Mouse Ataxin-2 Expansion Downregulates CamKII and Other Calcium Signaling Factors, Impairing Granule—Purkinje Neuron Synaptic Strength by Aleksandar Arsović, Melanie Vanessa Halbach, Júlia Canet-Pons, Dilhan Esen-Sehir, Claudia Döring, Florian Freudenberg, Nicoletta Czechowska, Kay Seidel, Stephan L. Baader, Suzana Gispert, Nesli-Ece Sen, Georg Auburger

“…Our <i>Atxn2</i>-CAG100-Knock-In mouse faithfully models features observed in patients at pre-onset, early and terminal stages. …”
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Enhancement of PD-L1-attenuated CAR-T cell function through breast cancer-associated fibroblasts-derived IL-6 signaling via STAT3/AKT pathways by Nisa Chuangchot, Pranisa Jamjuntra, Supaporn Yangngam, Piriya Luangwattananun, Suyanee Thongchot, Mutita Junking, Peti Thuwajit, Pa-Thai Yenchitsomanus, Chanitra Thuwajit

“…CAF-CM were treated in MDA-MB-231 and HCC70 TNBC cell lines and siIL-6 receptor (IL-6R) knocked down (KD) cells to determine the effect of CAF-derived IL-6 on Dox resistance by flow cytometry and on increased PD-L1 through STAT3, AKT and ERK1/2 pathways by Western blot analysis. …”
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An uncharacterized protein NY1 targets EAT1 to regulate anther tapetum development in polyploid rice by Nabieu Kamara, Zijun Lu, Yamin Jiao, Lianjun Zhu, Jinwen Wu, Zhixiong Chen, Lan Wang, Xiangdong Liu, Muhammad Qasim Shahid

“…Our previous research showed that the mutant (ny1) of LOC_Os07g32406 (NY1), which was generated by CRISPR/Cas9 knock-out in neo-tetraploid rice, showed low pollen fertility, low seed set, and defective chromosome behavior during meiosis. …”
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Molecular Mechanism of Mouse Uterine Smooth Muscle Regulation on Embryo Implantation by Rui Cao, Zhen-Shan Yang, Sui-Li Hu, Shi-Jin Liang, Shu-Miao Zhang, Song-Qi Zhu, Lin Lu, Cheng-Hong Long, Si-Tong Yao, Yong-Jiang Ma, Xiao-Huan Liang

“…Furthermore, progesterone inhibits the expression of Tagln through the progesterone receptor. Using siRNA to knock down Tagln in day 3 SMCs, we found that phalloidin staining is decreased, which confirms the critical role of Tagln in F-actin polymerization. …”
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CRISPR interference to evaluate modifiers of C9ORF72-mediated toxicity in FTD by Sarah Pickles, Sarah Pickles, Desiree Zanetti Alepuz, Yuka Koike, Mei Yue, Jimei Tong, Pinghu Liu, Yugui Zhou, Karen Jansen-West, Lillian M. Daughrity, Yuping Song, Michael DeTure, Björn Oskarsson, Neill R. Graff-Radford, Bradley F. Boeve, Ronald C. Petersen, Keith A. Josephs, Dennis W. Dickson, Dennis W. Dickson, Dennis W. Dickson, Michael E. Ward, Lijin Dong, Mercedes Prudencio, Mercedes Prudencio, Casey N. Cook, Casey N. Cook, Leonard Petrucelli, Leonard Petrucelli

“…Here, we provide proof-of-concept that we can simultaneously knock down Stmn2 and express the expanded repeat in the Chromosome 9 open reading frame 72 (C9ORF72) gene, successfully replicating features of C9-associated pathology. …”
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Near-infrared fluorescence imaging of hepatocellular carcinoma cells regulated by β-catenin signaling pathway by Jian Song, Tingting Ren, Yanheng Duan, Haitao Guo, Gang Wang, Yu Gan, Mengcai Bai, Xiaotian Dong, Zheng Zhao, Jiaze An

