Knockout of Tobacco Homologs of <i>Arabidopsis Multi-Antibiotic Resistance 1</i> Gene Confers a Limited Resistance to Aminoglycoside Antibiotics by Hafizur Rahman, Chika Fukushima, Hidetaka Kaya, Takashi Yaeno, Kappei Kobayashi

“…To explore a possible recessive selective marker for future DNA-free genome editing by direct delivery of a CRISPR/Cas9-single guide RNA (sgRNA) ribonucleoprotein complex, we knocked out homologs of the <i>Arabidopsis</i><i>Multi-Antibiotic Resistance 1</i> (<i>MAR1</i>)<i>/RTS3</i> gene, mutations of which confer aminoglycoside resistance, in tobacco plants by an efficient <i>Agrobacterium-</i>mediated gene transfer. …”
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Effects of Tspan8 Gene Knockout Combined with Anlotinib on Proliferation, Migration, Invasion and Apoptosis of Colon Cancer SW480 Cells by XIE Zhuanhong, XU Yunpeng, MA Huimin, WANG Xiang

“…Methods The plasmid was constructed by CRISPR/Cas9 technique, and Tspan8 gene was knocked out in SW480 cells. The knockout effect was detected by Western blot. …”
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Contribution of Arginine Catabolic Mobile Element and Copper and Mercury Resistance Element in Methicillin-Resistant Staphylococcus aureus: A Vantage Point by Parya Shokrollahi, Alka Hasani, Mohammad Aghazadeh, Mohammad Yousef Memar, Akbar Hasani, Maryam Zaree, Mohammad Ahangarzadeh Rezaee, Javid Sadeghi

“…The presence of ACME increases fitness, thereby allowing bacteria to colonize the skin and mucous membrane while COMER contributes to genetic stability by knocking over the copper-mediated killing in macrophages. …”
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Modeling the interaction between salmon management and consumption by coastal brown bears by William W. Deacy, William B. Leacock, Jonathan B. Armstrong

“…This model creates a potentially useful tool for assessing the knock‐on effects of future changes to salmon management policy.…”
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Local Drug Delivery for Prevention of Hearing Loss by Leonard P. Rybak, Leonard P. Rybak, Asmita Dhukhwa, Debashree Mukherjea, Vickram Ramkumar

“…Administration of siRNAs to knock down AMPK kinase, liver kinase B1 (LKB1) or G9a in the cochlea have been found to ameliorate noise-induced hearing loss. …”
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Incidence of pulmonary tuberculosis under the regular COVID-19 epidemic prevention and control in China by Ziwei Wu, Ziyi Chen, Siyu Long, Aiping Wu, Hongsheng Wang

“…Conclusions Our models quantified the potential knock-on effect on PTB incidence of the intervention strategy used to control the transmission of COVID-19 in China. …”
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Pim1 promotes human prostate cancer cell tumorigenicity and c-MYC transcriptional activity by Roh Meejeon, Kim Jongchan, Abdulkadir Sarki A

“…Interestingly, 10058-F4 treatment also led to a reduction of Pim1 protein but not mRNA. Knocking-down c-MYC using short hairpin RNA reversed the effects of Pim1 on Pim1/MYC target genes.…”
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Intrinsic features of Zika Virus non-structural proteins NS2A and NS4A in the regulation of viral replication. by Yufeng Yu, Chengfeng Gao, Chunxia Wen, Peng Zou, Xian Qi, Carol J Cardona, Zheng Xing

“…Upregulation of these ISGs seemed to be related to the inhibition of ZIKV replication, since the anti-ZIKV function of NS4A was partially attenuated when the cells were treated with Abrocitinib, an inhibitor of the ISGF3 signaling pathway, or were knocked down with STAT2. Aborting the protein expression of NS4A, but not its nucleic acid, eliminated the antiviral activity of NS4A effectively. …”
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Interrogation of human microglial phagocytosis by CRISPR genome editing by Jason Cheng-Yu Chang, Cheng-You Wang, Steven Lin, Steven Lin

“…Co-introduction of DNA repair templates allowed site-specific knock-in (KI) of an epitope tag, a synthetic promoter and a fluorescent reporter gene. …”
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CREB Is Activated by the SCF/KIT Axis in a Partially ERK-Dependent Manner and Orchestrates Survival and the Induction of Immediate Early Genes in Human Skin Mast Cells by Kristin Franke, Gürkan Bal, Zhuoran Li, Torsten Zuberbier, Magda Babina

“…CREB was required for SCF-facilitated survival, as demonstrated with the CREB-selective inhibitor 666-15. Knock-down of CREB by RNA interference duplicated CREB’s anti-apoptotic function. …”
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Inhibition of adult hippocampal neurogenesis induced by postoperative CD8 + T-cell infiltration is associated with cognitive decline later following surgery in adult mice by Xiaowei Li, Hong Wang, Qidi Zhang, Xiaobin Sun, Mengyuan Zhang, Gongming Wang

