Corosolic Acid Attenuates the Invasiveness of Glioblastoma Cells by Promoting CHIP-Mediated AXL Degradation and Inhibiting GAS6/AXL/JAK Axis by Li-Wei Sun, Shao-Hsuan Kao, Shun-Fa Yang, Shang-Wun Jhang, Yi-Chen Lin, Chien-Min Chen, Yi-Hsien Hsieh

“…Further investigation revealed that CA decreased AXL level by promoting ubiquitin-mediated proteasome degradation and upregulating the carboxyl terminus of Hsc70-interacting protein (CHIP), an inducer of AXL polyubiquitination. CHIP knock-down restored the CA-reduced AXL and invasiveness of GBM cells. …”
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p53 status modifies cytotoxic activity of lactoferrin under hypoxic conditions by Maryami Yuliana Kosim, Takahiro Fukazawa, Mutsumi Miyauchi, Nobuyuki Hirohashi, Keiji Tanimoto

“…MTT assay strikingly demonstrated that cells expressing mutant-type p53 (MT5) were more sensitive to LF than control HepG2 (hepatoma), suggesting an important role of the p53 signal. Knock-down of TP53 (p53 gene) interestingly reduced sensitivity to LF in HepG2, suggesting that p53 may be a target of LF cytotoxic activity. …”
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Biological conversion of methane to putrescine using genome-scale model-guided metabolic engineering of a methanotrophic bacterium Methylomicrobium alcaliphilum 20Z by Linh Thanh Nguyen, Eun Yeol Lee

“…For elimination of the putrescine utilization pathway, spermidine synthase (MEALZ_3408) was knocked out, resulting in no spermidine formation in the strain 20ZES1-pACO with a putrescine titer of 18.43 mg/L. …”
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The Tatar-Russian Bilingualism in Early Childhood by Hristo Kyuchukov, Oksana S. Ushakova, Farida S. Gazizova

“…They were also tested with a psychological non-verbal Knock Tap test. All children were tested individually in a separate room by a native Tatar speaking and Russian speaking researcher. …”
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Ubiquitin‐specific protease 11‐mediated CD36 deubiquitination acts on C1q/TNF‐related protein 9 against atherosclerosis by Min Zeng, Xin Wei, Yangli He, Yali Yang

“…USP11 knockdown decreased the CD36 protein expression and pre‐treatment with 10 μg/mL MG132 significantly maintained the CD36 level from USP11 knock down. Up‐regulation of CD36 reversed the alterations on the cholesterol metabolism caused by CTRP9 or USP11 knockdown. …”
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SIPA1 Is a Modulator of HGF/MET Induced Tumour Metastasis via the Regulation of Tight Junction-Based Cell to Cell Barrier Function by Chang Liu, Wenguo Jiang, Lijian Zhang, Rachel Hargest, Tracey A. Martin

“…In vitro cell function assays were performed after knock down of SIPA1 in lung cancer cells with/without treatment. …”
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Trans-(−)-Kusunokinin: A Potential Anticancer Lignan Compound against HER2 in Breast Cancer Cell Lines? by Thidarath Rattanaburee, Tanotnon Tanawattanasuntorn, Tienthong Thongpanchang, Varomyalin Tipmanee, Potchanapond Graidist

“…Meanwhile, neratinib downregulated HER, MEK1, ERK, c-Myc, CyclinB1, CyclinD and CDK1. Knocking down HER2 downregulated only HER2. siRNA-HER2 combination with (±)-kusunokinin suppressed HER2, c-Myc, CyclinB1, CyclinD and CDK1. …”
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Exploring psilocybin-assisted psychotherapy in the treatment of methamphetamine use disorder by Jonathan Brett, Jonathan Brett, Elizabeth Knock, P. Todd Korthuis, Paul Liknaitzky, Paul Liknaitzky, Kevin S. Murnane, Kevin S. Murnane, Christopher R. Nicholas, James C. Patterson, James C. Patterson, Christopher S. Stauffer, Christopher S. Stauffer

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GPR17: Molecular modeling and dynamics studies of the 3-D structure and purinergic ligand binding features in comparison with P2Y receptors by Ranghino Graziella, Abbracchio Maria P, Parravicini Chiara, Fantucci Piercarlo

“…In a rat brain focal ischemia model, the selective <it>in vivo </it>knock down of GPR17 by anti-sense technology or P2Y/CysLT antagonists reduced progression of ischemic damage, thus highlighting GPR17 as a novel therapeutic target for stroke. …”
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Endothelial NOX5 Expression Modulates Thermogenesis and Lipolysis in Mice Fed with a High-Fat Diet and 3T3-L1 Adipocytes through an Interleukin-6 Dependent Mechanism by Jorge G. García, Carlos de Miguel, Fermín I. Milagro, Guillermo Zalba, Eduardo Ansorena

“…An in vivo model based on NOX5 conditional knock-in mice fed with a high-fat diet and an in vitro model developed with 3T3-L1 adipocytes cultured with conditioned media of endothelial NOX5-expressing bEnd.3 cells, previously treated with glucose and palmitic acid. …”
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Tissue‐Specific Roles for the Slit–Robo Pathway During Heart, Caval Vein, and Diaphragm Development by Juanjuan Zhao, Susann Bruche, Helen G. Potts, Benjamin Davies, Mathilda T. M. Mommersteeg

