Centromere protein N may be a novel malignant prognostic biomarker for hepatocellular carcinoma by Qingqing Wang, Xiaoyan Yu, Zhewen Zheng, Fengxia Chen, Ningning Yang, Yunfeng Zhou

“…Further cell experiments showed that knocking down CENPN expression decreased the proliferation and colony-forming abilities of HepG2 and Huh7 cells as well as Ki67 expression in these cell lines. …”
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Micropeptide MIAC inhibits the tumor progression by interacting with AQP2 and inhibiting EREG/EGFR signaling in renal cell carcinoma by Mengwei Li, Guangxiang Liu, Xinrong Jin, Hongqian Guo, Sarra Setrerrahmane, Xindi Xu, Tiantian Li, Yunfei Lin, Hanmei Xu

“…Overexpression of MIAC in renal carcinoma cells can significantly inhibit the proliferation and migration ability, promote apoptosis of renal carcinoma cells, and affect the distribution of cells at various stages. After knocking down MIAC, the trend is reversed. In vivo experiments have found that MIAC overexpression inhibit the growth and metastasis of RCC, while the synthetized MIAC peptides can significantly inhibit the occurrence and development of RCC in vitro and in vivo. …”
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Deciphering the significance of anoikis in bladder cancer and systematic analysis of S100A7 as a potential therapeutic target by Haoran Wang, Jianyong Liu, Runhua Tang, Jie Hu, Ming Liu, Jianye Wang, Jingwen Zhang, Huimin Hou

“…Finally, we identified that knocking down S100A7 gene expression restrained the proliferation and invasion of bladder cancer cells. …”
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Machine learning and experimental validation identified autophagy signature in hepatic fibrosis by Yushen Huang, Wen Luo, Zhijie Yang, Tian Lan, Xiaomou Wei, Hongwen Wu

“…Compared with HC group, HF group showed low positive area in F4/80, IL-17 and CD56, indicating decreased expression of macrophage, Th17 cell, natural killer cell and CD56dim natural killer cell. Meanwhile, knocking down RB1CC1 was found to inhibit the activation of hepatic stellate cells and alleviate liver fibrosis.ConclusionATG5, RB1CC1, and PARK2 are promising autophagy-related therapeutic biomarkers for HF. …”
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APOE4 genotype and aging impair injury-induced microglial behavior in brain slices, including toward Aβ, through P2RY12 by Jordy Sepulveda, Jennifer Yejean Kim, Joseph Binder, Stefano Vicini, G. William Rebeck

“…Using ex vivo confocal microscopy, we analyzed microglial dynamic behaviors in the entorhinal cortex (EC) and hippocampus CA1 of 6-, 12-, and 21-month-old mice APOE3 or APOE4 knock-in mice expressing GFP under the CX3CR1 promoter. …”
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La dimensión del descalabro: Carmen Vincenti ante la fundación de la República Bolivariana de Venezuela Carmen Vicenti facing the foundation of the Bolivarian Republic of Venezuela... by Mariana Libertad Suárez

“…More than of a dialogue, it could be spoken of a symbolic fight that was carried out in the academic spaces as in the production of cultural objects, and that looked for to construct and/or to knock down the structured political speech from the spaces of the authority. …”
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MCO1 and MCO3, two putative ascorbate oxidases with ferroxidase activity, new candidates for the regulation of apoplastic iron excess in Arabidopsis by Alexis Brun, Marija Smokvarska, Lili Wei, Sandrine Chay, Catherine Curie, Stéphane Mari

“…Moreover, cell plasmolysis experiments showed that MCO1 remained closely associated to the plasma membrane whereas MCO3 filled the entire apoplast compartment. Although the four knock out mutant lines isolated (mco1‐1, mco1‐2, mco3‐1, and mco3‐2) did not display any macroscopic phenotype, histochemical staining of Fe with the Perls/DAB procedure revealed that mesophyll cells of all four mutants overaccumulated Fe inside the cells in Fe‐rich structures in the chloroplasts, compared with wild‐type. …”
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Serum lipidomic study of long-chain fatty acids in psoriasis patients prior to and after anti-IL-17A monoclonal antibody treatment by quantitative GC‒MS analysis with in situ extra... by XiaoYu Guo, Jianglu Zhou, Hong Yu, Han Cao, Xia Li, Qing Hu, YunQiu Yu

