Functional and developmental changes in the inner hair cell ribbon synapses caused by Myosin VI knockout and deafness-inducing point mutation by Ning Yin, Jingjing Zhao, Panpan Zhang, Baofu Yu, Renjie Chai, Geng-Lin Li

“…In this study, we used both knock-out (Myo6 −/−) and point mutation (Myo6 C442Y ) mice as animal models, performed whole-cell patch-clamp recording and capacitance measurement in the inner hair cells (IHCs) in the cochlea, and sought to reveal potential functional and developmental changes in their ribbon synapses. …”
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MicroRNA-30b Is Both Necessary and Sufficient for Interleukin-21 Receptor-Mediated Angiogenesis in Experimental Peripheral Arterial Disease by Tao Wang, Liang Yang, Mingjie Yuan, Charles R. Farber, Rosanne Spolski, Warren J. Leonard, Vijay C. Ganta, Brian H. Annex

“…In EC cultured under hypoxia serum starvation, knock-down of miR-30b reduced, while overexpression of miR-30b increased IL-21-mediated EC survival and angiogenesis. …”
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IL-10 Promotes CXCL13 Expression in Macrophages Following Foot-and-Mouth Disease Virus Infection by Zijing Guo, Fei Chen, Shuaiyang Zhao, Zhixiong Zhang, Huijun Zhang, Ling Bai, Zhidong Zhang, Yanmin Li

“…Interestingly, CXCL13 concentration was decreased in sera from interleukin-10 knock out (IL-10^-/-) mice or mice blocked IL-10/IL-10R signaling in vivo after FMDV infection. …”
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PD-L1 Inhibits T Cell-Induced Cytokines and Hyaluronan Expression via the CD40-CD40L Pathway in Orbital Fibroblasts From Patients With Thyroid Associated Ophthalmopathy by Zhibin Liu, Yao Liu, Mingming Liu, Qingjia Gong, Anjie Shi, Xiuhong Li, Xiuhong Li, Xu Bai, Xiaoyue Guan, Bing Hao, Feila Liu, Xing Zhou, Xing Zhou, Xing Zhou, Hongfeng Yuan, Hongfeng Yuan

“…Additionally, exogenous PD-L1 treatment also inhibits the expression of CD40 and the phosphorylation levels of MAPK and NF-κB pathways in orbital fibroblasts of the OF-T cell co-culture system. Knocking down CD40 with CD40 siRNA or down-regulating the phosphorylation levels of MAPK and NF-κB pathways with SB203580, PD98059, SP600125, and PDTC can both reduce the expression of these cytokines and hyaluronan. …”
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Lactate receptor GPR81 drives breast cancer growth and invasiveness through regulation of ECM properties and Notch ligand DLL4 by Kathrine Lundø, Oksana Dmytriyeva, Louise Spøhr, Eliana Goncalves-Alves, Jiayi Yao, Laia P. Blasco, Mette Trauelsen, Muthulakshmi Ponniah, Marc Severin, Albin Sandelin, Marie Kveiborg, Thue W. Schwartz, Stine F. Pedersen

“…Methods GPR81 was stably knocked down (KD) in MCF-7 human breast cancer cells which were subjected to RNA-seq analysis, 3D growth, in situ- and immunofluorescence analyses, and cell viability- and motility assays, combined with KD of key GPR81-regulated genes. …”
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Loss of GIPR in LEPR cells impairs glucose control by GIP and GIP:GLP-1 co-agonism without affecting body weight and food intake in mice by Seun Akindehin, Arkadiusz Liskiewicz, Daniela Liskiewicz, Miriam Bernecker, Cristina Garcia-Caceres, Daniel J. Drucker, Brian Finan, Gerald Grandl, Robert Gutgesell, Susanna M. Hofmann, Ahmed Khalil, Xue Liu, Perla Cota, Mostafa Bakhti, Oliver Czarnecki, Aimée Bastidas-Ponce, Heiko Lickert, Lingru Kang, Gandhari Maity, Aaron Novikoff, Sebastian Parlee, Ekta Pathak, Sonja C. Schriever, Michael Sterr, Siegfried Ussar, Qian Zhang, Richard DiMarchi, Matthias H. Tschöp, Paul T. Pfluger, Jonathan D. Douros, Timo D. Müller

“…Long-acting single- and dual-agonists at GIPR and GLP-1R were further used to assess drug effects on energy and glucose metabolism in DIO wildtype (WT) and Lepr-Gipr knock-out (KO) mice. Results: Gipr and Lepr show strong co-expression in the pancreas, but not in the hypothalamus and hindbrain. …”
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Insecticide susceptibility status in two medically important mosquito vectors, Anopheles gambiae, and Culex quinquefasciatus to three insecticides commonly used in Niger State, Nig... by Ibrahim Kura Shehu, Hamdan Bn Ahmad, Israel Kayode Olayemi, Danjuma Solomon, Abu Hassan Ahmad, Hasber Salim

