Silent information regulator 3 modulates lysosome function to inhibit apoptosis of U87 cell by mTOR pathway by ZHANG Xudong, ZHANG Xudong, SHAN Litianyi, WANG Rong

“…Finally, the change of lysosome membrane protein after overexpression of TFEB and Sirt3 was detected in U87 cells with knocking down and out of Sirt3. Results Sirt3 was significantly highly expressed in glioma tissue, and the level was closely associated with poor survival of the glioma patients. …”
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High expression of centromere protein N as novel biomarkers for gastric adenocarcinoma by Xiaojie Wang, Keyuan Zhang, Cun Fu, Fei Wu, Junjie Zhang, Bin Han, Hai Pan, Lan Luan

“…When CENP‐N expression in AGS cells was knocked down, cell proliferation dramatically reduced (p < .05) and the percentage of cells in the S and G2‐M phases decreased significantly (p < .05). …”
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CUX1—Transcriptional Master Regulator of Tumor Progression in Pancreatic Neuroendocrine Tumors by Sebastian Krug, Julia Weissbach, Annika Blank, Aurel Perren, Johannes Haybaeck, Volker Fendrich, Anja Rinke, Thomas Mathias Gress, Jonas Rosendahl, Patrick Michl

“…Validation of differentially regulated genes was performed in Bon-1 and QGP1 cells with knock-down and overexpression strategies. CUX1 expression assessed by a predefined immunoreactivity score (IRS) was significantly associated with shorter progression-free survival (PFS) of pre-treated PanNET patients (23 vs. 8 months; <i>p</i> = 0.005). …”
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Polymerase II–Associated Factor 1 Complex-Regulated FLOWERING LOCUS C-Clade Genes Repress Flowering in Response to Chilling by Zeeshan Nasim, Hendry Susila, Suhyun Jin, Geummin Youn, Ji Hoon Ahn

“…Consistent with the reduced expression of FLC and the FLC-clade members FLM/MAF1 and MAF2–MAF5, chromatin immunoprecipitation (ChIP)-quantitative PCR assays showed reduced levels of the permissive epigenetic modification H3K4me3/H3K36me3 and increased levels of the repressive modification H3K27me3 at their chromatin. Knocking down MAF2–MAF5 using artificial microRNAs (amiRNAs) in the flc flm background (35S::amiR-MAF2–5 flc flm) resulted in significantly earlier flowering than flc flm mutants and even earlier than short vegetative phase (svp) mutants at 10°C. …”
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Development Procedure for Performance Estimation and Main Dimensions Calculation of a Highly-Boosted Ethanol Engine with Water Injection by Thiago Rodrigo Vieira da Silva, Nilton Antonio Diniz Netto, Jeanine Costa Santos, Augusto Cesar Teixeira Malaquias, José Guilherme Coelho Baêta

“…Furthermore, a port water injection system was fitted to the engine in order to assist the combustion process by mitigating the knock tendency. The predicted overall engine performance was obtained by carrying out a GT-Power^TM 1D-CFD simulation, whose results pointed to a maximum torque of 279 Nm from 2000 to 4000 rpm and an indicated peak power of 135 kW at 5500 rpm. …”
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Cardiac Mitochondria Dysfunction in Dyslipidemic Mice by Alicja Braczko, Barbara Kutryb-Zajac, Agata Jedrzejewska, Oliwia Krol, Paulina Mierzejewska, Magdalena Zabielska-Kaczorowska, Ewa M. Slominska, Ryszard T. Smolenski

“…The analyses were performed in 3- and 6-month-old knock-out mice for low-density lipoprotein receptor (<i>Ldlr−/−</i>) and compared to wild-type C57Bl/6J mice (WT). …”
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The Effect of Prohibitins on Mitochondrial Function during <i>Octopus tankahkeei</i> Spermiogenesis by Jingqian Wang, Xinming Gao, Chen Du, Daojun Tang, Congcong Hou, Junquan Zhu

