The effect of Nrf2 deletion on the proteomic signature in a human colorectal cancer cell line by Omid Cheraghi, Bahareh Dabirmanesh, Farideh Ghazi, Massoud Amanlou, Mona Atabakhshi-kashi, Yaghoub Fathollahi, Khosro Khajeh

“…Several researches exhibited that Nrf2 could exert multiple functions and expected to be a promising therapeutic target in many cancers. Here, Nrf2 was knocked down in colorectal cancer cell line HT29 and changes that occurred in signaling pathways and survival mechanisms were evaluated. …”
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Dichotomous Effects of Glypican-4 on Cancer Progression and Its Crosstalk with Oncogenes by Victor Chérouvrier Hansson, Fang Cheng, Grigorios Georgolopoulos, Katrin Mani

“…Further, the overexpression of <i>GPC4</i> in <i>GPC4</i>-knocked-down glioblastoma cells restored the proliferation, indicating its mitogenic effect in this cancer type. …”
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MicroRNA-421 Dysregulation is Associated with Tetralogy of Fallot by Douglas C. Bittel, Nataliya Kibiryeva, Jennifer A. Marshall, James E. O'Brien

“…Here, we focus on miR-421, as it is significantly upregulated in RV tissue from infants with TOF; is predicted to interact with multiple members of cardiovascular regulatory pathways; and has been shown to regulate cell proliferation. We knocked down, and over expressed miR-421 in primary cells derived from the RV of infants with TOF, and infants with normally developing hearts, respectively. …”
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Causal factors in primary open angle glaucoma: a phenome-wide Mendelian randomisation study by Thomas H. Julian, Zain Girach, Eleanor Sanderson, Hui Guo, Jonathan Yu, Johnathan Cooper-Knock, Graeme C. Black, Panagiotis I. Sergouniotis

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Waveform-based classification of dentate spikes by Rodrigo M. M. Santiago, Vítor Lopes-dos-Santos, Emily A. Aery Jones, Yadong Huang, David Dupret, Adriano B. L. Tort

“…As an example of application, we used the technique to analyze single-electrode LFPs from apolipoprotein (apo) E3 and apoE4 knock-in mice. We observed that the latter group, which is a model for Alzheimer’s disease, exhibited wider DSs of both types from a young age, with a larger effect size for DS type 2, likely reflecting early pathophysiological alterations in the EC-DG network, such as hyperactivity. …”
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Fuel efficiency enhancement of modified diesel engine operated in dual fuel mode using renewable and sustainable fuels by Sushurth Halewadimath, V. S. Yaliwal, N. R. Banapurmath

“…However, increased hydrogen addition beyond the 12 lpm leads to sever knocking. Abbreviations: NeOME: Neem oil methyl ester; BTE: brake thermal efficiency; CI: compression ignition; ITE: indicated thermal efficiency; PG: producer gas; CA: crank angle; K: Kelvin; BP: brake power; IP: indicated power; H2: hydrogen; HC: unburnt hydrocarbon; CO: carbon dioxide; CO2: carbon dioxide; NOx: nitric oxide; HRR: heat release rate; %: percentage; PPM: parts per million; CMFIS: conventional mechanical fuel injection system.…”
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Developmental delays in cortical auditory temporal processing in a mouse model of Fragile X syndrome by Katilynne Croom, Jeffrey A. Rumschlag, Michael A. Erickson, Devin K. Binder, Khaleel A. Razak

“…Methods In the current study, we quantify auditory temporal processing throughout development in the Fmr1 knock-out (KO) mouse model of Fragile X Syndrome (FXS), a leading genetic cause of intellectual disability and ASD-associated behaviors. …”
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Metabolic engineering of Thermoanaerobacterium aotearoense strain SCUT27 for biofuels production from sucrose and molasses by Kaiqun Dai, Chunyun Qu, Jun Feng, Yang Lan, Hongxin Fu, Jufang Wang

“…To strengthen the alcohols production and substrates metabolism, the redox-sensing transcriptional repressor (rex) in T. aotearoense was further knocked out. Moreover, with the gene arginine repressor (argR) deleted, the ethanologenic mutant P8S10 showed great inhibitors-tolerance and finally accumulated ~ 34 g/L ethanol (a yield of 0.39 g/g sugars) from pretreated cane molasses in 5 L tank by fed-batch fermentation. …”
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Influenza A virus activates cellular Tropomyosin receptor kinase A (TrkA) signaling to promote viral replication and lung inflammation. by Vikram Verma, Mythili Dileepan, Qinfeng Huang, Thu Phan, Wei-Shou Hu, Hinh Ly, Yuying Liang

“…Using a chemical-genetic approach to specifically control TrkA kinase activity through a small molecule compound 1NMPP1 in a TrkA knock-in (TrkA KI) mouse model, we show that 1NMPP1-mediated TrkA inhibition completely protected mice from a lethal IAV infection by significantly reducing viral loads and lung inflammation. …”
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Ehrlichia chaffeensis uses its surface protein EtpE to bind GPI-anchored protein DNase X and trigger entry into mammalian cells. by Dipu Mohan Kumar, Mamoru Yamaguchi, Koshiro Miura, Mingqun Lin, Marek Los, Johannes F Coy, Yasuko Rikihisa

“…Antibody against DNase X or DNase X knock-down by small interfering RNA impaired E. chaffeensis binding, entry, and infection. …”
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Epstein-Barr Virus_Encoded LMP1 upregulates microRNA-21 to promote the resistance of nasopharyngeal carcinoma cells to cisplatin-induced Apoptosis by suppressing PDCD4 and Fas-L. by Guang-Da Yang, Tie-Jun Huang, Li-Xia Peng, Chang-Fu Yang, Ran-Yi Liu, Hong-Bing Huang, Qiao-Qiao Chu, Hong-Jie Yang, Jia-Ling Huang, Zhen-Yu Zhu, Chao-Nan Qian, Bi-Jun Huang

