MMP9-Associated Tumor Stem Cells, CCL1-Silenced Dendritic Cells, and Cytokine-Induced Killer Cells Have a Remarkable Therapeutic Efficacy for Acute Myeloid Leukemia by Activating T... by Min Dong, Guozhen Zhang, Jie Meng, Biou Liu, Duanfeng Jiang, Feng Liu

“…Furthermore, activated T cells in DC-CIK cells knocking down MMP9 and CCL1 also prevented proliferation and accelerated apoptosis of AML cells. …”
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Silencing of secreted phosphoprotein 1 attenuates sciatic nerve injury‐induced neuropathic pain: Regulating extracellular signal‐regulated kinase and neuroinflammatory signaling pa... by Haiyu Xie, Feng Lu, Xiaoling Li, Enfu Wang, Jiao Mo, Weidong Liang

“…Methods We established a chronic constriction injury (CCI) rat model, knocked down SPP1 via an intrathecal injection, and/or activated the extracellular signal‐regulated kinase (ERK) pathway with insulin‐like growth factor 1 (IGF‐1) treatment. …”
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Involvement of Protease-Activated Receptor2 Pleckstrin Homology Binding Domain in Ovarian Cancer: Expression in Fallopian Tubes and Drug Design by Jeetendra Kumar Nag, Sorina Grisaru-Granovsky, Shunit Armon, Tatyana Rudina, Priyanga Appasamy, Rachel Bar-Shavit

“…PAR2 expression in FTs may serve as an early prediction sensor for ovarian cancer. We show now that knocking down Par2 inhibits ovarian cancer peritoneal dissemination in vivo, pointing to the central role of PAR2. …”
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A search for new molecular targets for optimizing plague preventive vaccination and therapy by E. A. Krasil'nikova, A. S. Trunyakova, A. S. Vagaiskaya, T. E. Svetoch, R. Z. Shaikhutdinova, S. V. Dentovskaya

“…The development of the infectious process in susceptible host is accounted for by a whole set of pathogenicity factors in plague pathogen displaying various functional modalities being expressed depending on stage of infectious process, providing their coordinated expression. Knocking out any of such factors, in turn, may not either affect microbe virulence or lead to its attenuation. …”
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CD44 Depletion in Glioblastoma Cells Suppresses Growth and Stemness and Induces Senescence by Constantinos Kolliopoulos, Mohamad Moustafa Ali, Casimiro Castillejo-Lopez, Carl-Henrik Heldin, Paraskevi Heldin

“…Here, we sought to elucidate the molecular mechanisms by which CD44 promotes GBM progression by knocking out (KO) CD44, employing CRISPR/Cas9 gene editing in U251MG cells. …”
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Enhancing endometrial receptivity: the roles of human chorionic gonadotropin in autophagy and apoptosis regulation in endometrial stromal cells by Bin Wang, Mingxia Gao, Ying Yao, Haofei Shen, Hongwei Li, Jingjing Sun, Liyan Wang, Xuehong Zhang

“…However, when small interfering RNA was used to knock down LHCGR, hCG was less capable of controlling endometrial receptivity and autophagy molecules in ESCs. …”
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Meta-Analysis of Caenorhabditis elegans Transcriptomics Implicates Hedgehog-Like Signaling in Host-Microbe Interactions by Alejandra Zárate-Potes, Irtiqa Ali, Margarida Ribeiro Camacho, Hayley Brownless, Alexandre Benedetto

“…To investigate this further, we knocked-down 96 individual HHLS genes by RNAi, using high-throughput assays to assess their impact on three worm-gut infection models (Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis) and two worm-commensal paradigms (Comamonas sp., and Bacillus subtilis). …”
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Parkin-dependent mitophagy occurs via proteasome-dependent steps sequentially targeting separate mitochondrial sub-compartments for autophagy by Anna Lechado-Terradas, Sandra Schepers, Katharina I. Zittlau, Karan Sharma, Orkun Ok, Julia C. Fitzgerald, Stefan Geimer, Benedikt Westermann, Boris Macek, Philipp J. Kahle

