Atheroprone fluid shear stress-regulated ALK1-Endoglin-SMAD signaling originates from early endosomes by Paul-Lennard Mendez, Leon Obendorf, Jerome Jatzlau, Wiktor Burdzinski, Maria Reichenbach, Vanasa Nageswaran, Arash Haghikia, Verena Stangl, Christian Hiepen, Petra Knaus

“…Results We report here that knock-down of Endoglin leads to exacerbated SMAD1/5 phosphorylation and atheroprone gene expression profile in HUVECs sheared for 24 h. …”
Get full text

Increased Lipogenesis Is Important for Hexavalent Chromium-Transformed Lung Cells and Xenograft Tumor Growth by James T. F. Wise, Kazuya Kondo

“…We observed that FASN inhibition results in a decreased protein expression, decreased cell growth, and the inhibition of colony growth in soft agar. Next, using shRNA to knock down the FASN protein in Cr(VI)-transformed BEAS-2B cells, we saw a decrease in FASN protein expression and a loss of the xenograft tumor development of Cr(VI)-transformed BEAS-2B cells. …”
Get full text

DOK3 promotes proliferation and inhibits apoptosis of prostate cancer via the NF-κB signaling pathway by Kun Jin, Shi Qiu, Bo Chen, Zilong Zhang, Chichen Zhang, Xianghong Zhou, Lu Yang, Jianzhong Ai, Qiang Wei, Yuanyuan Ji

“…A subcutaneous xenograft mouse model was performed to examine phenotypes after knocking down DOK3 in vivo. Rescue experiments with DOK3 knockdown and NF-κB pathway activation were designed to verify regulating effects. …”
Get full text

RPLP1 Is Up-Regulated in Human Adenomyosis and Endometrial Adenocarcinoma Epithelial Cells and Is Essential for Cell Survival and Migration In Vitro by Riley Peterson, Paige Minchella, Wei Cui, Amanda Graham, Warren B. Nothnick

“…Endometrial adenocarcinoma cell lines, Ishikawa, HEC1A, HEC1B and AN3 were evaluated for RPLP1 protein and transcript expression, while in vitro function was evaluated by knocking down RPLP1 expression and assessing cell survival and migration. …”
Get full text

AKT1 Transcriptomic Landscape in Breast Cancer Cells by Bijesh George, Bin Gui, Rajeswari Raguraman, Aswathy Mary Paul, Harikrishna Nakshatri, Madhavan Radhakrishna Pillai, Rakesh Kumar

“…Here, we delineate the impact of selective AKT1 knock down using gene-specific siRNAs or inhibiting the AKT activity with a pan-AKT inhibitor VIII on the nature of transcriptomic changes in breast cancer cells using the genome-wide RNA-sequencing analysis. …”
Get full text

Knockdown of DEC2 expression inhibits the proliferation of mesangial cells through suppressed glycolysis and p38 MAPK/Erk pathway in lupus nephritis by Huimeng Qi, Li Xu, Qiang Liu

“…Methods The 32-week-old female Fcgr2b−/− mice and their serum-treated MCs were used as in vivo and in vitro LN model. MCs knocked down of DEC2 and overexpressed with DEC2 were also established. …”
Get full text

OASL Expression in Pancreatic Cancer and Its Effect on Proliferation and Migration of Pancreatic Cancer Cells by ZHANG Rendan, ZHAO Chunyan, YAO Jiaxin, HU Xianhua, MU Bo

“…The TCGA database was used to analyze the correlation of OASL expression with the clinicopathological parameters of pancreatic cancer. shRNA was used to knock down the expression of OASL gene in pancreatic cancer panc-1 cells. …”
Get full text

#222 : Exosomes Derived of Menstrual Blood Stem Cells Suppress IUA Endometrial Fibrosis Through P65-UBR4-YAP Pathway by Jichun Tan, Jiarui Qi, Xudong Zhang

“…Method: IUA rats and endometrial stromal cells (EndoSCs) of IUA patients were treated with knock-down UBR4 exosomes (EXOUBR4−) and normal exosomes (EXO). …”
Get full text

Potent Inhibitory Effect of BJ-3105, a 6-Alkoxypyridin-3-ol Derivative, on Murine Colitis Is Mediated by Activating AMPK and Inhibiting NOX by Pallavi Gurung, Sadan Dahal, Prakash Chaudhary, Diwakar Guragain, Ujjwala Karmacharya, Jung-Ae Kim, Byeong-Seon Jeong

“…Moreover, BJ-3105 suppressed the more severe form of colitis and CAT formation in mice with AMPK knocked-out in macrophages (<i>AMPKα</i>^fl/fl-<i>Lyz2</i>-Cre mice) with much greater efficacy than tofacitinib. …”
Get full text

Long non-coding RNA CCL2 promoted gastric cancer function via miR-128/ PARP2 signal pathway by Liang Ma, Yunshan Jiang, Ning Wu

“…More importantly, miR-128 could reverse the effects of lncRNA CCL2 knocked down. PARP2-si obviously suppressed in gastric cancer proliferation, migration, and invasion. …”
Get full text

