Transcriptional response of endometrial cells to insulin, cultured using microfluidics by Soo Young Baik, Alisha Miani, Haidee Tinning, Dapeng Wang, Daman J Adlam, Peter T Ruane, Niamh Forde

“…Transfection of siRNA for RAPSN successfully knocked down expression (P < 0.05), but this did not have any effect on cellular morphology. …”
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Toosendanin targeting eEF2 impedes Topoisomerase I & II protein translation to suppress esophageal squamous cell carcinoma growth by Xuechao Jia, Penglei Wang, Chuntian Huang, Dengyun Zhao, Qiong wu, Bingbing Lu, Wenna Nie, Limeng Huang, Xueli Tian, Pan li, Kyle Vaughn Laster, Yanan Jiang, Xiang Li, Honglin Li, Zigang Dong, Kangdong Liu

“…Results We found that eEF2 were highly expressed in ESCC and negatively associated with the prognosis of ESCC patients. Knocking down of eEF2 suppressed the cell proliferation and colony formation of ESCC. eEF2 bond with the mRNA of Topoisomerase II (TOP1) and Topoisomerase II (TOP2) and enhanced the protein biosynthesis of TOP1 and TOP2. …”
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Genome-Wide Scans and Transcriptomic Analyses Characterize Selective Changes as a Result of Chlorantraniliprole Resistance in <i>Plutella xylostella</i> by Wenting Dai, Bin Zhu, Marcel van Tuinen, Tao Zhu, Dongliang Shang, Pedro Almeida, Pei Liang, Hidayat Ullah, Liping Ban

“…Further confirmation came from the weighted gene co-expression network analysis, which indicated that genes in the significant module associated with chlorantraniliprole resistance were enriched in pathways for exogenous detoxification, and that <i>CYP6B6-like</i> represented a hub gene in the “darkred” module. Furthermore, RNAi knock-down of <i>CYP6B6-like</i> increases <i>P. xylostella</i> sensitivity to chlorantraniliprole. …”
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Deep Learning-Based Identification of Intraocular Pressure-Associated Genes Influencing Trabecular Meshwork Cell Morphology by Connor J. Greatbatch, MBBS, Qinyi Lu, MD, PhD, Sandy Hung, PhD, Son N. Tran, PhD, Kristof Wing, MBBS, Helena Liang, MD, PhD, Xikun Han, PhD, Tiger Zhou, FRANZCO, PhD, Owen M. Siggs, MD, PhD, David A. Mackey, FRANZCO, MD, Guei-Sheung Liu, PhD, Anthony L. Cook, PhD, Joseph E. Powell, PhD, Jamie E. Craig, FRANZCO, DPhil, Stuart MacGregor, PhD, Alex W. Hewitt, FRANZCO, PhD

“…Methods: Sixty-two genes at 55 loci associated with IOP variation were knocked out in primary TMC lines. All cells underwent high-throughput microscopy imaging after being stained with a 5-channel fluorescent cell staining protocol. …”
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Disruption of DDX53 coding sequence has limited impact on iPSC-derived human NGN2 neurons by Muhammad Faheem, Eric Deneault, Roumiana Alexandrova, Deivid C. Rodrigues, Giovanna Pellecchia, Carole Shum, Mehdi Zarrei, Alina Piekna, Wei Wei, Jennifer L. Howe, Bhooma Thiruvahindrapuram, Sylvia Lamoureux, P. Joel Ross, Clarrisa A. Bradley, James Ellis, Stephen W. Scherer

“…Methods We used the clustered regularly interspaced short palindromic repeats (CRISPR) gene editing strategy to knock out DDX53 protein by inserting 3 termination codons (3TCs) into two different induced pluripotent stem cell (iPSC) lines. …”
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Characterization of a DCL2-Insensitive <i>Tomato Bushy Stunt Virus</i> Isolate Infecting <i>Arabidopsis thaliana</i> by Marco Incarbone, Hélene Scheer, Jean-Michel Hily, Lauriane Kuhn, Mathieu Erhardt, Patrice Dunoyer, Denise Altenbach, Christophe Ritzenthaler

