Stearoyl-CoA Desaturases1 Accelerates Non-Small Cell Lung Cancer Metastasis by Promoting Aromatase Expression to Improve Estrogen Synthesis by Jiaping Chen, Yangwei Wang, Wangyang Meng, Rong Zhao, Wei Lin, Han Xiao, Yongde Liao

“…Western blotting assays demonstrated that CYP19A1, along with β-catenin protein levels, was reduced in SCD1 knocked-down cells, and estrogen concentration was reduced (<i>p</i> < 0.05) in cell culture medium measured by enzyme-linked immunosorbent assay. …”
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BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction by Yan-nan Xu, Wen Xu, Xu Zhang, Dan-yang Wang, Xin-rui Zheng, Wei Liu, Jia-mei Chen, Gao-feng Chen, Cheng-hai Liu, Ping Liu, Yong-ping Mu

“…In vitro, the Numb gene was overexpressed or knocked down in the WB-F344 cell line by lentivirus transfection, Then, cells were subjected immunofluorescence staining and the detection of mRNA levels of related factors, which provided further evidence supporting the results from in vivo experiments. …”
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Functional Characterisation of the Autophagy ATG12~5/16 Complex in <i>Dictyostelium discoideum</i> by Malte Karow, Sarah Fischer, Susanne Meßling, Roman Konertz, Jana Riehl, Qiuhong Xiong, Ramesh Rijal, Prerana Wagle, Christoph S. Clemen, Ludwig Eichinger

“…<i>Dictyostelium discoideum</i> <i>atg5</i> single, <i>atg5/12</i> double, and <i>atg5/12/16</i> triple gene knock-out mutant strains displayed similar defects in the conjugation of ATG8 to phosphatidylethanolamine, development, and cell viability upon nitrogen starvation. …”
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L-Type Ca_v1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells by Julien Louradour, Olivier Bortolotti, Eleonora Torre, Isabelle Bidaud, Ned Lamb, Anne Fernandez, Jean-Yves Le Guennec, Matteo E. Mangoni, Pietro Mesirca

“…Methods: To investigate the role of L-type Ca_v1.3 Ca²⁺ channels in the adrenergic regulation of automaticity in dormant SANC, we used a knock-in mouse strain in which the sensitivity of L-type Ca_v1.2 α1 subunits to dihydropyridines (DHPs) was inactivated (<i>Ca_v1.2^DHP−/−</i>), enabling the selective pharmacological inhibition of Ca_v1.3 by DHPs. …”
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NF-κB Signaling Modulates miR-452-5p and miR-335-5p Expression to Functionally Decrease Epithelial Ovarian Cancer Progression in Tumor-Initiating Cells by Rahul D. Kamdar, Brittney S. Harrington, Emma Attar, Soumya Korrapati, Jyoti Shetty, Yongmei Zhao, Bao Tran, Nathan Wong, Carrie D. House, Christina M. Annunziata

“…We validated the miR-seq findings via qPCR in TIC or adherent conditions with <i>RELA</i> or <i>RELB</i> knocked-down. We confirmed decreased expression of hsa-miR-452-5p when either <i>RELA</i> or <i>RELB</i> were depleted and increased expression of hsa-miR-335-5p when <i>RELA</i> was depleted. …”
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Integrin α2 and β1 Cross-Communication with mTOR/AKT and the CDK-Cyclin Axis in Hepatocellular Carcinoma Cells by Mazen A. Juratli, He Zhou, Elsie Oppermann, Wolf O. Bechstein, Andreas Pascher, Felix K.-H. Chun, Eva Juengel, Jochen Rutz, Roman A. Blaheta

“…Blockade of these integrins led to cell cycle arrest in G2/M and diminished the number of tumor cell clones. Knocking down the integrins α2 or β1 suppressed ILK, reduced FAK-phosphorylation and diminished AKT/mTOR, as well as the proteins of the CDK-Cyclin axis. …”
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The Interactive Complex between Cytomegalovirus Kinase vCDK/pUL97 and Host Factors CDK7–Cyclin H Determines Individual Patterns of Transcription in Infected Cells by Martin Schütz, Arne Cordsmeier, Christina Wangen, Anselm H. C. Horn, Emanuel Wyler, Armin Ensser, Heinrich Sticht, Manfred Marschall