“…Imiquimod (IMQ)-treated wild type (WT) and T-cell receptor delta chain knock-out (Tcrd −/− ) mice were used to investigate the correlation between IL-17A and abnormal changes in LCFAs in psoriasis patients. …”
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Midkine Is a Potential Therapeutic Target of Tumorigenesis, Angiogenesis, and Metastasis in Non-Small Cell Lung Cancer by Dong Hoon Shin, Jeong Yeon Jo, Sun Ha Kim, Minyoung Choi, Chungyong Han, Beom K. Choi, Sang Soo Kim

“…However, in response to MDK knock down, siRNA or the MDK inhibitor, iMDK treatment not only decreased MDK-induced migration and angiogenesis of endothelial cells but also abrogated the progression and metastasis of NSCLC cells in in vitro and in vivo orthotopic and spontaneous lung metastasis models. …”
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Targeted Knockdown of Macrophage Migration Inhibitory Factor Enhances UVB Irradiation-Induced Apoptosis Via Increasing ROS Generation in Oral Squamous Cell Carcinoma by Tian Chen Master's degree in reading, Qibing Chen Doctor's degree, Fen Li Doctor's degree, Manli Zeng Doctor's degree, Bingru Wang Doctor's degree in reading, Shiyong Huang Doctor's degree in reading, Shiming Chen Doctor's degree, Zezhang Tao Doctor's degree

“…MIF expression in human immortalized oral epithelial cells (HIOEC) and OSCC cells was detected by western blotting. MIF was knocked down or overexpressed in OSCC cell lines (SCC-25 and CAL-27). …”
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Famotidine activates the vagus nerve inflammatory reflex to attenuate cytokine storm by Huan Yang, Sam J. George, Dane A. Thompson, Harold A. Silverman, Téa Tsaava, Aisling Tynan, Valentin A. Pavlov, Eric H. Chang, Ulf Andersson, Michael Brines, Sangeeta S. Chavan, Kevin J. Tracey

“…Either bilateral sub-diaphragmatic vagotomy or genetic knock-out of α7nAChR abolished the anti-inflammatory effects of famotidine, indicating the inflammatory reflex as famotidine’s mechanism of action. …”
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Bone marrow mesenchymal stem cell-derived exosomal miR-30e-5p ameliorates high-glucose induced renal proximal tubular cell pyroptosis by inhibiting ELAVL1 by Jia Lv, Ya-Ning Hao, Xiao-Pei Wang, Wan-Hong Lu, Li-Yi Xie, Dan Niu

“…ELAVL1 was a target of miR-30e-5p and knocking down ELAVL1 reversed the effect of miR-30e-5p inhibition in BMSC-exos-treated HK-2 cells.Conclusions BMSC-derived exosomal miR-30e-5p inhibits caspase-1-mediated pyroptosis by targeting ELAVL1 in HG-induced HK-2 cells, which might provide a new strategy for treating DKD.…”
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Curcumin reverses erastin-induced chondrocyte ferroptosis by upregulating Nrf2 by Yizhao Zhou, Zhen Jia, Jing Wang, Shu Huang, Shu Yang, Sheng Xiao, Duo Xia, Yi Zhou

“…The ferroptosis-related proteins, such as SLC7A11, GPX4, TFR1, ACSL4, and FTH1, were examined using immunofluorescence and western blotting. Nrf2 was knocked down using siRNA to explore the molecular mechanism through which curcumin protects chondrocytes from erastin-induced ferroptosis. …”
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Genome-wide identification of the mitogen-activated kinase gene family from Limonium bicolor and functional characterization of LbMAPK2 under salt stress by Caixia Zhang, Zhihui Zhu, Aijuan Jiang, Qing Liu, Min Chen