Cytokine enrichment in deep cerebellar nuclei is contributed by multiple glial populations and linked to reduced amyloid plaque pathology by Jessica R. Gaunt, Norliyana Zainolabidin, Alaric K. K. Yip, Jia Min Tan, Aloysius Y. T. Low, Albert I. Chen, Toh Hean Ch’ng

“…Abstract Alzheimer’s disease (AD) pathology and amyloid-beta (Aβ) plaque deposition progress slowly in the cerebellum compared to other brain regions, while the entorhinal cortex (EC) is one of the most vulnerable regions. Using a knock-in AD mouse model (App KI), we show that within the cerebellum, the deep cerebellar nuclei (DCN) has particularly low accumulation of Aβ plaques. …”
Get full text

m7G regulator-mediated molecular subtypes and tumor microenvironment in kidney renal clear cell carcinoma by Mei Chen, Zhenyu Nie, Yuanhui Gao, Hui Cao, Linlin Zheng, Na Guo, Yanling Peng, Shufang Zhang

“…Patients with high m7G scores were more sensitive to rapamycin, gefitinib, sunitinib, and vinblastine than other patients. Knocking down WDR4 can inhibit the proliferation, migration, and invasion of 786-0 and Caki-1 cells and increase sensitivity to sorafenib and sunitinib.Conclusion: We proposed a novel molecular subtype related to m7G modification and revealed the immune cell infiltration characteristics of different subtypes. …”
Get full text

Deciphering the Role of Emx1 in Neurogenesis: A Neuroproteomics Approach by Firas H Kobeissy, katharina Hansen, Melanie Neumann, Shuping Fu, Kunlin Jin, Jialing Liu

“…To decipher the role of Emx1 gene in neural regeneration and the timing of its involvement, we determine the frequency of neural stem cells (NSCs) in embryonic and adult forebrains of Emx1 wild type (WT) and knock out (KO) mice in the neurosphere assay. Emx1 gene deletion reduced the frequency and self-renewal capacity of neural stem cells (NSCs) of the embryonic brain but did not affect neuronal or glial differentiation. …”
Get full text

LncRNA H19-rich extracellular vesicles derived from gastric cancer stem cells facilitate tumorigenicity and metastasis via mediating intratumor communication network by Hongying Zhao, Rongke Jiang, Chunmei Zhang, Zhijing Feng, Xue Wang

“…Methods CSCs and non-stem cancer cells (NSCCs) were sorted from GC cells, and EVs were isolated from CSCs. H19 was knocked down in CSCs, and CSCs-EVs or CSCs-EVs containing shRNA-H19 (CSCs-EVs-sh-H19) were co-cultured with NSCCs, followed by evaluation of the malignant behaviors and stemness of NSCCs. …”
Get full text

Acid sphingomyelinase mediates ferroptosis induced by high glucose via autophagic degradation of GPX4 in type 2 diabetic osteoporosis by Yun-xia Du, Yan-tao Zhao, Yong-xin Sun, Ai-hua Xu

“…To further investigate the specific mechanism, we examined these indicators by knocking down ASM expression, hydroxychloroquine (HCQ) treatment, or N-acetylcysteine (NAC) treatment. …”
Get full text

Astrocytic Connexin43 Channels as Candidate Targets in Epilepsy Treatment by Laura Walrave, Mathieu Vinken, Luc Leybaert, Ilse Smolders

“…Human Cx43 mutations are linked to seizures as well, as 30% of patients with oculodentodigital dysplasia (ODDD), a rare genetic condition caused by mutations in the GJA1 gene coding for Cx43 protein, exhibit neurological symptoms including seizures. Cx30/Cx43 double knock-out mice show increased susceptibility to evoked epileptiform events in brain slices due to impaired GJ-mediated redistribution of K⁺ and glutamate and display a higher frequency of spontaneous generalized chronic seizures in an epilepsy model. …”
Get full text

Sushi domain-containing protein 4 binds to epithelial growth factor receptor and initiates autophagy in an EGFR phosphorylation independent manner by Konstantinos S. Papadakos, Alexander Ekström, Piotr Slipek, Eleni Skourti, Steven Reid, Kristian Pietras, Anna M. Blom

“…Moreover, we established a cell line based on BT-20 in which the gene for EGFR was knocked out with the CRISPR-Cas9 method. Results We discovered that the Epithelial Growth Factor Receptor (EGFR) interacts with SUSD4. …”
Get full text

Critical window in autism : a study on Shank3 by Mei, Yuan (Yuan Karen)

Get full text

Improving efficacy of therapeutics by enhancing delivery using chemical engineering by Tam, Hok Hei

Get full text

Shank Proteins Differentially Regulate Synaptic Transmission by Shi, Rebecca D., Redman, Patrick, Ghose, Dipanwita, Hwang, Hongik, Liu, Yan, Ren, Xiaobai, Ding, Lei J., Liu, Mingna, Jones, Kendrick, Xu, Weifeng

“…Only when both Shank1 and Shank2 were knocked down, were both the number and the unitary response of active synapses reduced. …”
Get full text