“…Hippocampal microinjection of IFN-γ neutralizing antibody or adeno-associated virus to knock down IFN-γ receptor 1 (IFNGR1) could also partially attenuate the inhibition of AHN and improve postoperative cognitive function. …”
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Inhibition of HDACs Suppresses Cell Proliferation and Cell Migration of Gastric Cancer by Regulating E2F5 Targeting BCL2 by Arshad Ali, Ayaz Ali, Shaker Khan, Muhammad Ibrahim, Mohammed Ali Alshehri, Anand Thirupathi

“…A <i>p</i> value of < 0.05 was regarded as significant. (3) Results: The present study shows that E2F5 expression is upregulated in GC cancer cell lines compared to normal cell lines, and is positively associated with the level of HDACs and BCL2. HDACi and knocking down of E2F5 as tumor suppressors inhibited cell proliferation, migration invasion, and blocked the cell cycle in gastric cancer cells by suppressing BCL2. …”
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Optimization of Pre-Chamber Geometry and Operating Parameters in a Turbulent Jet Ignition Engine by Viktor Dilber, Momir Sjerić, Rudolf Tomić, Josip Krajnović, Sara Ugrinić, Darko Kozarac

“…The optimization of the pre-chamber volume, the orifice diameter, the injected fuel mass in the pre-chamber and the spark timing was made over 9 different operating points covering the variation in engine speed and load with the objective of minimizing the fuel consumption while avoiding knock. Two optimization methods using 0D/1D simulations were presented: an individual optimization method for each operating point and a simultaneous optimization method over 9 operating points. …”
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TGFβ1-induced Baf60c regulates both smooth muscle cell commitment and quiescence. by Abhishek Sohni, Francesca Mulas, Fulvia Ferrazzi, Aernout Luttun, Riccardo Bellazzi, Danny Huylebroeck, Stephen C Ekker, Catherine M Verfaillie

“…We demonstrated that BAF60c is a necessary co-regulator of TGFβ1 mediated induction of SMC genes. Knock-down of Baf60c decreased SMC gene expression in rMAPCs whereas ectopic expression of Baf60c was sufficient to commit rMAPCs to SMCs in the absence of exogenous cytokines. …”
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Myxoma virus lacking the host range determinant M062 stimulates cGAS-dependent type 1 interferon response and unique transcriptomic changes in human monocytes/macrophages. by Steven J Conrad, Tahseen Raza, Erich A Peterson, Jason Liem, Richard Connor, Bernice Nounamo, Martin Cannon, Jia Liu

“…We found that the replication-defective ΔM062R activated host DNA sensing pathway during infection in a cGAS-dependent fashion and that knocking down SAMD9 expression attenuated proinflammatory responses. …”
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Chrysin, which targets PLAU, protects PC12 cells from OGD/R-stimulated damage through repressing the NF-κB signaling pathway by Ning Li, Ying Liu, Ju-Rong Li, Wen-Xue Zhang

“…Small interfering RNA strategy was employed to knock down the PLAU expression of PC12 cells. The proliferation and apoptosis rates of PC12 cells treated by OGD/R or/and chrysin were detected with Cell Counting Kit 8 (CCK-8) and flow cytometry. …”
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Analysis and verification of the circRNA regulatory network RNO_CIRCpedia_ 4214/RNO-miR-667-5p/Msr1 axis as a potential ceRNA promoting macrophage M2-like polarization in spinal co... by Jian Cao, Chongzhi Pan, Jian Zhang, Qi Chen, Tao Li, Dingwen He, Xigao Cheng

“…RNO_CIRCpedia_4214 was knocked down in vitro, resulting in reduced expression of Msr1, while the expression of RNO-miR-667-5p and Arg1 was increased. …”
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The effect of proteoglycans inhibited by RNA interference on metastatic characters of human salivary adenoid cystic carcinoma by Li Hexiang, Wang Xu, Dong Fusheng, Wang Jie, Shi Hong, Hou Yali

“…</p> <p>In this study, human xylosyltransferase-I (XTLY-I) gene, which catalyzes the rate-limited step of proteoglycans biosynthesis, was knocked down by RNA interference (RNAi) to inhibit the proteoglycans biosynthesis in SACC cell line with high tendency of lung metastasis (SACC-M). …”
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Early chronic suppression of microglial p38α in a model of Alzheimer’s disease does not significantly alter amyloid-associated neuropathology by David J. Braun, Hilaree N. Frazier, Verda A. Davis, Meggie J. Coleman, Colin B. Rogers, Linda J. Van Eldik

“…The present study tests this hypothesis by knocking out microglial p38α and assessing early-stage pathological changes. …”
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The long non‐coding RNA DANCR regulates the inflammatory phenotype of breast cancer cells and promotes breast cancer progression via EZH2‐dependent suppression of SOCS3 transcripti... by Ke‐Jing Zhang, Xiao‐Lang Tan, Lei Guo

“…We compared the expression of DANCR between breast cancer and normal tissues, and between breast cancer cell lines and normal breast epithelial cells using quantitative real‐time PCR (qRT‐PCR) analysis. By knocking down and overexpressing DANCR, we assessed its significance in regulating viability (MTT assay), migration/invasion (Transwell assay), epithelial‐mesenchymal transition (western blot), stemness (mammosphere formation assay and western blot), and production of inflammatory cytokines (qRT‐PCR and ELISA) of breast cancer cells in vitro, as well as xenograft growth in vivo. …”
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