Combined analyses of RNA-sequence and Hi-C along with GWAS loci—A novel approach to dissect keloid disorder genetic mechanism by Jia Huang, Xiaobo Zhou, Wenbo Wang, Guangdong Zhou, WenJie Zhang, Zhen Gao, Xiaoli Wu, Wei Liu

“…Among them, the functions of six molecules for cell proliferation, cell cycle and apoptosis were particularly examined and confirmed by overexpressing and knocking-down assays. This study firstly revealed unknown key biomarkers and pathways in keloid lesions using RNA-sequence and previously reported mutation loci, indicating a feasible approach to reveal the genetic contribution to keloid disorder and possibly to other diseases that are failed by GWAS analysis alone. …”
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Receptor tyrosine kinase C-kit promotes a destructive phenotype of FLS in osteoarthritis via intracellular EMT signaling by Xu Cao, Song Wu, Xinxing Wang, Junjie Huang, Wenxiu Zhang, Chi Liang

“…In OA rat model, C-kit largely promoted synovial inflammation and cartilage destruction, whereas knocking-down C-kit significantly restored the health of OA joints. …”
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Strain-dependent modifiers exacerbate familial leukemia caused by GATA1-deficiency by Ikuo Hirano, Kanako Abe, James Douglas Engel, Masayuki Yamamoto, Ritsuko Shimizu

“…Abstract GATA1 plays a critical role in differentiation, proliferation, and apoptosis during erythropoiesis. We developed a Gata1 knock-down allele (Gata1.05) that results in GATA1 expression at 5% of endogenous level. …”
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Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1 by Tiantian Qiu, Donghua Li, Yu Liu, Hui Ren, Xuan Yang, Wenting Luo

“…After sh-IDO1 lentiviral transfection, we discovered that knocking down IDO1 reversed the effects of AS-IV on ULM cell proliferation and autophagy. …”
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DBNLS232 regulates formation of filopodia and promotes cell migration in A549 cells by YIN Xun, YIN Xun, ZHANG Tao, ZHANG Tao

“…Methods DBNL was stably knocked down by shRNA in A549 cells, and then its effects on migration and movement of lung cancer cells were analyzed. …”
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Disruption of O-Linked N-Acetylglucosamine Signaling in Placenta Induces Insulin Sensitivity in Female Offspring by Mackenzie Moore, Nandini Avula, Seokwon Jo, Megan Beetch, Emilyn U. Alejandro

“…The high-fat diet provided a metabolic challenge that revealed a decrease in body weight gain (<i>p</i> = 0.02) and an improved insulin tolerance (<i>p</i> = 0.03) for offspring with a partially reduced placental OGT but not when OGT was fully knocked out. Changes in body weight were not associated with changes in energy homeostasis. …”
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SEPTIN3 Promotes Progression of Triple-Negative Breast Cancer via Activating Wnt Pathway by Wang GZ, Yang LH, Gao C

“…We generated lentivirally transduced TNBC cell lines so such that SEPTIN3 was overexpressed or knocked down. Next, the effect of SEPTIN3 on the biological behavior of TNBC cells was detected using a series of functional assays, including CCK8, colony formation, scratch, and transwell assays. …”
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The Nrf2 Pathway Alleviates Overloading Force-Induced TMJ Degeneration by Downregulating Oxidative Stress Reactions by Xu M, Fang L, Xue Q, Zhang X, He Y

“…MCC degeneration was aggravated when Nrf2 was knocked out, accompanied by increased NOX2 and superoxide dismutase 2 (SOD2) expression. …”
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Cloning of Gossypium hirsutum sucrose non-fermenting 1-related protein kinase 2 gene (GhSnRK2) and its overexpression in transgenic Arabidopsis escalates drought and low temperatur... by Babatunde Bello, Xueyan Zhang, Chuanliang Liu, Zhaoen Yang, Zuoren Yang, Qianhua Wang, Ge Zhao, Fuguang Li

“…GhSnRK2 gene silencing alleviated drought tolerance in cotton plants, indicating that VIGS technique can certainly be used as an effective means to examine gene function by knocking down the expression of distinctly expressed genes. …”
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Maternally Contributed Folate Receptor 1 Is Expressed in Ovarian Follicles and Contributes to Preimplantation Development by Trine Strandgaard, Solveig Foder, Anders Heuck, Erik Ernst, Morten S. Nielsen, Morten S. Nielsen, Karin Lykke-Hartmann, Karin Lykke-Hartmann, Karin Lykke-Hartmann

“…In 2-cell embryos, FOLR1-eGFP fusion protein was detected as cytoplasmic and membrane-associated dense structures, resembling the distribution pattern observed in ovarian follicle development. Knock-down of Folr1 mRNA function was accomplished by microinjection of short interference (si)RNA targeting Folr1, into mouse pronuclear zygotes. …”
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Intervening in hnRNPA2B1-mediated exosomal transfer of tumor-suppressive miR-184-3p for tumor microenvironment regulation and cancer therapy by Xueqing Zhou, Yiling Hong, Yupeng Liu, Li Wang, Xuan Liu, Yi Li, Hong Yuan, Fuqiang Hu

