Characterization of Putative Sporulation and Germination Genes in <i>Clostridium perfringens</i> Food-Poisoning Strain SM101 by Prabhat K. Talukdar, Mahfuzur R. Sarker

“…The β-glucuronidase reporter assay revealed that promoters of six out of eight tested genes (i.e., <i>bkdR</i>, <i>ylmC</i>, <i>ytaF</i>, <i>ytxC</i>, <i>yyaC1</i>, and <i>yyaC2</i>) were expressed only during sporulation, but not vegetative growth, suggesting that these genes are sporulation-specific. Gene knock-out studies demonstrated that <i>C. perfringens</i> Δ<i>bkdR</i>, Δ<i>ylmC</i>, Δ<i>ytxC</i>, and Δ<i>yyaC1</i> mutant strains produced a significantly lower number of spores compared to the wild-type strain. …”
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The Chemokine Receptor CXCR3 Isoform B Drives Breast Cancer Stem Cells by Namita Kundu, Xinrong Ma, Regine Brox, Xiaoxuan Fan, Tyler Kochel, Jocelyn Reader, Nuska Tschammer, Amy Fulton

“…Modulation of CXCR3B levels by knock in strategies increases CSC populations identified by aldehyde dehydrogenase activity or CD44 + CD24 − phenotype as well as tumorsphere-forming capacity. …”
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Golgi Alpha1,2-Mannosidase IA Promotes Efficient Endoplasmic Reticulum-Associated Degradation of NKCC2 by Sylvie Demaretz, Elie Seaayfan, Dalal Bakhos-Douaihy, Nadia Frachon, Martin Kömhoff, Kamel Laghmani

“…ManIA coexpression decreased total NKCC2 protein abundance whereas ManIA knock-down produced the opposite effect. Importantly, ManIA coexpression had a more profound effect on NKCC2 folding mutants. …”
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Unequal weakening of urbanization and soil salinization on vegetation production capacity by Qingwei Zhuang, Zhenfeng Shao, Deren Li, Xiao Huang, Bowen Cai, Orhan Altan, Shixin Wu

“…Changes in the NPP of FES have knock-on effects on food security, sustainable agricultural development, carbon sequestration, and environmental changes. …”
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Plasmodium falciparum contains functional SCF and CRL4 ubiquitin E3 ligases, and CRL4 is critical for cell division and membrane integrity. by Zeba Rizvi, G Srinivas Reddy, Somesh M Gorde, Priyanka Pundir, Divya Das, Puran Singh Sijwali

“…Immunoprecipitation, sequence analysis, and ubiquitination assay indicated that PfCullin-2 forms a functional human CRL4-like complex (PfCRL4), consisting of PfRbx1, cleavage and polyadenylation specificity factor subunit_A and WD40 repeat proteins. PfCullin-2 knock-down at the protein level, which would hinder PfCRL4 assembly, significantly decreased asexual and sexual erythrocytic stage development. …”
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Vertebral osteomyelitis caused by the novel pathogen Cutibacterium modestum: a case report by Hirokazu Toyoshima, Kaori Tanaka, Motoaki Tanigawa, Naoto Masuda, Chiaki Ishiguro, Hiroyuki Tanaka, Yuki Nakanishi, Shigetoshi Sakabe

“…Physical examination indicated knocking pain at the L4–L5 levels; magnetic resonance imaging showed irregular bone destruction of the L4 vertebral body, and low T1 and high T2 intensity lesions at the L4–L5 intervertebral disc. …”
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Animal Models of Metabolic Epilepsy and Epilepsy Associated Metabolic Dysfunction: A Systematic Review by Uday Praful Kundap, Yam Nath Paudel, Mohd. Farooq Shaikh