“…TCGA dataset analysis and immunohistochemistry were conducted to investigate the expression of OATPs or ABCGs in hepatocellular carcinoma (HCC) tissues. OATPs or ABCGs were knocked down and overexpressed in HCC cells using transient transfection by siRNA and plasmids or stable transfection by lentivirus. …”
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Extracellular superoxide dismutase (EC-SOD) R213G variant reduces mitochondrial ROS and preserves mitochondrial function in bleomycin-induced lung injury by Hanan Elajaili, Laura Hernandez-Lagunas, Peter Harris, Genevieve C. Sparagna, Raleigh Jonscher, Denis Ohlstrom, Carmen C. Sucharov, Russell P. Bowler, Hagir Suliman, Kristofer S. Fritz, James R. Roede, Eva S. Nozik

“…Mice (R213G mice) expressing a knock-in of this EC-SOD SNP exhibit elevated plasma and reduced lung EC-SOD content and activity and are protected against bleomycin-induced lung injury and inflammation. …”
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Identification of the shared gene signatures between pulmonary fibrosis and pulmonary hypertension using bioinformatics analysis by Hui Zhao, Hui Zhao, Lan Wang, Yi Yan, Yi Yan, Qin-Hua Zhao, Jing He, Rong Jiang, Ci-Jun Luo, Hong-Ling Qiu, Yu-Qing Miao, Su-Gang Gong, Ping Yuan, Wen-Hui Wu

“…Combining the results of crossing characteristic genes in ImmPort database and RT-PCR, the important gene IGF1 was obtained. Knocking down IGF1 could significantly reduce the proliferation and apoptosis resistance in pulmonary microvascular endothelial cells, pulmonary smooth muscle cells, and fibroblasts induced by hypoxia, platelet-derived growth factor-BB (PDGF-BB), and transforming growth factor-β1 (TGF-β1), respectively. …”
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HOXD-AS1 is a novel lncRNA encoded in HOXD cluster and a marker of neuroblastoma progression revealed via integrative analysis of noncoding transcriptome by Sampath, Prabha, Kuznetsov, Vladimir A., Kurochkin, Igor V., Yarmishyn, Aliaksandr A., Batagov, Arsen O., Tan, Jovina Z., Sundaram, Gopinath M.

“…Studying retinoid acid (RA) response of SH-SY5Y cell line, a model of human metastatic neuroblastoma, we found that HOXD-AS1 is a subject to morphogenic regulation, is activated by PI3K/Akt pathway and itself is involved in control of RA-induced cell differentiation. Knock-down experiments revealed that HOXD-AS1 controls expression levels of clinically significant protein-coding genes involved in angiogenesis and inflammation, the hallmarks of metastatic cancer. …”
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Silencing essential gene expression in Mycobacterium abscessus during infection by Yves-Marie Boudehen, Yara Tasrini, John Jairo Aguilera-Correa, Matthéo Alcaraz, Laurent Kremer

“…Herein, we generated a Tet-OFF-based system allowing to knock down the expression of mmpL3, encoding the mycolic acid transporter in mycobacteria. …”
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Molecular Characterization of Extensively Drug Resistant Salmonella Enterica Serovar Typhi Clinical Isolates from Lahore, Pakistan by Jabeen K, Saleem S, Jahan S, Nizamudin S, Arshad F, Huma ZE, Raza SM, Mehmood M, Roman M, Haq FU

“…Third generation cephalosporin were the empiric choice to treat typhoid fever in Pakistan, but acquisition of ESBLs have knocked them out of the arsenal. The current empiric choice is azithromycin which is vulnerable to resistance too. …”
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Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis by Shu Yang, Xuanping Zhou, Zhen Jia, Mali Zhang, Minghao Yuan, Yizhao Zhou, Jing Wang, Duo Xia

“…ADAMTS12 expression was reduced and the IL-1β-induced inflammatory chondrocyte injury was attenuated by silencing METTL3. Additionally, knocking down METTL3 in ACL-T-produced OA rats reduced ADAMTS12 expression in their cartilage tissues, thereby alleviating cartilage damage. …”
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A Rapid Method for Detecting Normal or Modified Plant and Algal Carbonic Anhydrase Activity Using <i>Saccharomyces cerevisiae</i> by Ashwani K. Rai, Robert J. DiMario, Remmy W. Kasili, Michael Groszmann, Asaph B. Cousins, David Donze, James V. Moroney

