Regional contributions of D-serine to Alzheimer’s disease pathology in male AppNL–G–F/NL–G–F mice by Xiance Ni, Xiance Ni, Ran Inoue, Ran Inoue, Yi Wu, Yi Wu, Tomoyuki Yoshida, Tomoyuki Yoshida, Keisuke Yaku, Takashi Nakagawa, Takashi Nakagawa, Takashi Saito, Takashi Saito, Takaomi C. Saido, Keizo Takao, Keizo Takao, Keizo Takao, Hisashi Mori, Hisashi Mori, Hisashi Mori

“…In the mammalian brain, it is produced by serine racemase (SRR) from L-serine, suggesting that dysregulation of L-serine, D-serine, or SRR may contribute to AD pathogenesis.Objective and methodsWe examined the contributions of D-serine to AD pathology in the AppNL–G–F/NL–G–F gene knock-in (APPKI) mouse model of AD. We first examined brain SRR expression levels and neuropathology in APPKI mice and then assessed the effects of long-term D-serine supplementation in drinking water on neurodegeneration. …”
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Oligodendrogenesis and myelination tracing in a CRISPR/Cas9-engineered brain microphysiological system by July Carolina Romero, Cynthia Berlinicke, Sharon Chow, Yukan Duan, Yifei Wang, Xitiz Chamling, Lena Smirnova

“…While generating the PLP1-sfGFP reporter, we used reverse transfection and obtained higher Knock-In (KI) efficiency compared to forward transfection (61–72 vs. 46%).ResultsAfter validation of the KI and quality control of the PLP1-sfGFP line, selected clones were differentiated into bMPS, and the fidelity, specificity, and function of the tagged PLP protein were verified in this model. …”
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Intestinal Alkaline Phosphatase Prevents Sulfate Reducing Bacteria-Induced Increased Tight Junction Permeability by Inhibiting Snail Pathway by Sudha B. Singh, Cristina N. Coffman, Matthew G. Varga, Amanda Carroll-Portillo, Cody A. Braun, Henry C. Lin, Henry C. Lin

“…DSV-induced permeability effects were inhibited in cells knocked down for snail. Pre-treatment of cells with IAP inhibited DSV-induced FITC flux and snail expression and DSV-mediated disruption of occludin staining. …”
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Andrographolide alleviates paraquat-induced acute lung injury by activating the Nrf2/HO-1 pathway by Degang Zhang, Fengqin Shen, Shiting Ma, Suting Nan, Yanhong Ma, Lili Ren, Hao Li, Qin Yu

“…Nuclear factor erythroid like-2 (Nrf2) was knocked out to explore the specific effects of the Nrf2/ Heme oxygenase-1 (OH-1) pathway in the protection of Andro against ALI caused by PQ.Results: Andro significantly reduced lung damage and the ratio of Wet/Dry (W/D) weight, decreased MDA, IL-6, IL-1β, and TNF-ɑ levels, reversed the decrease of CAT and SOD levels, and inhibited apoptosis caused by PQ. …”
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A Physiogenomic Study of the Tolerance of <i>Saccharomyces cerevisiae</i> to Isoamyl Alcohol by Jialin Song, Yu Wang, Hengyuan Xu, Jinshang Liu, Jianping Wang, Haojun Zhang, Cong Nie

“…Strains with key genes knocked out were cultured without isoamyl alcohol. …”
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Loss function of NtGA3ox1 delays flowering through impairing gibberellins metabolite synthesis in Nicotiana tabacum by Lele Deng, Chaofan Li, Qian Gao, Wenwu Yang, Jiarui Jiang, Jiaxin Xing, Haiying Xiang, Jun Zhao, Yekun Yang, Pengfei Leng

“…In this study, we created a late flowering mutant HG14 through knocking out NtGA3ox1 by CRISPR/Cas9. It took around 13.0 and 12.1 days longer to budding and flowering compared to wild type Honghuadajinyuan. …”
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CBS-H2S axis preserves the intestinal barrier function by inhibiting COX-2 through sulfhydrating human antigen R in colitis by Shihao Guo, Zhihao Huang, Jing Zhu, Taohua Yue, Xin Wang, Yisheng Pan, Dingfang Bu, Yucun Liu, Pengyuan Wang, Shanwen Chen