“…The result describes available models of metabolic dysfunction associated with epileptic disorder, such as mitochondrial respiration deficits, Lafora disease (LD) model-altered glycogen metabolism, causing epilepsy, glucose transporter 1 (GLUT1) deficiency, adiponectin responsive seizures, phospholipid dysfunction, glutaric aciduria, mitochondrial disorders, pyruvate dehydrogenase (PDH) α-subunit gene (PDHA1), pyridoxine dependent epilepsy (PDE), BCL2-associated agonist of cell death (BAD), Kcna1 knock out (KO), and long noncoding RNAs (lncRNA) cancer susceptibility candidate 2 (lncRNA CASC2). …”
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Role of <i>Chitin Deacetylase 1</i> in the Molting and Metamorphosis of the Cigarette Beetle <i>Lasioderma serricorne</i> by Wen-Jia Yang, Kang-Kang Xu, Yi Yan, Can Li, Dao-Chao Jin

“…The expression of <i>LsCDA1</i> was induced by 20-hydroxyecdysone (20E) in vivo, and it was significantly suppressed by knocking down the expression of ecdysteroidogenesis genes and 20E signaling genes. …”
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Data pipelines in a public health emergency: The human in the machine by Katy A.M. Gaythorpe, Rich G. Fitzjohn, Wes Hinsley, Natsuko Imai, Edward S. Knock, Pablo N. Perez Guzman, Bimandra Djaafara, Keith Fraser, Marc Baguelin, Neil M. Ferguson

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NOVA1 promotes SMN2 exon 7 splicing by binding the UCAC motif and increases SMN protein expression by Li-Li Du, Jun-Jie Sun, Zhi-Heng Chen, Yi-Xiang Shao, Liu-Cheng Wu

“…We also found that in vitro expression of NOVA1 increased the inclusion of SMN2 exon 7 and expression of the SMN2 protein in the U87MG cell line, whereas the opposite was observed when NOVA1 was knocked down. Finally, point mutation and RNA pull-down showed that the UCAC motif in SMN2 exon 7 plays a critical role in NOVA1 binding and promoting the inclusion of exon 7. …”
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NEK2 promotes esophageal squamous cell carcinoma cell proliferation, migration and invasion through the Wnt/β-catenin signaling pathway by Dong Guo, Weinan Yao, Xingyu Du, Jing Dong, Xueyuan Zhang, Wenbin Shen, Shuchai Zhu

“…The quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting (WB) analyses were performed to determine the expression levels of NEK2 mRNA and protein, respectively. We knocked down the NEK2 expression in ESCC cells (ECA109 and TE1), and evaluated the NEK2 biology function associated with ESCC cell proliferation, migration, invasion, and colony formation abilities. …”
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LncRNA HOTTIP impacts the proliferation and differentiation of fibroblast-like synoviocytes in ankylosing spondylitis through the microRNA-30b-3p/PGK1 axis by Li Wei, Xin Zhang, Yu Yao, Weizhuo Zheng, Jun Tian

“…Silencing miR-30b-3p or overexpressing PGK1 reversed the improvement of AS by knocking down HOTTIP or up-regulating miR-30b-3p. Conclusion Our study suggests that reduced HOTTIP ameliorates AS progression by suppressing the proliferation and differentiation of ASFLSs through the interaction of miR-30b-3p and PGK1.…”
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LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p by Yuexia Wang, Yue Wang, Xue Wang, Yuan Ma, Zhaojin Li, Yu Di

“…The degree of retinopathy was evaluated by staining retinal sections with hematoxylin and eosin, and the expression of CCN1, HIF-1α, VEGF, caspase-3, Bcl-2, IL-1β, and TNF-α protein was analyzed by Western blotting and immunohistochemistry.ResultsIn the retina tissue of OIR mice, TUG1, miR-145-5p, and CCN1 were differentially expressed. Knocking down TUG1 attenuated apoptosis, migration, and angiogenesis induced by hypoxia on HRECs, as did miR-145-5p overexpression. …”
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Knockdown of DNA methyltransferase 1 reduces DNA methylation and alters expression patterns of cardiac genes in embryonic cardiomyocytes by Xiefan Fang, Ryan Poulsen, Lu Zhao, Jingjing Wang, Scott A. Rivkees, Christopher C. Wendler

