Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis by Yu Zou, Lu Qiu, Aowen Xie, Wenyuan Han, Shangbo Zhang, Jinshan Li, Shumiao Zhao, Yingjun Li, Yunxiang Liang, Yongmei Hu

“…As a proof of concept, two extracellular protease genes epr and bpr were continuously knocked out using this system, and it only took 2.5 days to complete one round of genome editing. …”
Get full text

<i>asb5a</i>/<i>asb5b</i> Double Knockout Affects Zebrafish Cardiac Contractile Function by Wanwan Cai, Yuequn Wang, Ying Luo, Luoqing Gao, Jian Zhang, Zhigang Jiang, Xiongwei Fan, Fang Li, Yulian Xie, Xiushan Wu, Yongqing Li, Wuzhou Yuan

“…Morpholino interference was used to target <i>asb5b</i> in wild-type and <i>asb5a</i>-knockout zebrafish. Knocking down <i>asb5b</i> in the wild-type had no phenotypic impact, but simultaneous <i>asb5b</i> knockdown in <i>asb5a</i>-knockout homozygotes led to severe pericardial cavity enlargement and atrial dilation. …”
Get full text

Identification of the principal neuropeptide MIP and its action pathway in larval settlement of the echiuran worm Urechis unicinctus by Zhi Yang, Long Zhang, Wenqing Zhang, Xinhua Tian, Wenyuan Lai, Dawei Lin, Yuxin Feng, Wenwen Jiang, Zhengrui Zhang, Zhifeng Zhang

“…Whole-mount in situ hybridization identified two selected ciliary genes, Tctex1d2 and Cfap45, were specially expressed in circumoral ciliary cells of the early-segmentation larvae. Knocking down Tctex1d2 mRNA levels by in vivo RNA interference significantly increased the larval settlement rate. …”
Get full text

Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development by Hui Sheng, Yiwen Guo, Linlin Zhang, Junxing Zhang, Manning Miao, Haoyun Tan, Debao Hu, Xin Li, Xiangbin Ding, Guangpeng Li, Hong Guo

“…At the same time, Co-Immunoprecipitation (CO-IP) assay showed that MSTN gene interacted with extracellular matrix related protein type I collagen α 1 (COL1A1), and knocking down the expression of COL1A1 could inhibit the activity of adhesion, PI3K-AKT and ribosome pathway, thus inhibit BSMSCs proliferation. …”
Get full text

Intracellular microRNA expression patterns influence cell death fates for both necrosis and apoptosis by Akira Sato, Akihiro Yamamoto, Akira Shimotsuma, Yoko Ogino, Naoki Funayama, Yui Takahashi, Akiko Hiramoto, Yusuke Wataya, Hye‐Sook Kim

“…In this study, we investigated the intracellular miRNA expression profiles of FUdR‐treated F28‐7 cells (necrotic condition), GA plus FUdR‐treated F28‐7 cells (apoptotic condition), and FUdR‐treated F28‐7‐A cells (apoptotic condition) through miRNA microarray analysis. In addition, we knocked down Dicer, a key molecule for the expression of mature miRNAs, in F28‐7 cells to examine whether it modulates FUdR‐induced cell death. …”
Get full text

PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage responseResearch in context by Wei Yue, Xinyu Li, Xiaolu Zhan, Lei Wang, Jihong Ma, Meiyu Bi, Qilong Wang, Xiaoyang Gu, Bingteng Xie, Tong Liu, Hongyan Guo, Xin Zhu, Chen Song, Jie Qiao, Mo Li

“…Methods: A CRISPR-based knock-in technology was used to establish stable BRCA1 mutant cancer cells. …”
Get full text

Altered Mitochondrial Function and Accelerated Aging Phenotype in Neural Stem Cells Derived from Dnm1l Knockout Embryonic Stem Cells by Seung-Bin Na, Bong-Jong Seo, Tae-Kyung Hong, Seung-Yeon Oh, Yean-Ju Hong, Jae-Hoon Song, Sang-Jun Uhm, Kwonho Hong, Jeong-Tae Do