“…Post-exposure mortality after 24hr and knockdown values for KDT50 were calculated. Knock-down at 1-hour insecticide exposure ranged (84–96 %) permethrin, (94–100 %) propoxur and (100 %) malathion for An. gambiae and (86–97 %) permethrin, (92–100 %) propoxur and (96–100 %) malathion for Cx. quinquefasciatus. …”
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Development of a Novel Anti-EpCAM Monoclonal Antibody for Various Applications by Guanjie Li, Hiroyuki Suzuki, Teizo Asano, Tomohiro Tanaka, Hiroyoshi Suzuki, Mika K. Kaneko, Yukinari Kato

“…In contrast, recEpMab-37 did not react with EpCAM-knocked out Caco-2 cells. The <i>K</i>_D of recEpMab-37 for CHO/EpCAM and Caco-2 was 2.0 × 10⁻⁸ M and 3.2 × 10⁻⁸ M, respectively. …”
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Rice Chalky Grain 5 regulates natural variation for grain quality under heat stress by Anil Kumar Nalini Chandran, Jaspreet Sandhu, Larissa Irvin, Puneet Paul, Balpreet K. Dhatt, Waseem Hussain, Tian Gao, Paul Staswick, Hongfeng Yu, Gota Morota, Harkamal Walia

“…These findings are supported by increased chalkiness of OsCG5 knock-out (KO) mutants relative to wildtype (WT) under heat stress. …”
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A Tumor Microenvironment-Driven Network Regulated by STAT3 and p65 Negatively Controls the Enrichment of Cancer Stem Cells in Human HR+/HER2− Breast Cancer by Hagar Ben-Yaakov, Tsipi Meshel, Metsada Pasmanik-Chor, Cindy Körner, Adit Ben-Baruch

“…Upon TME Stimulation, stattic (STAT3 inhibitor) usage demonstrated that Y705-STAT3 activation negatively controlled CSC enrichment and epithelial-to-mesenchymal transition (EMT) traits, while inducing CXCL8 (IL-8) and PD-L1 expression. However, STAT3 knock-down (siSTAT3) had no effect on these functions; in terms of CSC enrichment, p65 had down-regulatory roles that compensated for the loss of an entire STAT3 protein. …”
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TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB by Ji Xing, Wu Chen, Kang Chen, Shaoming Zhu, Fangyou Lin, Yucheng Qi, Yunlong Zhang, Shangting Han, Ting Rao, Yuan Ruan, Sheng Zhao, Weimin Yu, Fan Cheng

“…Subsequently, in vitro, we knocked down TFAP2C in BCa cells and found that TFAP2C knockdown further increased cell cycle arrest and apoptosis caused by cisplatin. …”
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TFEB Overexpression, Not mTOR Inhibition, Ameliorates RagC^S75Y Cardiomyopathy by Maengjo Kim, Linghui Lu, Alexey V. Dvornikov, Xiao Ma, Yonghe Ding, Ping Zhu, Timothy M. Olson, Xueying Lin, Xiaolei Xu

“…We generated a zebrafish Rragc^S56Y (corresponding to human RagC^S75Y) knock-in (KI) line via TALEN technology. The KI fish manifested cardiomyopathy-like phenotypes and poor survival. …”
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Low levels of nitric oxide promotes heme maturation into several hemeproteins and is also therapeutic by Arnab Ghosh, Mamta P. Sumi, Blair Tupta, Toshihiro Okamoto, Kulwant Aulak, Masato Tsutsui, Hiroaki Shimokawa, Serpil C. Erzurum, Dennis J. Stuehr

“…These effects of low NO were significantly diminished in the tissues of double (n/eNOS−/−) and triple (n/i/eNOS−/−) NOS knock out mice where lung sGC was found be heme-free and the myoglobin or hemoglobin from the heart/lungs were found be low in heme, suggesting that loss of endogenous NO globally impacts the whole animal and that this impact of low NO is both essential and physiologically relevant for hemeprotein maturation. …”
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Serine-arginine splicing factor 2 promotes oesophageal cancer progression by regulating alternative splicing of interferon regulatory factor 3 by Ziqing Wei, Yuyao Wang, Wenyuan Ma, Wenqing Xing, Peng Lu, Zhijie Shang, Feng Li, Huiyu Li, Yuxuan Wang

