Different Ectopic Hoxa2 Expression Levels in Mouse Cranial Neural Crest Cells Result in Distinct Craniofacial Anomalies and Homeotic Phenotypes by Taro Kitazawa, Maryline Minoux, Sebastien Ducret, Filippo M. Rijli

“…To address this issue, we selectively induced <i>Hoxa2</i> overexpression in mouse CNCCs, using a panel of mouse lines expressing different <i>Hoxa2</i> ectopic expression levels, including a newly generated <i>Hoxa2</i> knocked-in mouse line. While ectopic <i>Hoxa2</i> expression at only 60% of its physiological levels was sufficient for pinna duplication, ectopic <i>Hoxa2</i> expression at 100% of its normal level was required for complete homeotic repatterning of a subset of PA1 skeletal elements into a duplicated set of PA2-like elements. …”
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Niclosamide improves cancer immunotherapy by modulating RNA-binding protein HuR-mediated PD-L1 signaling by Qi Zhang, Zhe Yang, Xinbao Hao, Lauren J. Dandreo, Lily He, Yuxia Zhang, Fen Wang, Xiaoqing Wu, Liang Xu

“…Results We found that HuR directly bound to and stabilized PD-L1 mRNA. Knocking out HuR reduced PD-L1 levels and promoted T cell activation. …”
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Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b by Wei Wang, Mei Wang, Jing Xu, Fei Long, Xianbao Zhan

“…TUNEL staining results showed that knocking down or overexpression of GATA3 did not cause significant changes to apoptotic bodies of CRC cells, while oxaliplatin treatment increased the number of apoptotic bodies. …”
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Transcriptomic Effects of LINC01614 on Lung Cancer A549 Cells and Relevance of Drug Resistance by BAI Yuming, LI Jin, SHI Lin, JIA Yongfeng, LIU Xia, YUN Fen

“…Transcriptome sequencing was performed on A549 cells knocked down with LINC01614. We validated the transcriptomic differential genes MCAM and ABCC3 at the gene level and MCAM at the protein level, detected the IC50 changes of A549 cells after knockdown of LINC01614 under the effect of different concentrations of cisplatin, and detected the effect of knockdown of LINC01614 on the migration ability of A549 cells. …”
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IGF2 deficiency promotes liver aging through mitochondrial dysfunction and upregulated CEBPB signaling in d-galactose-induced aging mice by Xiaohai Zhou, Bowen Tan, Weiwei Gui, Caiping Zhou, Hanxin Zhao, Xihua Lin, Hong Li

“…In vitro, we used specific short hairpin RNA against IGF2 to knock down IGF2 in AML12 cells. d-galactose and hydrogen peroxide treatment were used to induce AML12 cell senescence. …”
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RETRACTED ARTICLE: Down-regulation of kappa opioid receptor promotes ESCC proliferation, invasion and metastasis via the PDK1-AKT signaling pathway by Han-Ming Huang, Xin-Hua He, Xiao-Yu Huang, Guo-Yun Wang, Qiao-Xi Xia, Ze-Peng Du, Yong-Fa Zhang

“…This study focused on exploring the effect of knocking down KOR in ESCC and its underlying mechanism. …”
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Sestrin1 is involved in the regulation of gluconeogenesis in mouse liver cells by GUO Yanfang, GENG Chao, XIE Xianghong, CHEN Enhui, GUO Zeyu, ZHANG Minglong, LIU Xiaojun

“…The effect of SESN1 on PGC-1α deacetylation was detected by overexpression of SESN1 and inhibition of SIRT1 expression.By knocking down SIRT1 expression, we detected whether it mediated the changes in mRNA levels of SESN1 induced gluconeogenesis related genes. …”
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High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma by Shanchun Tao, Di Cui, Huimin Cheng, Xiaofei Liu, Zhaobin Jiang, Hongwei Chen, Yong Gao

“…Functional experiments confirmed that knocking down TBRG4 could inhibit the proliferation, migration, and invasion of HCC cells. …”
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The meaning of ubiquitylation of the DSL ligand Delta for the development of Drosophila by Tobias Troost, Ekaterina Seib, Alina Airich, Nicole Vüllings, Aleksandar Necakov, Stefano De Renzis, Thomas Klein

“…Results To clarify these issues, we generated and analysed Dl attP -DlK2R-HA, a knock-in allele into the Dl locus. Our analysis of this allele reveals that the sole presence of one copy of Dl attP -DlK2R-HA can provide sufficient activity for completion of development. …”
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PM21-particle stimulation augmented with cytokines enhances NK cell expansion and confers memory-like characteristics with enhanced survival by Jeremiah L. Oyer, Tayler J. Croom-Perez, Md Faqrul Hasan, Javier A. Rivera-Huertas, Sarah B. Gitto, Joanna M. Mucha, Xiang Zhu, Deborah A. Altomare, Robert Y. Igarashi, Alicja J. Copik

“…Finally, CAP-NK cells were amenable to current technologies used in developing therapeutic NK cell products, including CRISPR/Cas9-based techniques to generate a triple-gene knockout or a gene knock-in. Taken together, these data demonstrate that the addition of cytokines enhanced the already effective method of ex vivo generation of therapeutic NK cells with PM21-particles, yielding a superior NK cell product for manufacturing efficiency and potential therapeutic applications.…”
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Exploring the Functional Roles of Telomere Maintenance 2 in the Tumorigenesis of Glioblastoma Multiforme and Drug Responsiveness to Temozolomide by Shao-Wei Feng, Zih-Syuan Wu, Yi-Lin Chiu, Shih-Ming Huang

