Identification and Validation of Hub Immune-Related Genes in Non-Alcoholic Fatty Liver Disease by Li J, Kou C, Sun T, Liu J, Zhang H

“…PCR, oil red O staining and triglyceride detection revealed that the knock-down of BACH2 and S100A9 reduced lipid accumulation in NAFLD cells.Conclusion: This study provided insight into the profile of immune infiltration underlying NAFLD and identified AQP9, BACH2, CD4, IL17RE and S100A9 as ancillary diagnostic indicators of NAFLD. …”
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The effect of an exopolysaccharide probiotic molecule from Bacillus subtilis on breast cancer cells by Mai R. Nguyen, Mai R. Nguyen, Emily Ma, Emily Ma, Debra Wyatt, Katherine L. Knight, Clodia Osipo, Clodia Osipo

“…IKKβ or STAT1 signaling was assessed using pharmacologic or RNAi-mediated knock down approaches. ResultsShort-term treatment with EPS inhibited proliferation of certain breast cancer cells (T47D, MDA-MB-468, HCC1428, MDA-MB-453) while having little effect on others (MCF-7, MDA-MB-231, BT549, ZR-75-30). …”
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miR-124-3p delivered by exosomes from heme oxygenase-1 modified bone marrow mesenchymal stem cells inhibits ferroptosis to attenuate ischemia–reperfusion injury in steatotic grafts... by Longlong Wu, Xuan Tian, Huaiwen Zuo, Weiping Zheng, Xiang Li, Mengshu Yuan, Xiaorong Tian, Hongli Song

“…In addition, HM-exos from cell knocked out for miR-124-3p showed a weakened inhibitory effect on ferroptosis. …”
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PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A by Weidong Lin, Kunpeng Wang, Jinggang Mo, Liezhi Wang, Zhenshun Song, Hao Jiang, Cong Wang, Chong Jin

“…Methods We determined the expression of PIK3R3 in liver cancer by using TCGA data and our clinical samples and knocked it down by siRNA or overexpressing it by the lentivirus vector system. …”
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A novel partitivirus conferring hypovirulence by affecting vesicle transport in the fungus Colletotrichum by Jun Zi Zhu, Ze Lan Qiu, Bi Da Gao, Xiao Gang Li, Jie Zhong

“…When the Rab gene CaRab7, which is associated with endocytosis in vesicle transport, was knocked out, the virulence of the mutants was reduced. …”
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<i>Mamestra brassicae</i> Multiple Nucleopolyhedroviruses Prevents Pupation of <i>Helicoverpa armigera</i> by Regulating Juvenile Hormone Titer by Yanqing Yang, Jinping Dai, Guozhi Zhang, Deepali Singh, Xiaoxia Zhang, Zhenpu Liang

“…We further proved that the JH is degraded by JHE in <i>H. armigera</i> larvae by RNAi, ELISA, RT-qPCR and bioassay, while the hydrolysis of JH by JHEH in <i>H. armigera</i> larvae can almost be ignored. Knocking down of <i>HaJHE</i> promoted the expression of the JH receptor gene <i>Met</i> and the downstream gene <i>Kr-h1</i>, and the replication of MbMNPV. …”
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High LYRM4-AS1 predicts poor prognosis in patients with glioma and correlates with immune infiltration by Hai yue Wang, Ying Xie, Hongzhen Du, Bin Luo, Zengning Li

“…Finally, assays for cell apoptosis, cell viability and wound healing were conducted to evaluate the function on U87 MG and U251 cells after knocking down LYRM4-AS1. Results We found that LYRM4-AS1 was upregulated and related to the grade and malignancy of glioma. …”
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Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway by Mai L, He G, Chen J, Zhu J, Chen S, Yang H, Zhang M, Hou X, Ke M, Li X

“…The PFN1 gene was overexpressed or knocked-down to investigate its function in DN. Flow cytometry was used to detect cell proliferation and apoptosis. …”
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3D Chromatin Architecture Re-Wiring at the <i>CDH3/CDH1</i> Loci Contributes to E-Cadherin to P-Cadherin Expression Switch in Gastric Cancer by Celina São José, Carla Pereira, Marta Ferreira, Ana André, Hugo Osório, Irene Gullo, Fátima Carneiro, Carla Oliveira

“…CRISPR-Cas9 was used to knock out <i>CDH1</i> and a putative regulatory element. …”
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ST14 interacts with TMEFF1 and is a predictor of poor prognosis in ovarian cancer by Xin Nie, Lingling Gao, Mingjun Zheng, Shuang Wang, Caixia Wang, Xiao Li, Ouxuan Liu, Rui Gou, Juanjuan Liu, Bei Lin

“…TMEFF1 expression was also reduced after knocking down ST14 through Western blot. MTT, wound healing and Transwell assays results determined that knockdown of ST14 inhibited proliferation, migration and invasion of ovarian cancer cells in vitro, but the inhibitory effect was restored after adding TMEFF1 exogenous protein. …”
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14-3-3 proteins regulate cullin 7-mediated Eag1 degradation by Chang-Heng Hsieh, Chia-Cheng Chou, Ya-Ching Fang, Po-Hao Hsu, Yi-Hung Chiu, Chi-Sheng Yang, Guey-Mei Jow, Chih-Yung Tang, Chung-Jiuan Jeng

