Differential effect on myelination through abolition of activity dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in... by Korrell, K, Disser, J, Parley, K, Vadisiute, A, Requena-Komura, M, Fodder, H, Pollart, C, Knott, G, Molnar, Z, Hoerder-Suabedissen, A

“…We compared two models of ‘silencing' layer V and/or VI cortical projection neurons from early stages by either decreasing their excitability through Kir2.1 expression, an inward rectifying potassium channel, introduced through in utero electroporation at embryonic day (E)13.5, or inhibiting regulated vesicular release through Cre‐dependent knock‐out of synaptosomal associated protein 25 kDA (SNAP25). …”

Epistatic rescue of Nkx2.5 adult cardiac conduction disease phenotypes by prospero-related homeobox protein 1 and HDAC3. by Risebro, C, Petchey, L, Smart, N, Gomes, J, Clark, J, Vieira, J, Yanni, J, Dobrzynski, H, Davidson, S, Zuberi, Z, Tinker, A, Shui, B, Tallini, Y, Kotlikoff, M, Miquerol, L, Schwartz, R, Riley, P

“…Anatomic studies, on a Cx40(EGFP) background, revealed Cre-mediated knock-down of Prox1 restored the anatomy of the atrioventricular node and His-Purkinje network both of which were severely hypoplastic in Nkx2.5(Cre/+) littermates. …”

The old and the new: mouse models for evaluating the therapeutic margin of radiation in lung cancer by Lourenco, L

“…Fractionation schedules of tRTX led to pneumonitis and pulmonary fibrosis. Knock-out of myeloperoxidase activity was associated with decreased early and late normal tissue responses to tRTX. …”

Chromatin regulation of lifespan in C. elegans by Chocian, K

“…A secondary RNAi screen involving full lifespan monitoring confirmed knock-downs of four genes, <em>isw-1</em>, <em>cbp-1</em>, <em>mes-2</em> and <em>jmjd-3.2</em>, results in extension of lifespan. …”

Proteomics in viral disease by Gangadharan, B

“…Vav ubiquitination was restored and viral replication was diminished when β-Pix was knocked down providing a new candidate target for inhibiting HIV replication. …”

Liver specific microRNA control of adenovirus serotype five by Cawood, R

“…RT-QPCR for E1A mRNA demonstrated a 29-fold reduction when compared to Ad5 WT in murine liver whilst western blot confirmed that all E1A protein variants were knocked down. Viral genomic replication was also reduced in mouse liver by 25-fold compared to Ad5 WT. …”

Separating plasticity-induced closure and residual stress contributions to fatigue crack retardation following an overload by Salvati, E, Zhang, H, Soon Fong, K, Song, X, Korsunsky, A

“…Afterwards, the SIF range reduction ratio, β, which represents the “knock down” factor with respect to the steady state growth was assessed. …”

Development of palm methyl ester microemulsions as aerosol insecticides by Othman, Rohana

“…The bioefficacy test on both products showed good performance in knocking down insects by 100%, 24 hours of mortality rate as compared to the Malaysian Standard Aerosol. …”
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Indoor and outdoor residual spraying of a novel formulation of deltamethrin K-Othrine® (Polyzone) for the control of simian malaria in Sabah, Malaysia by Ahmed, Md Atique, Rohani, A., Fakhriy, H. Ahmad, Suzilah, I., Zurainee, M. N., W. M. A., Wan Nadjah, Ariffin, M. Mohd, Shakirudin, N. Mohamad, M. S., Mohd Afiq, Jenarun, J., Tanrang, Y., Lee, H. L.