“…In this study, we investigated the effect of knocking out the <i>dynamin 1-like protein</i> (<i>Dnm1l</i>) gene, a key regulator of mitochondrial fission, in neural stem cells (NSCs) differentiated from <i>Dnm1l</i> knockout embryonic stem cells (<i>Dnm1l</i>^−/− ESCs). …”
Get full text

The Epithelial–Mesenchymal Transcription Factor <i>SNAI1</i> Represses Transcription of the Tumor Suppressor miRNA <i>let-7</i> in Cancer by Hanmin Wang, Evgeny Chirshev, Nozomi Hojo, Tise Suzuki, Antonella Bertucci, Michael Pierce, Christopher Perry, Ruining Wang, Jeffrey Zink, Carlotta A. Glackin, Yevgeniya J. Ioffe, Juli J. Unternaehrer

“…In vivo, nanoparticle-delivered siRNA successfully knocked down <i>SNAI1</i> in orthotopic patient-derived xenografts, accompanied by reduced stemness and increased <i>let-7</i> expression, and reduced tumor burden. …”
Get full text

ATF3-Induced Mammary Tumors Exhibit Molecular Features of Human Basal-Like Breast Cancer by Leqin Yan, Sally Gaddis, Luis Della Coletta, John Repass, Katherine Leslie Powell, Melissa S. Simper, Yueping Chen, Michelle Byrom, Yi Zhong, Kevin Lin, Bin Liu, Yue Lu, Jianjun Shen, Michael C. MacLeod

“…Finally, we show through knock-down experiments that <i>ATF3</i> may directly modulate <i>MIR145/143</i> expression. …”
Get full text

Transplantation Amid a Pandemic: The Fall and Rise of Kidney Transplantation in the United States by Shivani S. Bisen, BA, Laura B. Zeiser, ScM, Brian Boyarsky, MD, PhD, William Werbel, MD, Jon Snyder, PhD, Jacqueline Garonzik-Wang, MD, PhD, Macey L. Levan, JD, PhD, Dorry L. Segev, MD, PhD, Allan B. Massie, PhD, MHS

“…The recent Omicron surge knocked LDKT from 25% below baseline (incidence rate ratio [IRR] = 0.690.750.81) during the Delta wave to 38% below baseline (IRR = 0.580.620.67). …”
Get full text

MicroRNA Let-7e in the Mouse Prefrontal Cortex Differentiates Restraint-Stress-Resilient Genotypes from Susceptible Genotype by Joanna Solich, Magdalena Kolasa, Agata Faron-Górecka, Jacek Hajto, Marcin Piechota, Marta Dziedzicka-Wasylewska

“…Three strains of mice with various susceptibilities to restraint stress (RS), i.e., mice with a knocked out norepinephrine transporter gene (NET-KO), SWR/J and C57BL/6J (WT) mice were shown to serve as a good model to study the molecular mechanisms underlying different stress-coping strategies. …”
Get full text

Sapap3 Deletion Anomalously Activates Short-Term Endocannabinoid-Mediated Synaptic Plasticity by Chen, Meng, Wan, Yehong, Ade, Kristen, Ting, Jonathan, Feng, Guoping, Calakos, Nicole

“…SAP90/PSD-95-associated proteins (SAPAPs) are a family of postsynaptic proteins unique to excitatory synapses. Using Sapap3 knock-out (KO) mice, we find that, in the absence of SAPAP3, striatal medium spiny neuron (MSN) excitatory synapses exhibit eCB-mediated synaptic depression under conditions that do not normally activate this process. …”
Get full text
Get full text

Exogenous delivery of chaperonin subunit fragment ApiCCT1 modulates mutant Huntingtin cellular phenotypes by Sontag, Emily M., Joachimiak, Lukasz A., Tan, Zhiqun, Tomlinson, Anthony, Glabe, Charles G., Potkin, Steven G., Frydman, Judith, Thompson, Leslie M., Housman, David E

