Myeloid checkpoint blockade improves killing of T-acute lymphoblastic leukemia cells by an IgA2 variant of daratumumab by Niklas Baumann, Christian Arndt, Judith Petersen, Marta Lustig, Thies Rösner, Katja Klausz, Christian Kellner, Miriam Bultmann, Lorenz Bastian, Fotini Vogiatzi, Jeanette H. W. Leusen, Renate Burger, Denis M. Schewe, Matthias Peipp, Thomas Valerius

“…In order to investigate the impact of this myeloid checkpoint on T-ALL cell killing, CD47 and glutaminyl-peptide cyclotransferase like (QPCTL) knock-out T-ALL cells were employed. QPTCL is an enzymatic posttranslational modifier of CD47 activity, which can be targeted by small molecule inhibitors. …”
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HX009, a novel BsAb dual targeting PD1 x CD47, demonstrates potent anti-lymphoma activity in preclinical models by Hang Ke, Faming Zhang, Jingjing Wang, Lingxin Xiong, Xiaoyu An, Xiaolong Tu, Cen Chen, Yueying Wang, Binchen Mao, Sheng Guo, Cunxiang Ju, Xiangfei He, Ruilin Sun, Lei Zhang, Owen A. O’Connor, Qi-Xiang Li

“…In the humanized mouse syngeneic A20 B-lymphoma (huCD47-A20) HuGEMM model, which has quadruple knocked-in hPD1xhPD-L1xhCD47xhSIRPα genes and an intact autologous immune-system, a contribution of effect is demonstrated for each targeted biologic (HX008 targeting PD1 and SIRPα-Fc targeting CD47), which is clearly augmented by the dual targeting with HX009. …”
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TREM2 is associated with tumor immunity and implies poor prognosis in glioma by Mingchen Yu, Yuanhao Chang, You Zhai, Bo Pang, Peng Wang, Guanzhang Li, Guanzhang Li, Tao Jiang, Tao Jiang, Tao Jiang, Tao Jiang, Tao Jiang, Fan Zeng, Fan Zeng

“…Immunofluorescent staining indicated that knocking down the expression of TREM2 would result in a decrease in M2 polarization. …”
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Elucidating the role of EPPK1 in lung adenocarcinoma development by Ken Arimura, Michael Kammer, S. M. Jamshedur Rahman, Chen Sheau-Chiann, Shilin Zhao, Chen Heidi, Rosana Eisenberg, Yong Zou, Sanja Antic, Bradley Richmond, Etsuko Tagaya, Eric Grogan, Pierre Massion, Fabien Maldonado

“…Further, we used CRISPR-Cas9 to knock out (KO) EPPK1 in LUAD cell lines and observed how the cancer cells were altered functionally and genetically. …”
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Novel roles of DC-SIGNR in colon cancer cell adhesion, migration, invasion, and liver metastasis by Heya Na, Xiaoli Liu, Xiaomeng Li, Xinsheng Zhang, Yu Wang, Zhaohui Wang, Menglang Yuan, Yu Zhang, Shuangyi Ren, Yunfei Zuo

“…Results In our research, we investigated whether DC-SIGNR promotes colon cancer cell adhesion, migration, and invasion. Knocking down mouse DC-SIGNR decreased the liver metastatic potency of colon cancer cells and increased survival time. …”
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A Genome-Wide Functional Screen Identifies Enhancer and Protective Genes for Amyloid Beta-Peptide Toxicity by Pol Picón-Pagès, Mònica Bosch-Morató, Laia Subirana, Francisca Rubio-Moscardó, Biuse Guivernau, Hugo Fanlo-Ucar, Melisa Ece Zeylan, Simge Senyuz, Víctor Herrera-Fernández, Rubén Vicente, José M. Fernández-Fernández, Jordi García-Ojalvo, Attila Gursoy, Ozlem Keskin, Baldomero Oliva, Francesc Posas, Eulàlia de Nadal, Francisco J. Muñoz

“…To identify novel molecular players in Aβ toxicity, we carried out a genome-wide screen in <i>Saccharomyces cerevisiae</i>, using a library of 5154 gene knock-out strains expressing Aβ_1–42. We identified 81 mammalian orthologue genes that enhance Aβ_1–42 toxicity, while 157 were protective. …”
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Induced Resistance Combined with RNA Interference Attenuates the Counteradaptation of the Western Flower Thrips by Tao Zhang, Li Liu, Yulian Jia, Junrui Zhi, Wenbo Yue, Dingyin Li, Guang Zeng