“…It is known that DNMT1 is essential for embryonic development, during which it is involved in regulating cardiomyocyte DNA methylation and gene expression. We used siRNA to knock down DNMT1 expression in primary cultures of mouse embryonic cardiomyocytes. …”
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Rap1a Overlaps the AGE/RAGE Signaling Cascade to Alter Expression of α-SMA, p-NF-κB, and p-PKC-ζ in Cardiac Fibroblasts Isolated from Type 2 Diabetic Mice by Stephanie D. Burr, James A. Stewart

“…Moreover, Rap1a, in conjunction with activation of the AGE/RAGE cascade, increased NF-κB expression as well as hydrogen peroxide concentration, indicating a possible oxidative stress response. Additionally, knocking down Rap1a expression resulted in an increase in SOD-1 expression suggesting that Rap1a can affect oxidative stress markers independently of the AGE/RAGE signaling cascade. …”
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High Thioredoxin Domain-Containing Protein 11 Expression Is Associated with Tumour Progression in Glioma by Ying-Tso Chen, Chia-Li Chung, Yu-Wen Cheng, Chien-Ju Lin, Tzu-Ting Tseng, Shu-Shong Hsu, Hung-Pei Tsai, Aij-Lie Kwan

“…Further, TXNDC11 knockdown downregulated N-cadherin and cyclin D1 expression and upregulated E-cadherin expression in GBM cells. Knock-in TXNDC11 return these. Finally, in vivo, orthotopic xenotransplantation of TXNDC11-silenced GBM cells into nude rats promoted slower tumour growth and prolonged survival time. …”
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In Vitro Evaluation of CD276-CAR NK-92 Functionality, Migration and Invasion Potential in the Presence of Immune Inhibitory Factors of the Tumor Microenvironment by Stefan Grote, Guillermo Ureña-Bailén, Kenneth Chun-Ho Chan, Caroline Baden, Markus Mezger, Rupert Handgretinger, Sabine Schleicher

“…Results: CD276-CAR NK-92 cells induced specific cytolysis of melanoma cell lines while being able to overcome a variety of the immunosuppressive effects normally exerted by the TME. NKG2A knock-out did not further improve CAR NK-92 cell-mediated cytotoxicity. …”
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RAD51 is a poor prognostic marker and a potential therapeutic target for oral squamous cell carcinoma by Yu-Fen Tsai, Leong-Perng Chan, Yuk-Kwan Chen, Chang-Wei Su, Ching-Wei Hsu, Yen-Yun Wang, Shyng-Shiou F. Yuan

“…Lentiviral infection was used to knock down or overexpress RAD51. The influence of RAD51 on the biological profile of oral cancer cells was evaluated. …”
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Long non-coding RNA TTN antisense RNA 1 facilitates hepatocellular carcinoma progression via regulating miR-139-5p/SPOCK1 axis by Xinghao Zhu, Shiqing Jiang, Zongyao Wu, Tonghua Liu, Wei Zhang, Lili Wu, Lijun Xu, Mingliang Shao

“…After TTN-AS1 was overexpressed or knocked down in HCC cells, CCK-8 and 5-Ethynyl-2ʹ-deoxyuridine (EdU) assays were carried out for examining cell multiplication. …”
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Knockout of the Transducin-Like Enhancer of Split 6 Gene Affects the Proliferation and Cell Cycle Process of Mouse Spermatogonia by Meiying Feng, Yinshan Bai, Yun Chen, Kai Wang

“…The results showed that, after drug induction, the <i>Tle6</i> gene in mouse spermatogonia was successfully knocked out at the genome and protein levels, and the <i>Tle6</i> gene knockout efficiency was confirmed to be 87.5% with gene-cloning technology. …”
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