Novel Therapeutic Approaches for KRAS-Mutated Lung Cancer Involving LZTR1 Genetic Alteration by Raj N. Sewduth, Tonci Ivanisevic, Peihua Zhao, Anna A. Sablina

“…While <i>LZTR1</i> depletion in LUAD cell lines did not affect proliferation in the cell culture, the knock-out (KO) of <i>Lztr1</i> in a mouse model with <i>Kras G12D</i> oncogenic mutations caused a clear and significant acceleration of tumor progression in the Lztr1 loss groups, indicating that Lztr1 can affect tumor onset and progression. …”
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A modular plasmid toolkit applied in marine bacteria reveals functional insights during bacteria-stimulated metamorphosis by Amanda T. Alker, Morgan V. Farrell, Alpher E. Aspiras, Tiffany L. Dunbar, Andriy Fedoriouk, Jeffrey E. Jones, Sama R. Mikhail, Gabriella Y. Salcedo, Bradley S. Moore, Nicholas J. Shikuma

“…Using these tools, we quantified constitutive and native promoter expression, developed reporter strains that enable the imaging of host-bacteria interactions, and used CRISPR interference (CRISPRi) to knock down a secondary metabolite and a host-associated gene. …”
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Caspase-3 Promotes Diabetic Kidney Disease Through Gasdermin E-Mediated Progression to Secondary Necrosis During Apoptosis by Wen S, Wang ZH, Zhang CX, Yang Y, Fan QL

“…Here we showed that caspase-3 inhibition prevented GSDME activation and cell death in glucose-treated tubular cells. Specifically, knocking down GSDME directly inhibited secondary necrosis and fibrogenesis.Conclusion: These data suggest GSDME-dependent secondary necrosis plays a crucial role in renal injury, and provides a new insight into the pathogenesis of DKD and a promising target for its treatment.Keywords: GSDME, DFNA5, secondary necrosis, diabetic kidney disease, Z-DEVD-FMK…”
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SOCS3 Regulates Dectin-2-Induced Inflammation in PBMCs of Diabetic Patients by Mohammed J. A. Haider, Zahraa Albaqsumi, Fahd Al-Mulla, Rasheed Ahmad, Fatema Al-Rashed

“…CRP, IL-1β, and TNF-α concentrations were determined by ELISA. siRNA transfection and Western blotting were performed to assess p-Syk and p-NF-kB expression. siRNA transfection was performed to knock down the gene of interest. Our results show that Dectin-2 expression was the highest in monocytes compared with other leukocyte subpopulations. …”
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Aldehyde Dehydrogenase Mutation Exacerbated High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease with Gut Microbiota Remodeling in Male Mice by Sien-Sing Yang, Yi-Hsun Chen, Jui-Ting Hu, Ching-Feng Chiu, Shao-Wen Hung, Yi-Chih Chang, Chien-Chao Chiu, Hsiao-Li Chuang

“…Male (M) and female (F) wild-type (WT) and ALDH2 knock-in C57BL/6J mice (4–5 weeks old) were fed a high-fat diet for 16 weeks. …”
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The role of Nrf1 and Nrf2 in the regulation of glutathione and redox dynamics in the developing zebrafish embryo by Karilyn E. Sant, Jason M. Hansen, Larissa M. Williams, Nancy L. Tran, Jared V. Goldstone, John J. Stegeman, Mark E. Hahn, Alicia Timme-Laragy

“…To determine whether the doubling of glutathione or the dynamic changes in glutathione redox potential are mediated by zebrafish paralogs of Nrf transcription factors, morpholino oligonucleotides were used to knock down translation of Nrf1 and Nrf2 (nrf1a, nrf1b, nrf2a, nrf2b). …”
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MALT-1 shortens lifespan by inhibiting autophagy in the intestine of C. elegans by Julie Vérièpe-Salerno, Silvia Podavini, Marcus J.C. Long, Irina Kolotuev, Muriel Cuendet, Margot Thome