“…Deltamethrin K-Othrine® (PolyZone) at 25 mg/m2 was the most effective with regards to ability to high mortality and effective knock down (KD). The vector population was reduced significantly post-spraying and reduction in breeding sites as well. …”
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Cotton (<i>Gossypium hirsutum</i>) VIRMA as an N6-Methyladenosine RNA Methylation Regulator Participates in Controlling Chloroplast-Dependent and Independent Leaf Development by Xiaoyu Huang, Nigara Abuduwaili, Xinting Wang, Miao Tao, Xiaoqian Wang, Gengqing Huang

“…<i>GhVIR-A</i> and <i>GhVIR-D</i> were ubiquitously expressed in different tissues and relatively higher expressed in leaves and main stem apexes (MSA). Knocking down the expression of <i>GhVIR</i> genes by the virus-induced gene silencing (VIGS) system influences leaf cell size, cell shape, and total cell numbers, thereby determining cotton leaf morphogenesis. …”
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The archaeal KEOPS complex possesses a functional Gon7 homolog and has an essential function independent of the cellular t6A modification level by Pengju Wu, Qi Gan, Xuemei Zhang, Yunfeng Yang, Yuanxi Xiao, Qunxin She, Jinfeng Ni, Qihong Huang, Yulong Shen

“…Strikingly, none of the genes encoding aKEOPS subunits, including Pcc1 and Pcc1‐like, can be deleted in the wild type and in a t6A modification complementary strain named TsaKI, implying that the aKEOPS complex is essential for an additional cellular process in this archaeon. Knock‐down of the Cgi121 subunit leads to severe growth retardance in the wild type that is partially rescued in TsaKI. …”
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Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities by Yang Wang, Guobin Yin, Huanjiao Weng, Luyao Zhang, Guocheng Du, Jian Chen, Zhen Kang

“…As examples, the E. coli cell morphology was easily modified and new target genes of ergothioneine biosynthesis were quickly identified with ssl-sRNAs. ssl-sRNA and its designer sslRNAD enable researchers to rapidly design sRNAs for knocking down target genes.…”
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Strategies for Efficient Expression of Heterologous Monosaccharide Transporters in <i>Saccharomyces cerevisiae</i> by Marilia M. Knychala, Angela A. dos Santos, Leonardo G. Kretzer, Fernanda Gelsleichter, Maria José Leandro, César Fonseca, Boris U. Stambuk

“…In another approach, two arrestins known to be involved in the ubiquitinylation and endocytosis of sugar transporters (<i>ROD1</i> and <i>ROG3</i>) were knocked out, but only the <i>rog3</i> mutant allowed a significant improvement of growth and fermentation in glucose when either of the XUT permeases were expressed. …”
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Upregulation of NOXA by 10-Hydroxycamptothecin plays a key role in inducing fibroblasts apoptosis and reducing epidural fibrosis by Jihang Dai, Yu Sun, Lianqi Yan, Jingcheng Wang, Xiaolei Li, Jun He

“…The results from the TUNEL assay also showed a decreased rate of apoptosis in NOXA-knocked down fibroblasts. For the in vivo studies, we performed a laminectomy at the L1-L2 levels in rats and applied HCPT of different concentrations (0.2, 0.1, 0.05 mg/ml and saline) locally; the macroscopic histological assessment, hydroxyproline content analysis and histological staining were performed to evaluate the effect of HCPT on reducing epidural fibrosis. …”
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Unraveling the impact of sialic acids on the immune landscape and immunotherapy efficacy in pancreatic cancer by Manfred Wuhrer, Louis Boon, Di Wang, Yvette van Kooyk, Ernesto Rodriguez, Joke M M Den Haan, Nadine van Montfoort, Sandra J van Vliet, Kelly Boelaars, Laura Goossens-Kruijssen, Charlotte M de Winde, Dimitri Lindijer, Babet Springer, Irene van der Haar Àvila, Aram de Haas, Laetitia Wehry, Reina E Mebius

“…A sialic acid deficient KPC3 line was generated by genetic knock-out of the CMAS (cytidine monophosphate N-acetylneuraminic acid synthetase) enzyme, a critical enzyme in the synthesis of sialic acid-containing glycans. …”
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BMI1 fine-tunes gene repression and activation to safeguard undifferentiated spermatogonia fate by Ruiqi Liu, Yonglin Peng, Wenfei Du, Yunqiang Wu, Wen Zhang, Congxia Hu, Min Liu, Xinhua Liu, Ji Wu, Jielin Sun, Xiaodong Zhao