“…A dsRNA-centered immunoprecipitation analysis allowed the identification of TBSV-associated host components including DRB2 and DRB4, which perfectly localized to replication sites, and NFD2 that accumulated in larger viral factories in which peroxisomes cluster. By challenging knock-out mutants for key RNAi factors, we showed that TBSV-BS3Ng undergoes a non-canonical RNAi defensive reaction. …”
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Functional analysis of Rickettsia monacensis strain humboldt folA dihydrofolate reductase gene via complementation assay by Brandon Hill, Ben Schafer, Nolan Vargas, Danny Zamora, Rohan Shrotri, Sarahi Perez, Geoffrey Farmer, Aren Avon, Anirudh Pai, Hirotada Mori, Jianmin Zhong

“…The mutant containing strain Humboldt folA subclone and a pFE604 clone of the knocked-out folA gene was cured of pFE604. Curing of the folA mutant E. coli construct was successful using acridine orange and 43.5 °C incubation temperature. …”
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A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the StomachSummary by Daisuke Douchi, Akihiro Yamamura, Junichi Matsuo, Jung-Won Lee, Napat Nuttonmanit, Yi Hui Melissa Lim, Kazuto Suda, Mitsuhiro Shimura, Sabirah Chen, ShuChin Pang, Kazuyoshi Kohu, Mari Kaneko, Hiroshi Kiyonari, Atsushi Kaneda, Hideyuki Yoshida, Ichiro Taniuchi, Motomi Osato, Henry Yang, Michiaki Unno, Jimmy Bok-Yan So, Khay Guan Yeoh, Linda Shyue Huey Chuang, Suk-Chul Bae, Yoshiaki Ito

“…Methods: To understand the oncogenic nature of this mutation in vivo, we generated the RUNX3R122C knock-in mice. Stomach tissues were harvested, followed by histologic and immunofluorescence staining, organoid culture, flow cytometry to isolate gastric corpus isthmus and nonisthmus epithelial cells, and RNA extraction for transcriptomic analysis. …”
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Modulating Golgi Stress Signaling Ameliorates Cell Morphological Phenotypes Induced by CHMP2B with Frontotemporal Dementia-Associated p.Asp148Tyr by Shoya Fukatsu, Maho Okawa, Miyu Okabe, Mizuka Cho, Mikinori Isogai, Takanori Yokoi, Remina Shirai, Hiroaki Oizumi, Masahiro Yamamoto, Katsuya Ohbuchi, Yuki Miyamoto, Junji Yamauchi

“…Finally, when transfecting Hsp47 or knocking down Arf4 with small interfering (si)RNA, cellular phenotypes in mutant-expressing cells were recovered. …”
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CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling P... by Hai-zhou Ji, Li Chen, Mi Ren, Sang Li, Tong-yu Liu, Hong-ju Chen, Hui-hui Yu, Yang Sun

“…However, these protective effects were largely canceled when <i>CXCR2</i> was knocked down. In summary, CXCL8 can activate the NF-κB signaling pathway in endothelial cells in a CXCR2-dependent manner. …”
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Opposite Effects of Moderate and Extreme Cx43 Deficiency in Conditional Cx43-Deficient Mice on Angiotensin II-Induced Cardiac Fibrosis by Laura Valls-Lacalle, Corall Negre-Pujol, Cristina Rodríguez, Saray Varona, Antoni Valera-Cañellas, Marta Consegal, Jose Martínez-González, Antonio Rodríguez-Sinovas

“…Here, we analyzed the role of Cx43 in myocardial fibrosis caused by angiotensin II (AngII) using Cx43^fl/fl and Cx43^Cre-ER(T)/fl inducible knock-out (Cx43 content: 50%) mice treated with vehicle or 4-hydroxytamoxifen (4-OHT) to induce a Cre-ER(T)-mediated global deletion of the Cx43 floxed allele. …”
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Acetone Prospect as an Additive to Allow the Use of Castor and Sunflower Oils as Drop-In Biofuels in Diesel/Acetone/Vegetable Oil Triple Blends for Application in Diesel Engines by Laura Aguado-Deblas, Jesus Hidalgo-Carrillo, Felipa M. Bautista, Diego Luna, Carlos Luna, Juan Calero, Alejandro Posadillo, Antonio A. Romero, Rafael Estevez

“…Despite the low calorific value of ACE limits its ratio in the mixtures due to engine knocking problems, the experimental results reveal an excellent performance for the blends containing up to 16-18% of ACE and 22-24% of SVO. …”
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IL-1 Mediates Chronic Stress-Induced Hyperalgesia Accompanied by Microglia and Astroglia Morphological Changes in Pain-Related Brain Regions in Mice by Barbara Fülöp, Ágnes Hunyady, Noémi Bencze, Viktória Kormos, Nikolett Szentes, Ádám Dénes, Nikolett Lénárt, Éva Borbély, Zsuzsanna Helyes