“…We explored the function of LbMAPK2 via virus-induced gene silencing: knocking down LbMAPK2 transcript levels in L. bicolor resulted in fewer salt glands, lower salt secretion ability from leaves, and decreased salt tolerance. …”
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Fast-tracking antibody maturation using a B cell-based display system by Hitomi Masuda, Atsushi Sawada, Shu-ichi Hashimoto, Kanako Tamai, Ke-Yi Lin, Naoto Harigai, Kohei Kurosawa, Kunihiro Ohta, Hidetaka Seo, Hiroshi Itou

“…These results demonstrated the versatility of the novel B cell-based affinity maturation system as an easy-to-use antibody optimization tool regardless of the species of origin.Abbreviations: ADLib®: Autonomously diversifying library, ADLib® KI-AMP: ADLib® knock-in affinity maturation platform, AID: activation-induced cytidine deaminase, CDRs: complementary-determining regions, DIVAC: diversification activator, ECD: extracellular domain, FACS: fluorescence-activated cell sorting, FCM: flow cytometry, HC: heavy chainIg: immunoglobulin, LC: light chain, NGS: next-generation sequencing, PBD: pyrrolobenzodiazepine, SHM: somatic hypermutation, SPR: surface plasmon resonance…”
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Computational study of the motor neuron protein KIF5A to identify nsSNPs, bioactive compounds, and its key regulators by Rupesh Kumar, Thirumurthy Madhavan, Kalaiarasan Ponnusamy, Honglae Sohn, Shazia Haider

“…With the network topological parameters of PPIN (Degree, Bottleneck, Closeness, and MNC) using CytoHubba and computational knock-out experiment using Network Analyzer, we found KIF1A, 5B, and 5C were the significant proteins. …”
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Chicken Glycogen Synthase Kinase 3β Suppresses Innate Immune Responses and Enhances Avian Leukosis Virus Replication in DF-1 Cells by Zhouhao Cui, Bei Weng, Yongxiu Yao, Hongxia Shao, Jianqiang Ye, Aijian Qin, Kun Qian

“…Conversely, levels of most of the genes detected in this study were increased when chGSK3β expression was knocked down using small interfering RNA (siRNA), which also inhibited the replication of ALV-J. …”
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A Cataract-Causing Mutation in the TRPM3 Cation Channel Disrupts Calcium Dynamics in the Lens by Yuefang Zhou, Thomas M. Bennett, Philip A. Ruzycki, Zhaohua Guo, Yu-Qing Cao, Mohammad Shahidullah, Nicholas A. Delamere, Alan Shiels

“…Here, we model the pathogenetic effects of this cataract-causing mutation using ‘knock-in’ mutant mice and human cell lines. <i>Trpm3</i> and its intron-hosted micro-RNA gene (<i>Mir204</i>) were strongly co-expressed in the lens epithelium and other non-pigmented and pigmented ocular epithelia. …”
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Low-dose lipopolysaccharide inhibits spinal cord injury-induced neuronal apoptosis by regulating autophagy through the lncRNA MALAT1/Nrf2 axis by Jianhua Hu, Kun Huang, Feilong Bao, Shixiao Zhong, Qianbo Fan, Weichao Li

“…In vitro SCI model cells were pretreated with LPS and transfected with siMALAT1 or MALAT1 overexpression plasmid aimed at knocking down MALAT1 or overexpressing MALAT1. The cell counting kit-8 (CCK-8) assay was used to measure the toxicity of LPS towards PC12 cells. …”
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The microtubule network enables Src kinase interaction with the Na,K-ATPase to generate Ca2+ flashes in smooth muscle cells by Salomé Rognant, Violetta V. Kravtsova, Elena V. Bouzinova, Elizaveta V. Melnikova, Igor I. Krivoi, Sandrine V. Pierre, Christian Aalkjaer, Thomas A. Jepps, Vladimir V. Matchkov

“…The Na,K-ATPase α2 isoform was knocked down with siRNA and the microtubule network was disrupted with nocodazole. …”
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