Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i> by Jianxin Gao, Zhonghui Han, Ping Li, Hongyan Zhang, Xinjun Du, Shuo Wang

“…In addition, the variations in biofilm composition of <i>C. sakazakii</i> were examined using Raman spectroscopy analyses caused by knocking out <i>ompF</i>, and the result indicated that the levels of certain biofilm components, including lipopolysaccharide (LPS), were significantly decreased in the mutant (Δ<i>ompF</i>). …”
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Bacterial Outer Membrane Vesicles Promote Lung Inflammatory Responses and Macrophage Activation via Multi-Signaling Pathways by Sunhyo Ryu, Kareemah Ni, Chenghao Wang, Ayyanar Sivanantham, Jonathan M. Carnino, Hong-Long Ji, Yang Jin

“…Using mouse inflammation and autoimmune arrays, we identified multiple altered cytokine/chemokines in both bone marrow-derived macrophages and alveolar macrophages, suggesting that OMVs have a broader spectrum of function compared to LPS. Using TLR4 knock-out cells, we found that OMVs exert more robust effects on activating macrophages compared to LPS. …”
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Inhibition of ANGPT2 activates autophagy during hypertrophic scar formation via PI3K/AKT/mTOR pathway by Hongxin Chen, Kai Xu, Chao Sun, Si Gui, Juanjuan Wu, Song Wang

“…HSFs were transfected with sh-ANGPT2 to knock down ANGPT2 expression and then treated with MHT1485, the mTOR agonist. …”
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Loss of Nudt15 thiopurine detoxification increases direct DNA damage in hematopoietic stem cells by Noriaki Yamashita, Masahiro Kawahara, Takayuki Imai, Goichi Tatsumi, Ai Asai-Nishishita, Akira Andoh

“…Using previously established Nudt15 R138C knock-in mice, which mimic myelosuppression in NUDT15 R139C homozygous or heterozygous patients following thiopurine administration, we investigated the numerical changes of HSCs and hematopoietic progenitor cells following 6-MP administration using in vivo flowcytometry and ex vivo HSC expansion. …”
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Identification of tryptophan metabolism- and immune-related genes signature and prediction of immune infiltration landscape in bladder urothelial carcinoma by Guanwen Zhou, Guoliang Qin, Zhaocun Zhang, Haifeng Zhao, Linlin Xue

“…RT-qPCR revealed that NAMPT was upregulated in BLCA samples. Knocking down NAMPT significantly inhibited BLCA cell proliferation, migration, and invasion.DiscussionIn our study, we constructed a tryptophan metabolism- and immune-related gene risk model based on three biomarkers, namely NAMPT, IDO1, and ACAT1, that were significantly associated with the progression and immune landscape of BLCA. …”
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AQP8 promotes glioma proliferation and growth, possibly through the ROS/PTEN/AKT signaling pathway by Zhang Hao, Sheng Huajun, Guo Zhen, Xing Yu, Liu Qian, Cai Ziling, Shen Zihao, Xia Qingqian, Zhu Shujuan

“…Methods The dCas9-SAM and CRISPR/Cas9 techniques were used to construct viruses with overexpressed and knocked down AQP8, respectively, and infect A172 and U251 cell lines. …”
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Co-modulation of TNFR1 and TNFR2 in an animal model of multiple sclerosis by Timon Fiedler, Richard Fairless, Kira Pichi, Roman Fischer, Fabian Richter, Roland E. Kontermann, Klaus Pfizenmaier, Ricarda Diem, Sarah K. Williams

“…Methods Experimental autoimmune encephalomyelitis (EAE) was induced with myelin oligodendrocyte glycoprotein (MOG35-55) in humanized TNFR1 knock-in mice. These were treated with a human-specific TNFR1-selective antagonistic antibody (H398) and a mouse-specific TNFR2 agonist (EHD2-sc-mTNFR2), both in combination and individually. …”
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A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes. by Azad A Sulaiman, Katarzyna Zolnierczyk, Ornampai Japa, Jonathan P Owen, Ben C Maddison, Richard D Emes, Jane E Hodgkinson, Kevin C Gough, Robin J Flynn

“…Use of RNAi demonstrates that this effect is dependent on TGF-β RII and mRNA knock-down leads to a loss of IL-10 and PD-L1. Finally, we demonstrate that FhTLM aids newly excysted juveniles (NEJs) in their evasion of antibody-dependent cell cytotoxicity (ADCC) by reducing the NO response of macrophages-again dependent on TGF-β RI kinase. …”
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Long non-coding RNA LINC00565 regulates ADAM19 expression through sponging microRNA-532-3p, thereby facilitating clear cell renal cell carcinoma progression by Bin Meng, Pengfei Wang, Chaofei Zhao, Guangwei Yin, Xin Meng, Lin Li, Shengyong Cai, Chengquan Yan

“…Overexpressed ADAM19 could eliminate the anticancer effects caused by knocking down LINC00565 on ccRCC cells. In conclusion, LINC00565 upregulated ADAM19 via absorbing miR-532-3p, thereby facilitating the progression of ccRCC cells.…”
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The Prevalence of Postural Abnormalities in Rural Women Based on Age and Body Mass Index in Markazi Province, Iran by Reyhane Farahani, Ali Asghar Norasteh, Milad Fadaei Dehcheshmeh

