiewicz, Jonathan Menary, Abhishek Nair, Mariana C. Rufino, Arnout R.H. Fischer, Marc Cornelissen, Remi Duchesne, Adrien Guichaoua, Petra Jorasch, Stephane Lemarié, Amrit K. Nanda, Ralf Wilhelm, Jessica A.C. Davies

“…Understanding broader agri‐food system knock‐on effects of plant innovation is a non‐trivial challenge requiring interdisciplinary research and close partnerships with food system stakeholders.…”
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Mechanism of Interaction between hsa_circ_0002854 and MAPK1 Protein in PM_2.5-Induced Apoptosis of Human Bronchial Epithelial Cells by Jinchang Hong, Yi Tan, Yuyu Wang, Hongjie Wang, Caixia Li, Wenjia Jin, Yi Wu, Dechun Ni, Xiaowu Peng

“…Functional analysis showed that knocking down the expression of hsa_circ_0002854 could inhibit apoptosis induced by PM_2.5 exposure. …”
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ERα36–GPER1 Collaboration Inhibits TLR4/NFκB-Induced Pro-Inflammatory Activity in Breast Cancer Cells by George Notas, Athanasios Panagiotopoulos, Rodanthi Vamvoukaki, Konstantina Kalyvianaki, Foteini Kiagiadaki, Alexandra Deli, Marilena Kampa, Elias Castanas

“…In the presence of ERα66, ERα36 or GPER1 knock-down partially inhibits NFκB-mediated inflammation. …”
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Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy by Yang Deng, Rui Duan, Wangli Ding, Qiuchen Gu, Manman Liu, Junshan Zhou, Jianguo Sun, Junrong Zhu

“…These effects were reversed when astrocytes were pretreated with GW4869, an exosome secretion inhibitor, or when hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) was knocked down. Neuroprotection was also observed during treatment with OGD/R-ADEXs in vivo. …”
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Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas by Yinan Jiang, John Wiersch, Wei Wu, Wei Wu, Jieqi Qian, Jieqi Qian, Maharana Prathap R. Adama, Nannan Wu, Nannan Wu, Weixia Yang, Weixia Yang, Congde Chen, Congde Chen, Lingyan Zhu, Krishna Prasadan, George K. Gittes, Xiangwei Xiao

“…A ROSA-mTmG red fluorescent mouse was surgically joined to an INS1Cre knock-in mouse by parabiosis to establish a mixed circulation. …”
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LIS1, a glyco-humanized swine polyclonal anti-lymphocyte globulin, as a novel induction treatment in solid organ transplantation by Juliette Rousse, Pierre-Joseph Royer, Gwénaëlle Evanno, Elsa Lheriteau, Carine Ciron, Apolline Salama, Françoise Shneiker, Roberto Duchi, Andrea Perota, Cesare Galli, Emmanuele Cozzi, Gilles Blancho, Odile Duvaux, Sophie Brouard, Jean-Paul Soulillou, Jean-Marie Bach, Bernard Vanhove

“…We investigated here the in vitro and in vivo activity of LIS1, a glyco-humanized ALG (GH-ALG) produced in pigs knocked out for the two major xeno-antigens αGal and Neu5Gc. …”
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Deletion of the Actin-Associated Tropomyosin <i>Tpm3</i> Leads to Reduced Cell Complexity in Cultured Hippocampal Neurons—New Insights into the Role of the C-Terminal Region of Tpm... by Tamara Tomanić, Claire Martin, Holly Stefen, Esmeralda Parić, Peter Gunning, Thomas Fath

“…Here, we used primary mouse hippocampal neurons of C57/Bl6 wild type and Bl6^Tpm3flox transgenic mice to carry out morphometric analyses in response to the absence of <i>Tpm3</i> products, as well as to investigate the effect of C-terminal truncation on the ability of Tpm3.1 to modulate neuronal morphogenesis. We found that the knock-out of <i>Tpm3</i> leads to decreased neurite length and complexity, and that the deletion of two amino acid residues at the C-terminus of Tpm3.1 leads to more detrimental changes in neurite morphology than the deletion of six amino acid residues. …”
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Mefenamic Acid-Upregulated Nrf2/SQSTM1 Protects Hepatocytes against Oxidative Stress-Induced Cell Damage by Wonseok Lee, Yewon Mun, Kang-Yo Lee, Jung-Min Park, Tong-Shin Chang, You-Jin Choi, Byung-Hoon Lee

