Double CRISPR knockout of pectin degrading enzymes improves tomato shelf‐life while ensuring fruit quality by Isabel Ortega‐Salazar, Daphne Crum, Adrian O. Sbodio, Yuko Sugiyama, Adam Adaskaveg, Duoduo Wang, Graham B. Seymour, Xueqi Li, Selina C. Wang, Barbara Blanco‐Ulate

“…Improving fruit firmness in tomato fresh‐market varieties directly impacts their shelf‐life potential. We simultaneously knocked out two pectin‐degrading enzymes, polygalacturonase (SlPG2a) and pectate lyase (SlPL), key for tomato fruit softening. …”
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Paraoxonase 2 Deficiency Causes Mitochondrial Dysfunction in Retinal Pigment Epithelial Cells and Retinal Degeneration in Mice by Parameswaran Gangadharan Sreekumar, Feng Su, Christine Spee, Elise Hong, Ravikiran Komirisetty, Eduardo Araujo, Steven Nusinowitz, Srinivasa T. Reddy, Ram Kannan

“…Mitochondrial localization and regulation of PON2 following oxidative stress were determined in human primary cultured retinal pigment epithelium (hRPE) cells. PON2 was knocked down in RPE cells using siRNA and mitochondrial bioenergetics were measured. …”
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Effect of Stress on Irradiation Responses of Highly Oriented Pyrolytic Graphite by Zhihan Hu, Di Chen, SeungSu Kim, Rijul Chauhan, Yongchang Li, Lin Shao

“…MD simulations of carbon knock-on at energies < 60 eV were used to obtain average threshold displacement energies (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mover accent="true"><mi>E</mi><mo>¯</mo></mover><mi>d</mi></msub></mrow></semantics></math></inline-formula>) as a function of strain ranging from 0 to 10%. …”
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CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells by Hong Qin, Chenglu Sun, Dejun Kong, Yanglin Zhu, Bo Shao, Shaohua Ren, Hongda Wang, Jingyi Zhang, Yini Xu, Hao Wang

“…The present study was conducted to investigate whether the expression of CD73 on ERCs is critical for its therapeutic effects in Con A-induced hepatitis. Methods ERCs knocking out CD73 were generated with lentivirus-mediated CRISPR-Cas9 technology and identified by flow cytometry, western blot and AMPase activity assay. …”
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A Co-Expressed Natural Antisense RNA FCER1A-AS Controls IgE-Dependent Immunity by Promoting Expression of FcεRIα by Ruo-yu Tang, Lan Yin, Liang Yao, Qing-Feng Zhang, Xiao-Ping Chen

“…When FCER1A-AS is selectively knocked down by the CRISPR/RfxCas13d (CasRx) approach in MC/9 cells, the expression of both FCER1A-S mRNA and proteins is markedly decreased. …”
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Control of Grain Weight and Size in Rice (Oryza sativa L.) by OsPUB3 Encoding a U-Box E3 Ubiquitin Ligase by Shi-Lin Wang, Zhen-Hua Zhang, Ye-Yang Fan, De-Run Huang, Yao-Long Yang, Jie-Yun Zhuang, Yu-Jun Zhu

“…The effects were confirmed firstly by using CRISPR/Cas9-based mutagenesis and then through transgenic complementation of a Cas9-free knock-out (KO) mutant. Two homozygous KO lines were produced, each having a 1-bp insertion in OsPUB3 which caused frameshift mutation and premature termination. …”
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Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted Bollworm <i>Earias vittella</i> (Lepidoptera: Nolidae) by Shelja Sandal, Satnam Singh, Gulshan Bansal, Ramandeep Kaur, Kanakachari Mogilicherla, Suneet Pandher, Amit Roy, Gurmeet Kaur, Pankaj Rathore, Anu Kalia

