Functional analysis of SH3 domain containing ring finger 2 during the myogenic differentiation of quail myoblast cells by Si Won Kim, Jeong Hyo Lee, Tae Sub Park

“…Subsequently, SH3RF2 was knocked out using clustered regularly interspaced short palindromic repeat/Cas9 technology and single cell-derived SH3RF2 mutant sublines were established to study the biofunctional activity of SH3RF2 in quail myoblast (QM7) cells during muscle differentiation. …”
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Stimulation of protein synthesis by optogenetic and chemical induction of excitatory synaptic plasticity in hippocampal somatostatin interneurons by Ève Honoré, Inês Belo do Nascimento, Isabel Laplante, Jean-Claude Lacaille

“…Moreover, protein synthesis stimulated by persistent LTP induction was prevented in mice with a SOM-IN conditional knock-out of Raptor, an essential component of mTORC1, indicating a critical role of mTORC1 in the control of translation in PC-SOM synapse plasticity. …”
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Detection and isolation of wormhole nodes in wireless ad hoc networks based on post-wormhole actions by David Samuel Bhatti, Shahzad Saleem, Azhar Imran, Hyeon Jeong Kim, Ki-Il Kim, Kyu-Chul Lee

“…In terms of contributions, the proposed solution addresses the knock-out capability of attackers that is less targeted by the researcher’s community. …”
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Pharmacophore-Aided Virtual Screening and Molecular Dynamics Simulation Identifies TrkB Agonists for Treatment of -Deficiency Disorders by Ibitayo Abigail Ademuwagun, Gbolahan Oladipupo Oduselu, Solomon Oladapo Rotimi, Ezekiel Adebiyi

“…We hereby recommend experimental validation of the best hits in CDKL5 knock out models before consideration as drugs in CDD management.…”
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Loss of <i>MXRA8</i> Delays Mammary Tumor Development and Impairs Metastasis by Kaitlyn E. Simpson, Christina A. Staikos, Katrina L. Watson, Roger A. Moorehead

“…Therefore, the aim of this research was to determine the role of <i>MXRA8</i> in breast cancer by knocking out <i>MXRA8</i> in the human triple-negative breast cancer cell line MDA-MB-231. …”
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RPL41, a Small Ribosomal Peptide Deregulated in Tumors, Is Essential for Mitosis and Centrosome Integrity by Shan Wang, Jianmin Huang, Jie He, Aiyuan Wang, Shengqiang Xu, Shiu-Feng Huang, Sheng Xiao

“…Microtubule spindles are essential for chromosome segregation during mitosis. Cells with RPL41 knock-down showed abnormal spindles, frequent failure of cytokinesis, and formation of polynuclear cells. …”
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The <it>Zymomonas mobilis </it>regulator <it>hfq </it>contributes to tolerance against multiple lignocellulosic pretreatment inhibitors by Lu Tse-Yuan S, Pelletier Dale A, Yang Shihui, Brown Steven D

“…</p> <p>Conclusions</p> <p>We have shown the utility of the pKNOCK suicide plasmid for mutant construction in <it>Z. mobilis</it>, and constructed a Gateway compatible expression plasmid for use in <it>Z. mobilis </it>for the first time. …”
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PS1 Affects the Pathology of Alzheimer’s Disease by Regulating BACE1 Distribution in the ER and BACE1 Maturation in the Golgi Apparatus by Nuomin Li, Yunjie Qiu, Hao Wang, Juan Zhao, Hong Qing

“…Previous studies have shown that <i>PSEN1</i> mutations increase the expression and activity of BACE1 and that BACE1 expression and activity are elevated in the brains of <i>PSEN1</i> mutant knock-in mice, compared with wild-type mice, as well as in the cerebral cortex of FAD patients carrying <i>PSEN1</i> mutations, compared with sporadic AD patients and controls. …”
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Effects of KLK Peptide on Adjuvanticity of Different ODN Sequences by Ghania Chikh, Rachel Luu, Shobhna Patel, Heather L. Davis, Risini D. Weeratna

“…The synergistic adjuvant effects with KLK/ODN combinations were TLR9-mediated since they did not occur in TLR9 knock-out mice. We hypothesize that a nuclease resistant ODN with CpG motifs has its own mechanism for entering cells to reach the endosome. …”
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Abnormalities in the SIRT1-SIRT3 axis promote myocardial ischemia-reperfusion injury through ferroptosis caused by silencing the PINK1/Parkin signaling pathway by Yunfei Liao, Ben Ke, Xiaoyan Long, Jianjun Xu, Yongbing Wu

“…Then, Pearson correlation analysis was further used to explore the relationship between SIRT1 and SIRT3, whose roles in the regulation of ferroptosis were also analysed by gene knock down, Western Blotting and flow cytometry. …”
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The upregulation of lamin A/C as a compensatory mechanism during tight junction disruption in renal tubular cells mediated by calcium oxalate crystals by Sudarat Hadpech, Paleerath Peerapen, Visith Thongboonkerd