“…Hence, it is urgent to address the functional role of TELO2 in the tumorigenesis and TMZ treatment of GBM. In this study, we knocked down <i>TELO2</i> mRNA in GBM8401 cells, a grade IV GBM, compared with <i>TELO2</i> mRNA overexpression in human embryonic glial SVG p12 cells and normal human astrocyte (NHA) cells. …”
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The Case for TAAR1 as a Modulator of Central Nervous System Function by Grazia Rutigliano, Grazia Rutigliano, Alice Accorroni, Alice Accorroni, Riccardo Zucchi

“…However each of these substances may have additional molecular targets, and it is unclear whether their endogenous levels are sufficient to produce significant TAAR1 activation in vivo. TAAR1 knock out mice show a worse performance in anxiety and working memory tests, and they are more prone to develop ethanol addiction. …”
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Mismatch repair gene MSH6 correlates with the prognosis, immune status and immune checkpoint inhibitors response of endometrial cancer by Lin-Zhi Zhou, Hong-Qi Xiao, Jie Chen

“…In vitro experiments also confirmed that after knocking down MSH6 in endometrial cancer cells, their proliferation, migration and invasion abilities were weakened, while the expression levels of PD-L1 and PD-L2 were elevated. …”
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Role of &#x003b2;-arrestin2 in colitis by regulating autophagy by Guo Jiaxiang, Zhai Xiaoming, Liu Huiling, Xu Minyi, Wu Shuyun, Tao Jin

“…And the expression levels of autophagy-related proteins LC3B-&#x02161;/&#x02160; were significantly down-regulated in &#x003b2;-arrestin2 KO mice in the experimental groups（P &lt; 0.05）.There is no significant difference in the expression levels of Beclin1（P &gt; 0.05）. After knocking down the expression of &#x003b2;-arrestin2 in HCoEpiC cells and EBSS treatment, the expression levels of LC3B-&#x02161;/&#x02160; were significantly down-regulated （P &lt; 0.05）. …”
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Evaluation of ammonia-gasoline co-combustion in a modern spark ignition research engine by A. Ambalakatte, S. Geng, A. Cairns, A. Harrington, J. Hall, M. Bassett

“…Under all conditions, the indicated thermal efficiency of the engine was either equivalent to or slightly higher than that obtained using gasoline-only due to the favourable anti-knock rating of NH3. Ongoing work is concerned with detailed breakdown of individual NOx species together with measuring the impact of hydrogen enrichment across the operating map.…”
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Genome reduction in Paenibacillus polymyxa DSM 365 for chassis development by Giulia Ravagnan, Janne Lesemann, Moritz-Fabian Müller, Anja Poehlein, Rolf Daniel, Stephan Noack, Johannes Kabisch, Jochen Schmid

“…In the first stage, single knock-out mutants of non-essential genomic regions were created and evaluated for their biological fitness. …”
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The Cytoplasmic Dynein Associated Protein NDE1 Regulates Osteoclastogenesis by Modulating M-CSF and RANKL Signaling Pathways by Bhaba K. Das, Jyoti Gogoi, Aarthi Kannan, Ling Gao, Weirong Xing, Subburaman Mohan, Haibo Zhao

“…In this study, we found that knocking-down Nde1 expression by lentiviral transduction of specific shRNAs markedly inhibited osteoclastogenesis <i>in vitro</i> by attenuating the proliferation, survival, and differentiation of osteoclast precursor cells <i>via</i> suppression of signaling pathways downstream of M-CSF and RANKL as well as osteoclast differentiation transcription factor NFATc1. …”
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Differential effect of histone H3.3 depletion on retroviral repression in embryonic stem cells by Ayellet Tal, Jose David Aguilera, Igor Bren, Carmit Strauss, Sharon Schlesinger

“…Interestingly, we show that H3.3 depletion has a differential effect depending on which of the two genes coding for H3.3, H3f3a or H3f3b, are knocked out. Depletion of H3f3a resulted in a transient upregulation of incoming retroviral expression and ERVs, while the depletion of H3f3b did not have the same effect and repression was maintained. …”
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Analysis and Reconstitution of the Menaquinone Biosynthesis Pathway in <i>Lactiplantibacillus plantarum</i> and <i>Lentilactibacillus buchneri</i> by Nisit Watthanasakphuban, Ludovika Jessica Virginia, Dietmar Haltrich, Clemens Peterbauer

“…<i>Lactiplantibacillus plantarum</i> has only two genes (<i>menA</i>, <i>menG</i>) encoding enzymes in the biosynthetic pathway (out of eight), and <i>Lentilactobacillus buchneri</i> has only four (<i>menA</i>, <i>menB</i>, <i>menE</i>, and <i>menG</i>). We constructed knock-out strains of <i>L. lactis</i> defective in <i>menA</i>, <i>menB</i>, <i>menE</i>, and <i>menG</i> (encoding the last steps in the pathway) and complemented these by expression of the extant genes from <i>Lactipl. plantarum</i> and <i>Lent. buchneri</i> to verify their functionality. …”
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Transcriptomic Analysis of Age-Associated Periventricular Lesions Reveals Dysregulation of the Immune Response by Motaz M. Fadul, Paul R. Heath, Johnathan Cooper-Knock, Julian M. Kurz, Hayder A. Al-Azzawi, Zarki Ali, Taylor Smith, Fiona E. Matthews, Carol Brayne, Stephen B. Wharton, Julie E. Simpson

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