“…However, the mechanistic underpinnings of how BMI1 regulates spermatogonia fate remain elusive.Methods: We knocked down BMI1 by siRNA to investigate the role of BMI1 in undifferentiated spermatogonia. …”
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Multicopy Chromosome Integration and Deletion of Negative Global Regulators Significantly Increased the Heterologous Production of Aborycin in <i>Streptomyces coelicolor</i> by Jia-Yi Li, Jun-Yu Liang, Zhao-Yuan Liu, Yue-Zhao Yi, Jing Zhao, Zhi-Yong Huang, Jun Chen

“…Then, five regulatory genes, <i>phoU</i> (<i>SCO4228</i>), <i>wblA</i> (<i>SCO3579</i>), <i>SCO1712</i>, <i>orrA</i> (<i>SCO3008</i>) and <i>gntR</i> (<i>SCO1678</i>), which reportedly have negative effects on secondary metabolism, were further knocked out from the M1346::3<i>gul</i> genome by CRISPR/Cas9 technology. …”
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Differential Competitive Growth of Transgenic Subclones of Neuroblastoma Cells Expressing Different Levels of Cathepsin D Co-Cultured in 2D and 3D in Response to EGF: Implications... by Eleonora Secomandi, Andrea Esposito, Giulia Camurani, Chiara Vidoni, Amreen Salwa, Chiara Lualdi, Letizia Vallino, Alessandra Ferraresi, Ciro Isidoro

“…We compared the growth kinetics of such mixed clones in 2D and 3D models in response to EGF, and we found that the Over CD clone had an advantage for growth in suspension, while the CD knocked-down clone was favored for the adherent growth in 2D. …”
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T cell specific deletion of Casitas B lineage lymphoma-b reduces atherosclerosis, but increases plaque T cell infiltration and systemic T cell activation by Winnie G. Vos, Winnie G. Vos, Winnie G. Vos, Bram W. van Os, Bram W. van Os, Bram W. van Os, Myrthe den Toom, Linda Beckers, Cindy P.A.A. van Roomen, Claudia M. van Tiel, Bhopal C. Mohapatra, Hamid Band, Katrin Nitz, Katrin Nitz, Katrin Nitz, Christian Weber, Christian Weber, Christian Weber, Christian Weber, Dorothee Atzler, Dorothee Atzler, Dorothee Atzler, Menno P.J. de Winther, Menno P.J. de Winther, Menno P.J. de Winther, Laura A. Bosmans, Laura A. Bosmans, Laura A. Bosmans, Esther Lutgens, Esther Lutgens, Esther Lutgens, Tom T.P. Seijkens, Tom T.P. Seijkens, Tom T.P. Seijkens

“…We previously demonstrated that complete knock-out of CBL-B aggravated atherosclerosis in Apoe-/- mice, which was attributed to increased macrophage recruitment and increased CD8+ T cell activation in the plaque.MethodsTo further study the T cell specific role of CBL-B in atherosclerosis, Apoe-/- CD4creCblbfl/fl (Cbl-bcKO) mice and Apoe-/-CD4WTCblbfl/fl littermates (Cbl-bfl/fl) were fed a high cholesterol diet for ten weeks.ResultsCbl-bcKO mice had smaller atherosclerotic lesions in the aortic arch and root compared to Cbl-bfl/fl, and a substantial increase in CD3+ T cells in the plaque. …”
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Histidine-Rich Glycoprotein Suppresses the S100A8/A9-Mediated Organotropic Metastasis of Melanoma Cells by Nahoko Tomonobu, Rie Kinoshita, Hidenori Wake, Yusuke Inoue, I Made Winarsa Ruma, Ken Suzawa, Yuma Gohara, Ni Luh Gede Yoni Komalasari, Fan Jiang, Hitoshi Murata, Ken-ichi Yamamoto, I Wayan Sumardika, Youyi Chen, Junichiro Futami, Akira Yamauchi, Futoshi Kuribayashi, Eisaku Kondo, Shinichi Toyooka, Masahiro Nishibori, Masakiyo Sakaguchi

“…HRG also inhibited the S100A8/A9-induced migration and invasion of A375 melanoma cells. When we knocked down HRG in mice bearing skin melanoma, metastasis to both the brain and lungs was significantly enhanced. …”
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