Glutathione S-Transferase pi-1 Knockdown Reduces Pancreatic Ductal Adenocarcinoma Growth by Activating Oxidative Stress Response Pathways by Rahul R. Singh, Jiyan Mohammad, Megan Orr, Katie M. Reindl

“…GSTP1 is overexpressed in breast, colon, lung, and prostate tumors, where it contributes to tumor progression and drug resistance; however, the role of GSTP1 in pancreatic ductal adenocarcinoma (PDAC) is not well understood. Using shRNA, we knocked down GSTP1 expression in three different PDAC cell lines and determined the effect on cell proliferation, cell cycle progression, and reactive oxygen species (ROS) levels. …”
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Redistribution of the SWI/SNF Complex Dictates Coordinated Transcriptional Control over Epithelial–Mesenchymal Transition of Normal Breast Cells through TGF-β Signaling by Sham Jdeed, Máté Lengyel, Iván P. Uray

“…The recruitment of ARID1A and Brg1 was associated with the suppression of TGFBR2, KLF4, and FoxQ1, and the induction of BMP6, while the inverse pattern ensued upon the knock-down of ARID1A. Bex+Carv treatment resulted in fewer cells expressing N-cadherin and dictated a more epithelial phenotype. …”
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Toxoplasma gondii microneme protein MIC3 induces macrophage TNF-α production and Ly6C expression via TLR11/MyD88 pathway. by Jingfan Qiu, Yanci Xie, Chenlu Shao, Tianye Shao, Min Qin, Rong Zhang, Xinjian Liu, Zhipeng Xu, Yong Wang

“…MyD88 inhibition, small interfering RNA against Tlr11 and CRISPR/Cas9-mediated knock-out of Tlr11 all reduced MIC3-induced TNF-α production, NF-κB phosphorylation, iNOS transcription and Ly6C expression. …”
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Evaluating research impact: the development of a ‘RESEARCH for IMPACT’ TOOL by Komla Tsey, Kenny Lawson, Irina Kinchin, Roxanne Bainbridge, Janya McCalman, Felecia Watkin, Yvonne Cadet-James, Allison Rossetto

“…A practical way of approaching research impact is therefore to start with the decisions confronting decision makers whether they are government policymakers, professional practitioners or households and the extent to which the research supports smarter decisions and the knock-on consequences of such smart decisions. Embedded at each step in the impact planning, monitoring and evaluation process is the need for Indigenous leadership and participation, capacity enhancement and collaborative partnerships and participatory learning by doing approaches across partners.Discussion: The tool is designed in the context of Indigenous research but the basic idea that the way to assess research impact is to start upfront by defining the users’ of research and their information needs, the decisions confronting them and the extent to which research informs smarter decisions is equally applicable to research in other settings, both applied and theoretical. …”
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Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p by Nan Wang, Simin Guo, Fengjie Hao, Yifan Zhang, Yongjun Chen, Xiaochun Fei, Junqing Wang

“…The rescue experiment by knock-out miR-126-5p significantly reversed the cell growth suppression and a higher ratio of cell apoptosis induced by SNRPF1 depletion. …”
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A topographical atlas of α-synuclein dosage and cell type-specific expression in adult mouse brain and peripheral organs by Haley M. Geertsma, Zoe A. Fisk, Lillian Sauline, Alice Prigent, Kevin Kurgat, Steve M. Callaghan, aSCENT-PD Consortium, Michael X. Henderson, Maxime W. C. Rousseaux

“…Using a combination of knock-in mice that target αSyn to the nucleus (Snca NLS ) and in situ hybridization of Snca in wild-type mice, we systematically mapped the topography and cell types expressing αSyn in the mouse brain, spinal cord, retina, and gut. …”
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Effect of p53 transcriptional regulatory target gene GGT6 in human colon adenocarcinoma by LIU Zizhao, DU Wenjing, LI Li

