The RNA‐binding protein RBMS3 inhibits the progression of colon cancer by regulating the stability of LIMS1 mRNA by Yafei Li, Shuoshuo Wang, Guoli Li, Chunyang Gao, Zihan Cui, Mingqi Cong, Jie Hu, Minghui Zhang, Xiaoming Jin, Haiying Sun, Dan Kong

“…The inhibitory function of LIMS1 and RBMS3 is closely intertwined in colon cancer, with knocking down LIMS1 being able to rescue the inhibitory effect of RBMS3 overexpression on the functionality of colon cancer cell Conclusions The discernments delineate RBMS3 as a novel suppressor of cancer via LIMS1, thereby bestowing fresh therapeutic possibilities and illuminating the intricacies of colon cancer.…”
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Circadian rhythm of PERIOD2::LUCIFERASE expression in the trigeminal ganglion of mice by Yukie Shirakawa, Sachi N. Ohno, Kanae A. Yamagata, Eriko Kuramoto, Yoshiaki Oda, Takahiro J. Nakamura, Wataru Nakamura, Mitsutaka Sugimura

“…To measure gene expression as bioluminescence, PERIOD2::LUCIFERASE knock-in (PER2::LUC) mice were used. Unilateral trigeminal ganglion and brain sections including the suprachiasmatic nucleus were incubated ex vivo. …”
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Carbon Metabolism of a Soilborne Mn(II)-Oxidizing <i>Escherichia coli</i> Isolate Implicated as a Pronounced Modulator of Bacterial Mn Oxidation by Tong Gu, Zhenghu Tong, Xue Zhang, Zhiyong Wang, Zhen Zhang, Tzann-Shun Hwang, Lin Li

“…A total of 16 carbon metabolism-related genes with significant variant expression levels under Mn supplementation conditions were knocked out in the MB266 genome accordingly, but only little effect on the MnODA of each mutant strain was accounted for. …”
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Fusobacterium nucleatum-derived small extracellular vesicles facilitate tumor growth and metastasis via TLR4 in breast cancer by Guiqiu Li, Yan Sun, Yu Huang, Jie Lian, Shaoyuan Wu, Dixian Luo, Hui Gong

“…Fn-EVs administration significantly enhanced the cell viability, proliferation, migration, and invasion of BC cells, while knocking down TLR4 in BC cells could block these effects. …”
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PSME4 Degrades Acetylated YAP1 in the Nucleus of Mesenchymal Stem Cells by Yong Sook Kim, Mira Kim, Dong Im Cho, Soo Yeon Lim, Ju Hee Jun, Mi Ra Kim, Bo Gyeong Kang, Gwang Hyeon Eom, Gaeun Kang, Somy Yoon, Youngkeun Ahn

“…Injection of immortalized human MSCs after knocking down PSME4 failed to improve either cardiac function or the fibrotic scar area. …”
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Hnf4α Is Involved in LC-PUFA Biosynthesis by Up-Regulating Gene Transcription of Elongase in Marine Teleost Siganus canaliculatus by Yuanyou Li, Xiaowei Zeng, Yewei Dong, Cuiying Chen, Cuihong You, Guoxia Tang, Junliang Chen, Shuqi Wang

“…Moreover, overexpression of hnf4&alpha; caused a remarkable increase both in elovl5 promoter activity and mRNA contents, while knock-down of hnf4&alpha; in S. canaliculatus hepatocyte line (SCHL) resulted in a significant decrease of elovl5 gene expression. …”
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Conditional deletion of MAD2B in forebrain neurons enhances hippocampus-dependent learning and memory in mice by Li Cheng, Yanfang Su, Kaining Zhi, Yaru Xie, Yaru Xie, Chun Zhang, Xianfang Meng

“…In this study, we crossed MAD2Bflox/flox and calcium/calmodulin-dependent protein kinase II alpha (Camk2a)-Cre mice to conditionally knock out MAD2B in the forebrain pyramidal neurons by the Cre/loxP recombinase system. …”
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NSUN2 promotes osteosarcoma progression by enhancing the stability of FABP5 mRNA via m5C methylation by Min Yang, Renxiong Wei, Sheng Zhang, Sang Hu, Xiaoxiao Liang, Zhiqiang Yang, Chong Zhang, Yufeng Zhang, Lin Cai, Yuanlong Xie