“…Abbreviations: BafA, Bafilomycin A; CCCP, carbonyl cyanide 3-chlorophenylhydrazone; COX IV, cytochrome c oxidase subunit IV; CS, citrate synthase; DMEM, Dulbecco’s modified Eagle’s medium; EGFP, enhanced green fluorescent protein; FBS, fetal bovine serum; IF, immunofluorescence; IMM, inner mitochondrial membrane; KO, knock-out; LC3, microtubule-associated protein 1 light chain 3; MDVs, mitochondria-derived vesicles; MFN, mitofusin; NPCs, neural progenitor cells; OMM, outer mitochondrial membrane; p62/SQSTM, 62kDa protein sequestosome-1; PBS, phosphate-buffered saline; PINK1, phosphatase and tensin homolog (PTEN)-induced putative kinase protein 1; RT, room temperature; SSBP1, single-stranded DNA binding protein 1; TAX1BP1, Tax1-binding protein 1; TEM, transmission electron microscopy, TOM20, translocase of outer mitochondrial membrane 20kDa subunit; TOM70, translocase of outer mitochondrial membrane 70kDa subunit; Ub, ubiquitin; UPS, ubiquitin proteasome system; VDAC, voltage-dependent anion-selective channel protein; WB, Western blot; WT, wild-type…”
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Comprehensive bioinformatics analysis reveals the role of cuproptosis-related gene Ube2d3 in myocardial infarction by Ming Yang, Yucheng Wang, Liming He, Xinxin Shi, Shuwei Huang

“…In order to further clarify the role of Ube2d3, we knocked down Ube2d3 expression in OGD-treated AC16 cells, and confirmed Ube2d3’s promoting role in the hypoxia damage of AC16 cells by inducing cuproptosis, as evidenced by the detection of MTT, TUNEL, LDH release and cuproptosis-related proteins.ConclusionIn summary, our findings indicate that Ube2d3 regulates cuproptosis to affect the progression of MI.…”
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Female sex protects against renal edema, but not lung edema, in mice with partial deletion of the endothelial barrier regulator Tie2 compared to male sex. by Anoek L I van Leeuwen, Elise Beijer, Roselique Ibelings, Nicole A M Dekker, Marjolein R A van der Steen, Joris J T H Roelofs, Matijs van Meurs, Grietje Molema, Charissa E van den Brom

“…<h4>Conclusion</h4>Female sex seems to protect against renal, but not pulmonary edema in heterozygous Tie2 knock-out mice. This could not be explained by sex dimorphism in the endothelial angiopoietin/Tie2 system.…”
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Evaluation of Mycobacterium avium subsp. paratuberculosis isocitrate lyase (IcL) and ABC transporter (BacA) knockout mutants as vaccine candidates by Razieh Eshraghisamani, Rakel Arrazuria, Lucy Luo, Jeroen De Buck

“…There has been little success in controlling Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis, due to suboptimal diagnostics and the ineffectiveness of available vaccines. By knocking out BacA and IcL, genes required for MAP survival in dairy calves, two live-attenuated vaccine candidates were created. …”
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LncRNA LBX2-AS1 promotes proliferation and migratory capacity of clear cell renal cell carcinoma through mitophagy by Bao Wang, Yuang Wei, Tian Han, Peng Ji, Haoqi Miao, Xiangzheng Wu, Jian Qian, Pengfei Shao

“…Meanwhile, LBX2-AS1 knocking down dampened the proliferation of ccRCC cells in vivo.…”
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Differential vulnerability of hippocampal CA3-CA1 synapses to Aβ by Olivia A. Shipton, Clara S. Tang, Ole Paulsen, Mariana Vargas-Caballero