“…To further investigate the effect of <i>Ot</i>-PHBs on mitochondrial function, we knocked down <i>Ot</i>-<i>phb1</i> and observed a decrease in mtDNA content, along with increases in ROS levels and the expressions of mitochondria-induced apoptosis-related genes <i>bax</i>, <i>bcl2</i>, and <i>caspase-3</i> mRNA. …”
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Reduced folate carrier 1 is present in retinal microvessels and crucial for the inner blood retinal barrier integrity by Gokce Gurler, Nevin Belder, Mustafa Caglar Beker, Melike Sever-Bahcekapili, Gokhan Uruk, Ertugrul Kilic, Muge Yemisci

“…Results Our analyses on whole mount retinas and trypsin digested microvessel samples of adult mice revealed the presence of RFC1 in the inner BRB and colocalization with endothelial cells and pericytes. Knocking down RFC1 expression via siRNA delivery resulted in the disintegration of tight junction proteins and collagen-4 in twenty-four hours, which was accompanied by significant endogenous IgG extravasation. …”
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Plasma Membrane-Associated Proteins Identified in Arabidopsis Wild Type, <i>lbr2-2</i> and <i>bak1-4</i> Mutants Treated with LPSs from <i>Pseudomonas</i> <i>syringae</i> and <i>Xa... by Benedict C. Offor, Msizi I. Mhlongo, Ian A. Dubery, Lizelle A. Piater

“…In this study, Arabidopsis wild type (WT) and T-DNA knock out mutants (<i>lbr2-2</i> and <i>bak1-4</i>) were treated with LPS chemotypes from <i>Pseudomonas syringae</i> pv. …”
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Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells by Junaid Ali Shah, Yujuan Miao, Jinmeng Chu, Wenqi Chen, Qingzhi Zhao, Chengyu Cai, Saadullah Khattak, Fei Wang, Jingji Jin

“…Herein, we showed evidence that the INO80 complex transcriptionally controls microRNA-372 (miR-372) expression through RNA-Seq analysis and a series of biological experiments. Knocking down multiple subunits in the INO80 complex, including the <i>INO80</i> catalytic subunit, <i>YY1</i>, <i>Ies2</i>, and <i>Arp8</i>, can significantly increase the expression level of miR-372. …”
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Intronic miR-744 Inhibits Glioblastoma Migration by Functionally Antagonizing Its Host Gene MAP2K4 by Max Hübner, Christian Ludwig Hinske, David Effinger, Tingting Wu, Niklas Thon, Friedrich-Wilhelm Kreth, Simone Kreth

“…Re-expression of miR-744 reduced levels of TGFB1, DVL2, and the host MAP2K4, and mitigated activity of TGFB1 and DVL2 downstream targets SMAD2/3 and beta-Catenin. TGFB1 knock-down repressed MAP2K4 expression. Conclusion: MiR-744 acts as an intrinsic brake on its host. …”
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A Novel Wall-Associated Kinase TaWAK-5D600 Positively Participates in Defense against Sharp Eyespot and <i>Fusarium</i> Crown Rot in Wheat by Haijun Qi, Xiuliang Zhu, Wenbiao Shen, Zengyan Zhang

“…By analyzing the RNA-sequencing data of wheat inoculated with <i>R. cerealis</i> and <i>F. pseudograminearum</i>, we found that transcript abundance of <i>TaWAK-5D600</i> (<i>TraesCS5D02G268600</i>) on chromosome 5D was significantly upregulated, and that its upregulated transcript levels in response to both pathogens were higher compared with other <i>TaWAK</i> genes. Importantly, knock-down of <i>TaWAK-5D600</i> transcript impaired wheat resistance against the fungal pathogens <i>R. cerealis</i> and <i>F. pseudograminearum</i>, and significantly repressed expression of defense-related genes in wheat, <i>TaSERK1</i>, <i>TaMPK3</i>, <i>TaPR1</i>, <i>TaChitinase3</i>, and <i>TaChitinase4</i>. …”
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Integrated High-Throughput Sequencing, Microarray Hybridization and Degradome Analysis Uncovers MicroRNA-Mediated Resistance Responses of Maize to Pathogen <i>Curvularia lunata</i> by Weiwei Wang, Zhen Liu, Xinyuan An, Yazhong Jin, Jumei Hou, Tong Liu