“…The sulfhydrated HuR increased when CBS express normally or GYY4137 was administered. While after knocking kown CBS, the expression of COX-2 in mice colon increased significantly, and the sulfhydration level of HuR decreased. …”
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Programmed Death-Ligand 1 (PD-L1) Expression Is Induced by Insulin in Pancreatic Ductal Adenocarcinoma Cells Pointing to Its Role in Immune Checkpoint Control by Steffen M. Heckl, Franziska Mau, Anke Senftleben, Tina Daunke, Silje Beckinger, Samir Abdullazade, Stefan Schreiber, Christoph Röcken, Susanne Sebens, Heiner Schäfer

“…Treatment of the PDAC cell lines BxPc3, A818-6, and T3M4 with insulin increased PD-L1 expression in a time- and dose dependent fashion, as shown by Western blot and qPCR analysis. siRNA mediated knock-down showed that the effects of insulin on PD-L1 depend on the insulin and IGF receptors (InsR and IGFR, respectively). …”
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Long non-coding RNA PRR7-AS1 promotes osteosarcoma progression via binding RNF2 to transcriptionally suppress MTUS1 by Gu Chen-Xi, Xu Jin-Fu, Huang An-Quan, Yu Xiao, Wu Ying-Hui, Li Suo-Yuan, Shen Cong, Zou Tian-Ming, Shen Jun

“…Potential downstream RNF2 of PRR7-AS1 was identified and explored using RNA pulldown and RIP. The GTRD and KnockTF database were used to predict the downstream target gene, MTUS1, and ChIP-qPCR experiments were used to verify the working mechanismy. …”
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IGF2BP2 Promotes Epithelial to Mesenchymal Transition and Metastasis through Stabilizing HMGA1 mRNA in Gastric Cancer by Jun Ouyang, Junqing Li, Dongwei Li, Jianlong Jiang, Tengfei Hao, Yujian Xia, Xiaofang Lu, Changhua Zhang, Yulong He

“…In subsequent experiments, it was then verified that by directly interacting with HMGA1 mRNA, IGF2BP2 augmented its stability and thus increased its expression. The knocking down of IGF2BP2 could significantly decrease the migration and invasion of GC cells, which could be reversed by increasing HMGA1 expression. …”
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An extracellular humanized IFNAR immunocompetent mouse model for analyses of human interferon alpha and subtypes by Yumeng Li, Asha Ashuo, Menghan Hao, Yaming Li, Jianyu Ye, Jiangxia Liu, Ting Hua, Zhong Fang, Jianhua Li, Zhenghong Yuan, Jieliang Chen

“…To more fully characterize the actions of human IFN-α and its subtypes in vivo, a gene targeting strategy was employed to generate gene knock-in mice with extracellular-humanized IFNAR1/2 (IFNAR-hEC) in the C57BL/6N strain. …”
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Comprehensive analysis of clinical prognosis and biological significance of CNIH4 in cervical cancer by Jiajia Wang, Shudan Wang, Junli Wang, Jingjing Huang, Haishan Lu, Bin Pan, Hanyi Pan, Yanlun Song, Qianqian Deng, Xiaojun Jin, Guiling Shi

“…Additionally, functional enrichment of RNA‐sequencing of CNIH4‐knocked down CESC cell lines showed the association of CNIH4 to the PI3K‐Akt signaling pathway. …”
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Therapeutic Potential of a Small-Molecule STAT3 Inhibitor in a Mouse Model of Colitis by Prema Robinson, Kelsey Montoya, Emily Magness, Emma Rodriguez, Viviana Villalobos, Nikita Engineer, Peng Yang, Uddalak Bharadwaj, Thomas Kris Eckols, David John Tweardy

“…Methods: We examined mortality, weight loss, rectal bleeding, diarrhea, colon shortening, apoptosis of colonic CD4+ T-cells, and colon infiltration with IL-17-producing cells following 7-day administration of DSS (5%) to transgenic STAT3α knock-in (STAT3β-deficient; ΔβΔβ) mice and wild-type (WT) littermate cage control mice. …”
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Ethanol Extract of <i>Rosa rugosa</i> Ameliorates Acetaminophen-Induced Liver Injury via Upregulating Sirt1 and Subsequent Potentiation of LKB1/AMPK/Nrf2 Cascade in Hepatocytes by Yecheng Lei, Xiao Lei, Anqi Zhu, Shijie Xie, Tiantian Zhang, Chuo Wang, Anning Song, Xiaoming Wang, Guangwen Shu, Xukun Deng