“…Difopein diminished Cul7-mediated Eag1 degradation, and Cul7 knock-down abolished the effect of difopein on Eag1. …”
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Multi-Omics Analysis Reveals the Potential Effects of Maternal Dietary Restriction on Fetal Muscle Growth and Development by Xinyue Wang, Mingyu Shang, Wenping Hu, Li Zhang

“…The multi-omics analysis revealed that the <i>TEAD1</i> gene was in the Hippo signaling pathway, the muscle marker genes <i>MYF5</i> and <i>MyoG</i> were significantly down-regulated, and the <i>TEAD1</i> binding of the down-regulated <i>VGLL3</i> gene might be potential mechanisms affecting myofiber differentiation and maturation. Knocking down the <i>CDK2</i> gene could inhibit the proliferation of primary embryonic myoblasts, and the expression levels of cell cycle regulatory factors <i>CDK4</i> and <i>CDK6</i> were significantly changed. …”
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Systemic Analyses of Cuproptosis-Related lncRNAs in Pancreatic Adenocarcinoma, with a Focus on the Molecular Mechanism of LINC00853 by Leifeng Chen, Lin Zhang, Haihua He, Fei Shao, Yibo Gao, Jie He

“…The differential expressions of LINC00853 between normal tissue and PAAD samples were validated by qRT-PCR. LINC00853 was knocked down by siRNA in PC cell lines BxPC-3 and PANC-1 and the oncogenic role of LINC00853 was validated by CCK8, colony formation, and EdU assays. …”
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Author Correction: Epidemiological drivers of transmissibility and severity of SARS-CoV-2 in England by Pablo N. Perez-Guzman, Edward Knock, Natsuko Imai, Thomas Rawson, Yasin Elmaci, Joana Alcada, Lilith K. Whittles, Divya Thekke Kanapram, Raphael Sonabend, Katy A. M. Gaythorpe, Wes Hinsley, Richard G. FitzJohn, Erik Volz, Robert Verity, Neil M. Ferguson, Anne Cori, Marc Baguelin

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Overcoming insecticide resistance in Anopheles mosquitoes by using faster-acting solid forms of deltamethrin by Jessica Carson, Bryan Erriah, Stephania Herodotou, Alexander G. Shtukenberg, Leilani Smith, Svetlana Ryazanskaya, Michael D. Ward, Bart Kahr, Rosemary Susan Lees

“…Results In all cases, D-Fense Dust heated to generate the more active form of deltamethrin was substantially more effective than the commercially available formulation. 100% of both Banfora M and Kisumu populations were knocked down 10 min post-exposure with no recovery afterwards. …”
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Démocratie, institution, marché. Les vents contraires de la médiation (Democracy, institution, market: the conflicting winds of mediation) by Jacques Faget

“…<br /><br /> The considerable development of mediations over the past few decades in Western countries is correlated with the insufficiencies and anachronism of institutional and social regulations knocked over by postmodernity. The metamorphoses generated by their success are not without struggle between jurists weakened by the crisis of legal rationality, defending their monopoly, and the new professionals of mediation coming from social sciences. …”
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Exosome Derived from Mesenchymal Stem Cells Alleviates Hypertrophic Scar by Inhibiting the Fibroblasts via TNFSF-13/HSPG2 Signaling Pathway by Zhang H, Zang C, Zhao W, Zhang L, Liu R, Feng Z, Wu J, Cui R

“…Through the above mechanisms, knocking out TNFSF13 can inhibit the proliferation, migration, fibrosis and inflammatory response of HSF, whereas MSC-exo could reverse this process. …”
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UBE2S and UBE2C confer a poor prognosis to breast cancer via downregulation of Numb by Yanjing Guo, Yanjing Guo, Xinyu Chen, Xinyu Chen, Xiaowei Zhang, Xiaowei Zhang, Xichun Hu, Xichun Hu

“…In BC cell lines, UBE2S/UBE2C overexpression decreased the level of Numb and enhanced cell malignancy, while knocking down UBE2S/UBE2C demonstrated the opposite effects.ConclusionUBE2S and UBE2C downregulated Numb and enhanced BC malignancy. …”
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A novel necroptosis-related long noncoding RNA model for predicting clinical features, immune characteristics, and therapeutic response in clear cell renal cell carcinoma by Lei Zhang, Lei Zhang, Yongquan Chen, Yongquan Chen, Weijing Hu, Bo Wu, Linfeng Ye, Dongwen Wang, Dongwen Wang, Tao Bai

“…Finally, the expression of NRLs included in the model was verified, and knocking down these NRLs in tumor cells affected cell proliferation, migration, and invasion.ConclusionNecroptosis plays an important role in the progression of ccRCC. …”
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Defective Biomechanics and Pharmacological Rescue of Human Cardiomyocytes with Filamin C Truncations by Marco Lazzarino, Michele Zanetti, Suet Nee Chen, Shanshan Gao, Brisa Peña, Chi Keung Lam, Joseph C. Wu, Matthew R. G. Taylor, Luisa Mestroni, Orfeo Sbaizero

“…CRISPR/Cas9 genome-edited homozygous FLNC^KO−/− hiPSC-CMs and heterozygous knock-out FLNC^KO+/− hiPSC-CMs were analyzed and compared to wild-type FLNC (FLNC^WT) hiPSC-CMs. …”
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