“…To further confirm the role of PC-732 in the interaction of maize and <i>C. lunata</i>, the miRNA was silenced through STTM (short tandem target mimic) technology, and we found that knocking down PC-732 decreased the susceptibility of maize to <i>C. lunata</i>. …”
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Identification of Novel Insulin Resistance Related ceRNA Network in T2DM and Its Potential Editing by CRISPR/Cas9 by Marwa Matboli, Marwa Mostafa Kamel, Nada Essawy, Meram Mohamed Bekhit, Basant Abdulrahman, Ghada F. Mohamed, Sanaa Eissa

“…CRISPR/Cas9 successfully knocked out <i>LncRNA-RP11-773H22.4</i>, as evidenced by the reversal of the gene expression of the identified network at RNA and protein levels to the normal expression pattern after gene editing. …”
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Mechanism of CPNE3 regulating proliferation, migration and invasion of non-small cell lung cancer cells by CAI Xin, LIU Zeyi, HUANG Jian'an

“…Overexpression of CPNE3 promoted cell proliferation in vivo (P < 0.001), and upregulated phosphorylation of AKT, ERK, and EGFR (P < 0.05, P < 0.05, P < 0.01). Conclusion Knocking CPNE3 down can inhibit the proliferation, migration and invasion of A549 cells, while its overexpression can promote the proliferation ability of A549 cells in vivo, which may be related to EGFR signaling pathway and downstream AKT/ERK signaling pathway. …”
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Aprepitant attenuates NLRC4-dependent neuronal pyroptosis via NK1R/PKCδ pathway in a mouse model of intracerebral hemorrhage by Peng Jin, Dongqing Qi, Yuhui Cui, Cameron Lenahan, John H. Zhang, Xiaogen Tao, Shuixiang Deng, Jiping Tang

“…Activation of NK1R or PKCδ abolished these neuroprotective effects of Aprepitant after ICH. Similarly, knocking down NLRC4 using siRNA produced similar neuroprotective effects. …”
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Long noncoding RNASEH1‐AS1 exacerbates the progression of non‐small cell lung cancer by acting as a ceRNA to regulate microRNA‐516a‐5p/FOXK1 and thereby activating the Wnt/β‐cateni... by Chan Zhang, Jian Huang, Ke Lou, Hui Ouyang

“…Knockdown of RNASEH1‐AS1 expression inhibited NSCLC cell growth, metastatic capacities, and epithelial‐mesenchymal transition and promoted the apoptosis in vitro, whereas RNASEH1‐AS1 overexpression exerted the opposite effects. Additionally, knocking down RNASEH1‐AS1 expression suppressed tumor growth in vivo. …”
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METTL3 Regulates Osteoclast Biological Behaviors via iNOS/NO-Mediated Mitochondrial Dysfunction in Inflammatory Conditions by Di Li, Jinlin He, Caihong Fang, Yiwen Zhang, Mingli He, Zhanqi Zhang, Jinsong Hou, Qiong Xu

“…In this study, we observed that the total m⁶A content and METTL3 expression decreased during LPS-induced osteoclastogenesis. After knocking down METTL3, we found reduced levels of the number of osteoclasts, osteoclast-related gene expression and bone resorption area. …”
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Age-Dependent Sex Differences in Perineuronal Nets in an <i>APP</i> Mouse Model of Alzheimer’s Disease Are Brain Region-Specific by Rayane Rahmani, Naiomi Rambarack, Jaijeet Singh, Andrew Constanti, Afia B. Ali

“…This was consistent with the results of WGCNA performed on normalised data on microglial cells isolated from age-matched, late-stage male and female wild-type and APP knock-in mice, which revealed one microglial module that showed differential expression associated with tissue, age, genotype, and sex, which showed enrichment for fc-receptor-mediated phagocytosis. …”
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LncRNA SATB2-AS1 overexpression represses the development of hepatocellular carcinoma through regulating the miR-3678-3p/GRIM-19 axis by Jiang Huang, Yunfang Yang, Fulan Zhao, Zhuo Zhang, Jian Deng, Wei Lu, Xian Jiang

“…Mechanistically, SATB2-AS1 inactivated STAT3/HIF-1α and strengthened GRIM-19 expression. After knocking down GRIM-19 with small interfering RNA (siRNA), the malignant phenotypes of HCC cells were enhanced. …”
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