“…Results We found upregulation of SRSF2 is correlated with the development of EC; Knock-down of SRSF2 inhibits EC cell proliferation, migration, and invasion; SRSF2 regulates the splicing pattern of IRF3 in EC cells; SRSF2 interacts with exon 2 of IRF3 to regulate its exclusion; SRSF2 inhibits the transcription of IFN1 in EC cells. …”
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Induction of Cyclooxygenase-2 by Overexpression of the Human NADPH Oxidase 5 (NOX5) Gene in Aortic Endothelial Cells by Javier Marqués, Adriana Cortés, Álvaro Pejenaute, Eduardo Ansorena, Gloria Abizanda, Felipe Prósper, Juan José Martínez-Irujo, Carlos de Miguel, Guillermo Zalba

“…For that purpose, two experimental systems were employed: an adenoviral NOX5-&#946; overexpression model in immortalized human aortic endothelial cells (TeloHAEC) and a chronic infarction in vivo model developed from a conditional endothelial NOX5 knock-in mouse. NOX5 increased cyclooxygenase-2 isoform (COX-2) expression and prostaglandin E₂ (PGE₂) production through nuclear factor kappa-light-chain-enhancer of activated B cells (NF-&#954;B) in TeloHAEC. …”
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A novel potent class I HDAC inhibitor reverses the STAT4/p66Shc apoptotic defect in B cells from chronic lymphocytic leukemia patients by Sara Rossi, Vanessa Tatangelo, Maria Dichiara, Stefania Butini, Sandra Gemma, Simone Brogi, Silvia Pasquini, Martina Cappello, Fabrizio Vincenzi, Katia Varani, Ludovica Lopresti, Margherita Malchiodi, Chiara Carrara, Alessandro Gozzetti, Monica Bocchia, Giuseppe Marotta, Laura Patrussi, Gabriele Carullo, Cosima T. Baldari, Giuseppe Campiani

“…The role of HDAC1, but not HDAC3, in the epigenetic upregulation of STAT4/p66Shc was demonstrated for the first time in CLL cells and was validated in siRNA-induced HDAC1/HDAC3 knock-down EBV-B cells. To sum up, HDAC1 inhibition is necessary to reactivate STAT4/p66Shc expression in patients with CLL. 6c is one of the most potent HDAC1is known to date and represents a novel pharmacological tool for reversing the impairment of the STAT4/p66Shc apoptotic machinery.…”
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Compatible solutes determine the heat resistance of conidia by Sjoerd J. Seekles, Tom van den Brule, Maarten Punt, Jan Dijksterhuis, Mark Arentshorst, Maryam Ijadpanahsaravi, Winfried Roseboom, Gwendolin Meuken, Véronique Ongenae, Jordy Zwerus, Robin A. Ohm, Gertjan Kramer, Han A. B. Wösten, Johannes H. de Winde, Arthur F. J. Ram

“…Transcriptomic and proteomic analyses revealed two putative heat shock proteins to be upregulated under these conditions. However, conidia of knock-out strains lacking these putative heat shock proteins did not show a reduced heat resistance. …”
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PSTPIP2 ameliorates aristolochic acid nephropathy by suppressing interleukin-19-mediated neutrophil extracellular trap formation by Changlin Du, Chuanting Xu, Pengcheng Jia, Na Cai, Zhenming Zhang, Wenna Meng, Lu Chen, Zhongnan Zhou, Qi Wang, Rui Feng, Jun Li, Xiaoming Meng, Cheng Huang, Taotao Ma

“…We found that renal tubular epithelial cell (RTEC) apoptosis, neutrophil infiltration, inflammatory factor, and NET production were increased in a mouse model of AAN, while PSTPIP2 expression was low. Conditional knock-in of Pstpip2 in mouse kidneys inhibited cell apoptosis, reduced neutrophil infiltration, suppressed the production of inflammatory factors and NETs, and ameliorated renal dysfunction. …”
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Neuronal loss of TRPM8 leads to obesity and glucose intolerance in male mice by D. Liskiewicz, Q. Zhang, C.S. Barthem, M. Jastroch, A. Liskiewicz, N. Khajavi, G. Grandl, C. Coupland, M. Kleinert, C. Garcia-Caceres, A. Novikoff, G. Maity, U. Boehm, M.H. Tschöp, T.D. Müller

“…Methods: Nestin Cre- and Advillin Cre-Trpm8 knock-out (KO) mice were metabolically phenotyped under chronic exposure to either chow or high-fat diet (HFD), followed by assessment of energy and glucose metabolism. …”
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Mitogen and Stress-Activated Kinases 1 and 2 Mediate Endothelial Dysfunction by Naveed Akbar, Calum Forteath, Muhammad S. Hussain, Kathleen Reyskens, Jill J. F. Belch, Chim C. Lang, Ify R. Mordi, U Bhalraam, J. Simon C. Arthur, Faisel Khan

“…Using translational vascular function testing in myocardial infarction patients, a situation where inflammation is prevalent, and knock-out (KO) mouse models we demonstrate a role for mitogen-activated-protein-kinases (MAPKs) in endothelial dysfunction. …”
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