“…We observed that NSUN2 stabilized FABP5 mRNA by inducing m5C modification and further promoted fatty acid metabolism in OS cells. Moreover, both knocking down the expression of FABP5 and adding fatty acid oxidation inhibitor could counterbalance the promoting effect of NSUN2 on the progression of OS. …”
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Codon Optimization Enables the Geneticin Resistance Gene to Be Applied Efficiently to the Genetic Manipulation of the Plant Pathogenic Fungus <i>Botrytis cinerea</i> by Maoyao Tang, Yangyizhou Wang, Kexin Wang, Yuanhang Zhou, Enshuang Zhao, Hao Zhang, Mingzhe Zhang, Hang Yu, Xi Zhao, Guihua Li

“…Using the gene replacement method, we successfully knocked out the second gene <i>BOT2</i>, with <i>BcNPTII</i> as the selection marker, from the mutant Δ<i>oahA</i>, in which <i>OAHA</i> was first replaced by the hygromycin resistance gene <i>HPH</i> in a field strain. …”
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Human SCARB2 Acts as a Cellular Associator for Helping Coxsackieviruses A10 Infection by Shu-Ling Yu, Nai-Hsiang Chung, Yu-Ching Lin, Yi-An Liao, Ying-Chin Chen, Yen-Hung Chow

“…Our research has shown that CVA10 can infect and replicate in mouse cells expressing human SCARB2 (3T3-SCARB2) but not in the parental NIH3T3 cells, which do not express hSCARB2 for CVA10 entry. Knocking down endogenous hSCARB2 and KREMEN1 with specific siRNAs inhibited CVA10 infection in human cells. …”
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Downregulation of DNMT3A Attenuates the Warburg Effect, Proliferation, and Invasion via Promoting the Inhibition of miR-603 on HK2 in Ovarian Cancer by Jiaojiao Lu PhD, Shuai Zhen PhD, Xiaoqian Tuo BD, Shixue Chang BD, Xiling Yang MD, Yuanyuan zhou PhD, Wei Chen PhD, Le Zhao PhD, Xu Li PhD

“…The higher the RNA level of DNMT3A, the poorer prognosis of patients. DNMT3A knocking down impeded the Warburg effect, cell proliferation, migration, and invasion of ovarian cancer cells. …”
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COL2-dependent photoperiodic floral induction in Nicotiana sylvestris seems to be lost in the N. sylvestris × N. tomentosiformis hybrid N. tabacum by Florentin J. Schmidt, Lena Grundmann, Michael Lahme, Marvin Seidemann, Axel Schwarze, Sophie Lichtenauer, Richard M. Twyman, Dirk Prüfer, Dirk Prüfer, Gundula A. Noll, Gundula A. Noll

“…NtCOL2a/b overexpression or knock-out using the CRISPR/Cas9 system did not support a substantial role for the CO homologs in the control of floral transition in N. tabacum. …”
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Identification of microtubule-associated biomarkers in diffuse large B-cell lymphoma and prognosis prediction by Wenqi Wu, Su Liu, Linyan Tian, Cheng Li, Yanan Jiang, Jinhuan Wang, Yangyang Lv, Jing Guo, Donghui Xing, Yixin Zhai, Huimeng Sun, Yuhang Li, Luying Zhang, Xiang He, Kaiping Luo, Hongjie Zhan, Zhigang Zhao, Zhigang Zhao

“…The K-M curve presented that the high-risk patients had a significantly inferior overall survival (OS) time compared to low-risk patients in training and validation datasets. Furthermore, knocking-out TMEM63A, a key gene belonging to the MAGs model, inhibited cell proliferation noticeably.Conclusion: The novel MAGs prognostic model has a well predictive capability, which may as a supplement for the current assessments. …”
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RNA interference of Aspergillus flavus in response to Aspergillus flavus partitivirus 1 infection by Yinhui Jiang, Yinhui Jiang, Xiang Liu, Xiang Liu, Xun Tian, Xun Tian, Jianhong Zhou, Jianhong Zhou, Qinrong Wang, Qinrong Wang, Bi Wang, Bi Wang, Wenfeng Yu, Wenfeng Yu, Yanping Jiang, Tom Hsiang, Xiaolan Qi, Xiaolan Qi