“…Co-silencing with RNAi simultaneously knocked down <i>FoGSTd1</i> and <i>FoGSTs1</i> transcripts and GST activity, and the area damaged by second-instar larvae feeding on JA- and MeJA-induced leaves decreased by 62.22% and 55.24%, respectively. …”
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Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin... by Lujia Feng, Haichun Li, Yong Du, Ting Zhang, Yingting Zhu, Zhidong Li, Ling Zhao, Xing Wang, Gongpei Wang, Linbin Zhou, Zhaorong Jiang, Zheng Liu, Zhancong Ou, Yuwen Wen, Yehong Zhuo

“…In the present study, we specifically knocked down MERTK and CCT5 through siRNA transfection and examined the expression of CCT5 and F-actin in human primary RPE (HsRPE) cells. …”
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Translationally controlled tumor protein restores impaired memory and altered synaptic protein expression in animal models of dementia by Eun Jung Na, Yejin Jeon, Hyunju Kim, Hye-Sun Kim, Kyunglim Lee, Hwa-Jung Kim

“…Specifically, memory functions and expression levels of hippocampal synaptic proteins in 7- to 12-month-old SCO-treated wild-type (WT-SCO) mice were compared to those of TCTP-overexpressing (TG) and TCTP knocked-down (KD) mice similarly treated with SCO. …”
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Nrf2 expression and function, but not MT expression, is indispensable for sulforaphane-mediated protection against intermittent hypoxia-induced cardiomyopathy in mice by Shanshan Zhou, Jiqun Wang, Xia Yin, Ying Xin, Zhiguo Zhang, Taixing Cui, Jun Cai, Yang Zheng, Quan Liu, Lu Cai

“…Here, we investigated whether SFN can up-regulate cardiac Nrf2 expression and function, as well as MT expression, to prevent IH-induced cardiomyopathy, and if so, whether Nrf2 and MT are indispensable for this preventive effect. Nrf2-knock-out (Nrf2-KO) or MT-KO mice and their wild-type (WT) equivalents were exposed to IH for 4 weeks with or without SFN treatment. …”
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PTEN and AKT/GSK-3β/CRMP-2 signaling pathway are involved in neuronal apoptosis and axonal injury in early brain injury after SAH in rats by Hong Chen, Chao Zhou, Jianfeng Zheng, Zhaosi Zhang, Yongbing Deng, Chongjie Cheng, Zongduo Guo, Gang Huo, Cheng Yin, Xiaochuan Sun

“…The mechanism was analyzed by Western blot to detect p-PTEN/PTEN, p-AKT/AKT, p-GSK-3β/GSK-3β, p-CRMP-2/CRMP-2, axonal injury marker β-APP and pro- and anti-apoptosis proteins, including Bax and Bcl-2, expression. We found 1. After knocking down PTEN, neuronal apoptosis and axonal injury were alleviated, and nerve function and blood–brain barrier were protected; accordingly, after overexpression of PTEN, neuronal apoptosis and axon damage were aggravated, and nerve function damage and blood–brain barrier damage were increased. 2. …”
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Identification of INFG/STAT1/NOTCH3 as γ-Mangostin's potential targets for overcoming doxorubicin resistance and reducing cancer-associated fibroblasts in triple-negative breast ca... by Bashir Lawal, Alexander TH Wu, Chien-Hsin Chen, George T.A, Szu-Yuan Wu

“…We then demonstrated that gMG treatment reduced the tumorigenic properties similarly observed in STAT1-knocked down conditions. Finally, we utilized a DOX-resistant TNBC tumoroid-bearing mouse model to demonstrate that gMG treatment significantly delayed tumor growth, reduced CAF generation, and improved DOX sensitivity. …”
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Sex-Specific ADHD-like Behaviour, Altered Metabolic Functions, and Altered EEG Activity in Sialyltransferase ST3GAL5-Deficient Mice by Tatyana Strekalova, Ekaterina Veniaminova, Evgeniy Svirin, Ekaterina Kopeikina, Tatyana Veremeyko, Amanda W. Y. Yung, Andrey Proshin, Shawn Zheng Kai Tan, Sharafuddin Khairuddin, Lee Wei Lim, Klaus-Peter Lesch, Susanne Walitza, Daniel C. Anthony, Eugene D. Ponomarev