“…Nutlin was treated to stabilize p53 or transfected siRNA to knock down p53 expression in HCT116 and HCT8 cells. …”
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T-Type Ca²⁺ Enhancer SAK3 Activates CaMKII and Proteasome Activities in Lewy Body Dementia Mice Model by Jing Xu, Ichiro Kawahata, Hisanao Izumi, Kohji Fukunaga

“…SAK3 enhanced the proteasome activity via CaMKII activation in amyloid precursor protein knock-in mice, promoting the degradation of amyloid-β plaques to improve cognition. …”
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Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism by Zhichao Zhang, Qing Zhang, Shaohua Guan, Hualin Shi

“…The effects of gene deletions on cell size and growth rate are also examined. Knocking out the genes, such as <inline-formula> <math display="inline"> <semantics> <mo>Δ</mo> </semantics> </math> </inline-formula><i>tktA</i>, <inline-formula> <math display="inline"> <semantics> <mo>Δ</mo> </semantics> </math> </inline-formula><i>tktB</i>, <inline-formula> <math display="inline"> <semantics> <mo>Δ</mo> </semantics> </math> </inline-formula><i>yqaB</i>, <inline-formula> <math display="inline"> <semantics> <mo>Δ</mo> </semantics> </math> </inline-formula><i>pgm</i>, and <inline-formula> <math display="inline"> <semantics> <mo>Δ</mo> </semantics> </math> </inline-formula><i>cysQ</i>, affects growth rate largely while affecting cell length slightly. …”
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In Vivo Regulation of the μ Opioid Receptor: Role of the Endogenous Opioid Agents by Veronica Gonzalez-Nunez, Ada Jimenez González, Katherine Barreto-Valer, Raquel E. Rodríguez

“…Treatment with opioid peptides (Met-enkephalin (Met-ENK), Met-enkephalin-Gly-Tyr (MEGY) and β-endorphin (β-END)) modulated dre-oprm1 expression during development. Knocking down the dre-oprm1 gene significantly modified the mRNA expression of the penk and pomc genes, thus indicating that oprm1 is involved in shaping penk and pomc expression. …”
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METTL3/MALAT1/ELAVL1 Axis Promotes Tumor Growth in Ovarian Cancer by Xiong J, Lian W, Zhao R, Gao K

“…The proliferation rate of OC cells was reduced significantly after knocking down the expression of METTL3 or MALAT1. Subsequently, MALAT1 as oncogene was found to interact with METTL3 and was upregulated in OC tissues and cells. …”
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Electric field stimulation unmasks a subtle role for T-type calcium channels in regulating lymphatic contraction by Michael J. Davis, Jorge A. Castorena-Gonzalez, Scott D. Zawieja

“…Abstract We previously identified two isoforms of T-type, voltage-gated calcium (Cav3) channels (Cav3.1, Cav3.2) that are functionally expressed in murine lymphatic muscle cells; however, contractile tests of lymphatic vessels from single and double Ca v 3 knock-out (DKO) mice, exhibited nearly identical parameters of spontaneous twitch contractions as wild-type (WT) vessels, suggesting that Cav3 channels play no significant role. …”
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Deubiquitinase USP1 enhances CCAAT/enhancer-binding protein beta (C/EBPβ) stability and accelerates adipogenesis and lipid accumulation by Myung Sup Kim, Jung-Hwan Baek, JinAh Lee, Aneesh Sivaraman, Kyeong Lee, Kyung-Hee Chun

“…We found that USP1 directly interacts with C/EBPβ. Knock-down of USP1 decreased C/EBPβ protein stability and increased its ubiquitination. …”
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aThe characteristics of glucose metabolism in the sulfonylurea receptor 1 knockout rat model by Xiaojun Zhou, Chunmei Xu, Zhiwei Zou, Xue Shen, Tianyue Xie, Rui Zhang, Lin Liao, Jianjun Dong

“…Results Insulin secretion was significantly inhibited by knocking out the SUR1 gene. SUR1 −/− rats showed lower body weights compared to wild-type rats, and even SUR1 −/− males weighed less than wild-type females. …”
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Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, autophagy, and YB-1 independent manner. by Tomozumi Imamichi, Qian Chen, Bharatwaj Sowrirajan, Jun Yang, Sylvain Laverdure, Mayra Marquez, Anthony R Mele, Catherine Watkins, Joseph W Adelsberger, Jeanette Higgins, Hongyan Sui