“…Major hallmarks of this interplay are the interaction between cyclin H and vCDK/pUL97, which is consistently detectable across various conditions and host cell types of infection, the decrease or increase in pUL97 kinase activity resulting from cyclin H knock-down or elevated levels, respectively, and significant trans-stimulation of human CDK7 activity by pUL97 in vitro. …”
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High expression of SLC27A2 predicts unfavorable prognosis and promotes inhibitory immune infiltration in acute lymphoblastic leukemia by Lihua Lu, Jiazheng Li, Yongzhi Zheng, Luting Luo, Yan Huang, Jianda Hu, Yanxin Chen

“…Furthermore, we analyzed the relationship between SLC27A2 gene expression and immune cell infiltration using the ESTIMATE method, which was evaluated using the TIGER platform. Finally, we knocked down SLC27A2 in the Jurkat ALL cell line and conducted cell proliferation, western blotting, flow cytometry, and CCK-8 assays to elucidate the biological function of SLC27A2 in ALL. …”
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Oncogenic zinc finger protein ZNF687 accelerates lung adenocarcinoma cell proliferation and tumor progression by activating the PI3K/AKT signaling pathway by Mingchun Li, Zhihua Liu, Zan Hou, Xiangcai Wang, Huaqiu Shi, Yamei Li, Xuewen Xiao, Zhixian Tang, Jianqiong Yang, Yaoling Luo, Minhong Zhang, Ming Chen

“…ZNF687 overexpression enhanced LUAD growth, migration, invasion and colony formation, and the cell cycle G1‐S transition; additionally, it promoted the epithelial‐mesenchymal transition (EMT). In contrast, knocking down ZNF687 showed to have the opposite impact. …”
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Anti-Inflammatory Effects of Synthetic Peptides Based on Glucocorticoid-Induced Leucine Zipper (GILZ) Protein for the Treatment of Inflammatory Bowel Diseases (IBDs) by Musetta Paglialunga, Sara Flamini, Raffaele Contini, Marta Febo, Erika Ricci, Simona Ronchetti, Oxana Bereshchenko, Graziella Migliorati, Carlo Riccardi, Stefano Bruscoli

“…Subsequently, PEP-1 was further evaluated in two in vivo experimental colitis models (chemically induced by DNBS administration and spontaneous colitis induced in IL-10 knock-out (KO) mice (to assess its effectiveness in counteracting inflammation. …”
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The conserved Mediator subunit cyclin C (CCNC) is required for brown adipocyte development and lipid accumulation by Ziyi Song, Alus M. Xiaoli, Youlei Li, Gerile Siqin, Tian Wu, Randy Strich, Jeffrey E. Pessin, Fajun Yang

“…Methods: We generated conditional knock-out mice by crossing Ccncflox/flox mice with Myf5Cre, Ucp1Cre or AdipoqCre transgenic mice to investigate the role of CCNC in BAT development and function. …”
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Loss of selenoprotein W in murine macrophages alters the hierarchy of selenoprotein expression, redox tone, and mitochondrial functions during inflammation by Sougat Misra, Tai-Jung Lee, Aswathy Sebastian, John McGuigan, Chang Liao, Imhoi Koo, Andrew D. Patterson, Randall M. Rossi, Molly A. Hall, Istvan Albert, K. Sandeep Prabhu

“…In this study, we utilized Selenow knock-out (KO) murine bone marrow-derived macrophages (BMDMs) to address the role of SELENOW in inflammation following stimulation with bacterial endotoxin lipopolysaccharide (LPS). …”
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IL-1B drives opposing responses in primary tumours and bone metastases; harnessing combination therapies to improve outcome in breast cancer by Claudia Tulotta, Diane V. Lefley, Charlotte K. Moore, Ana E. Amariutei, Amy R. Spicer-Hadlington, Lewis A. Quayle, Russell O. Hughes, Khawla Ahmed, Victoria Cookson, Catherine A. Evans, Jayakumar Vadakekolathu, Paul Heath, Sheila Francis, Emmanuel Pinteaux, A. Graham Pockley, Penelope D. Ottewell