“…This cytoprotective effect was abolished when SQSTM1 was knocked down, suggesting the involvement of SQSTM1 in mediating the protective effect of MFA against tBHP-induced toxicity. …”
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Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress by Samina Shabbir, Weiting Wang, Mohsin Nawaz, Prerona Boruah, Muhammad Fakhar-e-Alam Kulyar, Mao Chen, Bo Wu, Panting Liu, Yonghua Dai, Lingling Sun, Qiyu Gou, Renbin Liu, Guoquan Hu, Tahira Younis, Mingxiong He

“…Based on RNA-Seq data, two differentially expressed genes, ZMO_RS02740 (up-regulated) and ZMO_RS06525 (down-regulated) were knocked out and over-expressed through CRISPR-Cas technology to investigate their roles in AF tolerance. …”
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Effect of Dorper Rams’ Social-Sexual Hierarchy on Their Sexual Behavior and Capacity to Induce Estrus in Ewes by Andrea González-Tavizón, César A. Meza-Herrera, Gerardo Arellano-Rodríguez, Miguel Mellado, Viridiana Contreras-Villarreal, Oscar Ángel-García, José R. Arévalo, Francisco G. Véliz-Deras

“…Regarding aggressive behaviors, HHR showed a higher <i>(p</i> < 0.05) proportion of rams showing threatening, knocking, and blocking behaviors than LHR. Appetitive behavior was higher <i>(p</i> < 0.05) in HHR than LHR rams (3576 ± 0.7 vs. 3054 ± 0.7 number of events). …”
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CRISPR/Cas9-Induced Knockout of miR-24 Reduces Cholesterol and Monounsaturated Fatty Acid Content in Primary Goat Mammary Epithelial Cells by Lian Huang, Jun Luo, Wenchang Gao, Ning Song, Huibin Tian, Lu Zhu, Qianming Jiang, Juan J. Loor

“…Similarly, the content of cholesterol and monounsaturated fatty acid (MUFA) C18:1 decreased, whereas that of polyunsaturated fatty acids (PUFA) C18:2, C20:3, C20:4 and C20:5 increased. Subsequently, knocking down of <i>INSIG1</i> but not <i>FASN</i> reversed the effect of miR-24 knockout, indicating that miR-24 modulated cholesterol and fatty acid synthesis mainly by targeting <i>INSIG1</i>. …”
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A novel recombination protein C12ORF40/REDIC1 is required for meiotic crossover formation by Suixing Fan, Yuewen Wang, Hanwei Jiang, Xiaohua Jiang, Jianteng Zhou, Yuying Jiao, Jingwei Ye, Zishuo Xu, Yue Wang, Xuefeng Xie, Huan Zhang, Yang Li, Wei Liu, Xiangjun Zhang, Hui Ma, Baolu Shi, Yuanwei Zhang, Muhammad Zubair, Wasim Shah, Zhipeng Xu, Bo Xu, Qinghua Shi

“…Spread mouse spermatocyte fluorescence immunostaining showed that REDIC1 forms discrete foci between the paired regions of homologous chromosomes depending on strand invasion and colocalizes with MSH4 and later with MLH1 at the crossover sites. Redic1 knock-in (KI) mice homozygous for mutation c.232_233insTT are infertile in both sexes due to insufficient crossovers and consequent meiotic arrest, which is also observed in our patients. …”
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Pien-tze-huang promotes wound healing in streptozotocin-induced diabetes models associated with improving oxidative stress via the Nrf2/ARE pathway by Ying Liu, Jiake Mo, Fang Liang, Siwei Jiang, Jing Xiong, Xubiao Meng, Zhaohui Mo

“…In vitro assays showed that PZH improved the proliferation, migration and angiogenic function of human umbilical vein endothelial cells (HUVECs) cultured in palmitic acid, reduced the expression of the apoptotic proteins p53, Bax, and cleaved-caspase3, and activated Nrf2/ARE signaling; however, these protective effects were abrogated after Nrf2 was knocked down by specific siRNA. In addition, the levels of the serum inflammatory cytokines IL-1β, TNF-α, and IL-6 were reduced after PZH gavage treatment. …”
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Chronic stress from adolescence to adulthood increases adiposity and anxiety in rats with decreased expression of Krtcap3 by Alexandria M. Szalanczy, Mackenzie Fitzpatrick, Angela Beeson, Trangdai Bui, Christina Dyson, Seth Eller, Julia Landry, Christina Scott, Michael Grzybowski, Jason Klotz, Aron M. Geurts, Jeff L. Weiner, Eva E. Redei, Leah C. Solberg Woods

“…To more thoroughly understand the connection between Krtcap3, adiposity, and stress, we exposed wild-type (WT) and Krtcap3 knock-out (KO) rats to chronic stress then measured adiposity and behavioral outcomes. …”
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