S. aureus virulence factors decrease epithelial barrier function and increase susceptibility to viral infection by Mary C. Moran, Matthew G. Brewer, Patrick M. Schlievert, Lisa A. Beck

“…CRISPR/Cas9 was used to knock out CD40, a potential enterotoxin receptor on epithelial cells. …”
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Outer Membrane Porin F in E. coli Is Critical for Effective Predation by Bdellovibrio by Wonsik Mun, Sumudu Upatissa, Sungbin Lim, Mohammed Dwidar, Robert J. Mitchell

“…Furthermore, recognizing OmpF as a prey surface structure was dependent on the prey strain, as knocking out OmpF protein homologues in other prey species, including Escherichia fergusonii, Klebsiella pneumoniae, and Salmonella enterica, did not always reduce the predation rate. …”
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Amyloid fibril proteomics of AD brains reveals modifiers of aggregation and toxicity by Arun Upadhyay, Deepak Chhangani, Nalini R. Rao, Julia Kofler, Robert Vassar, Diego E. Rincon-Limas, Jeffrey N. Savas

“…Lastly, we used a transgenic Aβ42 fly model to test if knock down or over-expression of fibril-interacting gene homologues modifies neurotoxicity. …”
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Comparative Analysis of the APOL1 Variants in the Genetic Landscape of Renal Carcinoma Cells by Maty Tzukerman, Yeela Shamai, Ifat Abramovich, Eyal Gottlieb, Sara Selig, Karl Skorecki

“…Here, we demonstrate that knocking in the G1 or G2 APOL1 alleles, or complete elimination of APOL1 expression, has major effects on proliferation capacity, mitochondrial morphology, cell metabolism, autophagy levels, and the tumorigenic potential of RCC cells. …”
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Task sharing and stepped referral model for community mental health promotion in low- and middle-income countries (LMIC): insights from a feasibility study in India by Saju Madavanakadu Devassy, Lorane Scaria, Natania Cheguvera

“…Methods We tested the model using a cross-sectional, one-phase door-knock survey in three geographically defined locations in Kerala, India, between May and July 2019. …”
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Identification of 10 genes on Candida albicans chromosome 5 that control surface exposure of the immunogenic cell wall epitope β-glucan and cell wall remodeling in caspofungin-adap... by Sudisht K. Sah, Anshuman Yadav, Michael D. Kruppa, Elena Rustchenko

“…We identified 10 genes on C. albicans chromosome 5 (Ch5) that are simultaneously downregulated to decrease susceptibility to ECN caspofungin. Independent knock-out experiments demonstrated that these genes act overall to decrease the 1,3-β-glucan level in the cell wall, change levels of mannan and chitin, and affect expression of FKS genes. …”
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Downregulation of lncRNA SLC7A11-AS1 decreased the NRF2/SLC7A11 expression and inhibited the progression of colorectal cancer cells by Tian Wang, Si Liang, Yajing Li, Xiyu Wang, Hongjie Wang, Jiguang Guo, Ming Li

“…And the down regulated expression of SLC7A11 and lower ROS level causing by SLC7A11-AS1 knocked down could be relieved by overexpressed NRF2. …”
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KLF6 alleviates hepatic ischemia-reperfusion injury by inhibiting autophagy by Jiye Li, Dongsheng Yu, Chenhui He, Qiwen Yu, Zhongkun Huo, Yi Zhang, Shuijun Zhang

“…KLF6 deficiency markedly exacerbated liver damage, cell apoptosis, and activation of hepatic inflammatory responses, whereas hepatic overexpression of KLF6 in mice produced the opposite results. In addition, we knocked out or overexpressed KLF6 in AML12 cells before exposing them to a hypoxia-reoxygenation challenge. …”
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IGF2BP2 acts as a m6A modification regulator in laryngeal squamous cell carcinoma through facilitating CDK6 mRNA stabilization by Xiaojun Tang, Qinglai Tang, Shisheng Li, Mengmeng Li, Tao Yang

“…In the xenograft tumor model derived from IGF2BP2 knocked-down LSCC cells, IGF2BP2 knockdown inhibited tumor growth. …”
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Effect of shRNA Mediated Silencing of YB-1 Protein on the Expression of Matrix Collagenases in Malignant Melanoma Cell In Vitro by Wisam Nabeel Ibrahim, Abd Almonem Doolaanea, Mohammad Syaiful Bahari Bin Abdull Rasad

“…Thus, in this study, the aim was to knock down the expression of YB-1 in A375 malignant melanoma cancer cell using the shRNA approach and study its effect on cancer cell proliferation, migration, and expression of collagenases. …”
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Teicoplanin associated gene tcaA inactivation increases persister cell formation in Staphylococcus aureus by Gul Habib, Haji Gul, Haji Gul, Prevez Ahmad, Azam Hayat, Mujaddad Ur Rehman, Ihab Mohamed Moussa, Hosam O. Elansary