“…While roles for F-actin and α-tubulin and their association with ZO-1 are known to regulate COM-mediated tight junction (TJ) disruption, roles of lamin A/C and its interplay with ZO-1 in COM kidney stone model remain unclear and are thus the objectives of this study. Lamin A/C was knocked down in MDCK cells by silencing RNA specific for LMNA (siLMNA). …”
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MicroRNA-989 targets 5-hydroxytryptamine receptor1 to regulate ovarian development and eggs production in Culex pipiens pallens by Junnan Zheng, Jingwei Xu, Ruiming Zhang, Jiajia Du, Huan Wang, Jinze Li, Dan Zhou, Yan Sun, Bo Shen

“…Methods The spatiotemporal expression profile of miR-989 was detected using quantitative real-time reverse transcription PCR (RT-qPCR). miR-989 antagomirs and antagomir-negative control (NC) were designed and synthesized to knock down the expression of endogenous miR-989 in female mosquitoes. …”
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Filtration and tubular handling of EWE‐hC3Nb1, a complement inhibitor nanobody, in wild type mice and a mouse model of proteinuric kidney disease by Morten Schøler Fast, Kathrin Weyer, Henrik Pedersen, Gregers Rom Andersen, Henrik Birn

“…Here, we examined the pharmacokinetic properties of free EWE‐hC3Nb1 in mouse plasma and urine, following subcutaneous injection in wild‐type control and podocin knock out (KO) mice with severe proteinuria. Tubular handling of filtered EWE‐hC3Nb1 was assessed by immunohistochemistry (IHC) on kidney tissue from control, proteinuric mice, and KO mice deficient in the proximal tubule endocytic receptor megalin. …”
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Nucleophosmin 1 associating with engulfment and cell motility protein 1 regulates hepatocellular carcinoma cell chemotaxis and metastasis by Yang Gangqi, Li Hongyan, Dong Zheng, Deng Kai, Lu Yinying

“…In this study, a small interfering RNA technique was used to knock down the Nucleophosmin 1 (NPM1) gene expression. …”
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Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid by Dexin Wang, Xiaoping Fu, Dasen Zhou, Jiaqi Gao, Wenqin Bai

“…Second, the sucA, gudB, pgdS, and ggt genes were knocked out simultaneously, which increased the titer of γ-PGA by 1.75 times. …”
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Lipopolysaccharide Inhibits Autophagy and Promotes Inflammatory Responses via p38 MAPK-Induced Proteasomal Degradation of Atg13 in Hepatic Stellate Cells by Yuchao Wu, Yajuan He, Fei Wang, Naijuan Yao, Yingren Zhao, Zhen Tian

“…Secretion of IL-1β was assessed by ELISA. Atg13 was knocked down by siRNA in LX2 cells. Coimmunoprecipitation assay was used to detect protein binding and polyubiquitination of Atg13. …”
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Deciphering Microglia-Synapse Interaction in Neuroligin-4 Knockout mouse model of Autism Spectrum Disorder by Bilge Ugursu, Helmut Kettenmann, Susanne A. Wolf

“…Accumulating evidence, mainly derived from genetic studies revealing Neuroligin mutations in autism spectrum disorders (ASD), suggests synaptopathy as an underlying reason of the disease; however, comprehensive studies characterizing microglia-synapse interaction in ASD models are lacking.Herein, we investigated interaction of microglia and synapses in a Neuroligin-4 knock-out (NL4-KO) mouse model of ASD along with the main pathways involved in their cross-talk. …”
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De novo emergence, existence, and demise of a protein-coding gene in murids by Jan Petrzilek, Josef Pasulka, Radek Malik, Filip Horvat, Shubhangini Kataruka, Helena Fulka, Petr Svoboda

“…These events generated a cytoplasmic serine-rich maternal protein. Knock-out of D6Ertd527e in mice has a small but detectable effect on fertility and the maternal transcriptome. …”
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In Vitro Differentiation of Preosteoblast-Like Cells, MC3T3-E1, to Adipocytes Is Enhanced by 1,25(OH)2 Vitamin D3 by Elisha Pendleton, Nalini Chandar

“…To determine how the effect of vitamin D might compare to other genetic manipulations that promote adipogenic differentiation, we stably knocked down retinoblastoma expression in MC3T3-E1 cells. …”
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Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells. by Jillian A Bristol, Joshua Brand, Makoto Ohashi, Mark R Eichelberg, Alejandro Casco, Scott E Nelson, Mitchell Hayes, James C Romero-Masters, Dana C Baiu, Jenny E Gumperz, Eric C Johannsen, Huy Q Dinh, Shannon C Kenney

“…In addition to its essential role in plasma cell differentiation, IRF4 decreases BCR signaling. Knock-down of IRF4 in a T1 LCL (infected with the Zp-V3-containing Akata strain) induced lytic reactivation whereas over-expression of IRF4 in Burkitt lymphoma cells inhibited both NFATc1 and NFATc2 expression and lytic EBV reactivation. …”
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