Serum Exosomal Long Noncoding RNA Growth Arrest-Specific 5 Predicts 3-Month Mortality in Acute-on-Chronic Hepatitis B Liver Failure by Sun CX, Han LY, Wang K, Gao S

“…Exosomes-mediated lncRNA GAS5 transfer promoted hepatocytes injury. The knocked down of lncRNA GAS5 weakened H2O2-induced hepatocytes injury.Conclusion: We revealed that serum exosomal lncRNA GAS5 might promote hepatocytes injury and showed high predictive value for 3-month mortality in ACHBLF.Keywords: acute-on-chronic hepatitis B liver failure, exosomes, long noncoding RNA, growth arrest-specific 5, prognosis…”
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Single-cell sequencing reveals CD133+CD44−-originating evolution and novel stemness related variants in human colorectal cancer by Xiaoyan Zhang, Ling Yang, Wanjun Lei, Qiang Hou, Ming Huang, Rongjing Zhou, Tariq Enver, Shixiu Wu

“…Then, we set up a mutation panel from scWES data and TES was used to validate mutation distribution and clonal evolution in additional 96 samples (20 patients) those were also sorted into the same three groups of CRCICs and CRCCs. The knock-down experiments were used to analyze stemness-related mutant genes. …”
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Sodium salicylate reduced mRNA abundance of hypoxia-associated genes in MAC-T cells by C.M. Ylioja, T.H. Swartz, L.K. Mamedova, B.J. Bradford

“…Small interfering RNA knocked down HIF1A, but no effect was found on GLUT1, VEGFA, or HNRNPD. …”
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TNF-Mediated Inhibition of Classical Swine Fever Virus Replication Is IRF1-, NF-κB- and JAK/STAT Signaling-Dependent by Matthias Liniger, Markus Gerber, Sandra Renzullo, Obdulio García-Nicolás, Nicolas Ruggli

“…In order to investigate the signaling involved in the antiviral activity of TNF, we employed small-molecule inhibitors to interfere specifically with JAK/STAT and NF-κB signaling pathways in near-to-primary endothelial PEDSV.15 cells. In addition, we knocked out selected factors of the interferon (IFN) induction and signaling pathways using CRISPR/Cas9. …”
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NR2F2 Regulates Cell Proliferation and Immunomodulation in Whartons’ Jelly Stem Cells by Li Ma, Min Huang, Xiaohua Liao, Xiyu Cai, Qiang Wu

“…However, it remains unknown how NR2F2 regulates stem cell identity in WJ-MSCs. (2) Methods: We used RNAi technology to knock down NR2F2 in WJ-MSCs, and studied the regulatory role of NR2F2 in WJ-MSCs by MTT, flow cytometry, RNA-seq, and other methods. …”
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Two NIS1-like proteins from apple canker pathogen (Valsa mali) play distinct roles in plant recognition and pathogen virulence by Jiajun Nie, Wenjing Zhou, Yonghui Lin, Zhaoyang Liu, Zhiyuan Yin, Lili Huang

“…Both VmNIS1 and VmNIS2 were shown to be highly induced at late stage of V. mali infection. By individually knocking out of these two genes in V. mali, however, only VmNIS2 was shown to be required for pathogen virulence as well as tolerance to oxidative stress. …”
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PGC-1α and MEF2 Regulate the Transcription of the Carnitine Transporter OCTN2 Gene in C2C12 Cells and in Mouse Skeletal Muscle by Katerina Novakova, Michael Török, Miljenko Panajatovic, Jamal Bouitbir, François H. T. Duong, Christoph Handschin, Stephan Krähenbühl

“…Due to the observation that the muscle OCTN2 mRNA level is maintained in PPAR-α knock-out mice and that PGC-1α overexpression in C2C12 myoblasts increases OCTN2 mRNA expression, we suspected additional regulatory pathways for SLC22A5 gene transcription. …”
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Two strategies to improve the supply of PKS extender units for ansamitocin P-3 biosynthesis by CRISPR–Cas9 by Siyu Guo, Xueyuan Sun, Ruihua Li, Tianyao Zhang, Fengxian Hu, Feng Liu, Qiang Hua

“…To enhance a “glycolate” extender unit, two combined bidirectional promoters (BDPs) ermEp-kasOp and j23119p-kasOp were knocked into asm12-asm13 spacer in the center region of gene cluster, respectively, by pCRISPR–Cas9apre. …”
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ST6GAL1 is associated with poor response to chemoradiation in rectal cancer by Mary Smithson, Sameer Al Diffalha, Regina K. Irwin, Gregory Williams, M. Chandler McLeod, Vivek Somasundaram, Susan L. Bellis, Karin M. Hardiman

“…Methods: Five organoid models were created from primary human rectal cancers and ST6GAL1 was knocked down via lentivirus transduction in one model. …”
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An immune infiltration-related prognostic model of kidney renal clear cell carcinoma with two valuable markers: CAPN12 and MSC by Guang Xia, Song Wu, Xiaoyu Cui

“…For validation, the 786-O cell line was used to carry out the dual-gRNA approach to knock down CAPN12 and MSC. This was followed by qRT-PCR to verify the knockdown of CAPN12 and MSC.ResultsFor ccRCC, seven predictive genes were discovered: PVT1, MSC, ALDH6A1, TRIB3, QRFPR, CYS1, and CAPN12. …”
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Multi-Omics Analysis and Verification of the Oncogenic Value of CCT8 in Pan-Cancers by Gong L, Zhong M, Gong K, Wang Z, Zhong Y, Jin Y, Chen H, Tai P, Chen X, Chen A, Cao K