“…According to the chi-square test results, the difference based on age was significant in forward head, hyperlordosis, knock knee, and pes cavus.Conclusion Th increase of age and BMI can impair postural control in rural women. …”
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T_h17, T_h22, and Myeloid-Derived Suppressor Cell Population Dynamics and Response to IL-6 in 4T1 Mammary Carcinoma by Viva J. Rasé, Reid Hayward, James M. Haughian, Nicholas A. Pullen

“…To further address the mechanism underlying type 3 immune cell and MDSC recruitment, we used CRISPR-Cas9 to knock out 4T1 tumor production of interleukin-6 (4T1-IL-6-KO), which functions in myelopoiesis, MDSC recruitment, and T_h maturation. …”
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COL10A1 promotes tumorigenesis by modulating CD276 in pancreatic adenocarcinoma by Qiaodong Xu, Jieting Zheng, Zegeng Su, Binlie Chen, Songgang Gu

“…Differentially expressed genes that were related to COL10A1 in PAAD were analysed via the LinkedOmics portal. After COL10A1 was knocked down, CD276 expression was assessed by western blotting. …”
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…Methods. miR-182-5p and fibroblast growth factor 9 (FGF9) were overexpressed or knocked down in IL-1β-induced chondrocytes. An OA knee model was performed by surgically destroying the medial meniscus. …”
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Development and Application of Ion Current/Cylinder Pressure Cooperative Combustion Diagnosis and Control System by Denghao Zhu, Jun Deng, Jinqiu Wang, Shuo Wang, Hongyu Zhang, Jakob Andert, Liguang Li

“…The application of advanced technologies for engine efficiency improvement and emissions reduction also increase the occurrence possibility of abnormal combustions such as incomplete combustion, misfire, knock or pre-ignition. Novel promising combustion modes, which are basically dominated by chemical reaction kinetics show a major difficulty in combustion control. …”
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PNPLA3 148M/M Is More Susceptible to Palmitic Acid-Induced Endoplasmic Reticulum Stress-Associated Apoptosis in HepG2 Cells by Yunzhi Chen, Xuemei Yan, Tian Wang, Hongrong Deng, Xiaojie Deng, Fen Xu, Hua Liang

“…Methods. A PNPLA3148I knock-in HepG2 cell model was constructed based on HepG2 expressing PNPLA3 148M/M using the Cas9/sgRNA system. …”
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miR-1297 sensitizes glioma cells to temozolomide (TMZ) treatment through targeting adrenomedullin (ADM) by Zongze He, Meixiong Cheng, Junting Hu, Lingtong Liu, Ping Liu, Longyi Chen, Deqian Cao, Jian Tang

“…Results ADM expression has been found upregulated in TMZ -resistant glioma samples based on bioinformatics and experimental analyses. Knocking down ADM in glioma cells enhanced the suppressive effects of TMZ on glioma cell viability, promotive effects on cell apoptosis, and inhibitory effects on mitochondrial membrane potential. …”
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Long Non-Coding RNA RP11-789C1.1 Suppresses Epithelial to Mesenchymal Transition in Gastric Cancer Through the RP11-789C1.1/MiR-5003/E-Cadherin Axis by Zehong Chen, Jialin Wu, Wensheng Huang, Jianjun Peng, Jinning Ye, Liang Yang, YuJie Yuan, Chuangqi Chen, Changhua Zhang, Shirong Cai, Yulong He, Suijing Wu, Wu Song

“…Furthermore, at both the mRNA and protein level, silencing RP11-789C1.1 remarkably reduced the expression of E-cadherin and promoted EMT, which were reversed by knocking down miR-5003. Conclusions: LncRNA RP11-789C1.1 inhibited EMT in GC through the RP11-789C1.1/miR-5003/E-cadherin axis, which could be a promising therapeutic target for GC.…”
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Cytosolic HMGB1 Mediates LPS-Induced Autophagy in Microglia by Interacting with NOD2 and Suppresses Its Proinflammatory Function by Seung-Woo Kim, Sang-A Oh, Song-I Seol, Dashdulam Davaanyam, Ja-Kyeong Lee

“…The LPS-mediated autophagy induction was significantly suppressed in BV2 cells after HMGB1 or NOD2 knock-down (KD), indicating that HMGB1 contributes to NOD2-mediated autophagy induction in microglia. …”
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Optimizing Hexose Utilization Pathways of <i>Cupriavidus necator</i> for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions by Lei Wang, Huiying Luo, Bin Yao, Junhu Yao, Jie Zhang

“…To explore the effect of the <i>pfkA</i> gene on the production of <i>C. necator</i>, an alanine-producing <i>C. necator</i> was constructed by expressing the NADPH-dependent L-alanine dehydrogenase, alanine exporter, and knocking out the <i>phaC1AB1</i> operon. The alanine-producing strain had maximum alanine titer and yield of 784 mg/L and 11.0%, respectively. …”
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Multiplexed <i>tat</i>-Targeting CRISPR-Cas9 Protects T Cells from Acute HIV-1 Infection with Inhibition of Viral Escape by Youdiil Ophinni, Sayaka Miki, Yoshitake Hayashi, Masanori Kameoka

“…HIV-1 cure strategy by means of proviral knock-out using CRISPR-Cas9 has been hampered by the emergence of viral resistance against the targeting guide RNA (gRNA). …”
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