“…In our study, reverse genetics and virus-derived small RNA sequencing were used to show the antiviral responses of RNAi components in Aspergillus flavus infected with Aspergillus flavus partitivirus 1 (AfPV1). qRT-PCR revealed that AfPV1 infection induced the expression of the RNAi components in A. flavus compared with noninfected A. flavus. Knock mutants of each RNAi component were generated, but the mutants did not exhibit any obvious phenotypic changes compared with the A. flavus parental strain. …”
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Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and inflammasome activation by Ji‐Min Dai, Xue‐Qin Zhang, Jia‐Jia Zhang, Wei‐Jie Yang, Xiang‐Min Yang, Huijie Bian, Zhi‐Nan Chen

“…To address the issue, we generated the human il‐6r knock‐in mice and a defined epitope murine anti‐human membrane‐bound IL‐6R (mIL‐6R) mAb named h‐mIL‐6R mAb. …”
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A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli by Ming Zhao, Yuting Li, Fengqing Wang, Yuhong Ren, Dongzhi Wei

“…First, a higher ITA-yielding strain, Δ4-Prmd-SAS-3 (ITA titer: 3.20 g/L), derived from Δ1-SAS-3, was constructed by replacing the promoter PJ23100, for the expression of ITA synthesis genes, with Prmd and knocking out the three bypass genes poxB, pflB, and ldhA. …”
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MiR-1286 inhibits lung cancer growth through aerobic glycolysis by targeting PKM2 by Hui Li, Xuexia Lin, Chaopeng Li, Jungai Li, Xunxun Xu, Dongyue Meng, Shushen Zheng

“…Material and methods The mRNA levels of miR-1286 in NSCLC tissues and mouse tumor tissues were detected by q-PCR. MiR-1286 was knocked down and overexpressed separately in A549 cells. …”
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RETRACTED ARTICLE: TRIM11 overexpression promotes proliferation, migration and invasion of lung cancer cells by Xiaolin Wang, Weiping Shi, Hongcan Shi, Shichun Lu, Kang Wang, Chao Sun, Jiansheng He, Weiguo Jin, Xiaoxia Lv, Hui Zou, Yusheng Shu

“…The activity of ERK and PI3K/AKT was also suppressed in TRIM11 knocked down cells. Further experiments in lung cells with lower expression of TRIM11 (NCI-H460 and NCI-H1975 cells) with AKT inhibitor suggested that TRIM11 may promote cell motility and invasiveness through AKT pathway. …”
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PTH-Induced Bone Regeneration and Vascular Modulation Are Both Dependent on Endothelial Signaling by Doron Cohn-Schwartz, Yeshai Schary, Eran Yalon, Zoe Krut, Xiaoyu Da, Edward M. Schwarz, Dan Gazit, Gadi Pelled, Zulma Gazit

“…In addition to endothelial-specific PTHR1 knock-out mice, we used mice in which PTHR1 was engineered to be constitutively active in collagen-1α+ osteoblasts, to assess the effect of PTH signaling activation exclusively in osteoprogenitors. …”
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LncRNA TDRG1-Mediated Overexpression of VEGF Aggravated Retinal Microvascular Endothelial Cell Dysfunction in Diabetic Retinopathy by Qiaoyun Gong, Wenpei Dong, Ying Fan, Feng’e Chen, Xiaolan Bian, Xun Xu, Tianwei Qian, Tianwei Qian, Ping Yu

“…Transfection of small-interfering RNA was conducted to knock down target gene expression. HREC functions were evaluated by cell viability, fluorescein isothiocyanate (FITC)-dextran extravasation, migration, and tube formation assays under different conditions.ResultsLncRNA TDRG1 and VEGF were found to be co-expressed and significantly upregulated in fibrovascular membranes (FVMs) from DR patients compared to those from EM patients. …”
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