“…These data were confirmed by our results that exogenous LMP1 increased miR-21 in both transiently and stably LMP1-transfected cells, and the knock down of miR-21 substantially reversed the resistance of the NPC cells to cisplatin treatment. …”
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Acetyl Co-A Carboxylase Inhibition Halts Hyperglycemia Induced Upregulation of De Novo Lipogenesis in Podocytes and Proximal Tubular Cells by Pradeep Kayampilly, Nancy Roeser, Thekkelnaycke M Rajendiran, Subramaniam Pennathur, Farsad Afshinnia

“…Exposure to high glucose concentration (25 mM) compared to osmotic controlled low glucose concentration (5.5 mM) significantly increased labeled palmitate after 24 h up to 72 h in podocytes and primary tubular cells. Knocking out of the ACC coding <i>Acaca</i> and <i>Acacb</i> genes by CRISPR/cas9, downregulation of <i>Acaca</i> and <i>Acacb</i> by specific antisense LNA GapmeRs and inhibition of ACC by firsocostat similarly halted/mitigated upregulation of DNL and decreased markers of fibrosis and programmed cell death in podocytes and various tubular cells. …”
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Pathological and neurophysiological outcomes of seeding human-derived tau pathology in the APP-KI NL-G-F and NL-NL mouse models of Alzheimer’s Disease by S. Tok, H. Maurin, C. Delay, D. Crauwels, N. V. Manyakov, W. Van Der Elst, D. Moechars, W. H. I. M. Drinkenburg

“…In this current study, we characterized APP knock-in (APP-KI) animals, that do not overexpress hAPP but still develop amyloid pathology to understand the influence of protein overexpression. …”
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Characterization of essential eggshell proteins from Aedes aegypti mosquitoes by Jun Isoe, Carter J. Simington, Max E. Oscherwitz, Alyssa J. Peterson, Alberto A. Rascón, Brooke B. Massani, Roger L. Miesfeld, Michael A. Riehle

“…Previously, we identified a protein named eggshell organizing factor 1 (EOF1) that when knocked down with RNA interference (RNAi) resulted in non-melanized and fragile eggs that did not contain viable embryos. …”
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Label-Free Investigations on the G Protein Dependent Signaling Pathways of Histamine Receptors by Ulla Seibel-Ehlert, Nicole Plank, Asuka Inoue, Guenther Bernhardt, Andrea Strasser

“…Taking advantage of the integrative nature of the DMR assay, the involvement of endogenous Gα_q/11, Gα_s, Gα_12/13 and Gβγ proteins was explored, pursuing a two-pronged approach, namely that of classical pharmacology (G protein modulators) and that of molecular biology (Gα knock-out HEK cells). We showed that signal transduction of hH_1–4Rs occurred mainly, but not exclusively, via their canonical Gα proteins. …”
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Gene expression analysis after receptor tyrosine kinase activation reveals new potential melanoma proteins by Krause Michael, Samans Birgit, Haydn Johannes M, Teutschbein Janka, Eilers Martin, Schartl Manfred, Meierjohann Svenja

“…The expression of these genes was also monitored in human melanoma cell lines, and the target gene <it>FOSL1 </it>was knocked down by siRNA. Proliferation and migration of siRNA-treated melanoma cell lines were then investigated.…”
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Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells by Yeonghoon Son, Na-Rae Shin, Sung-Ho Kim, Su-Cheol Park, Hae-June Lee

“…Correlations between FGL1 expression and sorafenib resistance were examined by cell viability, colony formation, and flow cytometry assays. FGL1 was knocked-down to confirm its effects on sorafenib resistance. …”
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Orphan GPR26 Counteracts Early Phases of Hyperglycemia-Mediated Monocyte Activation and Is Suppressed in Diabetic Patients by Zahra Abedi Kichi, Lucia Natarelli, Saeed Sadeghian, Mohammad ali Boroumand, Mehrdad Behmanesh, Christian Weber

“…The same mechanisms were activated by HG and exacerbated when GPR26 was knocked down. Hence, our data indicated that GPR26 is initially activated to protect monocytes from HG and is inhibited under chronic hyperglycemic conditions.…”
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Corticotropin-releasing factor-like diuretic hormone acts as a gonad-inhibiting hormone in adult female, Rhodnius prolixus by Areej N. Al-Dailami, Ian Orchard, Angela B. Lange

“…Using RNA interference, transcript expression of Rhopr-CRF/DH-R2 was knocked down, and egg production monitored by examining the major yolk protein, vitellogenin (Vg), the number and quality of eggs laid, and their hatching ratio. …”
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Inhibition of PFKFB3 Expression Stimulates Macrophage-Mediated Lymphangiogenesis Post-Acute Myocardial Infarction by Tianyi Cui, Chao Feng, Hantao Jiang, Ying Jin, Jinping Feng

“…Methods: Intramyocardial injections of adeno-associated viruses (AAV9) to inhibit the expression of VEGFR3 (VEGFR3 shRNA) or promote the expression of VEGFR3 (VEGFR3 ORF) in the heart; Myh6-mCherry B6 D2-tg mice and flow cytometry were used to evaluate the number of myocellular debris in the mediastinal lymph nodes; fluorescence staining and qPCR were used to evaluate fluorescence analysis; seahorse experiment was used to evaluate the level of glycolysis of macrophages; Lyz2𝐶𝑟𝑒, VEGFCfl/fl, and PFKFB3fl/fl mice were used as a model to knock out the expression of VEGF-C and PFKFB3 in macrophages. …”
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