“…The RNA-seq data revealed a significant decrease in tcaA (p = 0.008) and tcaB (p = 0.04) expression while tcaR was not significantly altered. We knocked down tcaA, tcaB, and tcaR using CRISPR-dCas9 and the results showed that when tcaA was suppressed by dCas9, a significant increase was witnessed in persister cells while tcaB suppression did not induce persistence. …”
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BNC1 promotes cell migration and invasion of lung squamous cell carcinoma by ZHANG Longyao, ZENG Longjin, ZHENG Linpeng

“…Screening of BNC1 downstream regulatory molecules at the mRNA level showed that CDH1 was decreased when BNC1 was overexpressed (P < 0.05), and increased when BNC1 was knocked down (P < 0.05). The results of mRNA level were consistent with the results verified by Western blotting (P < 0.05). …”
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Evolution and Application of Genome Editing Techniques for Achieving Food and Nutritional Security by Sajid Fiaz, Sunny Ahmar, Sajjad Saeed, Aamir Riaz, Freddy Mora-Poblete, Ki-Hung Jung

“…The novel CRISPR-associated protein system 9 variants, namely BE and PE, can generate transgene-free plants with more frequency and are therefore being used for knocking out of genes of interest. We provide a comprehensive review of the evolution of genome editing technologies, especially the application of the third-generation genome editing technologies to achieve various plant breeding objectives within the regulatory regimes adopted by various countries. …”
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Effects of Functional Depletion of <i>Doublesex</i> on Male Development in the Sawfly, <i>Athalia rosae</i> by Shotaro Mine, Megumi Sumitani, Fugaku Aoki, Masatsugu Hatakeyama, Masataka G. Suzuki

“…In the present study, we knocked down the <i>A. rosae</i> ortholog of <i>dsx</i> (<i>Ardsx</i>) during several developmental stages with repeated double-stranded RNA (dsRNA) injections. …”
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Gut microbiota-related metabolite alpha-linolenic acid mitigates intestinal inflammation induced by oral infection with Toxoplasma gondii by Jing Yang, Songhao Liu, Qian Zhao, Xiaobing Li, Kangfeng Jiang

“…In our previous study, there was a decrease in the severity of toxoplasmosis after T. gondii α-amylase (α-AMY) was knocked out. Here, we established mouse models of ME49 and Δα-amy cyst infection and then took advantage of 16S rRNA gene sequencing and metabolomics analysis to identify specific gut microbiota-related metabolites that mitigate T. gondii-induced intestinal inflammation and analyzed the underlying mechanism. …”
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Targeted inactivation of Salmonella Agona metabolic genes by group II introns and in vivo assessment of pathogenicity and anti-tumour activity in mouse model by Chin Piaw Gwee, Chai Hoon Khoo, Swee Keong Yeap, Geok Chin Tan, Yoke Kqueen Cheah

“…Agona were successfully knocked out using a Targetron gene knockout system. …”
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Overexpression of NNMT in Glioma Aggravates Tumor Cell Progression: An Emerging Therapeutic Target by Wei Sun, Yongxiang Zou, Zheng Cai, Jinxiang Huang, Xinjie Hong, Qiang Liang, Weilin Jin

“…Additionally, NNMT was knocked down in two types of glioma cells, U87 and U251, to evaluate the invasive ability of these cells. …”
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Schistosome tegumental ecto-apyrase (SmATPDase1) degrades exogenous pro-inflammatory and pro-thrombotic nucleotides by Akram A. Da’dara, Rita Bhardwaj, Yasser B.M. Ali, Patrick J. Skelly

“…In this work we employ RNAi to knock down expression of the genes encoding these enzymes in the intravascular life stages of the parasite. …”
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Histone modifications induced by ethanol are modulated by genotype, brain region studied, and timing of ethanol exposure by Andrey eFinegersh, Carolyn eFerguson, Seth eMaxwell, David eMazariegos, Daniel eFarrell, Gregg E Homanics

“…We hypothesized that chronic intermittent EtOH (CIE) induces persistent changes in histone modifications across the cerebral cortex (CCx), nucleus accumbens (NAc), and prefrontal cortex (PFC), and that these histone modifications are altered in a knock-in mouse strain with altered sensitivity to EtOH. …”
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Development of a Control-Oriented Ignition Delay Model for GCI Combustion by Giacomo Silvagni, Vittorio Ravaglioli, Stefania Falfari, Fabrizio Ponti, Valerio Mariani

“…Since the combustion of the pre-injections is chemically driven, the ignition delay might be strongly affected by a slight variation in the engine control parameters and, consequently, lead to misfire or knocking. The goal of this work was to develop a control-oriented ignition delay model suitable to improve the GCI combustion stability through the proper management of the pilot injections. …”
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