“…ApiCCT1r reduces mHtt-mediated toxicity in immortalized striatal cells derived from full-length knock-in HD mice, suggesting that therapeutic benefit may extend beyond effects on aggregation. …”
Get full text
Get full text

Enhancing tumor cell response to chemotherapy through nanoparticle-mediated codelivery of siRNA and cisplatin prodrug by Xu, Xiaoyang, Xie, Kun, Zhang, Xue-Qing, Pridgen, Eric M., Park, Ga Young, Cui, Danica S., Shi, Jinjun, Kantoff, Philip W., Lippard, Stephen J., Walker, Graham C., Farokhzad, Omid C., Langer, Robert S, Wu, Jun

“…We demonstrated the potency of the siRNA-containing NPs to knock down target genes efficiently both in vitro and in vivo. …”
Get full text
Get full text
Get full text
Get full text
Get full text

Mechanically induced integrin ligation mediates intracellular calcium signaling with single pulsating cavitation bubbles by Li, Fenfang, Park, Tae Hyun, Sankin, George, Gilchrist, Christopher, Liao, Defei, Chan, Chon U., Mao, Zheng, Hoffman, Brenton D., Zhong, Pei

“…Methods: Here, laser-induced cavitation microbubble was used to stimulate individual HEK293T cells either genetically knocked out or expressing Piezo1 ion channels with different normalized bubble-cell distance. …”
Get full text

The Knockout of the <i>ASIP</i> Gene Altered the Lipid Composition in Bovine Mammary Epithelial Cells via the Expression of Genes in the Lipid Metabolism Pathway by Tao Xie, Yinuo Liu, Huixian Lu, Ambreen Iqbal, Mengru Ruan, Ping Jiang, Haibin Yu, Jilun Meng, Zhihui Zhao

“…The <i>ASIP</i> gene was knocked out in bMECs by using CRISPR/Cas9 technology. …”
Get full text

EEF1A1 is Involved the Regulating Neuroinflammatory Processes in Parkinson's Disease by Zaolaguli Aisha, Jing Lei, Yan Zhang, Jianhua Ma

“…Our study’s primary objective was to knock down EEF1A1 in U251 cells and preliminarily explore the role of EEF1A1 in PD neuroinflammation. …”
Get full text

Identification of a Novel Yellow Fever Virus Cellular Restriction Factor by Jinhua Xiang, James H. McLinden, Qing Chang, Thomas M. Kaufman, Jennifer L. Welch, Nirjal Bhattarai, Jack T. Stapleton

“…YFV (17D strain) and Zika (PR strain) were used to infect the different cell lines, and viral replication was measured by viral RNA and infectivity. Attempts to knock out PTPRE by CRISPR were unsuccessful, suggesting that PTPRE may be required for cell survival. …”
Get full text

Role of CrRLK1L Cell Wall Sensors HERCULES1 and 2, THESEUS1, and FERONIA in Growth Adaptation Triggered by Heavy Metals and Trace Elements by Julia Richter, Marie Ploderer, Gaëlle Mongelard, Laurent Gutierrez, Marie-Theres Hauser

“…In contrast hypocotyl elongation of the knock-out mutant, fer-4, was hypersensitive to Ni but exhibited a higher tolerance to Cd, Cu, Pb, and Zn. …”
Get full text

Mendelian Randomization Study With Clinical Follow‐Up Links Metabolites to Risk and Severity of Pulmonary Arterial Hypertension by Elham Alhathli, Thomas Julian, Zain Ul Abideen Girach, A. A. Roger Thompson, Christopher Rhodes, Stefan Gräf, Niamh Errington, Martin R. Wilkins, Allan Lawrie, Dennis Wang, Johnathan Cooper‐Knock

Get full text