“…These results suggest that hippocampal CA3-CA1 synapses with presynaptic axon originating in the left CA3 are selectively vulnerable to Aβ and that a genetic knock out of tau protein protects them from Aβ synaptotoxicity.…”
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Extracellular vesicles from UTX-knockout endothelial cells boost neural stem cell differentiation in spinal cord injury by Yudong Liu, Zixiang Luo, Yong Xie, Yi Sun, Feifei Yuan, Liyuan Jiang, Hongbin Lu, Jianzhong Hu

“…Conversely, inhibiting Akt phosphorylation or knocking down L1CAM negated the beneficial effects of KO EVs on NSC neuronal differentiation. …”
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Pentraxin 3 regulates neutrophil infiltration to the brain during neuroinflammation [version 1; peer review: 2 approved] by Ivana Rajkovic, Eloise Lemarchand, Raymond Wong, Stuart M. Allan, Rory Tinker, Emmanuel Pinteaux

“…Methods: Neutrophil transmigration through interleukin-1β (IL-1β) activated brain endothelium and neurotoxicity of neutrophils isolated from wild-type (WT) or PTX3 knock-out (KO) mice was assessed in vitro. Primary cortical neuronal death after treatment with transmigrated neutrophils was quantified by lactate dehydrogenase (LDH) assay. …”
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Properdin Pattern Recognition on Proximal Tubular Cells Is Heparan Sulfate/Syndecan-1 but Not C3b Dependent and Can Be Blocked by Tick Protein Salp20 by Rosa G. M. Lammerts, Ditmer T. Talsma, Wendy A. Dam, Mohamed R. Daha, Marc A. J. Seelen, Stefan P. Berger, Jacob van den Born

“…Heparan sulfate proteoglycan dependency of the pattern recognition of properdin was evaluated on PTEC knocked down for syndecan-1 by shRNA technology. Solid phase binding assays were used to evaluate the effectivity of heparin(oids) and recombinant Salp20 to block the pattern recognition of properdin.Results: Binding of serum-derived and recombinant properdin preparations to PTECs could be dose-dependently inhibited (P &lt; 0.01) and competed off (P &lt; 0.01) by recombinant Salp20 (IC50: ~125 ng/ml) but not by Compstatin. …”
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Genome-Engineered mpkCCDc14 Cells as a New Resource for Studying AQP2 by Hyo-Ju Jang, Hye-Jeong Park, Hong Seok Choi, Hyun Jun Jung, Tae-Hwan Kwon

“…Cell proliferation rate and dDAVP-induced cAMP production were not affected by the knock-in of <i>Avpr2</i> and <i>Aqp2</i> genes. The AQP2 protein abundance was significantly higher in V2R-AQP2 cells compared with control mpkCCDc14 cells in the absence of dDAVP and the integrated AQP2 was detected. …”
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Study on Corrosion Behavior of High Temperature Distillation Crucible in LBE by WU Jiuwei;GUAN Guoying*;HUANG Xingde;LI Yong;GE Ding;WANG Yu;ZHAO Siqian

“…The crucible made of quartz material is cracked by slightly knocking, the outer surface is attached with gray material that is hard to be erased. …”
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Cuproptosis-related genes predict prognosis and trastuzumab therapeutic response in HER2-positive breast cancer by Rui Sha, Xinrui Dong, Shanshan Yan, Huijuan Dai, Aijun Sun, Liuxia You, Zongjin Guo

“…High expressed DLAT was associated with resistant to HER2-targeted therapy. Knocking down DLAT with siRNA increased sensitivity of breast cancer to trastuzumab. …”
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DNMTs Are Involved in TGF-β1-Induced Epithelial–Mesenchymal Transitions in Airway Epithelial Cells by Joo-Hoo Park, Jae-Min Shin, Hyun-Woo Yang, Il-Ho Park

“…DNMT1, DNMT3A, and DNMT3B gene expression were knocked down using siRNA transfection. MAPK phosphorylation and EMT-related transcription factor levels were determined using Western blotting. …”
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