“…These findings suggest that MALT-1 limits the lifespan of C. elegans by acting as an inhibitor of an early step of autophagy in the intestine.Abbreviations: AP-1: activator protein-1; AL: autolysosome; AP: autophagosome; ATG: Autophagy-related gene, BCL10: B cell lymphoma-10; CARD: caspase-recruitment domain; CARMA: CARD-MAGUK, C. elegans: Caenorhabditis elegans; CBM complex: CARMA-BCL10-MALT1 complex; EGFR: epidermal growth factor receptor; ER: endoplasmic reticulum; EV: empty vector; GFP: green fluorescent protein; GPCRs: G-protein coupled receptors; Ig: Immunoglobulin; Int: intestinal; ITAMs: immunoreceptor tyrosine-mediated activation motifs; KO: knock-out; LC3: microtubule-associated protein 1A/1B-light chain 3; LUBAC: linear ubiquitin chain assembly complex; MALT-1: mucosa-associated lymphoid tissue protein-1; NF-κB: nuclear factor-kappa B; NFKI: NF-κB inhibitor; NGM: nematode growth medium; ns: not significant; NS: nervous system; OE: overexpressed; RER: rough endoplasmic reticulum; RNAi: RNA interference; TCR: T cell receptor; TEM: Transmission electron microscopy; TRAF6: TNF receptor-associated factor-6.…”
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UCP1 alleviates renal interstitial fibrosis progression through oxidative stress pathway mediated by SIRT3 protein stability by Wei Xiong, Zhiyong Xiong, Anni Song, Chuntao Lei, Chen Ye, Hua Su, Chun Zhang

“…Simultaneously, UCP1 reduced the ROS production by increasing the stability of SIRT3. When SIRT3 was knocked down, the production of ROS decreased. Conclusions Elevating the expression of UCP1 can inhibit the occurrence of oxidative stress by stabilizing SIRT3, thereby reducing EMT and ECM accumulation, and ultimately alleviating renal interstitial fibrosis. …”
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Engineered M2a macrophages for the treatment of osteoarthritis by Chi Liang, Song Wu, Guang Xia, Junjie Huang, Junjie Huang, Zi Wen, Wenxiu Zhang, Xu Cao, Xu Cao

“…We constructed a stable macrophage that “locked” in anti-inflammatory and pro-regenerative M2a polarity (L-M2a) by simultaneously knocking out tumor necrosis factor receptor 1 (TNFR1) and overexpressing IL-4 using Cas9-ribonuclear proteins (Cas9-RNP) and electroporation. …”
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Circular RNA METTL9 contributes to neuroinflammation following traumatic brain injury by complexing with astrocytic SND1 by Chunling Huang, Lulu Sun, Chenyang Xiao, Wenjun You, Li Sun, Siye Wang, Zhijun Zhang, Su Liu

“…To examine potential involvement of circMETTL9 in neurodegeneration and loss of function following TBI, circMETTL9 expression in cortex was knocked-down by microinjection of a shcircMETTL9 adeno-associated virus. …”
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<i>Toxoplasma gondii</i> <i>ADSL</i> Knockout Provides Excellent Immune Protection against a Variety of Strains by Luyao Wang, Ding Tang, Chenghang Yang, Jing Yang, Rui Fang

“…In addition, ideal vaccine production is a challenge in the future. In this study, we knocked out the adenylosuccinate lyase (<i>ADSL</i>) gene and found that the gene reduces the growth rate of <i>T. gondii</i> tachyzoites in vitro under standard growth conditions by plaque or replication experiments. …”
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Shrimp miR-965 induced the human melanoma stem-like cell apoptosis and inhibited their stemness by disrupting the MCL-1-ER stress-XBP1 feedback loop in a cross-species manner by Wenlin Wu, Chenxi Xu, Xiaobo Zhang, An Yu, Le Shu

“…Results In the present study, our results showed that myeloid cell leukemia sequence 1 (MCL-1) knocking down induced ER stress and apoptosis, and the expression reduction of stemness associated genes in MSLC, which implied a significant role of MCL-1 in MSLC. …”
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siRNAs to Knockdown Antiviral Chemokine-related Genes in FRhK-4 Cells by Eunyoung Park, Yoonjeong Yoo, Sangeun Park, Changsun Choi, Yohan Yoon