“…In this study, we generated a <i>Saccharomyces cerevisiae</i> CA knock-out (<i>ΔNCE103</i> or <i>ΔCA)</i> that has a high-CO₂-dependent phenotype (5% (<i>v</i>/<i>v</i>) CO₂ in air). …”
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Properties of the Caudal Pontine Reticular Nucleus Neurons Determine the Acoustic Startle Response in Cntnap2 KO Rats by Alice Zheng, Rajkamalpreet S. Mann, Dami Solaja, Brian L. Allman, Susanne Schmid

“…Similar to many autistic individuals, Cntnap2 knock-out rats (Cntnap2-⁣/-) are hyperreactive to sound as measured through the acoustic startle response. …”
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A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section Flavi Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus... by Perng-Kuang Chang

“…This developed system is highly efficient and allows knocking out multiple genes in A. flavus efficiently either by sequential transformation or by cotransformation of individual sgRNA vectors if desired. …”
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METTL3/IGF2BP2 axis affects the progression of colorectal cancer by regulating m6A modification of STAG3 by Jianmei Yi, Feng Peng, Jingli Zhao, Xiaosong Gong

“…Additionally, the expression of METTL3 was found to be upregulated in CRC tissues. Knocking down METTL3 resulted in a decrease in both the m6A level and protein expression of STAG3, inhibited cell proliferation and migration while promoting apoptosis, which were restored through STAG3 overexpression. …”
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Identifying CD1c as a potential biomarker by the comprehensive exploration of tumor mutational burden and immune infiltration in diffuse large B cell lymphoma by Xiaoyu Xiang, Li-Min Gao, Yuehua Zhang, Qiqi Zhu, Sha Zhao, Weiping Liu, Yunxia Ye, Yuan Tang, Wenyan Zhang

“…CREB1, AHR, and TOX were used to comprehensively explore the regulation of CD1c-related transcription factors and signaling pathways by the KnockTF database. We searched the GETx database to compare the mRNA expression levels of CD1c between DLBCL and normal tissues, and the results suggested a significant difference between them. …”
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Fusion Protein Vaccine Based on Ag85B and STEAP1 Induces a Protective Immune Response against Prostate Cancer by Linpei Guo, Hui Xie, Zheng Zhang, Zhun Wang, Shuanghe Peng, Yuanjie Niu, Zhiqun Shang

“…The immune response against prostate cancer was greatly suppressed when the antigen targets were knocked out using CRISPR-Cas9. (4) Conclusion: In summary, our results provide the first evidence that a vaccine based on a fusion protein consisting of Ag85B and a prostate cancer octapeptide epitope with complete Freund’s adjuvant (CFA), triggers a robust immune response and inhibits tumor growth in murine prostate cancer.…”
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The Mutation of the DNA-Binding Domain of Fur Protein Enhances the Pathogenicity of <i>Edwardsiella piscicida</i> via Inducing Overpowering Pyroptosis by Mimi Niu, Zhihai Sui, Guoquan Jiang, Ling Wang, Xuemei Yao, Yonghua Hu

“…To further reveal the regulatory mechanism of Fur, the DNA-binding domain (N-terminal) of Fur was knocked out in this study and the mutant was named TX01Δ<i>fur</i>2. …”
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TPD52L2 as a potential prognostic and immunotherapy biomarker in clear cell renal cell carcinoma by Hongbo Wang, Zhendong Liu, Yuelin Du, Xingbo Cheng, Shanjun Gao, Yanzheng Gao, Panfeng Shang

“…Finally, in vitro experiments confirmed that knocking down TPD52L2 can inhibit the proliferation, migration, and invasion abilities of ccRCC cells.ConclusionThis study for the first time revealed the upregulation of TPD52L2 expression in ccRCC, which is closely associated with poor prognosis of patients and is a potentially valuable therapeutic and efficacy assessment target for immunotherapy.…”
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