“…Neural crest‐specific removal of Robo2 in Robo1 knockouts showed fully penetrant bicuspid aortic valves (BAV). Endocardial knock‐out of either Slit2or Robo4 caused low penetrant BAV. …”
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A new mouse model of Charcot-Marie-Tooth 2J neuropathy replicates human axonopathy and suggest alteration in axo-glia communication. by Ghjuvan'Ghjacumu Shackleford, Leandro N Marziali, Yo Sasaki, Anke Claessens, Cinzia Ferri, Nadav I Weinstock, Alexander M Rossor, Nicholas J Silvestri, Emma R Wilson, Edward Hurley, Grahame J Kidd, Senthilvelan Manohar, Dalian Ding, Richard J Salvi, M Laura Feltri, Maurizio D'Antonio, Lawrence Wrabetz

“…To study this, we generated targeted knock-in MpzT124M mutant mice, a genetically authentic model of T124M-CMT2J neuropathy. …”
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Dioscin alleviates hashimoto’s thyroiditis by regulating the SUMOylation of IRF4 to promote CD4+CD25+Foxp3+ treg cell differentiation by Cao Yongjun, Qiao Nan, Sun Yumeng, Jin Xiaowen, Wen Weibo

“…Furthermore, the SUMOylation of IRF4 was increased after SENP1 was knocked down. The results of our study indicate that dioscin can promote the differentiation of the CD4+CD25+Foxp3+ Treg cells and subsequently upregulate the SUMOylation of IRF4 by downregulating SENP1 expression.…”
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Characterization of DoTc2 4510—Identifying HPV16 Presence in a Cervical Carcinoma Cell Line Previously Considered to Be HPV-Negative by Nika Vučković, Karin Hoppe-Seyler, Angelika B. Riemer

“…Finally, to attest to the survival dependence of DoTc2 4510 cells on HPV16, we performed an HPV16 E6/E7-targeted siRNA knock-down, which indeed led to senescence induction. …”
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Why is Uptake of the Disability Tax Credit Low in Canada? Exploring Possible Barriers to Access by Stephanie Dunn, Jennifer Zwicker

“…Creation of a clear and transparent review and appeals process to replace the current and often inconsistent one would further knock down barriers to access. Improving the disability assessment process for the DTC and for other key disability benefits is also essential. …”
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Dislocation Loop Generation Differences between Thin Films and Bulk in EFDA Pure Iron under Self-Ion Irradiation at 20 MeV by Marcelo Roldán, Fernando José Sánchez, Pilar Fernández, Christophe J. Ortiz, Adrián Gómez-Herrero, David Jiménez Rey

“…Apparent differences were found between the thin foil irradiation and the first 100 nm in bulk specimens in terms of dislocation loops, even with a similar primary knock-on atom (PKA) spectrum. In thin films, the most loops identified in all four experimental conditions were b ±a₀<100>{200} type with sizes of hundreds of nm depending on the experimental conditions, similarly to bulk samples where practically no defects were detected. …”
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Heat shock protein 40 of Streptococcus pneumoniae induces immune response of human dendritic cells via TLR4‐dependent p38 MAPK and JNK signaling pathways by Jing‐jing Liu, Jian‐cheng Lin, Li‐na Chen

“…Short hairpin RNAs were used to knock down toll‐like receptor 2 (TLR2) and TLR4. …”
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Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli by Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang, Qingsheng Qi

“…To prevent the preferable conversion of cinnamaldehyde to cinnamyl alcohol in wild-type Escherichia coli, the E. coli MG1655 strain with reduced aromatic aldehyde reduction (RARE) in which six aldehyde ketone reductase and alcohol dehydrogenase genes have been knocked out was employed. Then, the carboxylic acid reductase from Neurospora crassa (NcCAR) and phosphopantetheinyl transferase (PPTase) from E. coli were screened for a high conversion rate of cinnamic acid to cinnamaldehyde. …”
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Distinct sets of olfactory receptors highly expressed in different human tissues evaluated by meta-transcriptome analysis: Association of OR10A6 in skin with keratinization by Shinobu Nakanishi, Taiki Tsutsui, Nao Itai, Mitsuhiro Denda

“…An OR10A6 agonist, 3-phenylpropyl propionate (3PPP), transiently increased intracellular Ca2+ concentration and increased cornified envelope (CE) production in cultured keratinocytes. Knock-down of OR10A6 diminished the effect of 3PPP. …”
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Generation of an in vitro model for peripheral neuropathy in Fabry disease using CRISPR-Cas9 in the nociceptive dorsal root ganglion cell line 50B11 by Christine R. Kaneski, John A. Hanover, Ulrike H. Schueler Hoffman

“…Using 50B11 cells, an immortalized dorsal root ganglion neuron with nociceptive characteristics derived from rat, we used CRISPR-Cas9 gene editing of the galactosidase alpha (GLA) gene for AGA to create two stable knock-out clones that have the phenotypic characteristics of Fabry cells. …”
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