“…These results indicate that si-F-IRF3-1 and si-F-IRF3-3, and si-F-IRF7 successfully knocked down IRF3 and IRF7 in FRhK-4 cells, respectively and suppressed antiviral chemokines. …”
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Heat shock protein 90 is involved in the regulation of HMGA2-driven growth and epithelial-to-mesenchymal transition of colorectal cancer cells by Chun-Yu Kao, Pei-Ming Yang, Ming-Heng Wu, Chi-Chen Huang, Yi-Chao Lee, Kuen-Haur Lee

“…The use of the second-generation Hsp90 inhibitor, NVP-AUY922, considerably knocked down HMGA2 expression, and the effects of Hsp90 and HMGA2 knockdown were similar. …”
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LRRC59 promotes proliferation, invasion and inhibition of apoptosis in bladder urothelial carcinoma cells by ZHAGN Peng, LIANG Peihe

“…After the expression of LRRC59 in T24 and 5637 cells were knocked down by sh-RNA techniques (shRNA-1, shRNA-2 and shRNA-3 for knockdown, and shRNA-NC as blank control). …”
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Eugenol Inhibits Ox-LDL-Induced Proliferation and Migration of Human Vascular Smooth Muscle Cells by Inhibiting the Ang II/MFG-E8/MCP-1 Signaling Cascade by He JH, Li XJ, Wang SP, Guo X, Chu HX, Xu HC, Wang YS

“…To investigate the mechanisms, Ang II receptors were inhibited by 1000 nM valsartan, MFG-E8 was knocked down by shRNA, MCP-1 was inhibited by siRNA, and MFG-E8 was overexpressed using plasmids.Results: The findings from this study elucidated the stimulatory impact of ox-LDL on the proliferation and functionality of HVSMCs. …”
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24780 Investigating the role of mycobacterial lipid antigens and CD1-restricted T cells in host-protective tuberculosis immunity using a guinea pig model by Macallister Harris, James Dilisio, Hadley Gary, Edward Chan, D. Branch Moody, Brendan Podell

“…It is anticipated that knocking out CD1 expression will lead to impaired immunity, and increase severity of disease as noted by pathologic lesions/bacterial burden, and systemic spread; in contrast, CD1 enhancement will limit the severity of tuberculosis. …”
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Interferon‐regulatory factor‐1 boosts bevacizumab cardiotoxicity by the vascular endothelial growth factor A/14‐3‐3γ axis by Xuan‐Ying Chen, Meng‐Qi Xie, Wei‐Lin Huang, Wen‐Juan Li, Yan‐Ni Lv, Xiao‐Ping Peng

“…The release of lactate dehydrogenase (LDH), cardiac troponins T (cTnT), and creatine kinase MB (CK‐MB) was measured using the enzyme linked immunosorbent assay. The effects of knocking down IRF1 on BVZ‐induced mice were analysed in vivo. …”
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The effects of restraint stress on ceramide metabolism disorders in the rat liver: the role of CerS6 in hepatocyte injury by Yichang Liu, Zhaoling Sun, Qiuli Sun, Li Wang, Chuan Wang, Yingmin Li, Chunling Ma, Weibo Shi, Guozhong Zhang, Yiming Dong, Xiaojing Zhang, Bin Cong

“…Furthermore, CORT induced sequential phosphorylation of AMPK and p38 MAPK proteins, and inhibition of the p38 MAPK pathway using SB203580 mitigated the CORT-induced elevation in CerS6 protein. Knocking down CerS6 in hepatocytes inhibited both the increase in C16:0 ceramide and the release of mitochondrial cytochrome c induced by CORT. …”
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RNA Interference-Mediated Knockdown of Tryptophan 2,3-Dioxygenase and Kynurenine-3-Monooxygenase in Monochamus Alternatus: Implications for Insect Control by Minghui Zhang, Xiaoqian Weng, Qing Li, Liangjing Sheng, Yajie Guo, Liya Xiong, Feiping Zhang, Songqing Wu

“…<i>MaTDO</i> and <i>MaKMO</i> were knocked down using RNA interference technology in different periods of expression, and the temporal expression changes were obtained using RT-qPCR technology. …”
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