“…As expression of LKB1 was knocked down, ERS still upregulated Sirt1 but failed to activate AMPK/Nrf2 cascade or suppress cytotoxicity provoked by APAP. …”
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Using <i>Staphylococcus aureus</i> Cas9 to Expand the Scope of Potential Gene Targets for Genome Editing in Soybean by Yan Zhang, Yupeng Cai, Shi Sun, Tianfu Han, Li Chen, Wensheng Hou

“…The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) is a revolutionary genome editing technology that has been used to achieve site-specific gene knock-out, large fragment deletion, or base editing in many plant species including soybean (<i>Glycine</i><i>max</i>). …”
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SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells by Yang Yang, Rongfang Qiu, Siyu Zhao, Lin Shen, Bufu Tang, Qiaoyou Weng, Ziwei Xu, Liyun Zheng, Weiqian Chen, Gaofeng Shu, Yajie Wang, Zhongwei Zhao, Minjiang Chen, Jiansong Ji

“…Transcriptomic analysis of the consequences of knocking down SMYD3, MTA1, or MTA2 in HCC cells showed that SMYD3/NuRD complex targets a cohort of genes, some of which are critically involved in cell growth and migration. qChIP analyses showed that SMYD3 knockdown led to a significant reduction in the binding of MTA1 or MTA2 to the promoters of IGFBP4 and led to a significant decrease in H4K20me3 and a marked increase in H4Ac at the IGFBP4 promoter. …”
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Sequence-Specific Features of Short Double-Strand, Blunt-End RNAs Have RIG-I- and Type 1 Interferon-Dependent or -Independent Anti-Viral Effects by Abhilash Kannan, Maarit Suomalainen, Romain Volle, Michael Bauer, Marco Amsler, Hung V. Trinh, Stefano Vavassori, Jana Pachlopnik Schmid, Guilherme Vilhena, Alberto Marín-González, Ruben Perez, Andrea Franceschini, Christian von Mering, Silvio Hemmi, Urs F. Greber

“…The antiviral effects of miR29b-1* were independent of a central AUAU-motif inducing dsRNA bending, as mimics with disrupted AUAU-motif were anti-viral in normal but not RIG-I knock-out (KO) or IFNAR2-KO cells. The screening of a library of scrambled short dsRNA sequences identified also anti-viral mimics functioning independently of RIG-I and IFNAR2, thus exemplifying the diverse anti-viral mechanisms of short blunt-end dsRNAs.…”
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Galantamine ameliorates experimental pancreatitis by Dane A. Thompson, Tea Tsaava, Arvind Rishi, Sam J. George, Tyler D. Hepler, Daniel Hide, Valentin A. Pavlov, Michael Brines, Sangeeta S. Chavan, Kevin J. Tracey

“…Experiments utilizing vagotomy, gene knock out (KO) technology and the use of nAChR antagonists were also performed. …”
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Overexpression of long noncoding RNA 4933425B07Rik leads to renal hypoplasia by inactivating Wnt/β-catenin signaling pathway by Shanshan Xue, Xuanjin Du, Minghui Yu, Haixin Ju, Lihong Tan, Yaxin Li, Jialu Liu, Chunyan Wang, Xiaohui Wu, Xiaohui Wu, Hong Xu, Qian Shen

“…On the other hand, these genes were more highly expressed when Rik was knocked out. These results imply that overabundance of Rik might inhibit the Wnt/β-catenin signaling pathway, which may result in renal hypoplasia. …”
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Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway by Tian Kaisai, Zheng Mantang, Yuan Tailei, Zheng Liying, Chen Xiaoping, Jin Mingming, Zhang Yi

“…Conclusion: In summary, we found that hsa_circ_0013561 is a pro-tumor circRNA in NPC, which can reduce the activation of JAK2/STAT3 pathway by knocking down hsa_circ_0013561, thereby slowing down the malignant progression of NPC. …”
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