“…A total of six genes including <i>Juvenile hormone methyl transferase</i> (<i>JHAMT</i>)<i>, Chitin synthase</i> (<i>CHS</i>)<i>, Aminopeptidase</i> (<i>AMN</i>)<i>, Cadherin</i> (<i>CAD</i>)<i>, Alpha-amylase</i> (<i>AMY</i>), and <i>V-type proton ATPase</i> (<i>V-ATPase</i>) were selected and knocked down significantly with three different nanoparticles encapsulated dsRNA conjugates, i.e., Chitosan-dsRNA, carbon quantum dots-dsRNA (CQD-dsRNA), and Lipofectamine-dsRNA conjugate. …”
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Golgi-associated retrograde protein (GARP) complex-dependent endosomes to trans Golgi network retrograde trafficking is controlled by Rab4b by Jérôme Gilleron, Abderrahman Chafik, Sandra Lacas-Gervais, Jean-François Tanti, Mireille Cormont

“…Methods We studied the retrograde trafficking to the trans-Golgi network by using fluorescent cargoes in cells overexpressing Rab4b or after Rab4b knocked-down by small interfering RNA in combination with the downregulation of subunits of the Golgi-associated retrograde protein complex. …”
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TNF-α/IL-1β-licensed hADSCs alleviate cholestatic liver injury and fibrosis in mice via COX-2/PGE2 pathway by Xiaoyu Luan, Peng Chen, Yaxin Li, Xinying Yuan, Longyu Miao, Pengyu Zhang, Qilong Cao, Xiaomin Song, Guohu Di

“…Small interfering RNA (siRNA) was used to knock down cyclooxygenase-2 (COX-2) in hADSCs. Results TNF-α/IL-1β preconditioning could downregulate immunogenic gene expression and enhance the engraftment efficiency of hADSCs. …”
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RAB6B is a potential prognostic marker and correlated with the remolding of tumor immune microenvironment in hepatocellular carcinoma by Hao Peng, Erwei Zhu, Jitao Wang, Jitao Wang, Xuanlong Du, Chonggao Wang, Meng Yang, Yewei Zhang

“…Drug sensitivity was analyzed through the GDSC and CTRP databases. RAB6B was knocked down with siRNA in HCC cell lines. EdU assay was performed to detect the cell proliferation ability, flow cytometry was used to compare the differences in the ability of apoptosis, and MTT was used to evaluate the drug sensitivity in vitro.Results: RAB6B mRNA and protein expression were upregulated in the HCC tissues. …”
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Development and implementation of a Type I-C CRISPR-based programmable repression system for Neisseria gonorrhoeae by Wendy E. Geslewitz, Amaris Cardenas, Xufei Zhou, Yan Zhang, Alison K. Criss, H Steven Seifert

“…Importantly, we also show that we can use CRISPRi to knockdown genes that are essential to Gc that cannot normally be knocked out under laboratory settings. Gc encodes ~800 essential genes, many of which have no predicted function. …”
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Biliverdin modulates the Nrf2/A20/eEF1A2 axis to alleviate cerebral ischemia-reperfusion injury by inhibiting pyroptosis by Wenya Bai, Siying Huo, Guilin Zhou, Junjie Li, Yuan Yang, Jianlin Shao

“…Our studies have also demonstrated that either the knock-down of A20 or over-expression of eEF1A2 blunted the protective effect of Biliverdin. …”
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Inhibition of SRPK1, a key splicing regulator, exhibits antitumor and chemotherapeutic-sensitizing effects on extranodal NK/T-cell lymphoma cells by Cuiying He, Beichen Liu, Huan-You Wang, Lili Wu, Guimin Zhao, Chen Huang, Yueping Liu, Baoen Shan, Lihong Liu

“…Methods SRPK1 expression was measured in 41 ENKTL patients by immunohistochemistry and mRNA expression was analyzed by qRT‒PCR. We knocked down SRPK1 expression in the ENKTL cell line YT by siRNA transfection and inhibited SRPK1 using inhibitors (SPHINX31 and SRPIN340) in YT cells and peripheral blood lymphocytes (PBLs) isolated from ENKTL patients to investigate its role in cell proliferation and apoptosis. …”
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The Major Ciliary Isoforms of RPGR Build Different Interaction Complexes with INPP5E and RPGRIP1L by Christine Vössing, Paul Atigbire, Jannis Eilers, Fenja Markus, Knut Stieger, Fei Song, John Neidhardt