“…Disruption of ganglioside production by this enzyme may also have a role in the development of neuropsychiatric disorders. ST3Gal5 knock-out (<i>St3gal5^−/−</i>) mice lack a-, b-, and c-series gangliosides, but exhibit no overt neuropathology, possibly owing to the production of compensatory 0-series glycosphingolipids. …”
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A missense mutation in the Hspa8 gene encoding heat shock cognate protein 70 causes neuroaxonal dystrophy in rats by Miyuu Tanaka, Miyuu Tanaka, Ryoko Fujikawa, Takahiro Sekiguchi, Jason Hernandez, Oleta T. Johnson, Daisuke Tanaka, Kenta Kumafuji, Tadao Serikawa, Hieu Hoang Trung, Kosuke Hattori, Tomoji Mashimo, Mitsuru Kuwamura, Jason E. Gestwicki, Takashi Kuramoto, Takashi Kuramoto

“…Furthermore, we developed the Hspa8 knock-in (KI) rats with the V95E mutation using the CRISPR-Cas system. …”
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Effects of Ethanol on Expression of Coding and Noncoding RNAs in Murine Neuroblastoma Neuro2a Cells by Mi Ran Choi, Sinyoung Cho, Dai-Jin Kim, Jung-Seok Choi, Yeung-Bae Jin, Miran Kim, Hye Jin Chang, Seong Ho Jeon, Young Duk Yang, Sang-Rae Lee

“…We validated the differential expression of some mRNAs and lncRNAs by RT-qPCR and analyzed the expression of <i>Cebpd</i> and <i>Rnu3a</i> through knock-down of <i>Cebpd</i>. Cell proliferation was significantly reduced in cells exposed to 100 mM ethanol for 72 h, with 1773 transcripts up- or downregulated by greater than three-fold in ethanol-treated cells compared to controls. …”
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Caveolin-1 identified as a key mediator of acute lung injury using bioinformatics and functional research by Lihua Qu, Yi Li, Chao Chen, Tong Yin, Qian Fang, Yijin Zhao, Wenting Lv, Ziqi Liu, Yangye Chen, Li Shen

“…Subsequently, the expression of CAV-1 was knocked down using AAV-shCAV-1 or CAV-1-siRNA to study its effect on the pathogenesis of ALI in vivo and in vitro. …”
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GATA6 regulates anti-angiogenic properties in human cardiac fibroblasts via modulating LYPD1 expression by Shinako Masuda, Katsuhisa Matsuura, Tatsuya Shimizu

“…Endothelial cell network formation was attenuated when co-cultured with cardiac fibroblasts, but it was significantly restored when co-cultured with cardiac fibroblasts wherein the expression of GATA6 was knocked down with siRNA. Conclusion: GATA6 regulate the anti-angiogenic properties of cardiac fibroblasts by modulating LYPD1 expression.…”
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TXNIP aggravates cardiac fibrosis and dysfunction after myocardial infarction in mice by enhancing the TGFB1/Smad3 pathway and promoting NLR... by Zhang Yan, Wang Jin, Wang Xuejiao, Li Aiyun, Lei Zhandong, Li Dongxue, Xing Dehai, Zhang Yichao, Su Wanzhen, Jiao Xiangying

“…In the present study, we investigate the biological role of TXNIP in post-MI cardiac fibrosis and the underlying mechanism using mouse MI models of the wild-type (WT), Txnip-knockout (Txnip-KO) type and Txnip-knock-in (Txnip-KI) type. After MI, the animals present with significantly upregulated TXNIP levels, and their fibrotic areas are remarkably expanded with noticeably impaired cardiac function. …”
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Lead exposure disturbs ATP7B-mediated copper export from brain barrier cells by inhibiting XIAP-regulated COMMD1 protein degradation by Yang Liu, Zai-Hua Zhao, Tao Wang, Jin-Yu Yao, Wen-Qing Wei, Li-Hong Su, Shuang-Shuang Tan, Zi-Xuan Liu, Han Song, Jing-Yuan Chen, Wei Zheng, Wen-Jing Luo, Gang Zheng

“…Pb-induced Cu accumulation and ATP7B expression were reduced when COMMD1 was knocked down by transient small interfering RNA (siRNA) transfection. …”
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Non-genetic adaptive resistance to KRASG12C inhibition: EMT is not the only culprit by Wenjuan Ning, Wenjuan Ning, Thomas M. Marti, Thomas M. Marti, Patrick Dorn, Patrick Dorn, Ren-Wang Peng, Ren-Wang Peng

“…A recent breakthrough in cancer treatment is the development of KRASG12C inhibitors, which herald a new era of therapy by knocking out a unique substitution of an oncogenic driver. …”
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