“…Female and male C57Bl/6J wild-type (WT) and IL-1αβ-deficient (knock-out: IL-1 KO) mice were exposed to 6 h of immobilization/day for 4 weeks. …”
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Histone Deacetylase 3 Governs β-Estradiol-ERα-Involved Endometrial Tumorigenesis via Inhibition of STING Transcription by Guofang Chen, Qiang Yan, Lin Liu, Xinyue Wen, Hongliang Zeng, Shasha Yin

“…We explored the possible relationship of acetylation alteration in STING regulation by ChIP analysis and Co-IP, and we knocked out <i>STING</i> in ECC1 and Ishikawa cells using CRISPR-Cas9 to further confirm the critical role of STING restoration induced by HDAC3 inhibitor RGFP-966 in the proliferation and apoptosis. …”
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<i>MnHR4</i> Functions during Molting of <i>Macrobrachium nipponense</i> by Regulating 20E Synthesis and Mediating 20E Signaling by Huwei Yuan, Wenyi Zhang, Hui Qiao, Shubo Jin, Sufei Jiang, Yiwei Xiong, Yongsheng Gong, Hongtuo Fu

“…Finally, the function of the <i>MnHR4</i> gene in the molting process of <i>M. nipponense</i> was studied by counting the molting frequency. After knocking down <i>MnHR4</i>, the molting frequency of <i>M. nipponense</i> decreased significantly. …”
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Expression of a Constitutively Active Form of <i>Hck</i> in Chondrocytes Activates Wnt and Hedgehog Signaling Pathways, and Induces Chondrocyte Proliferation in Mice by Viviane K. S. Kawata Matsuura, Carolina Andrea Yoshida, Hisato Komori, Chiharu Sakane, Kei Yamana, Qing Jiang, Toshihisa Komori

“…Furthermore, <i>Hck</i> knock-down reduced the proliferation of primary chondrocytes. …”
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Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence?... by Victoria Liedtke, Laura Rose, Rico Hiemann, Abdullah Nasser, Stefan Rödiger, Alena Bonaventura, Laura Winkler, Mandy Sowa, Michael Stöckle, Peter Schierack, Kerstin Junker, Dirk Roggenbuck

“…We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). …”
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Metformin Is a Pyridoxal-5′-phosphate (PLP)-Competitive Inhibitor of SHMT2 by Angela Tramonti, Elisabet Cuyàs, José Antonio Encinar, Matthias Pietzke, Alessio Paone, Sara Verdura, Aina Arbusà, Begoña Martin-Castillo, Giorgio Giardina, Jorge Joven, Alexei Vazquez, Roberto Contestabile, Francesca Cutruzzolà, Javier A. Menendez

“…Isotope tracing studies in SHMT1 knock-out cells confirmed that metformin decreased the SHMT2-channeled serine-to-formate flux and restricted the formate utilization in thymidylate synthesis upon overexpression of the metformin-unresponsive yeast equivalent of mitochondrial complex I (mCI). …”
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Analysis of asymptomatic Drosophila models for ALS and SMA reveals convergent impact on functional protein complexes linked to neuro-muscular degeneration by Marina L. Garcia-Vaquero, Marjorie Heim, Barbara Flix, Marcelo Pereira, Lucile Palin, Tânia M. Marques, Francisco R. Pinto, Javier de Las Rivas, Aaron Voigt, Florence Besse, Margarida Gama-Carvalho

“…Results Transcriptome analysis revealed that the three proteins do not bind to the same RNA molecules and that only a limited set of functionally unrelated transcripts is commonly affected by their knock-down. However, through our integrative approach we were able to identify a concerted effect on protein functional modules, albeit acting through distinct targets. …”
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<i>RTA1</i> Is Involved in Resistance to 7-Aminocholesterol and Secretion of Fungal Proteins in <i>Cryptococcus neoformans</i> by Emily S. Smith-Peavler, Ronakkumar Patel, Adejumoke Mary Onumajuru, Bethany G. Bowring, Joyce L. Miller, Jean Michel Brunel, Julianne T. Djordjevic, Moses M. Prabu, Erin E. McClelland

“…To determine the role of <i>RTA1</i> in <i>Cn</i>, the knock-out (<i>rta1Δ</i>) and reconstituted (<i>rta1Δ+RTA1</i>) strains were created and phenotypically tested. …”
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