“…Using syngeneic IL-1B/IL1R1 knock out mouse models in combination with genetic manipulation of tumour cells to overexpress IL-1B/IL1R1, we found that IL-1B signalling elicited an opposite response in primary tumours compared with bone metastases. …”
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Multiomics characterization and verification of clear cell renal cell carcinoma molecular subtypes to guide precise chemotherapy and immunotherapy by Jialin Meng, Aimin Jiang, Xiaofan Lu, Di Gu, Qintao Ge, Suwen Bai, Yundong Zhou, Jun Zhou, Zongyao Hao, Fangrong Yan, Linhui Wang, Haitao Wang, Juan Du, Chaozhao Liang

“…We also confirm that SETD2 is a tumor suppressor in ccRCC, along with the decreased SETD2 protein level in advanced tumor stage, and knock‐down of SETD2 leads to the promotion of cell proliferation, migration, and invasion. …”
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Chronic Activation of LXRα Sensitizes Mice to Hepatocellular Carcinoma by Yang Xie, Runzi Sun, Li Gao, Jibin Guan, Jingyuan Wang, Aaron Bell, Junjie Zhu, Min Zhang, Meishu Xu, Peipei Lu, Xinran Cai, Songrong Ren, Pengfei Xu, Satdarshan P. Monga, Xiaochao Ma, Da Yang, Yulan Liu, Binfeng Lu, Wen Xie

“…In the current study, we found that systemic activation of LXRα in the VP‐LXRα knock‐in (LXRαKI) mice or hepatocyte‐specific activation of LXRα in the VP‐LXRα transgenic mice sensitized mice to liver tumorigenesis induced by the combined treatment of diethylnitrosamine (DEN) and 3,3',5,5'‐tetrachloro‐1,4‐bis (pyridyloxy) benzene (TCPOBOP). …”
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Targeting Oncogenic Mutant p53 and BCL-2 for Small Cell Lung Cancer Treatment by Victoria Neely, Alekhya Manchikalapudi, Khanh Nguyen, Krista Dalton, Bin Hu, Jennifer E. Koblinski, Anthony C. Faber, Sumitra Deb, Hisashi Harada

“…A recent study with oncogenic mutant (Onc)-p53 knock-in mouse models of SCLC suggests gain-of-function activity can attenuate chemotherapeutic efficacy. …”
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miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway by Jing ZHAO, Ya-mei WU, Yao ZHANG, Shu-yue TANG, Shun-shun HAN, Can CUI, Bo TAN, Jie YU, Hou-yang KANG, Guang-deng CHEN, Meng-gen MA, Qing ZHU, Hua-dong YIN

“…Additionally, our investigation of IRS2 in chicken hepatocytes revealed that knocking down IRS2 has the same effects as overexpressing miR-27b-5p. …”
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ERRγ-inducible FGF23 promotes alcoholic liver injury through enhancing CYP2E1 mediated hepatic oxidative stress by Yoon Seok Jung, Kamalakannan Radhakrishnan, Seddik Hammad, Sebastian Müller, Johannes Müller, Jung-Ran Noh, Jina kim, In-Kyu Lee, Sung Jin Cho, Don-Kyu Kim, Yong-Hoon Kim, Chul-Ho Lee, Steven Dooley, Hueng-Sik Choi

“…The role of FGF23 was examined in hepatocyte-specific knock-out (LKO) mice of cannabinoid receptor type 1 (CB1R), estrogen related receptor γ (ERRγ), or FGF23. …”
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Overexpression of <i>GmRIQ2-like</i> (<i>Glyma.04G174400</i>) Enhances the Tolerance of Strong Light Stress and Reduces Photoinhibition in Soybean (<i>Glycine max</i> (L.) Merr.) by Jing Deng, Dongmei Li, Huayi Yin, Li Ma, Jiukun Zhang, Binbin Zhang

“…The quantitative real-time (qRT)-PCR results revealed that <i>GmRIQ2-like-</i>overexpression (OE) and -knock-out (KO) transgenic soybean seedlings were cultivated successfully. …”
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Super-enhancer driven SOX2 promotes tumor formation by chromatin re-organization in nasopharyngeal carcinomaResearch in context by Shang-Xin Liu, Chong Wang, Ruo-Bin Lin, Wei-Yue Ding, Gaurab Roy, Hong-Bo Wang, Ting Yang, Qian Liu, Yi-Ling Luo, Shui-Lin Jin, Mu-Sheng Zeng, Bo Zhao, Qian Zhong

“…In addition, SOX2 was highly expressed in NPC and is correlated with short survival in patients with NPC. Knock-down of SOX2 suppressed tumor growth in vitro and in vivo. …”
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