“…Overexpressing miR-184-3p in tumor cells and simultaneously knocking down hnRNPA2B1 to block its secretion through exosomes could effectively inhibit tumor growth and metastasis. …”
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Xanthine Oxidoreductase-Mediated Superoxide Production Is Not Involved in the Age-Related Pathologies in <i>Sod1</i>-Deficient Mice by Shuichi Shibuya, Kenji Watanabe, Yusuke Ozawa, Takahiko Shimizu

“…To investigate the pathological significance of O₂^•− derived from XOR in <i>Sod1</i>^−/− mice, we generated <i>Sod1</i>-null and XO-type- or XDH-type-knock-in (KI) double-mutant mice. Neither XO-type- nor XDH-type KI mutants altered aging-like phenotypes, such as anemia, fatty liver, muscle atrophy, and bone loss, in <i>Sod1</i>^−/− mice. …”
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Acute Ethanol Administration Upregulates Synaptic α4-Subunit of Neuronal Nicotinic Acetylcholine Receptors within the Nucleus Accumbens and Amygdala by Josephine R. Tarren, Henry A. Lester, Arnauld Belmer, Selena E. Bartlett

“…In the present study, we measured the effect of acute binge ethanol on nAChR α4 subunit levels in the prefrontal cortex (PFC), nucleus accumbens (NAc), ventral tegmental area (VTA), and amygdala (Amg) by western blot analysis using a knock-in mouse line, generated with a normally functioning α4 nAChR subunit tagged with yellow fluorescent protein (YFP). …”
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Efficacy of Fowlpox Virus Vector Vaccine Expressing VP2 and Chicken Interleukin-18 in the Protection against Infectious Bursal Disease Virus by Ibrahim Eldaghayes, Lisa Rothwell, Michael Skinner, Abdunaser Dayhum, Pete Kaiser

“…The Fowlpox virus genome itself contains several candidate immunomodulatory genes, including potential IL-18 binding proteins (IL-18bp). We knocked out (Δ) the potential IL-18bp genes in fpIBD1 and inserted (::) the cDNA encoding chIL-18 into fpIBD1 in the non-essential ORF030, generating five new viral constructs –fpIBD1::chIL-18, fpIBD1ΔORF073, fpIBD1ΔORF073::chIL-18, fpIBD1ΔORF214, and fpIBD1ΔORF214::chIL-18. …”
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Insecticide resistance status in Culex quinquefasciatus in Benin by Agnes Yadouléton, Kefilath Badirou, Ramziath Agbanrin, Hanna Jöst, Roseline Attolou, Ramasamy Srinivasan, Gil Padonou, Martin Akogbéto

“…Molecular assays were carried out to detect the presence of knock down resistance (kdr) and acetylcholinesterase (ace. 1) mutations in surviving specimens using PCR techniques. …”
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Vascular Endothelial Integrity Affects the Severity of Enterovirus-Mediated Cardiomyopathy by Jin-Ho Park, Ha-Hyeon Shin, Hyun-Seung Rhyu, So-Hee Kim, Eun-Seok Jeon, Byung-Kwan Lim

“…We have previously shown a hemorrhagic phenotype in germ-line CAR knock-out mouse embryos; we have also found that CAR interacts with ZO-1 and β-catenin. …”
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A Proteomic Study of Hemocyte Proteins from Mud Crab (Scylla paramamosain) Infected with White Spot Syndrome Virus or Vibrio alginolyticus by Fei Zhu, Baozhen Sun, Zhi Wang, Ziyan Wang, Xiongchao Ma

“…During the first 84 h, knockdown of these genes also led to an increase in the morbidity rates in V. alginolyticus -infected crabs, and results of four genes showed a higher mortality rate than that of the control after they were knocked down. This is the first report of the proteome response in crab hemocytes during WSSV or V. alginolyticus infection. …”
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Oleoylethanolamide Protects against Acute Ischemic Stroke by Promoting PPARα-Mediated Microglia/Macrophage M2 Polarization by Ying Li, Yanan Zhang, Qing Wang, Chuang Wu, Guicheng Du, Lichao Yang

“…Transient middle cerebral artery occlusion (tMCAO) was induced for 1 h in wild-type (WT) or PPARα-knock-out (KO) mice. Mouse small glioma cells (BV2) microglia and primary microglia cultures were used to evaluate the direct effect of OEA on microglia. …”
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Genome deletions to overcome the directed loss of gene function in Leishmania by Edubiel A. Alpizar-Sosa, Yasmine Kumordzi, Wenbin Wei, Phillip D. Whitfield, Michael P. Barrett, Michael P. Barrett, Paul W. Denny

“…Strikingly, LCB2 could not be knocked out indicating essentiality. However, simultaneous deletion of LCB2 and the putative ABC3A was possible. …”
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Vascular dysfunction caused by loss of Brn-3b/POU4F2 transcription factor in aortic vascular smooth muscle cells is linked to deregulation of calcium signalling pathways by Vaishaali Yogendran, Laura Mele, Oleksandra Prysyazhna, Vishwanie S. Budhram-Mahadeo

“…Brn-3b protein is expressed in mouse aorta with localisation to VSMCs. Male Brn-3b knock-out (KO) aortas displayed extensive remodelling with increased extracellular matrix (ECM) deposition, elastin fibre disruption and small but consistent narrowing/coarctation in the descending aortas. …”
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