“…Cross-reference analysis with the reported 2,214 HIV regulatory host genes identified nine genes as potential interests: Ankyrin repeat domain 22, Guanylate binding protein (GBP)-1, -2, -4, -5, Stabilin 1, Serpin family G member 1 (SERPING1), Interferon alpha inducible protein 6, and Interferon-induced protein with tetratricopeptide repeats 3. A knock-down study using si-RNA failed to determine a key factor associated with the anti-HIV activity due to the induction of robust amounts of off-target effects. …”
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Free fatty acid receptor 4 (FFA4) activation attenuates obese asthma by suppressing adiposity and resolving metaflammation by So-Eun Son, Jung-Min Koh, Dong-Soon Im

“…We investigated whether FFA4 activation ameliorates obese asthma using an FFA4 agonist, compound A (CpdA), in combination with FFA4 wild-type (WT) and knock-out (KO) mice. Administration of an FFA4 agonist, compound A (CpdA, 30 mg/kg), suppressed HFD-induced weight gain, adiposity, and airway hypersensitivity (AHR), and increased immune cell infiltration in an FFA4-dependent manner. …”
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LncRNA SNHG12 promotes cell proliferation and inhibits apoptosis of granulosa cells in polycystic ovarian syndrome by sponging miR-129 and miR-125b by Feilan Xuan, Ruiying Jin, Weimei Zhou, Yongju Ye, Yuefang Ren, Jiali Lu, Aixue Chen

“…A loss-of-functions test was implemented by co-transfecting miR-129 and miR-125b inhibitors into lncRNA SNHG12-knocking down cells to analyze the effects on cell viability and apoptosis. …”
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Lysine-Specific Demethylase 4D Is Critical for the Regulation of the Cell Cycle and Antioxidant Capacity in Goat Fibroblast Cells by Xiaowei Chen, Yingnan Yang, Yu Cai, Hua Yang, Feng Wang, Mingtian Deng

“…Moreover, both the mRNA and protein expression levels of superoxide dismutase 2 (SOD2), one of the major antioxidant enzymes, was decreased, while the reactive oxygen species (ROS) level was significantly increased in Kdm4d knocked-down cells. In addition, the expression of γH2A histone family member X (γH2AX) increased significantly, indicating the presence of DNA double-strand breaks after the knockdown of the Kdm4d enzyme. …”
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Attenuated levels of hippocampal connexin 43 and its phosphorylation correlate with antidepressant- and anxiolytic-like activities in mice by Gaël eQuesseveur, Benjamin ePortal, Jean-Arnaud eBasile, Pascal eEzan, Alexia eMathou, Hélène eHalley, Corinne eLeloup, Xavier eFioramonti, Nicole eDéglon, Christian eGiaume, Claire eRampon, Bruno Pierre Guiard, Bruno Pierre Guiard

“…The first aim of this study was to evaluate the impact of the constitutive knock-down of Cx43 on a set of behaviors known to be affected in depression. …”
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Fish female-biased gene cyp19a1a leads to female antiviral response attenuation between sexes by autophagic degradation of MITA. by Long-Feng Lu, Jing-Yu Jiang, Wen-Xuan Du, Xue-Li Wang, Zhuo-Cong Li, Xiao-Yu Zhou, Can Zhang, Cheng-Yan Mou, Dan-Dan Chen, Zhi Li, Li Zhou, Jian-Fang Gui, Xi-Yin Li, Shun Li

“…Furthermore, the autophagy factor ATG14 (autophagy-related 14) was found interacted with Cyp19a1a to either promote or attenuate Cyp19a1a-mediated MITA degradation by either being overexpressed or knocked down, respectively. At the cellular level, both the normal and MITA-enhanced cellular antiviral responses were diminished by Cyp19a1a. …”
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