“…CCT8 played a vital role in the cell cycle and RNA transport of cancers, and it significantly inhibited the proliferation and migration of lung adenocarcinoma cells when it was knocked down.Conclusion: CCT8 plays an indispensable role in promoting the proliferation and migration of many cancers. …”
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m6A-modification regulated circ-CCT3 acts as the sponge of miR-378a-3p to promote hepatocellular carcinoma progression by Hua Liu, Yifan Jiang, Jiahua Lu, Chuanhui Peng, Zhenan Ling, Yunhao Chen, Diyu Chen, Rongliang Tong, Shusen Zheng, Jian Wu

“…Circ-CCT3 expression served as an independent risk factor for overall survival in patients with HCC. Knocking-down of circ-CCT3 inhibited the proliferation, invasion and migration of HCC cells, and angiogenesis of HUVEC. …”
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Hepatocyte Nuclear Factor 4α (HNF4α) Plays a Controlling Role in Expression of the Retinoic Acid Receptor β (<i>RARβ</i>) Gene in Hepatocytes by Reza Zolfaghari, Jessica A. Bonzo, Frank J. Gonzalez, A. Catharine Ross

“…The expression of the <i>RARβ</i> gene in the liver of HNF4α knock-out mice was higher versus wildtype controls, whereas oppositely, <i>RARβ</i> promoter activity was 50% reduced by the overexpression of HNF4α in HepG2 cells, and treatment with retinoic acid (RA), a major metabolite of vitamin A, increased <i>RARβ</i> promoter activity 15-fold. …”
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Construction and experimental validation of an acetylation-related gene signature to evaluate the recurrence and immunotherapeutic response in early-stage lung adenocarcinoma by Haiqiang Wang, Xiyan Lu, Jiakuan Chen

“…Accordingly, a novel gene signature containing RBBP7 and YEATS2 was developed to evaluate the recurrence-free survival of early-stage LUAD, which was then validated in five independent cohorts (pooled hazard ratio = 1.88, 95% confidence interval = 1.49–2.37) and 23 local clinical samples (P < 0.01). The knock-down of YEATS2 obviously suppressed proliferation of H1975 and HCC-827 cells. …”
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Reduced neuronal size and mTOR pathway activity in the Mecp2 A140V Rett syndrome mouse model [version 1; referees: 2 approved] by Sampathkumar Rangasamy, Shannon Olfers, Brittany Gerald, Alex Hilbert, Sean Svejda, Vinodh Narayanan

“…We have created a mouse model (Mecp2 A140V “knock-in” mutant) expressing the recurrent human MECP2 A140V mutation linked to an X-linked mental retardation/Rett syndrome phenotype. …”
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NR1B2 suppress kidney renal clear cell carcinoma (KIRC) progression by regulation of LATS 1/2-YAP signaling by Lei Yin, Wenjia Li, Guangchun Wang, Heng Shi, Keyi Wang, Huan Yang, Bo Peng

“…KIRC cell line were stably overexpressed NR1B2 or with an NR1B2 knocked down using lentivirus system. The cells were analyzed by migration and invasion assay, then injected into nude mice to assess tumor growth and metastasis. …”
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IL-1R1 signaling in TBI: assessing chronic impacts and neuroinflammatory dynamics in a mouse model of mild closed-head injury by Jonathan C. Vincent, Colleen N. Garnett, James B. Watson, Emma K. Higgins, Teresa Macheda, Lydia Sanders, Kelly N. Roberts, Ryan K. Shahidehpour, Eric M. Blalock, Ning Quan, Adam D. Bachstetter

“…Using a closed-head injury (CHI) TBI model, we investigated IL-1's role in the neuroinflammatory cascade with a new global knock-out (gKO) mouse model of the IL-1 receptor (IL-1R1), which efficiently eliminates all IL-1 signaling. …”
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The Phase-Dependent Regulation of Lux-Type Genes on the Spoilage Characteristics of <i>Hafnia alvei</i> by Jingran Bi, Qiaoli Yao, Gongliang Zhang, Hongman Hou

“…The auto-inducer synthase gene <i>luxI</i> and a regulatory gene <i>luxR</i> of the quorum sensing systems in <i>H. alvei</i> H4 were knocked out to construct the mutant phenotypes. On this basis, the research found that the <i>luxI</i> and <i>luxR</i> genes had a strong positive influence on not only flagella-dependent swimming ability and biofilm formation but also the production of putrescine and cadaverine. …”
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Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors by Jing Chen, Jie Wu, Xiang Chen, Qian Tao, Nian Liu, Cong Peng

“…Mechanistically, Mef upregulated the expression of LPCAT3, a key gene involved in lipid peroxidation, by activating IFN-γ-induced STAT1-IRF1 signaling, and knocking down LPCAT3 impaired the induction of ferroptosis by Mef+IFN-γ. …”
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A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines by Josep Escrivá-Fernández, Cristina Cueto-Ureña, Amalia Solana-Orts, Elisa Lledó, Begoña Ballester-Lurbe, Enric Poch

“…Additionally, the impact of Rnd3 expression absence was analyzed by a transcriptomic analysis consisting of 3’ UTR RNA sequencing. The Rnd3 knock-down cells showed a different transcriptomic profile in comparison to control cells. …”
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