“…<i>RPGR^ex1-19</i> and <i>RPGR^ORF15</i> were knocked out. Immunocytochemistry demonstrated that the RPGR^skip14/15 protein localizes along primary cilia, resembling the expression pattern of RPGR^ex1-19. …”
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Construction of a fur null mutant and RNA-sequencing provide deeper global understanding of the Aliivibrio salmonicida Fur regulon by Sunniva Katharina Thode, Cecilie Bækkedal, Jenny Johansson Söderberg, Erik Hjerde, Hilde Hansen, Peik Haugen

“…To provide a more accurate and deeper global understanding of the biological role of Fur we have now generated an A. salmonicida fur knock-out strain and used RNA-sequencing to compare gene expression between the wild-type and fur null mutant strains. …”
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Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata by Germano Sollazzo, Katerina Nikolouli, Georgia Gouvi, Roswitha A. Aumann, Marc F. Schetelig, Kostas Bourtzis

“…Its location was confirmed on the right arm of chromosome 5 in the putative tsl genomic region. Knock-out of Ccdor using CRISPR/Cas9-NHEJ and targeting the fourth exon resulted in lethality at mid- and late-pupal stage, while the successful application of CRISPR HDR introducing a point mutation on the sixth exon resulted in the establishment of the desired strain and two additional strains (dor 12del and dor 51dup), all of them expressing tsl phenotypes and presenting no (or minimal) fitness cost when reared at 25 °C. …”
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Whole Genome Microarray Analysis of DUSP4-Deletion Reveals A Novel Role for MAP Kinase Phosphatase-2 (MKP-2) in Macrophage Gene Expression and Function by Thikryat Neamatallah, Shilan Jabbar, Rothwelle Tate, Juliane Schroeder, Muhannad Shweash, James Alexander, Robin Plevin

“…Methods: We investigated the impact of MKP-2 in the regulation of several genes that are involved in function while using comparative whole genome microarray analysis in macrophages from MKP-2 wild type (wt) and knock out (ko) mice. Results: Our data showed that the lack of MKP-2 caused a significant down-regulation of colony-stimulating factor-2 (<i>Csf2</i>) and monocyte to macrophage-associated differentiation (<i>Mmd</i>) genes, suggesting a role of MKP-2 in macrophage development. …”
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PTEN regulates invasiveness in pancreatic neuroendocrine tumors through DUSP19-mediated VEGFR3 dephosphorylation by Tsung-Ming Chang, Pei-Yi Chu, Hui-You Lin, Kuo-Wei Huang, Wen-Chun Hung, Yan-Shen Shan, Li-Tzong Chen, Hui-Jen Tsai

“…In this study, we investigated the function of PTEN on regulation of pNET invasiveness and its association with VEGFR3 and DUSP19. Methods PTEN was knocked down or overexpressed in pNET cells to evaluate its effect on invasiveness and its association with VEGFR3 phosphorylation. …”
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Role of microRNAs and their downstream target transcription factors in zebrafish thrombopoiesis by Ayah Al Qaryoute, Weam Fallatah, Sanchi Dhinoja, Revathi Raman, Pudur Jagadeeswaran

“…In our laboratory, we identified 15 microRNAs in thrombocytes using single-cell RNA sequencing. We knocked down each of these 15 microRNAs by the piggyback method and found knockdown of three microRNAs, mir-7148, let-7b, and mir-223 in adult zebrafish led to an increase in the percentage of thrombocytes. …”
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A novel αB-crystallin R123W variant drives hypertrophic cardiomyopathy by promoting maladaptive calcium-dependent signal transduction by Chun Chou, Gregory L. Martin, Gayani Perera, Junya Awata, Amy Larson, Robert Blanton, Robert Blanton, Michael T. Chin, Michael T. Chin

“…Here, we generated mice carrying the CryabR123W knock-in allele and demonstrated that hearts from these animals exhibit increased maximal elastance at young age but reduced diastolic function with aging. …”
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